LOCUS       NM_001376981            4013 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus ubiquitin specific peptidase 44 (Usp44), transcript
            variant 1, mRNA.
ACCESSION   NM_001376981 XM_011243479
VERSION     NM_001376981.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Liu,Y., Yuan,M., Xu,X., Yang,H., Yao,Y., Hou,P., Yu,W. and Ji,M.
  TITLE     USP44 inactivation accelerates the progression of thyroid cancer by
            inducing ubiquitylation and degradation of p21
  JOURNAL   Int J Biol Sci 20 (13), 5223-5238 (2024)
   PUBMED   39430240
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4013)
  AUTHORS   Zhang,Y., Mandemaker,I.K., Matsumoto,S., Foreman,O., Holland,C.P.,
            Lloyd,W.R., Sugasawa,K., Vermeulen,W., Marteijn,J.A. and
            Galardy,P.J.
  TITLE     USP44 Stabilizes DDB2 to Facilitate Nucleotide Excision Repair and
            Prevent Tumors
  JOURNAL   Front Cell Dev Biol 9, 663411 (2021)
   PUBMED   33937266
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4013)
  AUTHORS   Yang,J., Wei,P., Barbi,J., Huang,Q., Yang,E., Bai,Y., Nie,J.,
            Gao,Y., Tao,J., Lu,Y., Xie,C., Hou,X., Ren,J., Wu,X., Meng,J.,
            Zhang,Y., Fu,J., Kou,W., Gao,Y., Chen,Z., Liang,R., Tsun,A., Li,D.,
            Guo,W., Zhang,S., Zheng,S.G., Niu,J., Galardy,P., Tong,X., Shi,G.,
            Li,H., Pan,F. and Li,B.
  TITLE     The deubiquitinase USP44 promotes Treg function during inflammation
            by preventing FOXP3 degradation
  JOURNAL   EMBO Rep 21 (9), e50308 (2020)
   PUBMED   32644293
REFERENCE   5  (bases 1 to 4013)
  AUTHORS   Lin,Y.H., Forster,M., Liang,Y., Yu,M., Wang,H., Robert,F.,
            Langlais,D., Pelletier,J., Clare,S. and Nijnik,A.
  TITLE     USP44 is dispensable for normal hematopoietic stem cell function,
            lymphocyte development, and B-cell-mediated immune response in a
            mouse model
  JOURNAL   Exp Hematol 72, 1-8 (2019)
   PUBMED   30639577
  REMARK    GeneRIF: USP44 is dispensable for the normal physiology of
            hematopoietic and immune systems, and its functions in these
            systems are likely redundant with other USP family proteins.
REFERENCE   6  (bases 1 to 4013)
  AUTHORS   Jahan,A.S., Lestra,M., Swee,L.K., Fan,Y., Lamers,M.M.,
            Tafesse,F.G., Theile,C.S., Spooner,E., Bruzzone,R., Ploegh,H.L. and
            Sanyal,S.
  TITLE     Usp12 stabilizes the T-cell receptor complex at the cell surface
            during signaling
  JOURNAL   Proc Natl Acad Sci U S A 113 (6), E705-E714 (2016)
   PUBMED   26811477
REFERENCE   7  (bases 1 to 4013)
  AUTHORS   Zhang,Y., Foreman,O., Wigle,D.A., Kosari,F., Vasmatzis,G.,
            Salisbury,J.L., van Deursen,J. and Galardy,P.J.
  TITLE     USP44 regulates centrosome positioning to prevent aneuploidy and
            suppress tumorigenesis
  JOURNAL   J Clin Invest 122 (12), 4362-4374 (2012)
   PUBMED   23187126
  REMARK    GeneRIF: USP44 inhibited chromosome segregation errors independent
            of its role in the mitotic checkpoint by regulating centrosome
            separation, positioning, and mitotic spindle geometry.
