LOCUS       NM_029163               1748 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin specific peptidase 50 (Usp50), mRNA.
ACCESSION   NM_029163
VERSION     NM_029163.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Zhao,C., Mu,M., Li,X., Dong,Z., Wang,J., Yao,C., Zheng,J., Sun,X.
            and Yu,J.
  TITLE     USP50 regulates NLRP3 inflammasome activation in duodenogastric
            reflux-induced gastric tumorigenesis
  JOURNAL   Front Immunol 15, 1326137 (2024)
   PUBMED   38469295
  REMARK    GeneRIF: USP50 regulates NLRP3 inflammasome activation in
            duodenogastric reflux-induced gastric tumorigenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1748)
  AUTHORS   Lee,J.Y., Seo,D., You,J., Chung,S., Park,J.S., Lee,J.H., Jung,S.M.,
            Lee,Y.S. and Park,S.H.
  TITLE     The deubiquitinating enzyme, ubiquitin-specific peptidase 50,
            regulates inflammasome activation by targeting the ASC adaptor
            protein
  JOURNAL   FEBS Lett 591 (3), 479-490 (2017)
   PUBMED   28094437
  REMARK    GeneRIF: the deubiquitinating enzyme USP50 binds to the ASC protein
            and subsequently regulates the inflammasome signaling pathway
REFERENCE   4  (bases 1 to 1748)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY707071.1, AK015766.1 and AW539155.2.
            
            On Oct 1, 2009 this sequence version replaced NM_029163.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK015766.1, BC061020.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BY707071.1         2-17
            17-637              AK015766.1         2-622
            638-691             BY707071.1         581-634
            692-1376            AK015766.1         677-1361
            1377-1748           AW539155.2         1-372               c
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.76 cM"
     gene            1..1748
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /note="ubiquitin specific peptidase 50"
                     /db_xref="GeneID:75083"
                     /db_xref="MGI:MGI:1922333"
     exon            1..241
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1346
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin specific protease 50; putative ubiquitin
                     carboxyl-terminal hydrolase 50; ubiquitin thioesterase 50;
                     deubiquitinating enzyme 50; ubiquitin-specific-processing
                     protease 50; ubiquitin thiolesterase 50"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 50"
                     /protein_id="NP_083439.2"
                     /db_xref="CCDS:CCDS16688.1"
                     /db_xref="GeneID:75083"
                     /db_xref="MGI:MGI:1922333"
                     /translation="MCFIDMASHRPVPADDFGAYYNLAECADYDSLPESKTQPHFQGV
                     TGLRNLGNTCYMNAILQCLCSVSPLVEYFLSGKYITALKKDCSEVTTAFAYLMTDMWL
                     GDSDCVSPEIFLSAVGSLYPAFLKKTQQDAQEFLIYVLNELHEALKKHCRRRVNEKRT
                     GQSCCRKVPAQETSIITRLFEGQLSYSITCLKCESCTHKNEVFTILSLPIPSDYECSL
                     QDCLQCFFQQDTLTWSNQIYCSFCEIKQEAAVRTTISKVPKIIVFHFKRFDIQGTVKR
                     KLRTDIHYPLTNLDLTPYICPVFRKHPMYNLCAVVNHFGDLDGGHYTAFCKNSVTQAW
                     YSFDDTRVSEIPDTSVQTATAYLLFYSCQPFSIPAQKRKSQDSTPDHCKQAIRKWP"
     exon            242..424
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            425..620
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            621..830
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            831..973
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1106
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1748
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1359..1364
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1377
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /note="major polyA site"
     regulatory      1730..1735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1747
                     /gene="Usp50"
                     /gene_synonym="1700086G18Rik; 4930511O11Rik"
ORIGIN      
        1 aggggaggat ccaagaagaa ctgggcctga gtcaagtgaa atcagacagc aacaacacaa
       61 aggggggagg ggggcattca aacggttcaa aaccaaaaac aagccaggga gcagtcctgg
      121 agaaatgaag ttaaaaggat caaaggtagc tcgtgagtag aagtgaaagc ctgatgtgct
      181 tcattgacat ggcgtctcat cggccggtcc ctgcagacga cttcggtgcc tactacaacc
      241 ttgcggagtg tgcagactat gactccctgc cggagtctaa gacccagcca cacttccagg
      301 gcgtgacggg actgcggaac ctgggcaaca cctgctacat gaacgccatc ttgcaatgcc
      361 tctgcagcgt atctccactg gtggagtact tcctgtccgg aaagtacatc acagctctta
      421 agaaggactg cagtgaggtt accaccgcct ttgcctacct gatgacagat atgtggcttg
      481 gggactcaga ctgtgtctca ccagagatat ttctgtcggc tgttggcagt ctttacccag
      541 cttttctgaa aaagacacag caagatgcgc aggagttcct gatttacgtc ctcaacgagc
      601 ttcacgaagc tttgaaaaag cactgccgaa gaagagtgaa tgagaaaaga acaggccaaa
      661 gctgctgcag gaaggtgccg gcccaggaga cctccatcat cacccggctg tttgaaggac
      721 agctcagtta cagcatcaca tgtttaaagt gtgagagctg cacgcacaag aacgaagttt
      781 tcaccatcct ctccctcccc atcccatctg actatgaatg ttccctccag gactgtctcc
      841 agtgtttttt ccaacaggac acactgacct ggagcaacca aatctattgt tccttttgtg
      901 aaatcaagca agaggcagct gtgaggacca cgatttccaa ggtgccgaaa ataattgttt
      961 ttcactttaa aaggtttgac atccagggca cagtaaagag gaagctgcgg acagacatcc
     1021 attaccccct gaccaacctg gacctcactc cttatatctg tccagttttc cggaaacatc
     1081 ccatgtacaa cctgtgcgca gtggtgaacc actttggaga tctggatggt ggtcactaca
     1141 ctgctttctg caagaactcg gtaactcagg cctggtacag ctttgatgac acccgagtca
     1201 gtgagatccc agatacttct gttcaaacag ctacagccta tcttctgttc tatagctgcc
     1261 agcctttttc tataccagca cagaaacgca agagccagga cagtactcct gaccactgca
     1321 agcaagctat ccgaaagtgg ccgtgaatat gctttgttat taaatcctta ccacctactt
     1381 acgtctcaca gacagcctct ataaaacaag tctgcagcac agaaggcagc atggaggcca
     1441 tgcagtgcca tcaggggcct aagaactgca ggcttcaaag gtcaatgccc cctgcaatga
     1501 atgggtttct gttacaatgt atttcatata tatgaagtta tttcccccaa attttatact
     1561 tactttgtat ctatgtgcat gtggaagtca gaggataact tgtggaatta attctccttt
     1621 ccaccatgtg ggtcctgggg gactcaggtc ctcagcatta gtgggagaca tttttaccca
     1681 gtgaaccatc tctccagccc aacacaaatc atttttgttt acacatgtga ataaacaaaa
     1741 cagaacag
//
