LOCUS       NM_133247               4209 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript
            variant 1, mRNA.
ACCESSION   NM_133247
VERSION     NM_133247.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Dong,W., Chen,J., Wang,Y., Weng,J., Du,X., Fang,X., Liu,W.,
            Long,T., You,J., Wang,W. and Peng,X.
  TITLE     miR-206 alleviates LPS-induced inflammatory injury in
            cardiomyocytes via directly targeting USP33 to inhibit the
            JAK2/STAT3 signaling pathway
  JOURNAL   Mol Cell Biochem 479 (4), 929-940 (2024)
   PUBMED   37256445
  REMARK    GeneRIF: miR-206 alleviates LPS-induced inflammatory injury in
            cardiomyocytes via directly targeting USP33 to inhibit the
            JAK2/STAT3 signaling pathway.
            Erratum:[Mol Cell Biochem. 2024 Feb;479(2):445. doi:
            10.1007/s11010-023-04803-2.. PMID: 37432634]
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4209)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4209)
  AUTHORS   Cubillos-Rojas,M., Schneider,T., Hadjebi,O., Pedrazza,L., de
            Oliveira,J.R., Langa,F., Guenet,J.L., Duran,J., de Anta,J.M.,
            Alcantara,S., Ruiz,R., Perez-Villegas,E.M., Aguilar-Montilla,F.J.,
            Carrion,A.M., Armengol,J.A., Baple,E., Crosby,A.H., Bartrons,R.,
            Ventura,F. and Rosa,J.L.
  TITLE     The HERC2 ubiquitin ligase is essential for embryonic development
            and regulates motor coordination
  JOURNAL   Oncotarget 7 (35), 56083-56106 (2016)
   PUBMED   27528230
REFERENCE   5  (bases 1 to 4209)
  AUTHORS   Chan,N.C., den Besten,W., Sweredoski,M.J., Hess,S., Deshaies,R.J.
            and Chan,D.C.
  TITLE     Degradation of the deubiquitinating enzyme USP33 is mediated by p97
            and the ubiquitin ligase HERC2
  JOURNAL   J Biol Chem 289 (28), 19789-19798 (2014)
   PUBMED   24855649
  REMARK    GeneRIF: Knockdown or chemical inhibition of p97 causes robust
            accumulation of USP33 due to inhibition of its degradation
REFERENCE   6  (bases 1 to 4209)
  AUTHORS   Yuasa-Kawada,J., Kinoshita-Kawada,M., Wu,G., Rao,Y. and Wu,J.Y.
  TITLE     Midline crossing and Slit responsiveness of commissural axons
            require USP33
  JOURNAL   Nat Neurosci 12 (9), 1087-1089 (2009)
   PUBMED   19684588
  REMARK    GeneRIF: Our results reveal a previously unknown role for USP33 in
            vertebrate commissural axon guidance and in Slit signaling
REFERENCE   7  (bases 1 to 4209)
  AUTHORS   Yuasa-Kawada,J., Kinoshita-Kawada,M., Rao,Y. and Wu,J.Y.
  TITLE     Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling
            in inhibiting breast cancer cell migration
  JOURNAL   Proc Natl Acad Sci U S A 106 (34), 14530-14535 (2009)
   PUBMED   19706539
REFERENCE   8  (bases 1 to 4209)
  AUTHORS   Li,Z., Na,X., Wang,D., Schoen,S.R., Messing,E.M. and Wu,G.
  TITLE     Ubiquitination of a novel deubiquitinating enzyme requires direct
            binding to von Hippel-Lindau tumor suppressor protein
  JOURNAL   J Biol Chem 277 (7), 4656-4662 (2002)
   PUBMED   11739384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087467.1, AK143844.1, BC089315.1 and AA210301.1.
