LOCUS       NM_001290425            5874 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ubiquitin specific petidase 45 (Usp45), transcript
            variant 2, mRNA.
ACCESSION   NM_001290425 XM_006538363
VERSION     NM_001290425.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Yi,Z., Ouyang,J., Sun,W., Xiao,X., Li,S., Jia,X., Wang,P. and
            Zhang,Q.
  TITLE     Biallelic mutations in USP45, encoding a deubiquitinating enzyme,
            are associated with Leber congenital amaurosis
  JOURNAL   J Med Genet 56 (5), 325-331 (2019)
   PUBMED   30573563
REFERENCE   3  (bases 1 to 5874)
  AUTHORS   Perez-Oliva,A.B., Lachaud,C., Szyniarowski,P., Munoz,I.,
            Macartney,T., Hickson,I., Rouse,J. and Alessi,D.R.
  TITLE     USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA
            damage responses
  JOURNAL   EMBO J 34 (3), 326-343 (2015)
   PUBMED   25538220
  REMARK    GeneRIF: these results establish USP45 as a new regulator of
            XPF-ERCC1 crucial for efficient DNA repair
REFERENCE   4  (bases 1 to 5874)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   5  (bases 1 to 5874)
  AUTHORS   Kambouris,M., Triarhou,L.C., Dlouhy,S.R., Sangameswaran,L., Luo,F.,
            Ghetti,B. and Hodes,M.E.
  TITLE     Novel cDNA clones obtained by antibody screening of a mouse
            cerebellar cDNA expression library
  JOURNAL   Brain Res Mol Brain Res 25 (3-4), 183-191 (1994)
   PUBMED   7808216
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB213271.2, AK019756.1, AL772187.6 and AI465456.1.
            
            On Mar 12, 2014 this sequence version replaced XM_006538363.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. It encodes
            isoform 2, which is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.4566.1, AK088073.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                BB213271.2         13-60
            49-331              AK019756.1         91-373
            332-1225            AK019756.1         447-1340
            1226-2040           AK019756.1         1485-2299
            2041-2362           AK019756.1         2301-2622
            2363-5854           AL772187.6         117441-120932
            5855-5874           AI465456.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 8.93 cM"
     gene            1..5874
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /note="ubiquitin specific petidase 45"
                     /db_xref="GeneID:77593"
                     /db_xref="MGI:MGI:101850"
     exon            1..48
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            49..158
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..2356
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /EC_number="3.4.19.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     granule cell antiserum positive 7; ubiquitin specific
                     protease 45; ubiquitin carboxyl-terminal hydrolase 45;
                     ubiquitin thioesterase 45; deubiquitinating enzyme 45;
                     ubiquitin-specific-processing protease 45; ubiquitin
                     thiolesterase 45"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 45 isoform
                     2"
                     /protein_id="NP_001277354.1"
                     /db_xref="CCDS:CCDS71351.1"
                     /db_xref="GeneID:77593"
