LOCUS       NM_177387               4284 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus uronyl-2-sulfotransferase (Ust), mRNA.
ACCESSION   NM_177387
VERSION     NM_177387.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4284)
  AUTHORS   Schaberg,E., Theocharidis,U., May,M., Lessmann,K., Schroeder,T. and
            Faissner,A.
  TITLE     Sulfation of Glycosaminoglycans Modulates the Cell Cycle of
            Embryonic Mouse Spinal Cord Neural Stem Cells
  JOURNAL   Front Cell Dev Biol 9, 643060 (2021)
   PUBMED   34169071
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4284)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   5  (bases 1 to 4284)
  AUTHORS   Paul,A., Crow,M., Raudales,R., He,M., Gillis,J. and Huang,Z.J.
  TITLE     Transcriptional Architecture of Synaptic Communication Delineates
            GABAergic Neuron Identity
  JOURNAL   Cell 171 (3), 522-539 (2017)
   PUBMED   28942923
REFERENCE   6  (bases 1 to 4284)
  AUTHORS   Nikolovska,K., Spillmann,D., Haier,J., Ladanyi,A., Stock,C. and
            Seidler,D.G.
  TITLE     Melanoma Cell Adhesion and Migration Is Modulated by the Uronyl 2-O
            Sulfotransferase
  JOURNAL   PLoS One 12 (1), e0170054 (2017)
   PUBMED   28107390
  REMARK    GeneRIF: This is the first study showing that Ust, and consequently
            2-O sulfation of the low affinity receptor for FgfR CS/DS, reduces
            Itga5 and leads to an impaired adhesion and migration of melanoma
            cells
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4284)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   8  (bases 1 to 4284)
  AUTHORS   Nishimura,K., Ishii,M., Kuraoka,M., Kamimura,K. and Maeda,N.
  TITLE     Opposing functions of chondroitin sulfate and heparan sulfate
            during early neuronal polarization
  JOURNAL   Neuroscience 169 (4), 1535-1547 (2010)
   PUBMED   20600662
REFERENCE   9  (bases 1 to 4284)
  AUTHORS   Ishii,M. and Maeda,N.
  TITLE     Oversulfated chondroitin sulfate plays critical roles in the
            neuronal migration in the cerebral cortex
  JOURNAL   J Biol Chem 283 (47), 32610-32620 (2008)
   PUBMED   18819920
  REMARK    GeneRIF: analysis of the three CS sulfotransferases in the
            developing neocortex: uronyl 2-O-sulfotransferase (UST),
            N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (4,6-ST) and
            dermatan 4-O-sulfotransferase-1 (D4-ST)
REFERENCE   10 (bases 1 to 4284)
  AUTHORS   Akita,K., von Holst,A., Furukawa,Y., Mikami,T., Sugahara,K. and
            Faissner,A.
  TITLE     Expression of multiple chondroitin/dermatan sulfotransferases in
            the neurogenic regions of the embryonic and adult central nervous
            system implies that complex chondroitin sulfates have a role in
            neural stem cell maintenance
  JOURNAL   Stem Cells 26 (3), 798-809 (2008)
   PUBMED   18079434
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147850.1, AK162950.1 and AC159466.3.
