LOCUS       NM_173443               9749 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus valosin containing protein (p97)/p47 complex
            interacting protein 1 (Vcpip1), mRNA.
ACCESSION   NM_173443
VERSION     NM_173443.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Huang,J., Zhou,Q., Gao,M., Nowsheen,S., Zhao,F., Kim,W., Zhu,Q.,
            Kojima,Y., Yin,P., Zhang,Y., Guo,G., Tu,X., Deng,M., Luo,K.,
            Qin,B., Machida,Y. and Lou,Z.
  TITLE     Tandem Deubiquitination and Acetylation of SPRTN Promotes
            DNA-Protein Crosslink Repair and Protects against Aging
  JOURNAL   Mol Cell 79 (5), 824-835 (2020)
   PUBMED   32649882
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Tsai,Y.C., Kotiya,A., Kiris,E., Yang,M., Bavari,S., Tessarollo,L.,
            Oyler,G.A. and Weissman,A.M.
  TITLE     Deubiquitinating enzyme VCIP135 dictates the duration of botulinum
            neurotoxin type A intoxication
  JOURNAL   Proc Natl Acad Sci U S A 114 (26), E5158-E5166 (2017)
   PUBMED   28584101
  REMARK    GeneRIF: BoNT/A catalytic light chain binds to, and is a substrate
            for, the ubiquitin ligase HECTD2. However, the light chain evades
            proteasomal degradation by the dominant effect of a
            deubiquitinating enzyme, VCIP135/VCPIP1. This deubiquitinating
            enzyme binds BoNT/A light chain directly.
REFERENCE   3  (bases 1 to 9749)
  AUTHORS   Totsukawa,G., Kaneko,Y., Uchiyama,K., Toh,H., Tamura,K. and
            Kondo,H.
  TITLE     VCIP135 deubiquitinase and its binding protein, WAC, in
            p97ATPase-mediated membrane fusion
  JOURNAL   EMBO J 30 (17), 3581-3593 (2011)
   PUBMED   21811234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9749)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   5  (bases 1 to 9749)
  AUTHORS   Dreveny,I., Kondo,H., Uchiyama,K., Shaw,A., Zhang,X. and
            Freemont,P.S.
  TITLE     Structural basis of the interaction between the AAA ATPase p97/VCP
            and its adaptor protein p47
  JOURNAL   EMBO J 23 (5), 1030-1039 (2004)
   PUBMED   14988733
  REMARK    GeneRIF: that p47 ubiquitin regulatory X domain domains may act as
            general p97/VCP/CDC48 binding modules and that adaptor binding for
            N-ethylmaleimide sensitive factor and p97 might involve different
            binding sites
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC059209.1 and AC158790.5.
            
            On Dec 16, 2014 this sequence version replaced NM_173443.2.
            
            Summary: This gene encodes a deubiquitinating enzyme that interacts
            with valosin containing protein p97 and plays a role in the
            assembly of Golgi apparatus during mitosis. [provided by RefSeq,
            Dec 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059209.1, AK122563.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5179              BC059209.1         1-5179
            5180-9749           AC158790.5         147655-152224       c
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.08 cM"
     gene            1..9749
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="valosin containing protein (p97)/p47 complex
