LOCUS       NM_001252580            4210 bp    mRNA    linear   ROD 05-MAR-2023
DEFINITION  Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript
            variant 1, mRNA.
ACCESSION   NM_001252580
VERSION     NM_001252580.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Bi W, Zhang J, Zeng Z, Zhou R, Zhao J, Yan W, Wang L, Li X and Zhu
            L.
  TITLE     Ubiquitin-specific protease 8 ameliorates
            lipopolysaccharide-induced spleen injury via suppression of
            NF-kappaB and MAPK signaling pathways
  JOURNAL   Mol Med Rep 26 (6) (2022)
   PUBMED   36321796
  REMARK    GeneRIF: Ubiquitinspecific protease 8 ameliorates
            lipopolysaccharideinduced spleen injury via suppression of NFkappaB
            and MAPK signaling pathways.
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Xiong W, Gao X, Zhang T, Jiang B, Hu MM, Bu X, Gao Y, Zhang LZ,
            Xiao BL, He C, Sun Y, Li H, Shi J, Xiao X, Xiang B, Xie C, Chen G,
            Zhang H, Wei W, Freeman GJ, Shu HB, Wang H and Zhang J.
  TITLE     USP8 inhibition reshapes an inflamed tumor microenvironment that
            potentiates the immunotherapy
  JOURNAL   Nat Commun 13 (1), 1700 (2022)
   PUBMED   35361799
  REMARK    GeneRIF: USP8 inhibition reshapes an inflamed tumor
            microenvironment that potentiates the immunotherapy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4210)
  AUTHORS   Chaugule S, Kim JM, Yang YS, Knobeloch KP, He X and Shim JH.
  TITLE     Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by
            Regulating Wnt Signaling
  JOURNAL   Int J Mol Sci 22 (19), 10289 (2021)
   PUBMED   34638628
  REMARK    GeneRIF: Deubiquitinating Enzyme USP8 Is Essential for
            Skeletogenesis by Regulating Wnt Signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4210)
  AUTHORS   Bland T, Sahin GS, Zhu M, Dillon C, Impey S, Appleyard SM and
            Wayman GA.
  TITLE     USP8 Deubiquitinates the Leptin Receptor and Is Necessary for
            Leptin-Mediated Synapse Formation
  JOURNAL   Endocrinology 160 (8), 1982-1998 (2019)
   PUBMED   31199479
REFERENCE   5  (bases 1 to 4210)
  AUTHORS   Gu H, Shi X, Liu C, Wang C, Sui N, Zhao Y, Gong J, Wang F, Zhang H,
            Li W and Zhao T.
  TITLE     USP8 maintains embryonic stem cell stemness via deubiquitination of
            EPG5
  JOURNAL   Nat Commun 10 (1), 1465 (2019)
   PUBMED   30931944
  REMARK    GeneRIF: Deubiquitination of EPG5 by USP8 guards the autophagic
            flux in embryonic stem cells (ESCs) to maintain their stemness.
            This work uncovers a novel crosstalk pathway between ubiquitination
            and autophagy through USP8-EPG5 interaction to regulate the
            stemness of ESCs.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4210)
  AUTHORS   Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S,
            Nakajima D, Nagase T, Ohara O and Koga H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   7  (bases 1 to 4210)
  AUTHORS   Berruti G and Martegani E.
  TITLE     mUBPy and MSJ-1, a deubiquitinating enzyme and a molecular
            chaperone specifically expressed in testis, associate with the
            acrosome and centrosome in mouse germ cells
  JOURNAL   Ann N Y Acad Sci 973, 5-7 (2002)
   PUBMED   12485825
  REMARK    GeneRIF: deubiquitinating enzyme specifically expressed in testis,
            associates with the acrosome and centrosome in mouse germ cells
REFERENCE   8  (bases 1 to 4210)
  AUTHORS   Berry DM, Nash P, Liu SK, Pawson T and McGlade CJ.
  TITLE     A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity
            for the interaction between Gads and SLP-76 in T cell signaling
  JOURNAL   Curr Biol 12 (15), 1336-1341 (2002)
   PUBMED   12176364
REFERENCE   9  (bases 1 to 4210)
  AUTHORS   Gnesutta N, Ceriani M, Innocenti M, Mauri I, Zippel R, Sturani E,
            Borgonovo B, Berruti G and Martegani E.
  TITLE     Cloning and characterization of mouse UBPy, a deubiquitinating
            enzyme that interacts with the ras guanine nucleotide exchange
            factor CDC25(Mm)/Ras-GRF1
  JOURNAL   J Biol Chem 276 (42), 39448-39454 (2001)
   PUBMED   11500497
REFERENCE   10 (bases 1 to 4210)
  AUTHORS   Kato M, Miyazawa K and Kitamura N.
  TITLE     A deubiquitinating enzyme UBPY interacts with the Src homology 3
            domain of Hrs-binding protein via a novel binding motif
            PX(V/I)(D/N)RXXKP
  JOURNAL   J Biol Chem 275 (48), 37481-37487 (2000)
   PUBMED   10982817
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY147904.1, AK129003.1 and BY639666.1.