            
            On Jul 19, 2006 this sequence version replaced NM_177387.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147850.1, AK086181.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1208              AK147850.1         2-1209
            1209-1773           AK162950.1         782-1346
            1774-4284           AC159466.3         160880-163390       c
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 2.65 cM"
     gene            1..4284
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /note="uronyl-2-sulfotransferase"
                     /db_xref="GeneID:338362"
                     /db_xref="MGI:MGI:2442406"
     exon            1..676
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..1650
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /codon_start=1
                     /product="uronyl 2-sulfotransferase"
                     /protein_id="NP_796361.2"
                     /db_xref="CCDS:CCDS35835.1"
                     /db_xref="GeneID:338362"
                     /db_xref="MGI:MGI:2442406"
                     /translation="MKKKQQQHPGGGTDPWPHGAPVGGAPPCLGSCKRRIPLLPFLRF
                     SLRDYGFCMATLLVFCLGSLFYQLSGGPPRFLLDLRQYLGNSTYLDDHGPPPSKVLPF
                     PSQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHNKTRLTKNEQMELIKNISTA
                     EQPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVSRFLSNYFFRRFGDWRGEQNHMIRT
                     PSMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVN
                     ENFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVRKTVPSPEA
                     VQILYQRMRYEYEFYHYVREQFHLLKRKLGLKSRVSGPPVRPQFFIPTPLETEEPIDD
                     EEQDDEKWLEDIYKR"
     exon            677..720
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            721..876
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            877..956
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1110
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1208
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1366
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..4284
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4149..4154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /note="hexamer: AATAAA"
     polyA_site      4170
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /note="major polyA site"
     regulatory      4207..4212
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /note="hexamer: AATAAA"
     polyA_site      4230
                     /gene="Ust"
                     /gene_synonym="D930010O20Rik; UA2OST"
                     /note="major polyA site"
ORIGIN      
        1 gccttgagct agccgagcct gaactgggac caaacacacc gcccgcgacc cacacaaagc
       61 ccggggggtc cacgctggcg ctggaggcga gccgggagcg agcccggggc gcggcgggac
      121 ttggggcgca gggctgctac gggacgcggg gctgctagca ggacgcgcga ccaggcgtgg
      181 gttgcgatcg ccacccccac ggcggggccc gggacatttt gggccctttg gggccgcagc
      241 gagcggtggg gaccaagacg agcagagtac cctgagcccg gcaacttcgg cccctccccg
      301 cccccacccg gctgccctca gcgcgtccct ctccatgtgc agacagccgg ctaggcgctc
      361 cggctggcgg cggatgggtg acctcttcct ggccggggcg ggctgttcga ggcggcggag
      421 ccggcgatga agaagaagca gcagcagcat cccggcggcg gcacggatcc ctggccccat
      481 ggggcccctg tggggggcgc ccctccgtgc ctgggcagct gcaagcgccg gatcccgctg
      541 ctgcctttcc tgcgcttctc cctccgggac tacggtttct gcatggctac cctgctggtc
      601 ttctgcctgg gctccctctt ctaccagctc agcgggggac cccctcgctt cctgctcgac
      661 ctgcggcagt atttgggaaa ttccacttac ttggatgacc atggaccacc tcctagtaag
      721 gtccttccct tccccagcca ggtggtgtac aacagagttg gcaagtgtgg cagtcgtacc
      781 gtggtcttgc ttctgagaat cttgtcagag aagcacggct tcaatttggt cacatcagac
      841 attcacaaca agacccggct tactaaaaat gaacaaatgg aactgattaa gaatataagt
      901 accgccgaac agccctattt attcacgaga catgtccact tcctcaactt ctccaggttt
      961 ggaggagacc agcctgtcta catcaacatc atcagagacc ccgtcagtag atttctgtct
     1021 aactatttct tccgtcgctt tggagattgg agaggggaac agaatcacat gatccgcacc