                     interacting protein 1"
                     /db_xref="GeneID:70675"
                     /db_xref="MGI:MGI:1917925"
     exon            1..2933
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..3889
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /EC_number="3.4.19.12"
                     /note="deubiquitinating protein VCIP135;
                     valosin-containing protein p97/p47 complex-interacting
                     protein p135; VCP/p47 complex-interacting 135-kDa protein"
                     /codon_start=1
                     /product="deubiquitinating protein VCPIP1"
                     /protein_id="NP_775619.2"
                     /db_xref="CCDS:CCDS14814.1"
                     /db_xref="GeneID:70675"
                     /db_xref="MGI:MGI:1917925"
                     /translation="MSQPPPPPPLPPPPPPPEAPQTSSSLAAAASPGGLSKRRDRRIL
                     SGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNAL
                     LGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCA
                     LLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHC
                     LVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLF
                     VPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGR
                     DGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEE
                     DGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPE
                     EVTAAAKKAVMDNRLHKCLLCGALSELHVPSEWLAPGGKLYNLAKSTHGQLRPDKNYS
                     FPLNNLVCSYDPVKDVLLPDYGLSNLTACNWCHGSSVRRVRGDGSIVYLDGDRTNSRS
                     TGGKCGCGFKHFWEGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDPSLNSNVYDV
                     AMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQE
                     LESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKG
                     QPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKPSTTFFE
                     LQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKGRAEG
                     GPSTAAHSAHTVKQEEIAVTGKLSSKELQEQADKEMYSLCLLATLMGEDVWSYAKGLP
                     HMFQQGGVFYNIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGP
                     DVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHS
                     LGTASSHPHIDPRARETLAVRKHNTGTDFSNSSIKTEPPVFTAASSNSELIRIAPGVV
                     TMRDGRQIDPDVVEAQRKKLQEMVSSIQASMDKHLRDQSAEQAPSDLSQRKVEVVSSV
                     RPVNLQTGLPEPFSLTGGTENLNTETTDSHVADVLGAAFATRSKAQKENSMEEPEEMD
                     SQDAETTNTTEPMDHS"
     exon            2934..3020
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..9749
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5154..5159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATACA"
     polyA_site      5179
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="major polyA site"
     polyA_site      5201
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      7940..7945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      7960
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      8416..8421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      8438
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      8864..8869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AGTAAA"