REFERENCE   8  (bases 1 to 4013)
  AUTHORS   Fuchs,G., Shema,E., Vesterman,R., Kotler,E., Wolchinsky,Z.,
            Wilder,S., Golomb,L., Pribluda,A., Zhang,F., Haj-Yahya,M.,
            Feldmesser,E., Brik,A., Yu,X., Hanna,J., Aberdam,D., Domany,E. and
            Oren,M.
  TITLE     RNF20 and USP44 regulate stem cell differentiation by modulating
            H2B monoubiquitylation
  JOURNAL   Mol Cell 46 (5), 662-673 (2012)
   PUBMED   22681888
  REMARK    Erratum:[Mol Cell. 2015 Oct 15;60(2):338]
REFERENCE   9  (bases 1 to 4013)
  AUTHORS   Zhang,Y., van Deursen,J. and Galardy,P.J.
  TITLE     Overexpression of ubiquitin specific protease 44 (USP44) induces
            chromosomal instability and is frequently observed in human T-cell
            leukemia
  JOURNAL   PLoS One 6 (8), e23389 (2011)
   PUBMED   21853124
  REMARK    GeneRIF: These data are consistent with an important role for USP44
            in regulating Cdc20-APC/C activity and suggest that high levels of
            this enzyme may contribute to the pathogenesis of T-cell leukemias.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124686.3.
            
            On Dec 6, 2019 this sequence version replaced XM_011243479.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.174403.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AC124686.3         74423-74504
            83-143              AC124686.3         78574-78634
            144-227             AC124686.3         81943-82026
            228-301             AC124686.3         98421-98494
            302-1805            AC124686.3         100054-101557
            1806-2001           AC124686.3         104593-104788
            2002-2110           AC124686.3         107153-107261
            2111-2316           AC124686.3         110557-110762
            2317-4013           AC124686.3         112172-113868
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 48.67 cM"
     gene            1..4013
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /note="ubiquitin specific peptidase 44"
                     /db_xref="GeneID:327799"
                     /db_xref="MGI:MGI:3045318"
     exon            1..82
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            83..143
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            144..227
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            228..301
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            302..1805
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..2516
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /EC_number="3.4.19.12"
                     /note="inactive ubiquitin carboxyl-terminal hydrolase 44;
                     inactive ubiquitin thioesterase 44; inactive
                     deubiquitinating enzyme 44; inactive
                     ubiquitin-specific-processing protease 44; ubiquitin
                     specific peptidase 49 pseudogene"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 44"
                     /protein_id="NP_001363910.1"
                     /db_xref="GeneID:327799"
                     /db_xref="MGI:MGI:3045318"
                     /translation="MDRCKHVEQLQLAQGHSILDPQKWYCMVCNTTESIWACLSCSHV
                     ACGKYIQEHALKHFQESSHPVAFEVNDMYAFCYLCNDYVLNDNAAGDLKSLRSTLSTI
