LOCUS       NM_146216               3124 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript
            variant 1, mRNA.
ACCESSION   NM_146216 NM_138588
VERSION     NM_146216.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Cao,X., Lenk,G.M., Mikusevic,V., Mindell,J.A. and Meisler,M.H.
  TITLE     The chloride antiporter CLCN7 is a modifier of lysosome dysfunction
            in FIG4 and VAC14 mutants
  JOURNAL   PLoS Genet 19 (6), e1010800 (2023)
   PUBMED   37363915
  REMARK    GeneRIF: The chloride antiporter CLCN7 is a modifier of lysosome
            dysfunction in FIG4 and VAC14 mutants.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3124)
  AUTHORS   Mayer,L., Jasztal,M., Pardo,M., Aguera de Haro,S., Collins,J.,
            Bariana,T.K., Smethurst,P.A., Grassi,L., Petersen,R., Nurden,P.,
            Favier,R., Yu,L., Meacham,S., Astle,W.J., Choudhary,J., Yue,W.W.,
            Ouwehand,W.H. and Guerrero,J.A.
  TITLE     Nbeal2 interacts with Dock7, Sec16a, and Vac14
  JOURNAL   Blood 131 (9), 1000-1011 (2018)
   PUBMED   29187380
REFERENCE   4  (bases 1 to 3124)
  AUTHORS   Li,X., Rydzewski,N., Hider,A., Zhang,X., Yang,J., Wang,W., Gao,Q.,
            Cheng,X. and Xu,H.
  TITLE     A molecular mechanism to regulate lysosome motility for lysosome
            positioning and tubulation
  JOURNAL   Nat Cell Biol 18 (4), 404-417 (2016)
   PUBMED   26950892
REFERENCE   5  (bases 1 to 3124)
  AUTHORS   Mironova,Y.A., Lenk,G.M., Lin,J.P., Lee,S.J., Twiss,J.L.,
            Vaccari,I., Bolino,A., Havton,L.A., Min,S.H., Abrams,C.S.,
            Shrager,P., Meisler,M.H. and Giger,R.J.
  TITLE     PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by
            intrinsic and extrinsic mechanisms
  JOURNAL   Elife 5, e13023 (2016)
   PUBMED   27008179
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3124)
  AUTHORS   Jin,N., Chow,C.Y., Liu,L., Zolov,S.N., Bronson,R., Davisson,M.,
            Petersen,J.L., Zhang,Y., Park,S., Duex,J.E., Goldowitz,D.,
            Meisler,M.H. and Weisman,L.S.
  TITLE     VAC14 nucleates a protein complex essential for the acute
            interconversion of PI3P and PI(3,5)P(2) in yeast and mouse
  JOURNAL   EMBO J 27 (24), 3221-3234 (2008)
   PUBMED   19037259
  REMARK    GeneRIF: The authors show that Vac14 exerts an effect as a scaffold
            for the PI(3,5)P2 regulatory complex by direct contact with the
            known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
REFERENCE   7  (bases 1 to 3124)
  AUTHORS   Zhang,Y., Zolov,S.N., Chow,C.Y., Slutsky,S.G., Richardson,S.C.,
            Piper,R.C., Yang,B., Nau,J.J., Westrick,R.J., Morrison,S.J.,
            Meisler,M.H. and Weisman,L.S.
  TITLE     Loss of Vac14, a regulator of the signaling lipid
            phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration
            in mice
  JOURNAL   Proc Natl Acad Sci U S A 104 (44), 17518-17523 (2007)
   PUBMED   17956977
  REMARK    GeneRIF: report the characterization of a mouse mutant lacking
            Vac14, a regulator of PI(3,5)P(2) synthesis
REFERENCE   8  (bases 1 to 3124)
  AUTHORS   Ikonomov,O.C., Sbrissa,D., Dondapati,R. and Shisheva,A.
  TITLE     ArPIKfyve-PIKfyve interaction and role in insulin-regulated GLUT4
            translocation and glucose transport in 3T3-L1 adipocytes
  JOURNAL   Exp Cell Res 313 (11), 2404-2416 (2007)
   PUBMED   17475247
  REMARK    GeneRIF: The data demonstrate for the first time a physical
            association between functionally related PIKfyve and ArPIKfyve in
            3T3-L1 adipocytes.
REFERENCE   9  (bases 1 to 3124)
  AUTHORS   Sbrissa,D., Ikonomov,O.C., Strakova,J., Dondapati,R., Mlak,K.,
            Deeb,R., Silver,R. and Shisheva,A.