     polyA_site      8886
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      9609..9614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     regulatory      9618..9623
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: GATAAA"
     polyA_site      9630
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     polyA_site      9635
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      9723..9728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: ATTAAA"
     polyA_site      9743
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
ORIGIN      
        1 tgcggggtgg ggtggtggcc gttacgttcg gggcaacggc taaggcagtg gagaagtgag
       61 aagagaaaca acgtccggcg cttccgcctt cgcctaggag gaggaggaac tggtaggaaa
      121 aggaaagcca ttcgccctgg gcctggacta gacctcgtcg ggccggcgga ggtctggcta
      181 tgtgcctttg agggtcgctt gggacgccga gcgagagccg agagcgatgt ctcagccgcc
      241 gccgcctcct ccgctgccgc cgccgccgcc tccccctgag gctccgcaga cttcgtcgtc
      301 tctggcggcg gcggcttctc cggggggcct ttcgaaacga agggaccgaa gaatcctttc
      361 cgggagttgc ccggatccca agtgccaggc gcgcctcttc ttcccggcct cgggttctgt
      421 cagcatcgag tgtaccgagt gcggacagcg gcacgagcag caacagctgc tgggagtgga
      481 ggaggtgacc gacccggacg tagtgctgca caatctgctg cggaacgcgc tactcggagt
      541 gacgggggct cccaagaaga acacggaact ggtaaaggtg atgggcctct ccaactacca
      601 ctgcaaatta ttgtcgccaa tattggcacg ctatggaatg gacaaacaaa cgggccgggc
      661 caagcttctc cgggacatga accaaggcga actgttcgat tgcgcgttac ttggtgaccg
      721 ggccttcctc atcgaaccag agcacgtcaa cacagtgggc tatggcaagg accgctccgg
      781 aagcctcctg tatttgcacg acacgctcga ggatatcaaa cgggctaata aaagtcaaga
      841 atgcctcatc ccagtgcatg tggatgggga tggacactgc ctagtgcatg ctgtgtctag
      901 ggctctagta ggccgagagc tcttctggca tgctctgagg gagaatctta agcagcactt
      961 tcagcagcac ctagcccgat atcaagccct gtttcatgac tttattgatg ctgccgagtg
     1021 ggaggacatt atcaatgaat gtgaccctct gtttgtgcca cctgaaggtg tccccttagg
     1081 cctgaggaat atccacatat tcggccttgc caatgtgtta catcgtccca taattctttt
     1141 agattccctc agcggcatga gaagctctgg tgattattca gccacctttc tacctggcct
     1201 catacctgca gagaaatgca ctgggagaga tggtcatttg aacaagccaa tctgtattgc
     1261 gtggagtagc tctggtagaa accattatat tcccttggta ggcataaagg gggctgccct
     1321 gcccaaacta cccatgaatt tgttacctaa agcatggggt gtgcctcagg accttattaa
     1381 aaagtacatc aagcttgagg aggatggtgg ctgtgttatt ggaggagaca gaagtttgca
     1441 ggataagtac ttacttaggc tggttgctgc tatggaagaa gtctttatgg acaaacatgg
     1501 tatccatcct agtttggttg ctgatgtaca tcagtatttc tacagaagga ctggggtgat
     1561 aggagttcag cctgaggaag ttacagcagc tgctaaaaag gcagtaatgg acaatcgcct
     1621 tcacaagtgt ttgctttgtg gtgccctttc tgaacttcat gtcccttctg agtggttggc
     1681 tccaggaggg aaactgtata acttggcaaa aagtactcat gggcagttga ggcctgacaa
     1741 aaattacagc tttcccttaa acaatttggt ttgttcatat gatccagtaa aagatgttct
     1801 gctaccagac tatggattga gtaatctaac agcttgtaat tggtgccatg gctcatctgt
     1861 gcgaagagtc agaggagatg gctctattgt gtatttggat ggagacagaa ctaattctag
     1921 gtctactggt ggcaaatgtg gttgtggatt caaacatttt tgggaaggta aagagtatga
     1981 caatcttcca gaagcttttc ctatcacttt ggagtgggga ggaagagtag tcagagaaac
     2041 agtatattgg tttcagtatg aaagcgatcc atctttgaat agtaatgttt atgatgttgc