                     /db_xref="MGI:MGI:101850"
                     /translation="MRVKDPSKDLPEKGKRNKRPLLPHDEDSSDDIAVGLTCQHVSYA
                     VSVNHVKKAVAESLWSVCSECLKERRFCDGQPVLPADVWLCLKCGLQGCGKNSESQHS
                     LRHFKSSGTESHCVVISLSTWVIWCYECNEKLSTHCNKKVLAQIVDFLQKHAFKTQTG
                     AFSRIIKLCEEKREAGEIKKGKKGCTVPSVKGITNLGNTCFFNAVIQNLAQTYILFEL
                     MNEIKEDGTKFKISLSSAPQLEPLVVELSSPGPLTSALFLFLHSMKEAEKGPLSPKVL
                     FNQLCQKAPRFKGFQQQDSQELLHHLLDAVRTEETKRIQASILKAFNNPTTKTADDET
                     RKKVKAYGKEGVKMNFIDRIFIGELTSTVMCEECANISTMKDPFIDISLPIIEERNQL
                     SHDRKHLRKWPSEEEKTVVTHPKNDNLEASPPASTLSTEASLNESLTDGSERDASLES
                     SVDADSEASEPEIASKQPVLLRSRGDSCGHAEQHPHLPLASELPQAKETHGGEEEMAE
                     AIAELHLSGTVTGNRDFHREKQPLNVPNNLCFSEGKHTRLHSAQNAFQTLSQSYVTTS
                     KECSVQSCLYQFTSMELLMGNNKLLCEDCTEKRRKCHKETSSAEKKAGGVYTNARKQL
                     LISAVPAILILHLKRFHQAGLSLRKVNRHVDFPLTLDLAPFCAATCKNISVGEKVLYG
                     LYGIVEHSGSMRGGHYTAYVKVRVPSRKLSECITGRKTAAGLKEPDGELGGHWVHVSD
                     TYVQVVPESRALSAQAYLLFYERIL"
     exon            159..331
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            332..435
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            436..536
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            537..679
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            680..775
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            776..906
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            907..994
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1076
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1168
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1225
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1886
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1984
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2071
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2225
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..5863
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5769..5774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /note="hexamer: AATAAA"
     polyA_site      5793
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /note="major polyA site"
     regulatory      5841..5846
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /note="hexamer: AATAAA"
     polyA_site      5863
                     /gene="Usp45"
                     /gene_synonym="3110003C05Rik; 4930550B20Rik; Gcap7"
                     /note="major polyA site"
ORIGIN      
        1 acaggcgcag aagctcctgc gtgtggaagc tgcggcccgg cgggcgggta aataacagat
       61 gcgggtaaaa gatccatcaa aagatctacc tgaaaaaggc aaaagaaata aaagaccact
      121 gctgcctcat gatgaagatt cttccgatga cattgctgta ggtctaacgt gccagcacgt
      181 cagttatgct gtcagtgtga atcatgtgaa gaaagcagta gctgagagtc tgtggtcagt
      241 ttgttcagaa tgtttgaaag agagaagatt ctgcgatgga cagccagtac tgcctgctga
      301 tgtttggttg tgcctcaagt gtggtttgca gggatgcggt aaaaactcag aaagccagca
      361 ttcactgaga cacttcaaga gctctgggac agagtcccat tgtgttgtca tcagcctgag
      421 tacatgggtc atatggtgtt atgaatgcaa tgaaaagttg tcgacacatt gcaacaagaa
      481 agttttggct caaatagttg attttctcca gaaacatgct tttaaaacac aaacaggtgc
      541 attttctaga atcataaaac tttgtgaaga aaaacgtgaa gcaggtgaaa taaagaaggg
