LOCUS       NM_013700               3160 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus ubiquitin specific peptidase 5 (isopeptidase T)
            (Usp5), transcript variant 1, mRNA.
ACCESSION   NM_013700
VERSION     NM_013700.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Eibach,Y., Kreher,S., Poetsch,M.S., Kho,A.L., Gaertner,U.,
            Clemen,C.S., Schroder,R., Guo,K., Milting,H., Meder,B., Potente,M.,
            Richter,M., Schneider,A., Meiners,S., Gautel,M. and Braun,T.
  TITLE     The deubiquitinase USP5 prevents accumulation of protein aggregates
            in cardiomyocytes
  JOURNAL   Sci Adv 11 (4), eado3852 (2025)
   PUBMED   39841822
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Yang,J.H., Gao,J., E,Y.Q., Jiao,L.J., Wu,R., Yan,Q.Y., Wei,Z.Y.,
            Yan,G.L., Liang,J.L. and Li,H.Z.
  TITLE     Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating
            autophagy through promoting deubiquitination of adenosine
            5'-monophosphate (AMP)-activated protein kinase alpha1 via
            ubiquitin specific peptidase 5
  JOURNAL   J Cachexia Sarcopenia Muscle 15 (5), 2118-2133 (2024)
   PUBMED   39189428
  REMARK    GeneRIF: Hydrogen sulfide inhibits skeletal muscle ageing by
            up-regulating autophagy through promoting deubiquitination of
            adenosine 5'-monophosphate (AMP)-activated protein kinase alpha1
            via ubiquitin specific peptidase 5.
REFERENCE   3  (bases 1 to 3160)
  AUTHORS   He,J., Chen,Y., Xu,M. and Wu,S.
  TITLE     USP5 negatively regulates the activation of NLRP3 inflammasomes and
            participates in the pathological and physiological processes of
            Sjogren's syndrome
  JOURNAL   Int Immunopharmacol 135, 112274 (2024)
   PUBMED   38772301
  REMARK    GeneRIF: USP5 negatively regulates the activation of NLRP3
            inflammasomes and participates in the pathological and
            physiological processes of Sjogren's syndrome.
REFERENCE   4  (bases 1 to 3160)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3160)
  AUTHORS   Xiao,X., Shi,J., He,C., Bu,X., Sun,Y., Gao,M., Xiang,B., Xiong,W.,
            Dai,P., Mao,Q., Xing,X., Yao,Y., Yu,H., Xu,G., Li,S., Ren,Y.,
            Chen,B., Jiang,C., Meng,G., Lee,Y.R., Wei,W., Freeman,G.J., Xie,C.
            and Zhang,J.
  TITLE     ERK and USP5 govern PD-1 homeostasis via deubiquitination to
            modulate tumor immunotherapy
  JOURNAL   Nat Commun 14 (1), 2859 (2023)
   PUBMED   37208329
  REMARK    GeneRIF: ERK and USP5 govern PD-1 homeostasis via deubiquitination
            to modulate tumor immunotherapy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3160)
  AUTHORS   Tian,L., Wu,X., Lin,Y., Liu,Z., Xiong,F., Han,Z., Zhou,Y., Zeng,Q.,
            Wang,Y., Deng,J. and Chen,H.
  TITLE     Characterization and potential function of a novel pre-implantation
            embryo-specific RING finger protein: TRIML1
  JOURNAL   Mol Reprod Dev 76 (7), 656-664 (2009)
   PUBMED   19156909
REFERENCE   7  (bases 1 to 3160)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3160)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   9  (bases 1 to 3160)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3160)
  AUTHORS   Ansari-Lari,M.A., Oeltjen,J.C., Schwartz,S., Zhang,Z., Muzny,D.M.,
            Lu,J., Gorrell,J.H., Chinault,A.C., Belmont,J.W., Miller,W. and
            Gibbs,R.A.
  TITLE     Comparative sequence analysis of a gene-rich cluster at human
            chromosome 12p13 and its syntenic region in mouse chromosome 6
  JOURNAL   Genome Res 8 (1), 29-40 (1998)
   PUBMED   9445485
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142254.4.
