LOCUS       NP_033525                375 aa            linear   ROD 05-JUN-2024
DEFINITION  vasodilator-stimulated phosphoprotein isoform 1 [Mus musculus].
ACCESSION   NP_033525
VERSION     NP_033525.2
DBSOURCE    REFSEQ: accession NM_009499.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 375)
  AUTHORS   Waldman,M.M., Rahkola,J.T., Sigler,A.L., Chung,J.W.,
            Willett,B.A.S., Kedl,R.M., Friedman,R.S. and Jacobelli,J.
  TITLE     Ena/VASP Protein-Mediated Actin Polymerization Contributes to Naive
            CD8+ T Cell Activation and Expansion by Promoting T Cell-APC
            Interactions In Vivo
  JOURNAL   Front Immunol 13, 856977 (2022)
   PUBMED   35757762
  REMARK    GeneRIF: Ena/VASP Protein-Mediated Actin Polymerization Contributes
            to Naive CD8(+) T Cell Activation and Expansion by Promoting T
            Cell-APC Interactions In Vivo.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 375)
  AUTHORS   Dennison,B.J.C., Larson,E.D., Fu,R., Mo,J. and Fantauzzo,K.A.
  TITLE     Srsf3 mediates alternative RNA splicing downstream of PDGFRalpha
            signaling in the facial mesenchyme
  JOURNAL   Development 148 (14) (2021)
   PUBMED   34184034
REFERENCE   3  (residues 1 to 375)
  AUTHORS   Ali,M., Zuzga,D.S. and Pitari,G.M.
  TITLE     Differential Ser phosphorylation of vasodilator-stimulated
            phosphoprotein regulates colon tumor formation and growth
  JOURNAL   Life Sci 264, 118671 (2021)
   PUBMED   33129878
  REMARK    GeneRIF: Differential Ser phosphorylation of vasodilator-stimulated
            phosphoprotein regulates colon tumor formation and growth.
REFERENCE   4  (residues 1 to 375)
  AUTHORS   Cheng,K.W. and Mullins,R.D.
  TITLE     Initiation and disassembly of filopodia tip complexes containing
            VASP and lamellipodin
  JOURNAL   Mol Biol Cell 31 (18), 2021-2034 (2020)
   PUBMED   32579429
  REMARK    GeneRIF: Initiation and disassembly of filopodia tip complexes
            containing VASP and lamellipodin.
REFERENCE   5  (residues 1 to 375)
  AUTHORS   Iwasaki,K., Obashi,K. and Okabe,S.
  TITLE     Vasodilator-stimulated phosphoprotein (VASP) is recruited into
            dendritic spines via G-actin-dependent mechanism and contributes to
            spine enlargement and stabilization
  JOURNAL   Eur J Neurosci 51 (3), 806-821 (2020)
   PUBMED   31793065
  REMARK    GeneRIF: Vasodilator-stimulated phosphoprotein (VASP) is recruited
            into dendritic spines via G-actin-dependent mechanism and
            contributes to spine enlargement and stabilization.
REFERENCE   6  (residues 1 to 375)
  AUTHORS   Hauser,W., Knobeloch,K.P., Eigenthaler,M., Gambaryan,S., Krenn,V.,
            Geiger,J., Glazova,M., Rohde,E., Horak,I., Walter,U. and Zimmer,M.
  TITLE     Megakaryocyte hyperplasia and enhanced agonist-induced platelet
            activation in vasodilator-stimulated phosphoprotein knockout mice
  JOURNAL   Proc Natl Acad Sci U S A 96 (14), 8120-8125 (1999)
   PUBMED   10393958
REFERENCE   7  (residues 1 to 375)
  AUTHORS   Collins,S.P. and Uhler,M.D.
  TITLE     Cyclic AMP- and cyclic GMP-dependent protein kinases differ in
            their regulation of cyclic AMP response element-dependent gene
            transcription
  JOURNAL   J Biol Chem 274 (13), 8391-8404 (1999)
   PUBMED   10085070
REFERENCE   8  (residues 1 to 375)
  AUTHORS   Lanier,L.M., Gates,M.A., Witke,W., Menzies,A.S., Wehman,A.M.,
            Macklis,J.D., Kwiatkowski,D., Soriano,P. and Gertler,F.B.