                     KSKKYPCVVPSDSVLHPVDAQDRVYSLLDGTQSLPGNEDPTCAALWHRRRVLMGKAFR
                     TWFEQSAIGRKGQEPTQERMVAKREAKRRQQQELEQQMKAELESTPPRKSLRLQGSSE
                     EAATIEIVPVRAPPPPPASPAKDKAALPTSEDRTFKKVSDSLIKRRPMVTPGVTGLRN
                     LGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAVAASDKARSYKHSAVTEAAAQQMN
                     EGQEKEKGFVCSRHSGLSSGLSGGASKGRNMELIQPREPSSPYSSLCHELHILFQVMW
                     SGEWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTKLPALIP
                     TSQRRLIEQVLNVVNNIFHGQFLSQVTCLACDNKSDTIESFWDLSLEFPERYQCSGKD
                     AASQPCLVTDMLDKFTETEALEGKIYMCDHCNSKRRKFSSKSVVFTEAQKQLMICHLP
                     QVLRLHLKRFRWSGRNNREKIGVHVVFEETLNMEPYCCRETLNALRPECFLYNLSAVV
                     IHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMEEVRKAQAYILFYTQRVTENG
                     HSKLLPPELLSNSQHPSKETDASSNEVLS"
     exon            1806..2001
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2110
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2316
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..4013
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2572
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /note="major polyA site"
     regulatory      2713..2718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      2731
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
     regulatory      3988..3993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4013
                     /gene="Usp44"
                     /gene_synonym="E430004F17Rik"
                     /note="major polyA site"
ORIGIN      
        1 agacgaaaat ggaaagcttt ggatgaaggg agcctgccct ggtgagaagt ctgaggactc
       61 agaaaggctg gtatgaggac aatggcttca acatgatcca ctcagctcaa ttttaaaccg
      121 atgctgtcac ctggtaaact ctgtgctagg atcaagggtg tgcgctgctg tatccagttt
      181 taaacaggat tgaacatagt acctccacag gacctggcgc ttagcagaat tctgtgctga
      241 ccttatattg cccatagaca agttgaggag tgatgcagac cgagaatcct ccaagaccca
      301 gttttcagat gctcccggtg ttgtgaagag aggcccgtct ggattatgtg tttcccagac
      361 agactagcta cctgctaaca atggataggt gcaagcacgt tgagcagttg cagcttgctc
      421 aaggccattc catcttggac cctcagaaat ggtactgtat ggtctgcaac acaaccgagt
      481 ccatttgggc ttgcctcagc tgttcccacg tcgcctgtgg gaagtacatc caagagcacg
      541 cactgaagca ctttcaagaa agcagccatc ccgttgcctt cgaggtgaac gacatgtacg
      601 ctttttgtta tctttgtaac gattatgttc taaacgataa cgcagccgga gacctgaagt
      661 cactacggag tacactaagt acaatcaaaa gtaaaaagta cccctgtgtg gttccgagtg
      721 actcggtttt acatcctgtg gatgcacagg accgtgttta ctccttactc gacggcaccc
      781 aatctctgcc cggaaacgag gatccaacgt gcgccgccct ctggcacagg agacgggtgc
      841 tcatggggaa agcctttcga acttggttcg aacagtcagc aattggaagg aaagggcaag
      901 agccaactca ggagagaatg gtagcaaaac gagaggcgaa gagaaggcag cagcaggagt
      961 tggagcagca gatgaaagcc gagctggaaa gcacacctcc gcggaagagt ttacgcttac
     1021 aaggctcctc ggaggaggcg gccacaatcg aaatcgtgcc agtgcgggca ccgcccccac
     1081 ctcccgcatc cccagcaaag gacaaagccg cgctacctac ttcggaagac aggactttta
     1141 aaaaagtaag tgactcctta atcaaacgaa ggcccatggt aactcctggt gtaaccggac
     1201 tgagaaactt aggaaatact tgctatatga attctgttct tcaagtgttg agtcatttac
     1261 tcatttttcg acaatgtttt ttaaagcttg acctgaacca gtggctggct gtggctgcca
     1321 gcgataaggc ccgatcctat aagcactcag ccgtcacgga ggcggcggca cagcagatga