            
            [WARNING] On Jun 2, 2023 this sequence was replaced by
            NM_001252580.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129003.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BY147904.1         1-32
            33-4186             AK129003.1         1-4154
            4187-4210           BY639666.1         376-399
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..4210
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /note="ubiquitin specific peptidase 8"
                     /db_xref="GeneID:84092"
                     /db_xref="MGI:MGI:1934029"
     exon            1..160
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            161..330
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..3502
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative deubiquitinating enzyme; ubiquitin
                     carboxyl-terminal hydrolase 8; ubiquitin thioesterase 8;
                     deubiquitinating enzyme 8; ubiquitin-specific-processing
                     protease 8; ubiquitin isopeptidase Y; ubiquitin
                     thiolesterase 8"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 8 isoform
                     1"
                     /protein_id="NP_001239509.1"
                     /db_xref="CCDS:CCDS57185.1"
                     /db_xref="GeneID:84092"
                     /db_xref="MGI:MGI:1934029"
                     /translation="MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNTKFCKFSSF
                     LSYIHSAQKIFKTAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILG
                     PANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAA
                     AKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKI
                     QDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSA
                     KDLLLGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRS
                     RAEEVSVSLDFTYPSLEEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALA
                     GPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSENTDQSGRVLSDRS
                     TKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRREEQERKA
                     GKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEA
                     SDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGL
                     PLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDRE
                     PSKLKRSYSSPDITQALQEEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNP
                     VFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGE
                     VAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHED
                     LNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRR
                     RSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIE
                     IWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVS
                     NHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITD
                     VAT"
     exon            331..508
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            509..594
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            595..757
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            758..800
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            801..945
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1108
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1253
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1477
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..2035
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2116
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2379
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2592
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2803
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..3040
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3183
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3316
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..4210
                     /gene="Usp8"
                     /gene_synonym="mKIAA0055; Ubpy"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgacgtttc gagacgcgac ccgtcgccac gcaatgcgaa tcgggaagag gggatgagct
       61 gcgctgccgt gcgtctgaga gcgaaggctc gttctccttc tggtggtggg cgatgggcgt
      121 caggcgagga ggccggtgtt agcggagcgc gggcgctgcg gaaagaacta cttttcatga
      181 accacaccat ctgtttgttg aagtagaaaa gcgagtctta ctcatcatgc ctgctgtagc
      241 ttcagttcct aaagaactct acctcagttc ttcactaaaa gacctcaata agaagaccga
      301 agttaaacct gagaaaacca gcaccaagaa taccaagttt tgtaaattct cttcgtttct
      361 aagttatata cacagcgcgc agaagatctt caagacagca gaagaatgca gactagatcg
      421 tgatgaggaa agggcctatg tgctttatat gaaatatgtg gcagtttata atcttatcaa
