LOCUS       NM_001356527            5253 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus vesicle-associated membrane protein 4 (Vamp4),
            transcript variant 4, mRNA.
ACCESSION   NM_001356527 XM_006496934
VERSION     NM_001356527.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Hara,Y., Katsuyama,T., Fukaya,M., Sugawara,T., Shiroshima,T.,
            Sadakata,T., Osumi,N. and Sakagami,H.
  TITLE     ADP Ribosylation Factor 4 (Arf4) Regulates Radial Migration through
            N-Cadherin Trafficking during Cerebral Cortical Development
  JOURNAL   eNeuro 10 (11) (2023)
   PUBMED   37848288
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5253)
  AUTHORS   Li,M., Feng,F., Feng,H., Hu,P., Xue,Y., Xu,T. and Song,E.
  TITLE     VAMP4 regulates insulin levels by targeting secretory granules to
            lysosomes
  JOURNAL   J Cell Biol 221 (10) (2022)
   PUBMED   36053215
REFERENCE   4  (bases 1 to 5253)
  AUTHORS   Ivanova,D., Dobson,K.L., Gajbhiye,A., Davenport,E.C., Hacker,D.,
            Ultanir,S.K., Trost,M. and Cousin,M.A.
  TITLE     Control of synaptic vesicle release probability via VAMP4 targeting
            to endolysosomes
  JOURNAL   Sci Adv 7 (18) (2021)
   PUBMED   33931449
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5253)
  AUTHORS   Liu,Y., Sugiura,Y., Sudhof,T.C. and Lin,W.
  TITLE     Ablation of All Synaptobrevin vSNAREs Blocks Evoked But Not
            Spontaneous Neurotransmitter Release at Neuromuscular Synapses
  JOURNAL   J Neurosci 39 (31), 6049-6066 (2019)
   PUBMED   31160536
REFERENCE   6  (bases 1 to 5253)
  AUTHORS   Williams,D. and Pessin,J.E.
  TITLE     Mapping of R-SNARE function at distinct intracellular GLUT4
            trafficking steps in adipocytes
  JOURNAL   J Cell Biol 180 (2), 375-387 (2008)
   PUBMED   18227281
REFERENCE   7  (bases 1 to 5253)
  AUTHORS   Sadakata,T., Itakura,M., Kozaki,S., Sekine,Y., Takahashi,M. and
            Furuichi,T.
  TITLE     Differential distributions of the Ca2+ -dependent activator protein
            for secretion family proteins (CAPS2 and CAPS1) in the mouse brain
  JOURNAL   J Comp Neurol 495 (6), 735-753 (2006)
   PUBMED   16506193
REFERENCE   8  (bases 1 to 5253)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   9  (bases 1 to 5253)
  AUTHORS   Zeng,Q., Tran,T.T., Tan,H.X. and Hong,W.
  TITLE     The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their
            correct subcellular targeting: the N-terminal extenSion of VAMP4
            contains a dominant autonomous targeting signal for the trans-Golgi
            network
  JOURNAL   J Biol Chem 278 (25), 23046-23054 (2003)
   PUBMED   12682051
  REMARK    GeneRIF: The cytoplasmic domain of this protein is responsible for
            its correct subcellular targeting, the N-terminal extension
            contains a dominant autonomous targeting signal for the trans-golgi
            network
REFERENCE   10 (bases 1 to 5253)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138218.15.
            
            On Oct 8, 2017 this sequence version replaced XM_006496934.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.179444.1,
                                           SRR9219382.116868.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-266               AC138218.15        117416-117681
            267-385             AC138218.15        121199-121317
            386-432             AC138218.15        124773-124819
            433-483             AC138218.15        128720-128770
            484-584             AC138218.15        135097-135197
            585-664             AC138218.15        136366-136445
            665-716             AC138218.15        139527-139578
            717-5253            AC138218.15        141447-145983
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 70.29 cM"
     gene            1..5253
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="vesicle-associated membrane protein 4"
                     /db_xref="GeneID:53330"
                     /db_xref="MGI:MGI:1858730"
     exon            1..266
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            267..385
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..745
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="isoform 2 is encoded by transcript variant 4"