     2101 aatgaaactt gttaccaagc actttccagg tgaatttggg agtgagatcc tagttcagaa
     2161 agttgtccac actatattgc atcagactgc aaaaaaaaat ccggacgatt acactcctgt
     2221 aaatatagat ggtgctcatg ctcagagagt tggagatgtg caaggacaag aattggagtc
     2281 gcagctacca actaaaatta ttcttactgg acagaaaaca aaaactttgc ataaggaaga
     2341 attaaacatg agtaaaactg aaagaactat tcaacagaac atcacagaac aagcttctgt
     2401 gatgcagaaa cggaaaacag agaaattaaa acaagagcaa aaggggcagc ccagaactgt
     2461 ttctccaagt actattcgtg atgggccatc atctgcacct gccaccccca ccaaggctcc
     2521 ctactcacct accacttcta aggagaagaa gattcgaata acaactaacg atggacggca
     2581 gtctatggtt acccttaagc cttcaacaac cttttttgag cttcaggaaa gtatagccag
     2641 agagttcaac attcctccat atttacagtg tattcgatat ggctttcctc ctaaagagtt
     2701 aatgccaccc caggcaggga tggaaaagga gccagttcct ttacagcacg gtgacagaat
     2761 tacaatagag atcttaaaag gaagagcaga aggtggtcca tccactgctg cacactcagc
     2821 ccacactgtg aaacaagaag agattgctgt tactggtaaa ctgtcatcta aggaacttca
     2881 ggagcaagct gacaaagaaa tgtactcctt gtgtctttta gctacattaa tgggagaaga
     2941 cgtgtggtct tatgcaaagg gacttcctca catgttccag cagggtggtg tattctacaa
     3001 tattatgaag aaaactatgg gcatggctga tggcaaacat tgtacttttc cacatctacc
     3061 tggcaaaacc tttgtttata atgcttctga agatagactg gagttgtgtg tcgatgctgc
     3121 aggacatttc cccattggtc ctgatgttga agatttagtt aaagaggctg taagtcaggt
     3181 gcgagcagag gctactacaa gaagtaggga atcaagccct tcacatgggt tattaaaact
     3241 aggtagtggt ggagtagtga aaaagaaatc tgagcaactt cacaatgtaa ctgcctttca
     3301 ggggaagggc cattctctag gaactgcatc tagtcacccg cacattgatc ccagagctag
     3361 ggaaactctg gctgtgagaa agcataatac agggacagat tttagtaata gttccattaa
     3421 aacagagcct cccgtgttca cagctgcttc tagtaatagt gagcttattc gaatagctcc
     3481 tggagtagta acaatgagag acggtaggca gattgatcct gatgtggttg aggcccagcg
     3541 gaaaaaattg caggaaatgg tttcttctat tcaggcttca atggacaagc acttgcggga
     3601 tcaaagtgca gagcaagcac catctgatct ttcccaaaga aaagtcgaag ttgtgagttc
     3661 tgtgaggcct gtgaatcttc agactggctt gcctgaaccg ttttctttaa ctggtggcac
     3721 tgagaatttg aatactgaaa caactgatag tcatgtagca gatgtactgg gagcagcatt
     3781 tgccacaagg tcaaaagcac aaaaagaaaa ttccatggag gaacctgaag agatggatag
     3841 tcaagatgct gagacgacta acacaactga gccgatggat cactcgtgat ttaatttaga
     3901 ggctaataaa ggcagaatgt ttattgtgaa tatgtaatat ttgttggctg ggccacataa
     3961 cttgagtagt cattaaaaat cttgtacgta tataattcaa agattatatc ttgttattca
     4021 gtgcatgata gcaagtgtgt gattggcaat agcttttaat atactgctgc ccaggctggc
     4081 tctgaattct tgtaaattag tttagatctt ttgaaagggt ttcttccaga tatgtggttc
     4141 attggaagtt ctttgtcatt tttaattcca tgaaagtctt aatttccaga catgaaaatt
     4201 ctcttgttaa atgtttggtt tctgcacagg ccagaattga agaggaaatg acagtttgta
     4261 cacattgttt tcaaggtagc cgtcctacat ttcatgagaa tgttgatttc aagtttacct
     4321 gaactaaatg tatcttaaaa tacataaaac actttattag attacttata atattataga
     4381 gacagctatt gttggcaaaa atatcttgac agaagagagt agtgatacaa cattttcctg
     4441 atttgagcaa acttgggaag aaatatctac cctagtatga taaaatcagt gtgattcagt
     4501 tagttggtct gagatatggc cagttgtgtg ctcttgttga taatatgttg ccagtatttt
     4561 ttaaatcttg gccttgtcaa gttccaccta ttatttttaa aatcttacat aaaagagaat
