LOCUS       NM_001033236            1909 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus valosin containing protein lysine (K)
            methyltransferase (Vcpkmt), transcript variant 2, mRNA.
ACCESSION   NM_001033236 XM_127052 XM_897567 XM_897578 XM_897587 XM_916743
VERSION     NM_001033236.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 1909)
  AUTHORS   Fusser,M., Kernstock,S., Aileni,V.K., Egge-Jacobsen,W., Falnes,P.O.
            and Klungland,A.
  TITLE     Lysine Methylation of the Valosin-Containing Protein (VCP) Is
            Dispensable for Development and Survival of Mice
  JOURNAL   PLoS One 10 (11), e0141472 (2015)
   PUBMED   26544960
  REMARK    GeneRIF: Lysine Methylation of the Valosin-Containing Protein (VCP)
            Is Dispensable for Development and Survival of Mice
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1909)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153658.4.
            
            On Mar 18, 2020 this sequence version replaced NM_001033236.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144832.1, SRR11927938.4196436.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-297               AC153658.4         58204-58500
            298-408             AC153658.4         58591-58701
            409-478             AC153658.4         58876-58945
            479-598             AC153658.4         59984-60103
            599-703             AC153658.4         61211-61315
            704-1909            AC153658.4         63281-64486
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 28.92 cM"
     gene            1..1909
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /note="valosin containing protein lysine (K)
                     methyltransferase"
                     /db_xref="GeneID:207965"
                     /db_xref="MGI:MGI:2684917"
     exon            1..297
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..718
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /note="isoform 2 is encoded by transcript variant 2;
                     methyltransferase-like protein 21D; protein-lysine
                     methyltransferase METTL21D; methyltransferase like 21D;
                     VCP lysine methyltransferase; valosin-containing protein
                     lysine methyltransferase; protein N-lysine
                     methyltransferase METTL21D"
                     /codon_start=1
                     /product="protein N-lysine methyltransferase METTL21D
                     isoform 2"
                     /protein_id="NP_001028408.2"
                     /db_xref="CCDS:CCDS36464.1"
                     /db_xref="GeneID:207965"
                     /db_xref="MGI:MGI:2684917"
                     /translation="MAAAVEPEVEDPLWSFVRVLEKRDGTVLRLQQYGSGGVGCVVWD
                     AAIVLSKYLETPGFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVIVTDLEELQD
                     LLKMNIDMNKHLVTGSVQAKVLKWGEDIEDLMSPDYILMADCIYYEESLEPLLKTLKD
                     LSGSETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEEIPLDKHDEEYRSEDIHIVY
                     IRKKKPKPPS"
     exon            298..408
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            409..478
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            479..598
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            599..703
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            704..1909
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1890..1895
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /note="hexamer: AATAAA"
     polyA_site      1909
                     /gene="Vcpkmt"
                     /gene_synonym="Gm71; Mettl21d; Mettl221d; VCP-KMT"
                     /note="major polyA site"
ORIGIN      
        1 gtgccgcagc ctgcggcggg ccggggcagg gatggcggcc gccgtggagc cggaggtgga
       61 ggacccgctg tggagctttg tgcgggttct ggagaagcgg gatgggacag tgcttcggct
      121 gcagcagtac ggctccgggg gcgtgggttg cgttgtgtgg gacgccgcca tcgtcctttc
      181 caaatacctg gagacgccgg ggttttctgg cgacggggcc cacgcgctga gtcggcgctc
      241 ggtgctggag ctgggctccg gcacgggggc cgtggggctc atggctgcga ccctcggggc
      301 agatgttata gtcactgatc ttgaagagct gcaggacttg ctgaaaatga atattgatat
      361 gaacaagcat cttgtgactg gttctgttca agccaaggta ctgaaatggg gtgaagacat
      421 agaagacttg atgtcaccag actacatatt gatggcagac tgtatatact atgaggagtc
      481 cttggaacca ctgctgaaaa cattaaaaga tctcagtgga tctgaaactt gtattatatg
      541 ttgttacgaa caacgtacaa tggggaaaaa tccagaaatt gagaagaagt attttgagct
      601 cctgcagcta gactttgact ttgaagaaat ccctttggac aaacatgatg aagaataccg
      661 aagtgaagac attcatattg tgtacatcag aaagaaaaag ccaaaaccgc catcatgaag
      721 cgcttctcca tcttagccac gccccgcaac atcctgggta aggtcaagat gtgaacggag
      781 catgtgcata ccgcatggaa gactgcctca cagatttctc cagcatgtcc ttagagatct
      841 ggtacatgga tgacaactac tttggactgc ccacaatgtg aaatgtctgc ctgcccaccc
      901 gcccgcccac ctgtgggctg aaatctaacg taggtttact tagttcggca tcaagttctg
      961 cttaaaagaa acgttgacaa tcactcaaat aaaaattaat tttgagagac cctcaagtct
     1021 gaagcagcat ggttggctca gtacatttct aagaagctca aaatcagttg atccaatttc
     1081 taatggcatc tattactgta caggaatgtt ctgaggactg gtgtcatggt ggcgcacgcc
     1141 tttaatacca gcacagggga gacagaagca ggtggccctc ttgagttcca gaccagcctg
     1201 ctctacgggg caagctggtt ccaagcataa taggactgtc tcgaaggaag gaaggaagga
     1261 aggaaggaag gaaggaagga aggaaggaag gagggaggga gaaaggagca aaaacccaaa
     1321 accatagaga agttgatgca atccagcttc cagtgctagt gtctctcatt atataatact
     1381 aaggtgaagt tcagaggcag tctcactttt acatagttgt gtagtggttt ctctttttcc
     1441 tttgtgttat gtttaatact taacatgttc tttgaaagga aactgaaaat actattttgt
     1501 acctttactg ggcagagttg tgtttccctc catcacaggt aggtgagtga actggggaac
     1561 tggggactag tgaactgggg agtctggggc gctgtagctc taggtcacta agaagataag
     1621 tcaggggacg ggggaccaag cgctgtctca gggaggtgtg aggagagaaa gccagttgca
     1681 ggcttctgct tcatacttaa atggagttct gtgtacattt atcaaatggt ttttactgtg
     1741 ttgtctccaa cttttttgtt gttccaataa tcacctgctc acatagtcta ctgtgtcagt
     1801 cttgagcacc accaccagat ccaaaaagta gtaaccagat gtattctgga gtttacagtc
     1861 ggtgctagat ggcacgttgt atgtgctgaa ataaacaccg tttttgaaa
//