                     /codon_start=1
                     /product="vesicle-associated membrane protein 4 isoform 2"
                     /protein_id="NP_001343456.1"
                     /db_xref="CCDS:CCDS83616.1"
                     /db_xref="GeneID:53330"
                     /db_xref="MGI:MGI:1858730"
                     /translation="MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGP
                     RFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRS
                     KQLRRQMWWRGCKIKAIMALAAAILLLMIIILIVVKFRT"
     exon            386..432
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            433..483
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            484..584
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            585..664
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            665..716
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     exon            717..5253
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1303..1308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="hexamer: AGTAAA"
     polyA_site      1331
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="major polyA site"
     regulatory      1420..1425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="hexamer: AATAAA"
     polyA_site      1442
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="major polyA site"
     regulatory      1511..1516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="hexamer: AATAAA"
     polyA_site      1541
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
     regulatory      5227..5232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="hexamer: AATAAA"
     polyA_site      5253
                     /gene="Vamp4"
                     /gene_synonym="D1Ertd147e; Vamp-4"
                     /note="major polyA site"
ORIGIN      
        1 gagcctgccc ccttgcgccc tctccaggac tcggagtacc gagcggctcg atctccctcg
       61 cctgagcccg ccccctctgc ccagcgccag gcggcggagc gccgagtcgc tcgatgagcg
      121 ggggcgctgc agcagagcgg agcggtggcg gctctgcgcc ttcggccttc cccctcccac
      181 ccttcttcct ccgcttcccg ctccctcccc cgcctgccct gcgctgccag cagggactgc
      241 tgccgggagc ccatcggaga ccgcaggtgg tgaattctta ctctgttggg aaacacagga
      301 acatttgtga gcaggaagta tgcctcccaa gttcaagcgc cacctaaatg atgatgacgt
      361 gaccgggtct gtgaaaagtg agaggagaaa tcttttggaa gatgattcag atgaagaaga
      421 ggactttttt ctacggggac catctggacc aagatttgga cctagaaatg ataaaattaa
      481 gcatgttcag aatcaggtgg atgaagttat tgatgtaatg caagagaata ttacaaaggt
      541 aattgaaaga ggagagaggc tggatgaact acaggacaaa tcagaaagct tatcggataa
      601 tgccaccgct ttcagcaaca gatccaaaca gcttcgaagg caaatgtggt ggcgtggatg
      661 caaaataaaa gccatcatgg ctttggctgc agcgatcctt ttgctaatga ttatcattct
      721 tatagttgtg aaattccgta cttgatttga tgacagagac cctcattaaa caagatctgg
      781 gacaataata tttaaagatt gctgcataat gtaaatgaaa cctatgtata gaactttcaa
      841 atttcctttt tcaagaaact aaaaggcaag taccaattca aattggagca ttgagaatat
      901 cgaatgatca cctttccaac taacaaaatg cattttgaat aattatattt gatgtagaaa
      961 tatctctatt ttgctttatt ccaaggatct aatttgaaac tagatacttg ccagtctgtt
     1021 atttgtatat atagttgaac cctgataaca atgccccata cttgtgtttc agttgcatta
     1081 aagacattat tgcatctaag tgagagtcag tctggtgacc aggaagccta tgtagagtcc
     1141 tgactctttg ctgcaagaga tgcataacca tcctatcagt atttgcaaga atgccattct
     1201 tttcatattg tagttgcttt gcaggcaatt tccacactta tggattaaca aaagctaagt
     1261 gttgtaaatg tttctgcctt cagctctacc cgaaaacatt ccagtaaacc tattctgact
     1321 gtataagaaa atacgtggta attttttggt ctttggattt tgaaaatggg aaatttaaac
     1381 agtgtgaaaa tcataatatg gtcctataaa actggagata ataaaatttt tgaagaatgt
     1441 cattggagtg tttgaaaacg agcactatgt agctcccagt cttggtccag ttggttgtca
     1501 gttaaactac aataaaaagg gactaattct gacgtgagta agtgaaggct gcaggtaatt
     1561 tttttcttga cttcattttt ttagtggttt tctcttttta attttaacta accctgtgcc
     1621 agaggtgctg aagttagcgc agtcatgtgc ctctcacctt gtattctcat gctctctgac
     1681 actcctgagg agatgggtgt cacaggtctt caaacagcca gtgtcagtca gtagccgact
     1741 gtcaggagca tagatgtctt cacatactca ctcagtgact gatgctgagg ggccaccatt
     1801 gaccccagcc tgctcttagg acaagttgtg ttccttgtaa agagtactgg cctcttaata
