LOCUS       NM_001081356            4195 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
            (Vma21), transcript variant 2, mRNA.
ACCESSION   NM_001081356 XM_898831
VERSION     NM_001081356.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Cocchiararo,I., Cattaneo,O., Rajendran,J., Chabry,F., Cornut,M.,
            Soldati,H., Bigot,A., Mamchaoui,K., Gibertini,S., Bouche,A.,
            Ham,D.J., Laumonier,T., Prola,A. and Castets,P.
  TITLE     Identification of a muscle-specific isoform of VMA21 as a potent
            actor in X-linked myopathy with excessive autophagy pathogenesis
  JOURNAL   Hum Mol Genet 32 (24), 3374-3389 (2023)
   PUBMED   37756622
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ071023.1, AK164163.1, CX564875.1, AL807394.10 and BG143407.1.
            
            On Mar 21, 2014 this sequence version replaced NM_001081356.2.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' coding region and initiates translation at an alternate
            upstream start codon, compared to variant 1. The encoded isoform
            (2) has a distinct N-terminus and is shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK164163.1, AK045401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                CJ071023.1         1-46
            47-3256             AK164163.1         2-3211
            3257-3652           CX564875.1         456-851
            3653-3659           AL807394.10        60728-60734
            3660-4195           BG143407.1         3-538               c
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 37.07 cM"
     gene            1..4195
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)"
                     /db_xref="GeneID:67048"
                     /db_xref="MGI:MGI:1914298"
     exon            1..183
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..436
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     vacuolar ATPase assembly integral membrane protein Vma21"
                     /codon_start=1
                     /product="vacuolar ATPase assembly integral membrane
                     protein Vma21 isoform 2"
                     /protein_id="NP_001074825.1"
                     /db_xref="CCDS:CCDS41000.1"
                     /db_xref="GeneID:67048"
                     /db_xref="MGI:MGI:1914298"
                     /translation="MERLDKAALNALQPPEFRNENSLAATLKTLLFFTALMITVPIGL
                     YFTTKAYIFEGALGMSNRDSYFYAAIVAVVAVHVVLALFVYVAWNEGSRQWREGKQD"
     exon            184..293
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            294..4195
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      522..527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      550
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="major polyA site"
     regulatory      4171..4176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4195
                     /gene="Vma21"
                     /gene_synonym="2610030H06Rik"
                     /note="major polyA site"
ORIGIN      
        1 tacgccgcgc cacgcgagcg tgcttcggca ggggcacttc cggcgcgacc ggcctagact
       61 gtcaccctgt tagccgccgc tgagcctccg ctaactaccc ggcgcctgct gctgccctcc
      121 cggccacgcc atggagcgtc ttgataaagc agcgctcaat gcgctgcagc ctccagagtt
      181 cagaaatgaa aactctttag ctgcaaccct gaagacactg ctgttcttca cagctttaat
      241 gatcacggtt cctatcggct tgtattttac aacgaaagct tatatatttg aaggtgccct
      301 tggaatgtct aatagagaca gctattttta tgccgcaatt gttgctgttg ttgctgttca
      361 tgtggttctg gccctgtttg tctatgtggc ctggaatgaa ggctcacgac agtggcgtga
      421 aggcaaacag gattaaagtg acatcacctt ttgatagcat gaaatttatt tttgaatatg
      481 ttacataatt ccggggtatt gataataaat acatttgttt gaataaagtt gaaagaacat
      541 gttaaaatca gtcttaaaga gtcagtcaca tttggctaaa ttttgaccca cccaataata
      601 tggttacttt gaactttgaa tctccatttg caaatgaaaa tgtggaaaag ttggagaggt
      661 acagatgtat atgtttcatg agttatctct tatcctaggg agaatttaca tatcttacaa
      721 taacaaaaat gatagcaatg tggcttatac ttgcttttca gttttattta tttgggtata
      781 ttaagaaata cttcatgaag caaatctaaa ttcccttttt tactgaagga cttcgctgga
      841 attaccaggc tcttgggttt gagattctac tttccttaaa agttacttac attgtaggca