      601 gaagaaaggc tgcactgtgc catctgtcaa aggaattaca aacttaggga atacttgctt
      661 ttttaatgca gtcattcaga acttggctca gacttacatt ctttttgaac tgatgaacga
      721 gataaaagaa gatggcacca aattcaagat ttctctttcg tcagctccac agctggagcc
      781 gttagtggtg gaactctcga gcccaggacc actgacgtca gccttgttcc tgtttcttca
      841 cagcatgaag gaggctgaaa aaggaccact ttctcctaaa gttcttttca atcagctttg
      901 tcagaaggct cctcggttta aaggtttcca gcaacaggac agccaggagc ttctgcacca
      961 tctgttggat gcagtgagga cagaggagac caagaggata caagccagca ttctaaaagc
     1021 cttcaacaac ccaactacca agactgctga tgatgaaact agaaaaaagg tcaaagccta
     1081 tggaaaggaa ggtgtgaaga tgaacttcat agatcgcatc tttattggcg aattaactag
     1141 cacagtcatg tgtgaagaat gtgcgaacat ctctaccatg aaggatccct tcattgatat
     1201 ctcacttcct atcatagaag aaaggaacca actaagtcat gacagaaaac atttaagaaa
     1261 atggccatct gaagaagaga aaactgttgt tacacaccca aagaatgaca acttggaagc
     1321 cagcccgcct gccagcaccc tcagcactga ggcgtccctg aacgagagcc tcactgacgg
     1381 cagcgagagg gacgccagcc tagagagcag cgtggatgcc gacagcgagg cttcggagcc
     1441 cgagatcgct tccaagcagc ctgtgctgct gcggtccagg ggtgattcct gcggacacgc
     1501 ggagcagcac ccacacctcc cactggcaag tgaattgcca caagccaagg agacgcatgg
     1561 tggcgaggag gaaatggcgg aagcgattgc tgaacttcat ttgagtggca ctgtaactgg
     1621 caatagagat ttccacaggg aaaaacagcc actaaatgtc ccaaataatt tgtgtttttc
     1681 agagggaaaa catacgaggc ttcacagtgc ccaaaatgct tttcagaccc tttctcagag
     1741 ttatgtaact acttctaaag aatgctcagt ccagtcctgt ctctaccagt tcacatccat
     1801 ggagttatta atgggcaaca ataagctgct ctgtgaggac tgtactgaaa agagacggaa
     1861 gtgccacaaa gaaaccagct ccgcagaaaa gaaagcagga ggagtttata ccaatgccag
     1921 gaagcaactc ctcatatctg ccgttcctgc tatcctaatc cttcatctta aaagattcca
     1981 ccaggctggc ttgagtcttc gtaaagtaaa cagacatgta gactttccgc ttacccttga
     2041 cttggcaccg ttctgtgcgg ctacctgtaa gaatataagt gtgggggaga aggttctcta
     2101 cggtctctac ggtatagtgg agcacagcgg ctccatgaga ggaggccact acactgctta
     2161 cgtgaaagtg agagtaccct ccaggaagct gtccgagtgc atcaccggga ggaagactgc
     2221 agccggtttg aaagaacctg atggtgaatt gggaggccac tgggtccatg tcagcgacac
     2281 ctatgtgcag gtggttccag agtcaagagc cctgagtgca caagcttatc ttctttttta
     2341 tgaaagaata ttataatatc tgttcattgt tatttagttc atttttattt taatgctgaa
     2401 gtcgtaacca taatatgtaa tgtgccttct tagtctttag gaatagtgca tccttccagc
     2461 gttatttaac tctttcaaca tgtggtgaat cctttaaaaa aaaattaatt aatttgacag
     2521 gcagttgtgc atgcctttaa ttccagcact tgggaggcag aggcagacag atctctgttt
     2581 gaggccaact agggctattc aataaaacct tgtcttgaaa tattaaacaa aaagttaatt
     2641 aatgctttca aatctatatt cttttttttt cttttttttt gcttttgtga gacagggttt
     2701 ctctgtgtag ccctggctgt cctggaactc actttgtatc actttgtaga ccaggctggc
     2761 ctcgaactca gaaatccacc tacctctgcc tcccaagtgc tggcattaaa ggcgtgcgcc
     2821 accactgcct ggctcaaatc tatattttta aatgtaaaaa catgttttat gttaattttc
     2881 atgtccatgg aaattgcccc tacacacaca cacacagagt tgatagtaaa gcaaccccct
     2941 ctatgatatg actcatcctt acatgcattc aaagatgatg ctaaattgga tctttagtat
     3001 actatttcct aatgtaatac aaccttcctg agtggatcca atatatctta tgctacaatt
     3061 tgcttacttt ttgttgaagc cttttttaag cttggctgtt ttgaatcata cttcacattt
     3121 ttatgtgcta gttatttttc tagtaaacat tgatgtgagt ttataactat gaaaggaaaa
     3181 tcatacatcc ttgcaccacc ccactttaaa tcatctcagg gctctgcagt ggtgtgtgtg