     1861 ttgattaaat aagtcaaagt gaaggtgctt cctgctgtgc agggggaagg cagagaccac
     1921 aggaactgtt cacatggaca tctcaagctg gaaagagagc tcacaggggc ggccaggatt
     1981 gggggcaatg gtcctgtata acagatatta gtttttgtga tcattttaat tcaaaatgtg
     2041 taaaacaatt tggatttttc tttgtttaat tctacaatgg ttaatactaa cagacaaaat
     2101 gtttagactg gaaagaaact tgaaaaggtg gaaacaagat taagtggtag ccgagccggg
     2161 catggtggca cacaccttta atcccagcac tcgggaggca gaggcagaca cagacggatt
     2221 tctgagttcg aggccagcct ggtctacaaa gtgagttcca ggacagccac ggctacacag
     2281 agaaaccctg tctcgaaaaa ccaaaaaaaa aaaaaaaaaa aaaaaagatt aagtggtagc
     2341 ctaggtatct aagtataatt gctggagttt acagtttgtt gcacactgtg gaaaatcgac
     2401 tctggccttt tcaccatgct cgtcaattcc tgtgcgcagc atctgaactg agaatacatc
     2461 atttggagaa ggagtgctct gcattccagc agctggtggc tcagggatgg aggtgtttcc
     2521 tccatcagca cagctgatcc cagtgtgtgt gtcttgtacc tcaggtcaca agttccactg
     2581 cttggtgtgg aagacactgt cactcagtgt ctgttagcga agatgaagtg ttgggtgttt
     2641 ttctttagaa gactccaaat ttcaatcttc ttttaattaa gtatgccact tttaaacaaa
     2701 caattataaa acatagaatt gtaaagttaa ttttaaaaca ttaattataa aacatattac
     2761 ttaactacct atatatgtag ttgtttatat ttaaatttct cgaatctgag gaaatcctat
     2821 tgtcatggga ctgaggattt tacaacagtg atggcttcaa tcccagttta tcactttgtc
     2881 ccttttggtc ttatgtaatc ctgagaccta acaatgacat tagggtgaaa tactggaaat
     2941 gtgggtgttt ctacaataag atgctgagaa gagccaggtg tgatggctca cacctgtaac
     3001 ccctgctcta tgaacactga agtaggaagg tttctctgat tttgaagcca tcctggggtt
     3061 catggtgtgt tctgtaccag cctgggctat agagtaagaa cctgtctggg ggaagaaaaa
     3121 aaaaagccaa ccaagcaaac aaatcagagg agaaacagtt gtcagactga tgtcaggcct
     3181 gccttccttc cctccttaat cattcttttt ctcattcagc tcagatcatc ctgcacttga
     3241 aaaaatgaag ttttaccacc atcaaaacta tcactattta acaacagtga agatcaacag
     3301 aaaaattcaa gtagctttgc taatgttagt tgatgcccag tgaaatcagg tcaccactgc
     3361 cgtgcctgat gcgtgcccct gtggtttttc tgtaacagaa gcctctccct tgcagcagtt
     3421 tgtgatgaat gggttcctcc tccatcataa cccagtgtta gtgttgcctg tggtcttttt
     3481 attcctttgt cctaacaaaa ctcattggga aatgtcccaa aagtttataa gctgggaaat
     3541 ttattgatta agagaaggtc cagtgctgat gatggaacat tgtttaaaat ggagaaaggg
     3601 tagcacacat cagttgcaga ggtgcctgtg atggggttcc tgtagactct cattacagtc
     3661 cactctgggt tctgtgttag aaagtaatgc cattctgata gcagcaaccg gcacctttct
     3721 gttctgtatg gcatgtgggt gccctcagat gtgataagag cctgacctct gtccttgtca
     3781 cttaacacct gttctgtgtg ctgtcggcac tgtagaccaa acagacttgt atttacatct
     3841 catagatatt ttatggctag aaaatatgca agaagttttt ttagtgttga aaacaaatgt
     3901 cattatatgt tcttagttgt attgagtcag agtgttacga agttaggaac tggactacta
     3961 atcagatttt gtgttttctc acagaaatgt tatcagagtc acttttactt ctctgtgagt
     4021 gatagtaaga gcatgactca gtctaaacac atgaaggtgg gggttgtcaa ataacagcag
     4081 aaaggcagtg gctcagaaga cacagaaagg ggccatctta gtccatacaa atgtgccttc
     4141 ccttagactg acagggtctg agtttatctt gtatgtcttg gttcttttgg ttggttggtt
     4201 ggttggttgg ttggttggtt ttatttggtt ttggttttgg ctttagtttt gtttgtttgt
     4261 ttgtttgttt ggattttttt tgtttttttt ttgagatacg gtcttactgt gtgacccaga
     4321 ctgacctcag atgcacacag agccatcagc ctcccacatg ctgggattag aggtatgtgc
     4381 caccacaccc ttcagccctt ttctttttga tcaaagaatt cctgggctca agttatcttt
     4441 ccagcttagc ctcagaagta gctacaacta catgtgggcg ccagcttggg caggtgagcc
     4501 ttttctttaa gcctgatgtt agtctcactt ggatatttac cttcttatgt caagcagaat
     4561 aagggtggaa cacgcttttc ttctggctct tgtttatttt acaaggctac ttaggttagc
     4621 agggatggga agaaaaacat tgctaatcca gttgggataa acgtacgttt gagcttataa
     4681 agcaaggacc acaccacgtt gtgtctctca gctgcaatcc ctgtgtgtat ggctgtgccc
     4741 cagaagtcac accatgctgg tggtgaataa gtgagggctg atttccagtg tgtgccaaga
     4801 agtaacatga ttgaggtagg tgaacctaga gataaaagag ttcttatgat ttagtattga
     4861 aaaaattctg gttttacgat gttgcatttg attcttttca cttgaaaagt catgttttaa
     4921 agctgtaaat tgcctatgtc taaggattta gtaaaatgtc agagatcaat ctataagaaa
     4981 attcaaacat tgtttttata tcttagtagt atgtcattat tttagacctc acaatcccta
     5041 atttaaccat ttcggcaagt gcctttgaca catacggagt attatgggag atgagcaaat
     5101 gtagtgccac accccgtttc ccggcttctc aatgcctcgt gatggtatat gtttaacagt
     5161 gctgcacagt tcagttcttt gtgttcatac gagattgtat tgtagccttt ccatagcaaa
     5221 cagcaaaata aaccattttg cagaaaatga aaa
//