     1381 acgaagggca agagaaagag aaaggcttcg tatgctccag acattcgggt ttatcctcag
     1441 gcctgagcgg aggggcctca aaaggtcgga acatggagct tattcagcca agggagccca
     1501 gttccccgta cagttctctc tgccatgaat tgcatatcct gttccaagtc atgtggtctg
     1561 gagagtgggc cttggtctca ccgtttgcca tgcttcactc cgtgtggagg ctgatccctg
     1621 ctttccgcgg ttacgcccag caggatgctc aggaatttct ttgtgaactt ctggataaga
     1681 tacaacgtga actagagaca accggaacca agttaccagc cctcatcccc acttcccaaa
     1741 ggagacttat tgagcaagtt ctgaatgtgg tgaataacat ttttcacgga caatttctta
     1801 gtcaggttac atgtcttgct tgtgacaaca aatcagatac catagaatcc ttctgggact
     1861 tgtccctgga gtttccagag agataccagt gcagtgggaa agatgctgcc tcccagccgt
     1921 gcctggttac tgacatgttg gacaaattta cagaaacaga agctttagaa gggaaaatct
     1981 acatgtgtga ccattgtaac tcaaagcgta gaaagttttc gtcaaaatca gttgtattca
     2041 cagaagccca gaagcagctt atgatatgcc atctacctca ggttctcaga ctgcacctca
     2101 aacggttcag atggtcagga cgtaataacc gagagaagat tggtgttcat gttgtctttg
     2161 aggaaacctt aaacatggag ccttattgct gcagggagac cctgaacgct ctcagaccag
     2221 agtgctttct ctataattta tctgctgtcg taattcacca tggaaaagga tttggctcag
     2281 gacactacac tgcctactgc tacaattccg aaggagggtt ctgggtccac tgcaacgatt
     2341 ccaagctgag catgtgcacg atggaagaag tacgcaaggc ccaagcttat atcttatttt
     2401 atactcagcg agttactgag aatggacatt cgaaactctt gcctcccgag ctcctgtcca
     2461 atagccagca ccccagtaag gagactgatg cctcttctaa tgaagtcctt agctgatcca
     2521 gacagtgggc tctcgttatc tgtatatatc ctttttaaat gggctgacct acgctgctgt
     2581 gcttcattgt ttttattctg gaattatgat cagtgcctgc tatgtttaga cattgtgtat
     2641 ttaactacca agtttttctt ttcttttttt acagagacaa actataaata tatgtatatc
     2701 aactataggc caactaaact tttttcaaat attttgagtt ttatactttt agtgtttacc
     2761 tcagactgtt acctcttata ttctgatgtc ttaattgcct caaatcatga atttgtgcaa
     2821 tctactaaag aaattcaggt ggcatcatta tatatattat tattatttaa cttctgtagc
     2881 tgttttctat acagattcta actccacggg gaaggagacg ctcgtcacag attgtcttgc
     2941 tgactccagc tcttccccgt tggaggtaaa cagattggaa gtgagatact gaagcatcac
     3001 ctagctccag tcactcctga ggcttctaca gggctcggag tagatttaat gcagatactg
     3061 gtctttcact ccaaaattta ttgcttccat aactgtcctt tattgagcac aaattcaaaa
     3121 tgaaaatagc agatttacac atggtatata cttcaatgaa ctttggaact atgggcaatg
     3181 aaaaaatagt tctaataagg cagtcacaaa attttgaatt tagcaaacca atatattatg
     3241 atacacaaat gtagtttatc ttgagaatga gtttttacgt ttaattttag atatcttaag
     3301 tagttgagat ttatttcttt attttttatt tttagttcaa aggagtaaga caaatgcata
     3361 ggggcaaatg cataggggca gatgtttaag gcagagagaa gacagttcga gcatggcacc
     3421 tgggctgtta catctaagat cttgtttttc tcgaagtacc ctaagcctga ctcttgctag
     3481 gattatgtga ccactgggaa tttctcaggg ccctggcccc tttttaaaaa cactagaagt
     3541 ggtctaaact tgaattaact aaaagcaaga gagattcctt tcgctgtgtt ctaaagaaag
     3601 ctttccaggt cttatggatg gagctctgct gctgccatgg ccatggccat ggccaccgcc
     3661 acccaacctg ctcagaaatg gggctccacc aacccacaga tcccaaggaa cctgtgagcc
     3721 actgggagag gttttttttt cccccttctt cctaaagaaa gtgacttaat ttacagctta
     3781 tatctaagcc aggaagagtt atctttagca cagacttctg aaaggagtgt cgcctggggc
     3841 agcttattaa caaaccgcaa caaaaacaca tcaaccgaag acagaatcct atgtgccaaa
     3901 aatacagctg gagtaatatt tggagtgata ttaggtcact tacccaaagc gtatacaaat
     3961 tgttgcatca gctgttaaat atacctcatt aaagattcat gtgataaaag tta
//