      481 aaagagacct gatttcaagc aacagcagga ttattatctt tcaatacttg gacctgcaaa
      541 catcaaaaaa gctattgaag aagctgaaag actctccgaa agccttaaac taagatacga
      601 agaggctgaa gttcggaaac aacttgaaga aaaggacaga cgggaggaag agcagctgca
      661 gcaacagaaa aggcaggaga tggggagaga ggacagcggt gcggcagcca aacgctctgt
      721 ggaaaattta ctggattcca aaaccaaaac ccaaaggatt aacggcgaga agagtgaagg
      781 agctgcagct gcagagagag gagccatcac agcaaaggaa ctatatacga tgatgatgga
      841 taaaaacaca agcttaatta taatggatgc tcgaaaaata caggattatc agcattcctg
      901 tatcttggat tctctcagtg ttcctgaaga agctatcagt ccaggagtca ctgctagttg
      961 gattgaagca aacctctcag atgattctaa agacacatgg aaaaagaggg ggagtgtgga
     1021 ctatgtggtc cttctcgact ggtttagttc agcgaaagat ttgctgcttg ggaccactct
     1081 acggagtctg aaagatgcac ttttcaagtg ggaaagtaaa actgtcctgc gccatgagcc
     1141 tttggtgttg gagggcggct atgaaaactg gctgctttgc tacccgcagt ttacaaccaa
     1201 tgctaaggtc actccacccc ctcggagcag agctgaagag gtgtctgtct cattggattt
     1261 tacttatccc tcattggaag aaccagttcc ttccaaactt cctacccaga tgccacctcc
     1321 tcctatagaa acaaatgaaa aggcactgtt ggtaactgat caagatgaaa agctgagact
     1381 gtcaacccag ccagctctag ctggacctgg tgcggctccc agagctgaag cctcacccat
     1441 aattcagcca gcgcctgcta caaagagtgt tccacaggtt gatcgtacga aaaaaccgtc
     1501 agtcaagttg cctgaagatc atagaataaa atctgaaaat acagatcaga gtggaagagt
     1561 tctttctgat cgatccacca agccagtatt tccctctcca accaccatgt taacagatga
     1621 agaaaaggct cgtattcatc aagaaactgc ccttcttatg gaaaagaata aacaggagaa
     1681 ggaactttgg gacaagcagc agaaggaaca gaaagagaag ctgagaaggg aggaacaaga
     1741 gcgcaaagct ggaaagacac aggatgcaga tgaacgtgac tccactgaga atcagcacaa
     1801 agcaaaggat ggacaagaga aaaaagacag caaacagacc aagacggaag acagagagct
     1861 ttcagcagac ggggcccagg aagccacagg aacacaaaga caaagtaaga gtgagcatga
     1921 agcttctgat gctaaggtac ctgtggaagg taaaaggtgt cccacgtcag aggcgcagaa
     1981 gaggccagca gatgtgtccc ctgcatccgt gtcaggagag ctgaatgcag gcaaggctca
     2041 gcgagaacct ttgacgagag cacgaagtga agaaatgggg agaattgtgc cgggactgcc
     2101 tttgggctgg gccaagtttc ttgatccaat caccgggacc tttcgttact accattcccc
     2161 cacaaacaca gttcatatgt atccacctga aatggctcct tcgtctgcac ctccttccac
     2221 cccgcccact cacaaagtca agccccaggt ccctgccgag cgggacaggg agccatcgaa
     2281 actgaagcgc tcctactcct caccagatat cactcaggcc ctgcaggagg aggagaagag
     2341 gaggccggca gtgaccccga tggtcaaccg ggaaaacaag ccaccatgtt accctaaagc
     2401 tgagatttcg aggctttctg cttctcagat tcggaacctc aatcctgtat ttggaggatc
     2461 aggaccagct cttactggac ttcgtaattt gggaaatact tgttacatga actcaatctt
     2521 gcagtgcctg tgtaatgctc cacatctggc tgattatttc aaccgaaact gctaccagga
     2581 tgatatcaac aggtcaaatt tgttggggca taaaggtgaa gtggcagaag aatttggtat
     2641 aatcatgaaa gcactgtgga caggacagta tagatacatc agtccaaagg actttaaagt
     2701 caccattggt aagattaatg accagtttgc aggctccagc caacaggatt cacaagagct
     2761 gcttctgttc ctcatggatg gcctgcatga ggatctgaat aaggctgaca atcggaagag
     2821 gcacaaggaa gagaacaacg agcacctgga tgacctgcag gcggccgagc acgcctggca
     2881 gaagcacaag cagctcaacg agtccatcat cgtggccctg ttccagggcc agttcaagtc
     2941 cacagtgcag tgcctcacct gccgcaggag gtcgcgcacc ttcgaggcct tcatgtactt
     3001 gtctttgccg ctagcatcca caagtaaatg tactctacag gactgcctta gattattttc
     3061 caaagaagaa aagcttacag ataacaacag attttactgc agccattgcc gagctcggcg
     3121 ggattctcta aagaaaatag aaatctggaa attacctcct gtgctgttag tgcacctgaa
     3181 acgattttcc tatgacggca ggtggaagca gaagctgcaa acatccgtgg atttcccatt
     3241 ggaaaatctt gacctgtcac agtacgttat tggtccaaaa aacagcttga agaaatataa
     3301 cttgttttct gtttcaaacc actacggcgg gctagacgga ggccactaca cagcctactg
     3361 taagaacgcg gcaaggcagc gctggtttaa gtttgatgac catgaagttt ctgatatctc
     3421 tgtgtcttct gtgaggtcat cagcagctta tatcctcttt tatacttccc tgggaccacg
     3481 cataactgat gtagccacat agagaggagt aggctgtcgg cttattgacg tctccaaatt
     3541 gcagcctcac atctaatgct tacaaagata atggatcgct gcagctgcta tgtagaggga
     3601 ttccagaaca ctggggctgt gctactagca ccatataatt caggccagtg ctgtttatca
     3661 aaagaaagaa agaaagaaaa cagcctgaca tttaacaact atcatagtac gcattcctta
     3721 caggagtcat ttatttcaaa acaacttttt gagtctgccc caaagttaaa ataattcatt
     3781 agtcagacat ttattattct cttggtacca aaccatcata acctccattt tcctattcac
     3841 tgctccagcc tcttctcatt tctagcccca gctcagtgca ctctgaatgc tctagaatga
     3901 cctagctcag ataggaatgt atatgtacat atttattgca ctgttgcaca tcagactgct
     3961 ggacacagtt taacatagcc ttctgtgaaa gcctagaaag gattttttct ttttcttttg
     4021 gcctcttcta ggtagctgca atgctttctt aaggaaatga cagggcaaaa ctatttttct
     4081 gttggcttcc ggctgtattt gtgcactaaa tctttattct aaacaaatgg aaactttaat
     4141 tttttaaaaa taattcattt acatctacat taaaatctta atgaagaaaa aaatcatttg
     4201 aaaccttgta
//