            
            On Nov 16, 2011 this sequence version replaced NM_133247.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF383174.1, SRR1660817.16866.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AK087467.1         2-33
            33-2854             AK143844.1         1-2822
            2855-3909           BC089315.1         2777-3831
            3910-4209           AA210301.1         48-347
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 77.15 cM"
     gene            1..4209
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="ubiquitin specific peptidase 33"
                     /db_xref="GeneID:170822"
                     /db_xref="MGI:MGI:2159711"
     exon            1..162
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            163..294
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..2943
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Vhlh-interacting deubiquitinating enzyme 1; ubiquitin
                     specific protease 33; ubiquitin carboxyl-terminal
                     hydrolase 33; pVHL-interacting deubiquitinating enzyme 1;
                     ubiquitin thioesterase 33; deubiquitinating enzyme 33;
                     VHL-interacting deubiquitinating enzyme 1;
                     ubiquitin-specific-processing protease 33; ubiquitin
                     thiolesterase 33"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 33 isoform
                     1"
                     /protein_id="NP_573510.2"
                     /db_xref="CCDS:CCDS17918.1"
                     /db_xref="GeneID:170822"
                     /db_xref="MGI:MGI:2159711"
                     /translation="MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACL
                     ENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSL
                     PHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIG
                     NTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVV
                     PANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETV
                     EEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKE
                     KVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQ
                     ILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGT
                     VISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQG
                     WIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNG
                     VKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTY
                     DLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKS
                     SEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPP
                     RKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIELEKIEKRRKTELEIF
                     IRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGSVMLK
                     QGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL"
     exon            295..348
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            349..411
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            412..564
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..667
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            668..737
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..851
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            852..930
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1345
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1486
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1599
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1769
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1864
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1898
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..1944
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2125
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2252
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2422
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2504
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2613
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2716
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..2785
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..4194
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3889..3894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="hexamer: AATAAA"
     regulatory      3895..3900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="hexamer: AATAAT"
     polyA_site      3911
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="major polyA site"
     polyA_site      3915
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
     regulatory      4038..4043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="hexamer: ATTAAA"
     polyA_site      4059
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
     regulatory      4165..4170
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
                     /note="hexamer: AATAAA"
     polyA_site      4194
                     /gene="Usp33"
                     /gene_synonym="9830169D19Rik; Vdu1"
ORIGIN      
        1 gacgcccgca gcggccgcgg agacaccgga aggagcggcg gctgccgagg gaagcgtcgg