  TITLE     A mammalian ortholog of Saccharomyces cerevisiae Vac14 that
            associates with and up-regulates PIKfyve phosphoinositide 5-kinase
            activity
  JOURNAL   Mol Cell Biol 24 (23), 10437-10447 (2004)
   PUBMED   15542851
REFERENCE   10 (bases 1 to 3124)
  AUTHORS   Bonangelino,C.J., Nau,J.J., Duex,J.E., Brinkman,M., Wurmser,A.E.,
            Gary,J.D., Emr,S.D. and Weisman,L.S.
  TITLE     Osmotic stress-induced increase of phosphatidylinositol
            3,5-bisphosphate requires Vac14p, an activator of the lipid kinase
            Fab1p
  JOURNAL   J Cell Biol 156 (6), 1015-1028 (2002)
   PUBMED   11889142
  REMARK    GeneRIF: This publication pertains to studies in yeast.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069308.21 and BC052199.1.
            
            On Oct 26, 2017 this sequence version replaced NM_146216.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052199.1, AK046826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC069308.21        222006-222076
            72-3124             BC052199.1         1-3053
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.59 cM"
     gene            1..3124
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /note="Vac14 homolog (S. cerevisiae)"
                     /db_xref="GeneID:234729"
                     /db_xref="MGI:MGI:2157980"
     exon            1..427
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..2672
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /note="Tax1 (human T-cell leukemia virus type I) binding
                     protein 1; Tax1 (human T-cell leukemia virus type I)
                     binding protein 2; vacuole morphology 14"
                     /codon_start=1
                     /product="protein VAC14 homolog"
                     /protein_id="NP_666328.2"
                     /db_xref="CCDS:CCDS22663.1"
                     /db_xref="GeneID:234729"
                     /db_xref="MGI:MGI:2157980"
                     /translation="MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQN
                     NTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCF
                     NDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRL
                     LKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEIL
                     DGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQL
                     TAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTP
                     EDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAP
                     VTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQT
                     LSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNP
                     PSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENIF
                     HSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLQTPESQNLFCCLYRS
                     WCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLRL
                     QLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDCLKAAPKSQK
                     GDSPSIDYTELLQHFEKVQKQHLEVRHQRSGRGDHLDRRVIL"
     exon            428..578
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            579..746
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            747..809
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            810..917
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1027
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1134
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1269
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1422
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1483
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1628
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1694
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1851
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..1984
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2159
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2278
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2358
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2509
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..3101