     3241 ttagtctata ggaaaagttt ctagaacagt ctgaactagt gcctaatgta acataattaa
     3301 tttttcatat tttatgtgag tcagtagtat aaaaaggagc cattgtaatt ttggtagtgt
     3361 gagtttacaa gtaatgaatt gattcgctta ttgctttgaa tatttgaagg tattgaaaca
     3421 tttcataaac taatcatttg cagtctttga aagataattt acaacacata aaactggtaa
     3481 atagaagtta tagtctatat aggaatatag aatactttaa agtttaagga tatctttgaa
     3541 ctgtaaacca ccaaattata tttataaatg gtagagatca tattcttata accaacaatg
     3601 aatatataat acttttttac ttctatacat taatacatgt taagcatagt tttgtcttca
     3661 taagcattta tttaaaactt caggtggata gttagggttg tagttcaatg gcaaagcact
     3721 tgcctggttc catccctagc aaaacaaaga aaatattcag gtgggcttgg ctactttcat
     3781 gggacacaga cttgccactt ctgttcccct taaataagca gtaacacaga atagtttctt
     3841 ctagcattca aaggattgaa ctgtgaaaac ttagcatgaa aaatattact accaagattt
     3901 tcaataatca atgaagtggc tcactgatac atagtattga atcaagatgt aagggaacag
     3961 gttcaatgtc ttagtcatca gtgcctgtgt ttgattctat gggcaaatgt gagggagaga
     4021 ggctgtaaac tgtccagacc tttagccagg atggaacctt cttaacatgt gtaacataaa
     4081 gtccccagtg ttgcctcttg atatgtggct gggtgaaact gattgtctaa tggagacatt
     4141 ttaagattgt ggagaaattt tcaatggtat ttgaattttt ttaattgaag gtgaagaaat
     4201 atattttatc tgtagagccc agttgtgtcc tctaaaaaga actttccagt gtcttctgtt
     4261 tcatcagaca ttcagtgtta ttaatctatt gacacggaag agtctagcac cagagcaatg
     4321 aatgagaaaa ataatttatt tgttcaagct agcctgtcat agcagttgca cccacacccc
     4381 caaacaaatg caagcggtca gcctttttcc cccctctctc ctggaaaaga tactattaag
     4441 acatgtacaa tctaagcagg ttccaatacc gggcatatga tagtatttta tgagccttta
     4501 aaacagaaaa acctgctgag tgatggtggc ccatgccttt agattcagca cttgggaggc
     4561 agaggcaggt ggatttgagt tcaaggacac cctggtccac agagcaggtt ccaggaaagc
     4621 caagacaaca cagagaaacc ttgcctgaaa aaccttgggg aaaaaaaaaa caccctaaaa
     4681 tctatatgat tttatacatt attctagtag tgatgtacaa ataaaatgtt catttattac
     4741 ctagttctga ggagattctg gaatgtattt ttttttaatg acctaaagtt tgagtgtttt
     4801 tcttgtcacc tgaaaatgta ggaaaatggt ttgatttgat atctaggaat ttcttatcaa
     4861 gcttatgtaa tatagaaaaa tcacccagat ctacacttaa atttttgttc ttactttgaa
     4921 gttttccaaa gcacatttgt agaaatttta aattgtgagg ccactgtact aacagattac
     4981 ctcaggaaac cgtgcaaaca aacaagaatg tttacagatc cgcacaacta gtcagtgctt
     5041 tggcaccgtg aatttgaaag gtaaaagaga gaagttttta tctagaaact ggtttacagg
     5101 gtacctctag ccccagttct cccctgactt tcccatattt aaaataataa aattctgatg
     5161 atgtgccaat tatgtgaatg cttgtgacat aaacaaatat acatctgtat ttaagatttg
     5221 ttgtttaatg ttttaaaaaa taatatgaag actgcttatg taggtggtca aggggatgat
     5281 catatatcaa cagagctatt ttaccatagt tcttagcttg catgtgtgat taattagtga
     5341 tttttatgta atacttgaat aagatatcct gaagatatga aatgtcttcg tgaaatgtag
     5401 ctgtgaaatg tcaccgcaca tcaaattttt atagtacaaa ataacatcct gcctactttc
     5461 atgaacttta tactcagctt atttttctgc atgacatctg gtcatataaa cctaaaccaa
     5521 atatgtgcga taaaatcaag ctttcagcta gcatccattg cagtattaga gaaatgattt
     5581 ctgcattaaa atgtttttgt tcatgcagaa atcatcaatt gaaatgtgtt taaaagatga
     5641 ttatttttag gtatcagtaa tacattcatg gtatcattcc ttctctttca tccagtttta
     5701 cagtatttta aaaatatctg tgtaacttat tgagcattat ttgatcattg ctgtatgtaa
     5761 aagatcacaa taaatattag attcttatgt taattgatta ggcctagagt ttttgcatta
     5821 tacattgtta gcagaatgtt aataaacatt gtgtcattgc ctaaaaaaaa aaaa
//