            
            On Aug 10, 2022 this sequence version replaced NM_013700.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066993.1, ERR2680377.586392.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC142254.4         104603-104759
            158-283             AC142254.4         107620-107745
            284-350             AC142254.4         108866-108932
            351-484             AC142254.4         109059-109192
            485-630             AC142254.4         109343-109488
            631-815             AC142254.4         109728-109912
            816-910             AC142254.4         110497-110591
            911-1104            AC142254.4         111325-111518
            1105-1176           AC142254.4         112087-112158
            1177-1264           AC142254.4         112636-112723
            1265-1390           AC142254.4         112842-112967
            1391-1544           AC142254.4         113479-113632
            1545-1719           AC142254.4         113883-114057
            1720-1808           AC142254.4         114583-114671
            1809-2000           AC142254.4         115378-115569
            2001-2144           AC142254.4         116013-116156
            2145-2290           AC142254.4         116232-116377
            2291-2444           AC142254.4         116573-116726
            2445-2529           AC142254.4         116976-117060
            2530-3160           AC142254.4         118385-119015
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.17 cM"
     gene            1..3160
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /note="ubiquitin specific peptidase 5 (isopeptidase T)"
                     /db_xref="GeneID:22225"
                     /db_xref="MGI:MGI:1347343"
     exon            1..157
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..2623
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific protease 5; ubiquitin carboxyl-terminal
                     hydrolase 5; isopeptidase T; ubiquitin thioesterase 5;
                     deubiquitinating enzyme 5; ubiquitin-specific-processing
                     protease 5; ubiquitin thiolesterase 5; ubiquitin carboxy
                     terminal hydrolase T"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 5 isoform
                     1"
                     /protein_id="NP_038728.1"
                     /db_xref="CCDS:CCDS20531.1"
                     /db_xref="GeneID:22225"
                     /db_xref="MGI:MGI:1347343"
                     /translation="MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLY
                     ICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIG
                     VEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSA
                     SRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSIL
                     CGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHL
                     SHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNS
                     CYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKP
                     ALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERN
                     CRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEK
                     KRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFP
                     DYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTP
                     DEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGN
                     SGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQ
                     ALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGP
                     GKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRV
                     VS"
     exon            158..283
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            284..350
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            351..484
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            485..630
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            631..815
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            816..910
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1104
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1176
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1264
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1390
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1544
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1719
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1808
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..2000