  TITLE     Mena is required for neurulation and commissure formation
  JOURNAL   Neuron 22 (2), 313-325 (1999)
   PUBMED   10069337
REFERENCE   9  (residues 1 to 375)
  AUTHORS   Aszodi,A., Pfeifer,A., Ahmad,M., Glauner,M., Zhou,X.H., Ny,L.,
            Andersson,K.E., Kehrel,B., Offermanns,S. and Fassler,R.
  TITLE     The vasodilator-stimulated phosphoprotein (VASP) is involved in
            cGMP- and cAMP-mediated inhibition of agonist-induced platelet
            aggregation, but is dispensable for smooth muscle function
  JOURNAL   EMBO J 18 (1), 37-48 (1999)
   PUBMED   9878048
REFERENCE   10 (residues 1 to 375)
  AUTHORS   Zimmer,M., Fink,T., Fischer,L., Hauser,W., Scherer,K., Lichter,P.
            and Walter,U.
  TITLE     Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes
            in human and mouse: structure, sequence, and chromosomal
            localization
  JOURNAL   Genomics 36 (2), 227-233 (1996)
   PUBMED   8812448
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB850928.1, AK140329.1 and CO040918.1.
            
            On Nov 20, 2007 this sequence version replaced NP_033525.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010223.1, AK140329.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849389, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.51 cM"
     Protein         1..375
                     /product="vasodilator-stimulated phosphoprotein isoform 1"
                     /calculated_mol_wt=39536
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Region          5..113
                     /region_name="EVH1_Ena_VASP-like"
                     /note="Enabled/VASP family EVH1 domain; cd01207"
                     /db_xref="CDD:269918"
     Site            order(16,23,71,73,79,81,83)
                     /site_type="other"
                     /note="proline-rich peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269918"
     Site            39
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            46
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Region          111..340
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Site            153
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by PKA, PKC, PKG/PRKG1 and ROCK1.
                     /evidence=ECO:0000269|PubMed:10882740; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Region          215..241
                     /region_name="WH2_hVASP-like"
                     /note="Wiskott-Aldrich syndrome protein (WASP)-homology
                     domain 2 (WH2) of human Vasodilator-stimulated
                     phosphoprotein and related proteins; cd22185"
                     /db_xref="CDD:409225"
     Region          221..373
                     /region_name="EVH2"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Region          221..241
                     /region_name="EVH2 block A"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Site            order(222..223,225..226,229,231..235)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409225"
     Region          230..233
                     /region_name="KLKR"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Region          231..234
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409225"
     Site            235
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by PKA and PKG.
                     /evidence=ECO:0000269|PubMed:10882740,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Region          245..>360
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     Region          257..274
                     /region_name="EVH2 block B"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Site            274
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by PKA, PKG/PRKG1 and AMPK.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            279
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            309
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            311
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P50552; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            317
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            318
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by AMPK.
                     /evidence=ECO:0000269|PubMed:21945940; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Site            320
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (P70460.4)"
     Region          337..371
                     /region_name="VASP_tetra"
                     /note="VASP tetramerization domain; pfam08776"
                     /db_xref="CDD:462599"
     Region          338..372
                     /region_name="EVH2 block C"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     Region          339..368
                     /region_name="2 X 15 AA tandem repeats of L-[EQ]-[KR]
                     [MV]-K-[EQ]-E-[IL]-[IL]-E-[AEV]-[FV]-[KRV]-[KQ]-E"
                     /note="propagated from UniProtKB/Swiss-Prot (P70460.4)"
     CDS             1..375
                     /gene="Vasp"
                     /coded_by="NM_009499.3:337..1464"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS52056.1"
                     /db_xref="GeneID:22323"
                     /db_xref="MGI:MGI:109268"
ORIGIN      
        1 msetvicssr atvmlyddsn krwlpagtgp qafsrvqiyh nptansfrvv grkmqpdqqv
       61 vincaiirgv kynqatpifh qwrdarqvwg lnfgskedai qfatgmanal ealegggppp
      121 apappawsaq ngpspeeleq qkrqpehmer rvsnaggppa ppaggppppp gpppppgppp
      181 ppglpssgvs gaghgagaap ppapplptaq gpnsggsgap glaaaiagak lrkvskqeea
      241 sggplapkae nsrstggglm eemnamlarr rkatqvgekp pkdesasees earlpaqsep
      301 vrrpweknst tlprmkssss vttseahpst pcssddsdle rvkqelleev rkelqkmkee
      361 iievfvqelr krgsp
//