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3081..3086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /note="hexamer: AAGAAA"
     polyA_site      3101
                     /gene="Vac14"
                     /gene_synonym="D8Wsu151e; ingls; Tax1bp2; Trx"
                     /note="major polyA site"
ORIGIN      
        1 ggtcggcgga aggcggaagt gctcgcggcg tcacgtgacc tgagtaacat ggccgcggcc
       61 cagtgagcgt tgccggttcc tggcgggagc tggtcgcgtg agccgtgggg cggaccgggt
      121 cgcgcgtggg agagcagggt cggagcaggg ggacccgagg cggcgcgtgg cggcgggacg
      181 aggcaggcgg gccctgggcg gcatagccca tgagcaggct ccggtgtccg cgcggagccg
      241 cacactcccc cggccctggc acggcccagg caacctcggg ctaaggtgtc tggaggctcc
      301 caggcgcagc gcgagctgcc accatgaacc cagagaagga ttttgcgccg ctcactccca
      361 acatcgtaag ggccctgaat gataaactct acgaaaagcg gaaggttgca gcgctggaga
      421 tcgagaagct ggtgcgggac tttgtggccc agaacaacac catgcaaatc aagcacgtga
      481 tccagaccct gtctcaggag tttgccctgt cccagcaccc ccacagccgg aaagggggcc
      541 tcatcggtct ggcagcctgc tctattgccc taggcaagga ctcagggctc tacctgaagg
      601 agctgataga gccagtgctg acctgcttta atgacgcaga cagcaggctg cgctactacg
      661 cttgtgaggc cctctacaat atcgtcaaag tggcccgagg tgccgtgctg ccgcacttca
      721 atgttctctt cgatggactg agtaagttgg ctgctgaccc agaccccaat gtgaaaagtg
      781 gatctgagct cctggaccgc ctcttaaagg acattgtgac agaaagcagc aagtttgacc
      841 tggtgagctt catccccctt ttgcgggaga ggatttactc caacaaccaa tacgcccgac
      901 agttcatcat ctcctggatc ctggttctgg tgtcggtgcc agacattaac ctgctggatt
      961 acctgccaga gatcctagat gggctcttcc agatcctggg tgacaatggc aaggagattc
     1021 ggaaaatgtg tgaggtggtt cttggagagt tcttgaaaga aattaagaag aacccatcca
     1081 gtgtgaagtt tgctgagatg gccaacatcc tggtgatcca ctgccagacc acagatgacc
     1141 tgattcagct gacggctatg tgctggatgc gggaattcat ccagctggcg ggccgggtaa
     1201 tgctgcccta ttcctctggg atcctgactg ctgtcctgcc atgcttggcc tatgatgacc
     1261 gcaagaaaag tatcaaggag gtggccaatg tgtgtaacca gagcttgatg aagctggtca
     1321 cccctgagga tgatgaaccc gatgagccaa agtcagtagc acagaagcag accgaaccca
     1381 accctgagga ttccctgcca aagcaggagg ggacagccag tggaggtcca ggttcctgtg
     1441 actccagctt tggaagtggc atcaatgtct tcacctcagc caacactgat agggctccag
     1501 tgaccctgca cctggacggg attgttcaag tcctgaactg tcaccttagt gacacaacca
     1561 ttgggatgat gaccaggatt gctgttctga agtggctcta ccatctctac atcaaaactc
     1621 ctcgaaagat gttccggcac acagacagcc tcttccccat cttacttcag acattatctg
     1681 acgaatctga tgaggttgtc ttgaaggatc tggaagtgtt ggcagaaatt gcttcctccc
     1741 ctgcaggcca gacggatgac ccaggtgcac ctgatggccc tgacctccga gtcaaccact
     1801 cggagcttca ggtgcccacc tccggcagag ccaacctact aaaccctccc agtaccaaag
     1861 gcttagaagg ctccccctcc actcccacca tgaactccta cttttacaag ttcatgatca
     1921 accttctaca gacgttcagc agtgaacgga agctcctgga ggccagaggc ccgttcatca
     1981 tcaggcagct ctgcctcctg ctgaatgcag agaacatctt ccattcaatg gcagacatcc
     2041 tgcttcggga ggaggacctc aagttcgcgt ccaccatggt gcacaccctc aacaccatcc
     2101 tgctcacctc caccgagctc ttccagctga ggaaccagct caaggacctg cagaccccgg
     2161 aaagccagaa cctcttctgc tgcctgtacc gctcctggtg ccacaaccca gtcaccaccg
     2221 tgtccctctg cttcctcacc cagaactacc gccacgccta tgacctcatc cagaagtttg
     2281 gggacctgga agtcacagtg gatttcctca cagaggtgga caaactcgtg cagctaattg
     2341 agtgccccat ctttacatat ctacgcctgc aactgctgga cgtgaagaac aacccatacc
     2401 tgatcaaggc cctgtacggc ctgctcatgc tgttgcccca gagcagtgcc ttccagctgc
     2461 tctctcaccg gctccagtgt gtgcccaacc ctgagctgct gcagactgaa gactgtctga
     2521 aagcggcccc caagtcccag aaaggtgact cccccagcat cgactacaca gagctgctgc
     2581 agcactttga aaaagtccag aaacagcacc tggaagtaag acaccagcgc agtgggcgag
     2641 gggatcacct ggaccgcaga gttatcctct gatgtgccta gcatggaaaa gggccctagt
     2701 ggcctaagaa gcactcaggg tctctaagct gaggccacaa ggagcctaag aacctgcctc
     2761 cagcagggct ggacccaacc accagccagg gtagggaggg ggatggtatt ggctctgtca
     2821 gcctcagctc ctcccagcag agcacctgga ccccagcact gtcagctaca ctctgtcctg
     2881 ggaccgatgg attctctcct gagtcgggac tacagcctca gaatccagaa gcagcctctt
     2941 tctaatccct gtctgtgaga agagcttgac cttggcccca gagcactggc atggtgtgtg
     3001 tgcatatgta catctgtgtg gaaatgcaca caggccagca caaagacctc tacagaatga
     3061 tgtccatccc tgccccaata aagaaatcac agaatcctta aaaaaaaaaa aaaaaaaaaa
     3121 aaaa
//