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2144
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2290
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2444
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2529
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..3160
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3151..3156
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /note="hexamer: AATAAA"
     polyA_site      3160
                     /gene="Usp5"
                     /gene_synonym="ISOT; ISOT-1; Ucht"
                     /note="major polyA site"
ORIGIN      
        1 aggtacagtg ggagctgctg tgtgaggagg agctgctgcc ggtgtcatgg cggagctgag
       61 tgaagaggcg ctgctgtcag tgttaccgac gatccgtgtc cccaaggcgg gagaccgggt
      121 ccataaagac gagtgcgctt tctctttcga cacgccggag tctgagggtg gcctctatat
      181 ctgcatgaac acattcctgg gattcgggaa gcagtatgtg gagagacact tcaacaagac
      241 aggccagcgt gtctacctgc acctccggag gacccggcga ccgaaagaag aggacaccag
      301 tgcaggcact ggagacccac ctcggaagaa gcccacccgg ctggccattg gtgttgaagg
      361 agggtttgac ctcaccgagg acaagtttga atttgacgag gatgtgaaga ttgtcatttt
      421 gcccgattac ctggagatcg ctcgggatgg gttggggggg cttcctgaca ttgtcagaga
      481 tcgggtgacc agtgctgtgg aagccctact ttctgctgac tcagcctctc gaaagcagga
      541 ggtccaggct tgggatgggg aagtccgtca ggtgtccaag catgccttca acctcaagca
      601 gttggacaac cctgctcgga tccctccctg tggctggaag tgctccaagt gcgacatgcg
      661 cgagaacctg tggctcaacc tgacagacgg ctccatcctc tgtggacggc gctacttcga
      721 tggcagtggg ggcaacaacc atgctgtgga gcactacagg gagactggct accccttagc
      781 tgttaagctg ggcaccatca ccccggatgg agctgacgtg tattcttacg acgaggatga
      841 catggttctg gaccccagcc tggcagagca cttgtctcac tttggcatcg acatgctgaa
      901 gatgcagaag acggacaaga cgatgactga gctggagata gacatgaacc agcggattgg
      961 tgaatgggag ttgatccagg agtcaggcgt gccactcaag cctctgtttg ggcctggtta
     1021 cacgggcatc cggaacctgg gtaacagctg ctatctcaac tccgtggtcc aggtgctctt
     1081 cagcatccct gactttcaga ggaagtatgt ggataaattg gagaagatct tccagaatgc
     1141 cccaacggac cccacccaag acttcagcac ccaggtggcc aagctggggc atggccttct
     1201 ctctggagaa tactctaagc cagcactgga gtcaggtgat ggggagcagg taccagaaca
     1261 aaaggaagtt caagatggca ttgcccctcg aatgttcaag gccctcattg gcaagggcca
     1321 ccctgagttc tccactaatc ggcagcagga tgcccaggaa ttcttcctac atcttatcaa
     1381 catggtggag aggaattgcc ggagttctga aaatccaaat gaagtgttcc gctttttggt
     1441 ggaagaaaag atcaaatgcc tggccacaga gaaggttaag tacactcagc gagttgacta
     1501 catcatgcag ttgcctgtac ccatggatgc agcccttaac aaagaggagc ttttagagta
     1561 tgaagagaag aagcggcaag ctgaagagga gaaagtgcca ctgccagaac tggttcgggc
     1621 ccaggtgccc ttcagctcct gcctggaagc ctacggggcc cctgagcagg tggacgactt
     1681 ctggagtaca gctctacagg cgaagtcggt cgctgtcaag accacgcgct ttgcctcctt
     1741 ccccgactac ctggtcatcc agatcaagaa gttcaccttt ggcttagact gggtgcccaa
     1801 gaagctggat gtgtccattg agatgccaga ggagctcgat atctcccagc tgaggggcac
     1861 agggctacag ccgggagagg aggagctgcc tgacattgcc ccacccctgg tcactccgga
     1921 tgagcccaaa ggtagccttg gtttctatgg caacgaagac gaagactcct tctgctcccc
     1981 tcacttctcc tctccgacat cacccatgct ggacgaatcc gtcatcatac agttggtgga
     2041 aatgggcttc cctatggatg cctgccggaa agctgtgtac tatacgggaa acagcggggc
     2101 cgaagcagcc atgaactggg tcatgtcaca catggatgat ccagattttg caaaccccct
     2161 catcctgcct ggctccagtg ggcctggctc cacaagtgca gcagctgacc ccccgccgga
     2221 ggactgtgtg accaccatag tctccatggg cttctccagg gaccaggccc tgaaagctct
     2281 gcgggccacg aacaatagct tagagcgggc tgtagactgg atcttcagcc acattgatga
     2341 cctggacgca gaagctgcta tggacatctc agagggacgc tcagcggctg agtccatctc
     2401 cgagtctgtg ccagtgggac ctaaagtccg ggatggtcct ggaaagtatc agctctttgc
     2461 cttcattagt cacatgggca catctactat gtgtggtcac tatgtctgcc atatcaagaa
     2521 ggaaggcagg tgggtgatct acaatgacca gaaagtgtgt gcctctgaaa agccacccaa
     2581 ggacctgggc tacatctact tctaccagag agtggtcagc taagagcctg ccctcagccc
     2641 ttccacttga agcaggggac agaccatctg gcatgaggga cggctgaggg atggacttca
     2701 gcccctgctc tgtacccttt ttgtttttgt ccccagcagt gggaaggagc tggaggtcat
     2761 aggagaatgg ccaaacaaag aggaggggta ctcattagac tctggggaca gagcagggat
     2821 gggacaggag gggccaactg cctgtccaca gtgggacctt gttgcttcct tcccctgccc
     2881 cttgaatcca cagtgctctg cttctctgtg atggcccaac ccctgatgtg gggcgggctc
     2941 ttacctgtgt gtgtgtgtgg atgtggcttt atgcatcttt ccaggggagg gagcgactgc
     3001 gcctcccctc cccttaagtg tttgctccct tgtggtgggg cagaggaggc tctgagggaa
     3061 ataggaccct ccccacaccc tttgcccttc tgcctcagcc tccggtctgt ctttatggtc
     3121 ctctggaaaa atgccaaaat acatgatgtg aataaaagta
//