     4621 ggcattttgt tttagaatat ttaaaaataa tggtagcagt agttaatttc taattttcag
     4681 agtatgtcag agtcacaaag agtttatctg tatgaatggt tctatagttt tgacacagtg
     4741 gtgtgtaagc ctgtatatgc taaatgtctc taagccccta tgaaccaaaa atgttttcct
     4801 tttttcttag tgtccttaag attttctttt tgtcagcttc acttgagttt ggatgcatag
     4861 tttactccag tagaccatta gcatttgctt atattgcttt catgcttatg tagtaaaaac
     4921 aatgcatatt cctgagattg gtactgctct tgtattctgt tctgtagatt tgtatttgta
     4981 aatgtgtttt cagtccatct tcctttgttt actgaaatta agttgaattt gaatgaacga
     5041 atgacaaatg tatgttaata taatctctta gagcattcaa agcccggcta tctctgtttg
     5101 cacatgtaac agatgcagaa ctgtttgcaa ttctacaaaa ttgtatgtca gaaaatacat
     5161 tatcaggtgt ttaaaaaaat ctcttcctgt tcattcgaaa agaatgttgt acttgaactt
     5221 tttctagata agggaggttt tttgtttttt tctgtgatag tatctttaag tgagtctaca
     5281 tactgaaagt ccactttgtt gaatgtggaa ggaagggaga atctagatgt ttttaaagtg
     5341 gagctgagtt taagataatg tacaccacca cacacacctg ggttagtgtt tatcacatca
     5401 tactttgaaa tattttatct cattcattta tattaaagtt ccaggaaaat atataacttt
     5461 catatactaa ttttgcatga agtaagatag ccaaattctt aacaattagc tgtgctttaa
     5521 tgccccccca acccctccac taaccactat tattttggct tagtcagcca ctgagatact
     5581 cttctcaatt ttttttcctg gtttaagtac acaaaaactg ctcaataagc agaaaatgta
     5641 ctgtgtcaaa taaattatat aatcaccctg ctttaacaga tgctcttctg tggcttggta
     5701 ttttaagtta ataactggca ggatctcaaa gtaggagtat cagaaacaag ttttgttttt
     5761 tccagacatg gtcttactac tatgtagcct tggctggcct ggagcccact ctatagacca
     5821 ggctggcctt aaaaagagat ctgcctgcct ccgtctccca agtgttggga ctagaaggca
     5881 tactatacca tgcctggcta gagactaatt ttatctctct attaaatact tgaatttcgt
     5941 gtaaattatg cacatgtgaa tgttagaaga tggacttaat ctctgacaat acttcattgt
     6001 ctctagtgac cgtagtggta tttagggtta gaaacttaat tgggtataaa gaaaagtttg
     6061 ggttctccct tgatagaaaa tacagacaaa caaccaaaca aaaaacaggt ttgagaattc
     6121 atcagaattc ttgaacatat ttcaaatcag ttgaatgaga ttattagtgg tttggggtaa
     6181 attttgagag agtttgtttt aaacattatt tgtgtatttt atgtgagtgt agatgcattt
     6241 ttaccattgt gataccactg tgctatgtca gggcagcaca atagcttgtg atctcaagta
     6301 aaaatggttt tttagcccta gaagtaacat aactgtaact taaagctatt cagttctatt
     6361 tgcagtgttc actgaatact gaagtaatac tgtaaatatt tttcttcagt aatccatttt
     6421 gtaaccatac caagtttgag ttgatgaccc gaagtccagg cagttaaagg tagtgtatgt
     6481 ccagaaggct ggtaatgaac aattgctcaa gttgaaggaa acaagacact gccaagtatt
     6541 atgagtagta tacaattagc tccaagggaa tggtcactca gaaactaaag aacaagccta
     6601 tctagccatt tcatcttcca ccccttaaac tatgtttcag gtgtttattg gaaataatta
     6661 ggaaagagac ctgagaaatt tagcaaatgc tagtatttga aatgctttat accttcctat
     6721 agtctttagt ttggttttta caattaagct ttactgcttg cagttgagat agcattctgg
     6781 aatgtacttg tgagactagc cacaggtctt acagatcttt attgccatta ttttaaggat
     6841 catattcatt ggatattaac actgtcttaa atagggtctc ttacctcagg gaagataatt
     6901 tgctagcaac tcctatatta ttattgacag gttttggaga actactttga catatcacat
     6961 gttagctacc aaatgcaaaa acaagtatta tgtagttacc tgctgtctgc tactgaacat
     7021 ttctctagat atttagggcc ctattattaa gtaacattta aatacgaaat aaacttctaa
     7081 gttctgtcat tagatacttc atgttttcac tatagtgata ctatattttt ttaaatttca
     7141 gaacattaat atagttttgt cctcattttt caagtcaatt taatttgccc aaacttaaat