      901 ttttagttaa tctgactttt aagtcagtga ggaaagattg ctctcacatt ccattatttc
      961 acagggaaag tcactgaaac cacactattc atgcaaaagg gtgtagggtt actgaggaca
     1021 gccaactggg ctgactgcca gcattgtgaa tatcacacta tcattgtgcc ctcactttgc
     1081 tttgaaatta ggtttcagtt gcaaagttta tcgaaattcc acttaatttg ttgttctagg
     1141 ttaaaatagt tttagaatac caaaatgttc agcatttaca aattgagagt ctatacaggc
     1201 ttgaaacatc acaattctta tgaggtgttc atgaagttgt tttgtctatt aatccttttt
     1261 ttaaatgaca tctcagctac atcctgagca taagctatgt tctaagataa tccttagagt
     1321 ctttttttga ttagaacaac cctgcgcctg aaaaaaagcc cagttccctt ggtaccttca
     1381 gtctctggtc agacagacct tttggtactg tgtatgtgta taaaaacgac gacccccaaa
     1441 tatttatttc tgattaaaag ataatatatt ctggaaaaca ctggaaaata catataaaaa
     1501 ttaaaaaagc aaaaaatttc ccataatgtt aactacctgc agattatcag tagtttagtt
     1561 cacaacccct gtacaagtac actaaaacct tgttttaaat ttagatcttg tcaacttcac
     1621 caaagagctg tttgcagttt ttcagatgtg tggtaattgc ccattgcctt acaaagtata
     1681 tactatcttt attattttaa aattcccatt tcaatgtttt caccataaag aaatgtaaaa
     1741 atttgaggtg ataacctagt ttaaatacta tataacatgc atatctcaaa acattacatg
     1801 gcacccataa gcgtgtatgc ttcttgtttt tatgttaatt aaattgaatt gtaaaaatga
     1861 agaaatttca aatacctgtt actgataatc ctaatataat aaatggcccc tgcttaaata
     1921 tttgctatta ttgatttgat ggtctactgt tattttcatg ctgtggaact agactagaaa
     1981 aataggtgat tggatataaa aatatgtgct tgtgttatgt tattgttata aacaacaaga
     2041 ttattgtgat tttaaaaaga ttgttcttaa ttcttaaatt taacagttgt gtgtagttta
     2101 aatatatttt aaaatgagca ttctaaccat gagagtcttt attttgtaaa gactgcagtt
     2161 tttagtatta tgatatttga gtcctagaca ttgtgtgcta gagttctaga gggttggttc
     2221 ccttattagg tggcctatta tatttagaat tgtccactca gtttcttttt atgtaatgcc
     2281 aaggcttttt tcttatactt taggaatatt gtactatttg taaaatatac tgttattttg
     2341 ttttatttca aacgtttgct aactaatggt acaggtcttt taaaaagtat tttacccagg
     2401 gatacatgta aacacatgta attttccctt ggcaataaag tacaatgtgt ttgaccatta
     2461 aaatattcac actggtattt agaattatta taaaacttgt tttaaagcat tttttctagg
     2521 attttgactg cttacttatg agtttgctgc tgttaggtaa cattttttta aggtttttaa
     2581 ctagtttcct ctttcacaaa gtacatattt catgctgtgt tagaagaaaa gctacagtta
     2641 acctgttaag tgttttccgg cggaaactgt tcactgatgg tcaaaacctg attgtaatgg
     2701 tgatcccagt tagggagtat aacttgaagt gtccttgcaa gctactaatt taaccccact
     2761 tgctgtaaca gtacttttcc caacttctga tatgccttcc gaataatcct ttcagtgcta
     2821 aaaaaatgga aggctgctgt aggatgcatc tggtatcact gactctttaa atggatgcca
     2881 tttcacaggt tagagtagca ttctggtttt caatgatagt aaagaatttt aaacctgcta
     2941 ttcaaagtaa ctgcgtattt catgggaatc taatagtaac tataacttga agtttacatg
     3001 agaaaagtca tagtgaaccg gttatggtgt tccattattt taggaagcag atgtaggagg
     3061 attaggagtt taagaccacc ctggacttca tgagacccta tatcaaaaga acagcagcaa
     3121 caacaaaagc tacaaaaaca atttgactta ataataataa aaaaaagcaa ctaatgggct
     3181 tgtgcttatt ttcatataaa tcttttgtag acaggacatt caaatgggta agtgaaagta
     3241 tacaaggaag atgtaaccaa ggaaggctga tgctggaggt gtgcgaaaga aaggcttgag
     3301 atccgtttta catatatcca gtgtatctcc agacagtttg tatttagtgt gtaagtatag
     3361 taaatctgta gacaaagtgt tcagcgatgg tacagtattt gtcacttgtc ttcagttaat
     3421 agcaggtttc tttactacgt tattcactag tgaaacacac agctattcag attttgctcc
     3481 taaaatttat ttttaacaga cttgtgctat agtgtagctt gacaacattt tttatcccag
     3541 gagaatggag ttaccttaaa ataaattact ttcagagata ctggaaattt actcatcaat
     3601 gcataacctg tgaatttctt aactccattc ctttacagac gtctagaatc tttccatatc
     3661 gctgccggcc acacttttaa acctgtgtta attgctatgt gtgcttgaag tatctgttct
     3721 tttaaagatg ttctttaagt catattgtca gttttacaat ctagtcttcc ttccatgttt
     3781 ggtgaaatct cactgaacag gaataatagc aataactaac aacatctgca cagcacctta
     3841 cagtttgcag agaacgttca catattcttg tctgagtttt gcatagtgaa catgtcacag
     3901 agatgtccct tgatgtcaat gctaagtgtt agaatgttca gtcacagtgt cactggcctt
     3961 gctgcggcac tgattagcac actgacgggt ggggggaggg gggcgtgccg atgtgctgtc
     4021 tgtaaagctg cgtcatcaca acggggcctg gatagcttag ccacttgctt cagactgact
     4081 tgttaagtga tgcttttcta ctctacctga atggcttttg tagtcagtat attgtagcct
     4141 gtaatttata ttaatttacc atttgttgtc attaaaaaaa gtgtctgtaa tgcta
//