       61 gcagcggtcg ggttctgcgg gccgcgctgc ggaaccgttt tgcggccctg cggatccgag
      121 cgctcaggct ccgaggagga gtgcgctggg cggcgggctg aggttttgcc tgatttgaaa
      181 agccttgaga aagagaagga aaactgtaag aaaatgacga cttttcgaaa tcattgtcca
      241 catttggatt cagttggtga aataacgaaa gaggacttga tacagaaatc tctcggcgct
      301 tgtcaggact gtaaagtcag aggaccaaac ctgtgggcgt gcctggagaa taggtgctcc
      361 tatgttggct gcggggagtc gcaagtggac cacagcacca tacactcgca ggagacaaag
      421 cattatctaa ctgtgaacct caccactctt cgagtatggt gttatgcttg cagcaaagaa
      481 gtatttttgg atagaaaatt aggaactcct ccttcattac ctcatgtaag acagcctcaa
      541 caaacacaag aaaacagtgt ccaggatttt aaaattccca gtaatccagc attgaaaacc
      601 cccatggttg ctgtgtctga agatctggat atagaagtgg aagaggaaga cgagctgaag
      661 gctagaggcc tgacaggttt gaaaaacatt ggaaatactt gttatatgaa cgcggcgctg
      721 caggctcttt ctaactgccc acctttgaca cagttctttc ttgattgtgg aggactggct
      781 agaacagata agaaaccagc aatttgtaaa agttatctca aactaatgac cgagttgtgg
      841 cacaagagca ggccaggatc tgttgtgccg gctaatctgt tccaaggaat taaaactgta
      901 aatccaactt ttcgaggtta ttctcagcag gatgcccagg aattccttcg ctgtctaatg
      961 gacctgcttc atgaggagct gaaggagcag gtcatggaaa tggaggaaga gcctcaaaca
     1021 ctaacttctg aggagacggt ggaggaagag aagagccagt cagatgtgga ttttcagtcg
     1081 tgcgagtctt gtagcagcag cgagaaagca gaaaatgaga gtggctccaa aggctttcct
     1141 gaggacagca atgagaccac catgctcatc caggacgagg atgacctgga gatggccaaa
     1201 gactggcaga aagagaaggt gtgcaataag atcaacaagg caaatgccga tgtagaactg
     1261 gacaaagaca gggacacagt gtgtgaaaca gttgacctaa acagccagga gaccgtcaaa
     1321 gtgcagatac acggcagagc ctcagaatct atcactgatg tccatctgaa tgacctagct
     1381 acgtcacaga tccttccttc aaatgaaagt gttagtccac ggttatcagc aagccctcct
     1441 aagctaggca gtctgtggcc aggactgtcg cctccacaca agaaagctca gtctacatct
     1501 gcaaagagga aaaagcagca taagaaatac agaagtgtca tctccgacat attcgatgga
     1561 acagtcatta gctcggtaca gtgtctgacg tgcgataggg tgtctataac cctcgagacc
     1621 tttcaggatc tgtccttgcc gattcctggc aaggaggacc tggctaagct gcactcctcc
     1681 agtcacccaa ctatagtcaa agcagggtca tgtggtgaag cgtacgcccc gcaggggtgg
     1741 atagctttct tcatggagta tgtgaagagg tttgttgtct catgtgtccc tagctggttt
     1801 tggggtccag tagttacctt gcaagattgt cttgctgcct tcttcgccag agatgaactt
     1861 aaaggtgaca acatgtacag ttgtgaaaaa tgcaaaaagt tgaggaatgg agtaaagttt
     1921 tgtaaagtac agaagtttcc tgagattttg tgtatccacc ttaaaagatt tcgacatgaa
     1981 ctgatgtttt ccaccaaaat tagcacccat gtttccttcc ccctggaagg cctcgatctt
     2041 cagccatttc ttgcaaagga cagcccagct cagattgtga catatgatct cctgtcagtc
     2101 atctgtcacc atgggactgc aagtagtggg cactacattg cctactgccg aaacaattta
     2161 aataacctgt ggtatgagtt tgatgaccag agcgtcactg aagtttcaga gtccactgta
     2221 cagaatgccg aggcctacgt ccttttctac aggaagagca gtgaagaggc acaaaaggag
     2281 aggcggagga tatcaaattt gttgaacatc atggaaccta gcctccttca gttctacata
     2341 tctcgacagt ggttgaataa atttaagacc tttgctgaac ctggccctat ttcaaataat
     2401 gattttctct gtatccatgg aggtattcct ccacgaaaag cgagttatat tgaagactta
     2461 gttttgatgc tgcctcagaa catttgggat aacctctata gcaggtatgg aggagggcct
     2521 gctgtcaacc atctctacat ctgccacacc tgccaaattg agttagagaa gattgaaaaa
     2581 cgaagaaaaa ccgaattgga aatttttatt cggctcaaca gagcatttca agaggaggac
     2641 tccccagcta ctttttactg tatcagcatg cagtggttta gagaatggga gagttttgta
     2701 aagggtaagg atggagatcc cccaggtcca atcgacaaca ctaaaattgc ggttactaaa
     2761 tgtggcagtg tgatgctcaa gcaaggagca gactctggtc aaatttcaga agaaacatgg
     2821 aatttcctgc agtctatata tggtgggggg cctgaagtta tcctccgacc tccagttgtt
     2881 catgttgacc ctgatgtact ccaagcagag gaaaagattg aagtagaaac tcgctctttg
     2941 tagttctgag gacacagagc tctaatgaga agttattctc acttgatgca cacacattca
     3001 aaacattccg caaagcatgt tgattctctt gattttcatc cttttttctc atttattcct
     3061 ttgtactggg cattatggaa gggtatatta aaatgttata taagttggta tatttaatgc
     3121 taagtagctt accagaatat ctttcagtgt actgttttga aagaaaaggg acagttggta
     3181 actaggttct ctgacctttg ttctacaggc agatccagca aaccctttgt agcactgtaa
     3241 atactttggc ttcgataatg ttcttattac tctaaccaat cataactggc taagcttttt
     3301 catttctcca aaaagctaat ttaattgtat attttgacat gaatttgcat agtaatgaac
     3361 ctatgttaaa tccgagagtg gtggtcagca cattgttaat ttcctaacgc tcaggaaaca
     3421 tcaggtgggt gggtgttact gcttttgcac tgcagcatat aaaactgcat attacaattt
     3481 acaaaatgtc ttttttgaat gtaacgggat gtattcattt ttagtggaat ttgttaccag
     3541 atatcagtaa attattgctg cttataatgt aaaatgttaa actttagttc tgtagacctg
     3601 aatggtattc attaatgtta gtaacaagaa aatacccctt agttttaggc cacttctatt
     3661 ttgagagcat gaagtatgga atgtgtcaaa tgagattgtt gataaagctg aaaccacttg
     3721 caaaatgatt ttttaatgaa atgcataaag tctttttgaa taatagagta ttcactgctg
     3781 tttgcttgat catgtgatgt caaaagctca actatatgca actacaaaca tgcaggtgac
     3841 tgagggacag cattcatggt gactttgcaa tgttgttaaa actcattcaa taaaaataat
     3901 tctaaaatgt ataaactgaa tgttgaattt atttgataga ctttttaaaa aaggtgtaaa
     3961 gtctgttagt attaatgcct ttcattatat cttatttgaa agcataagct aggaagttat
     4021 ttgctataat aacgtttatt aaactgtttc agaatggcag catggtggtt ggcatctgtc
     4081 tcccagtacc tggtaggctt ggctggagga acctaagttt gtgggctgta gaacaagacc
     4141 gtcccaaaac aacagctaac tataaataaa acttatcaac tgtgcattta aaaaaaaaaa
     4201 aaaaaaaaa
//