     1081 cccagcatga ggcaggagga gcgctacctg gacatcaatg agtgcattct ggaaaactac
     1141 cctgagtgtt ccaaccccag gctgttttac atcatcccat atttctgtgg acagcacccc
     1201 agatgtaggg agcccggcga gtgggcgctg gagcgggcca agctgaacgt gaacgaaaac
     1261 ttcctgctcg tggggatcct ggaggagctg gaagatgtgc tgctcttact ggaaagattt
     1321 ttacctcatt acttcaaggg tgtgctgagc atctacaagg acccagagca caggaaactt
     1381 ggcaacatga ctgtgaccgt gaggaagact gtcccctccc ccgaggcggt gcagattctc
     1441 taccagcgaa tgagatacga gtacgagttc taccactacg tcagagaaca attccacctg
     1501 ctcaagcgca agcttggact gaagtctcgc gtgagcggac ctcccgtgag accacagttc
     1561 ttcatcccca cgcccctgga gacggaggag ccaatcgacg acgaggagca agatgacgaa
     1621 aaatggctag aagatattta taagaggtga tacctgcgcc agattgtggc ttctgtggcc
     1681 tcatcccccc ttttacaaag ttctttgggg aagagaaatt ctgaagggac tttcattaat
     1741 gctcgagtgc attaaaaaga acaaaacatt cctacatgtt ggggtcattg ggagatgccc
     1801 ggttttgcgg gttttatttg tttaatttta ttctgtgtgg tttttcttcc ctccttgagg
     1861 ttttcccaga cacactagat ggctctacca caaggcacct cttcagaaaa taggtagtcc
     1921 cctacctgac actttcagtg aaagaagaga gagatatacc ctccatctta gtcattttat
     1981 catttgtaaa atggctccaa aaatattaca tccgtggtaa agacatcctg atgtgtatct
     2041 cgcagaaata accatttcag agaggaaggg gtctagaagc atctaggagt agaaggaaga
     2101 caagagggag gaatccactg gttctggggt ttctaccccc accccacctc accccgtcgg
     2161 tagaatgggg acactgtcac caagaacaca gtacttatct ctgagagaac aagtcagtgt
     2221 ccagcatcca ccttctcaac aaatcagagc tagggaagct tgccgagatc aagctgtgtg
     2281 cacagtaagc tccattgaga acttccattc ccaggtatcg ctctgcttgg aaggcattcc
     2341 ctggaaggcg tcagcttgtt atgtcacgtg ttggcagggc agatcaaaca taaggacctc
     2401 attgttgcac gtgcaagtgc cagaaactga ctgacttctg accttgatat gtgtggcagg
     2461 aatccttagc tcaattgcac accgtgtgcg atcctgaaag cacactcata gccaatgggg
     2521 ctatactgaa cttggcattt ttcattcctt ctagacctgc aggagctgtt gtgtttggta
     2581 actaagggtt ccaagtcctg ccctagcaac agtagaacca ttttcttata gaacctgttt
     2641 gcacccataa cccttcccag ggacaccaat gccagagacc tcaatgccac agtgtctggg
     2701 caggaccctg cttggctagg gtagagtaga ttggtgtccc aacatgatct gagtaaacac
     2761 tgggcaattc ctttgcacca aaatagtcct tgcacctact tactcctgac aactctccac
     2821 tacccatgat gctctgctcc tttaattagt gaattgtgtc ttttttttta agtcatgaaa
     2881 attttaaaat ttcatattct cactggttga tagaatgcaa ctagagaggc cagactgcag
     2941 tgatggcagt gggaaggaca tttggtgaca acttgtgtct gcctcaggtt taccccagag
     3001 cctgccattg aagaagacag ccaagtgagt tagaagtact taagataaac aagactctgg
     3061 cttggcacac caggcacaca ttttgtactt tacagaaacc atcaagtttc tctagaattt
     3121 tgttttcttc cttcactaaa actggacact ggccaaggtc taaagcccgt catctgcaaa
     3181 actgcccggt ctatactgaa gctccttcat catgaaatgc attaagacag gtccccgctc
     3241 aaaggaaagg cagggaccac agaggtagag aagtctagca ctggccactc atgggcctcc
     3301 tttctgcctc agggaactgg gacaggaagt ggataaggga gctgttcaaa gagggggaaa
     3361 taacctttgt cctgtgctgg gcctttccat taatcagatt ttgaagagca acatagaaaa
     3421 cacctgctac cattctccaa acaagtatga ggactgcagt tgcagggctt tgtaagtcgt
     3481 ctccatggta ttccatacac agtgatggcg atggttagct gaccgagatc ccaccatcaa
     3541 agataaagct gtgttagctg gactttctcg gctgttgtcc aaacgtgggg acagctggtc
     3601 actcataaag gtcccctgct ttctagaggt gcacatagca cttgctgtgt gttcgaattg
     3661 ccacccatgg ccactgtcac accatgggtg agggggttgg ctgactggtt gcaggaccct
     3721 cctggtatct tggcctagca cactgcccct acaccctaaa aagtcaaggt acttcatcag
     3781 ctcgcttggg ccctggccca tcatagtttg tacttcatga aatatattgt acattttaca
     3841 tagtttaatt taaaaagaaa atacatttta agctagttgg tccttgcctt atttattata
     3901 acctttcagc acattccaag gttttagtta ctcaggaagg agttaattaa aatgatttta
     3961 ttttggtcgg atgggtgttt tttttttttt aaggaaaaaa tattcttatg aaccctcggc
     4021 tggctctcct ttgaatgctg taaagtgctc gtgagtctgt cttttttttt tttaagtatt
     4081 aaaaaatggt gtttcacatt gatggaagtt caaaaatata tatggaacta aagcattagc
     4141 ttagctataa taaaaacagt ctgtgtctga ggtgaagtgt tccttaattt attcattacc
     4201 catagaaata aatgaatata aataagtgga ccttattttt tctgttattt tggtagctgg
     4261 aaattaaaga cttcctttct caag
//