     7201 actcaaatac aaacaaatct tgagaatttt atttttcttg cttcccctcc caccaccctt
     7261 gatctcaaag caagattttt ataagattct tataaaatca gagcccttgc aaaattggtt
     7321 taaattcctc tcattttaaa cttagattca aagttacttt tttataacat aaatagatgc
     7381 atattctagg ttggctagat gttcaacttt ccaagactat aacaaaattg tacaacatag
     7441 aggctcttca cagttagatt tttttttttt ttcaaacaag aacagctcat gaaaataggc
     7501 catggtagta aaatgtgtat ttaagtaacg aaggggagat aaatcactaa tgtcacagag
     7561 ttaattgcaa gttctgccat tctttgagat gccaagtgtt tcaattcata tttaatgata
     7621 ctcaaatcgt ggaaatttta acgtttgttc caaagtagca gaaactgtaa aaaaaaaaaa
     7681 aattgtgaat tattgaagaa aataccatta cttgtatacc tgacatttaa agtttttaat
     7741 tatggagttg acatgttttt gtagtataat aatacaatga ctattctctt gtaaaagaaa
     7801 aggaaaaact agtaaagaaa aagttttagt tgatgttatt tgcttgtatc aattactagt
     7861 attttaaaat ttaaggtatt gcatacttta cgctagaatg cagaaatccc tcctggagcc
     7921 tttatgcctg ttgtagagca ataaaggact gaaaagctta tcaatatttt caggtttgtt
     7981 tgactgagta ttagtgctaa gaattgaaga gtctcctttt ttggagggaa atgctactta
     8041 gattaaaaca aagccataat agtataaata tttagacaaa aaactaacaa gtactttaac
     8101 ttggcagtga agtagtatag atgctattat ctgtaaggca caagctgtta gacattactt
     8161 tctggtttca tggttgttta gtatatatta aaattgagaa taaataattg gtagcagtat
     8221 aacagtatag tgtttttgag cacacatcac tgtacagtat ttgaaatttt gtattaatag
     8281 aaaactaaat aatatattcc ccctcaactt cacattggct ttttaaaaag taatgttctt
     8341 tagtttcctc taattagagt ttaataccat aatgcatgaa caaatagttc agttttataa
     8401 taacattttt agcataataa agttgttttt aaaacttatg gccaggcagt ggtggcacac
     8461 gccttttatt ccagcacttg ggaggcagag acgtgcggat ttcttagttc gagaccagcc
     8521 tcgtctacag agtgagtacc aggacagcca gggctgtaca gagaaaccct gtctcgaaaa
     8581 aggaggaaaa aaatcaaaaa caaacaaaaa cgaaaaacaa caacaaaaaa atgcccatac
     8641 atatgaatta cccaggaact gtaatttctt tccttaatgc aggttttaat attagatgaa
     8701 atgaatccaa ttataaaaaa aaatatgcaa aatttctcta gaaattttgg gtcatgtaat
     8761 gcttactaaa tatttcaaaa tgagatatgt tctaattgag aatttatttt tgaaaagagt
     8821 acttgttatg atgaggatat gaagggaaaa tatagaaaac agcagtaaag tcctcatagc
     8881 tcaccactca actgtccact ttgatttatt tttaagcctt agtttattta tttaatatct
     8941 attggatcac agtattggat cagtggttag aactgcccta gactccagac tcgggtccaa
     9001 gtcaagcata caacctactg tgtaacctct gtatcactcc caagaaggat gataatggca
     9061 tttatcaata gagctgggag gattgatgag ttattacctg aacattaaaa ccatgtctgg
     9121 tacagtgtac cttcaaatac tttctgtggt tagtatgctt ttaagttgtg gtaagagttc
     9181 acctgccact gacatgcagt acagccttac atgtaggcca tgtgtgttat actcttaaat
     9241 ctaaaacttg ttgaatgtgt atagtatgtt accaacgagt agaaactgga tatttttaca
     9301 tccaaatatt tctatgaggg cttaatctca cgaactacca agaacctgga gagttggcct
     9361 tctgtgcttt gtgattgaga tgtgctatgg agcctttaaa ggcccataaa gctgctgtaa
     9421 ccttgaggtt tgcagttgta cctctgttct gttgaccata ggtacaatgc tgagagtagt
     9481 gctgaagcaa caggcactgt gaactcagaa tgctgagact acagtcacaa acttgagctt
     9541 gaggtcttct gtgtgtgtgc atgccattgt ccatatacca tcaagataga gctctcaaaa
     9601 cttcagtcaa taaagaagat aaacattcca cttaagatag ttcattactg gttttagttt
     9661 tattgactgg ctgtcatacc ccagtaagta aaataaggct gaataattga ttgtttctaa
     9721 taattaaaaa tgtacagaga aaagttgaa
//
