LOCUS       NM_144937               5594 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin specific peptidase 3 (Usp3), transcript
            variant 1, mRNA.
ACCESSION   NM_144937
VERSION     NM_144937.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Karapurkar,J.K., Kim,M.S., Colaco,J.C., Suresh,B., Sarodaya,N.,
            Kim,D.H., Park,C.H., Hong,S.H., Kim,K.S. and Ramakrishna,S.
  TITLE     CRISPR/Cas9-based genome-wide screening of the deubiquitinase
            subfamily identifies USP3 as a protein stabilizer of REST blocking
            neuronal differentiation and promotes neuroblastoma tumorigenesis
  JOURNAL   J Exp Clin Cancer Res 42 (1), 121 (2023)
   PUBMED   37170124
  REMARK    GeneRIF: CRISPR/Cas9-based genome-wide screening of the
            deubiquitinase subfamily identifies USP3 as a protein stabilizer of
            REST blocking neuronal differentiation and promotes neuroblastoma
            tumorigenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   Rong,Y., Zhu,Y.Z., Yu,J.L., Wu,Y.W., Ji,S.Y., Zhou,Y., Jiang,Y.,
            Jin,J., Fan,H.Y., Shen,L. and Sha,Q.Q.
  TITLE     USP16-mediated histone H2A lysine-119 deubiquitination during
            oocyte maturation is a prerequisite for zygotic genome activation
  JOURNAL   Nucleic Acids Res 50 (10), 5599-5616 (2022)
   PUBMED   35640597
REFERENCE   4  (bases 1 to 5594)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5594)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 5594)
  AUTHORS   Lancini,C., van den Berk,P.C., Vissers,J.H., Gargiulo,G.,
            Song,J.Y., Hulsman,D., Serresi,M., Tanger,E., Blom,M., Vens,C., van
            Lohuizen,M., Jacobs,H. and Citterio,E.
  TITLE     Tight regulation of ubiquitin-mediated DNA damage response by USP3
            preserves the functional integrity of hematopoietic stem cells
  JOURNAL   J Exp Med 211 (9), 1759-1777 (2014)
   PUBMED   25113974
  REMARK    GeneRIF: USP3 deletion increased the levels of histone
            ubiquitination in adult tissues, reduced the hematopoietic stem
            cell reserves over time, and shortened animal life span.
REFERENCE   7  (bases 1 to 5594)
  AUTHORS   Belle,J.I. and Nijnik,A.
  TITLE     H2A-DUBbing the mammalian epigenome: expanding frontiers for
            histone H2A deubiquitinating enzymes in cell biology and physiology
  JOURNAL   Int J Biochem Cell Biol 50, 161-174 (2014)
   PUBMED   24647359
  REMARK    Review article
REFERENCE   8  (bases 1 to 5594)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 5594)
  AUTHORS   Sousa-Nunes,R., Rana,A.A., Kettleborough,R., Brickman,J.M.,
            Clements,M., Forrest,A., Grimmond,S., Avner,P., Smith,J.C.,
            Dunwoodie,S.L. and Beddington,R.S.
  TITLE     Characterizing embryonic gene expression patterns in the mouse
            using nonredundant sequence-based selection
  JOURNAL   Genome Res 13 (12), 2609-2620 (2003)
   PUBMED   14613977
REFERENCE   10 (bases 1 to 5594)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC132087.4, AK031141.1,
            AK158516.1, AC166755.2, AW494393.1, BM206502.2, CK624803.1,
            BF472523.1 and BE947971.1.
            
            On Oct 3, 2014 this sequence version replaced NM_144937.3.
            
            Summary: The protein encoded by this gene is a chromatin-associated
            histone 2A and 2B deubiquitinating enzyme that negatively regulates
            the DNA damage response. Mice deficient for this enzyme have
            reduced hematopoietic stem cell reserves, demonstrating a
            requirement in hematopoietic stem cell homeostasis. In addition,
            knock down of protein levels results in spontaneous tumor
            development and shortened lifespan, consistent with a function in
            preserving chromosomal integrity. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Oct 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031141.1, BC017156.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               AC132087.4         45020-45154
            136-2336            AK031141.1         1-2201
            2337-3963           AK158516.1         2017-3643
            3964-3964           AC166755.2         55967-55967
            3965-3983           AK158516.1         3645-3663
            3984-4009           AW494393.1         17-42               c
            4010-4575           BM206502.2         1-566               c
            4576-4576           AC166755.2         56579-56579
            4577-4893           CK624803.1         324-640
            4894-5204           BF472523.1         288-598
            5205-5320           BE947971.1         275-390             c
            5321-5321           AC166755.2         57324-57324
            5322-5574           BE947971.1         21-273              c
            5575-5575           AC166755.2         57578-57578
            5576-5594           BE947971.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.96 cM"
     gene            1..5594
                     /gene="Usp3"
                     /note="ubiquitin specific peptidase 3"
                     /db_xref="GeneID:235441"
                     /db_xref="MGI:MGI:2152450"
     exon            1..332
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..1804
                     /gene="Usp3"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific protease 3; ubiquitin thiolesterase 3;
                     ubiquitin carboxyl-terminal hydrolase 3; ubiquitin
                     thioesterase 3; deubiquitinating enzyme 3;
                     ubiquitin-specific-processing protease 3"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 3 isoform
                     1"
                     /protein_id="NP_659186.1"
                     /db_xref="CCDS:CCDS23305.1"
                     /db_xref="GeneID:235441"
                     /db_xref="MGI:MGI:2152450"
                     /translation="MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTC
                     SSVHCGRYVNGHAKKHYEDAQIPLLNHKRSEKQEKAQHTVCMDCSSYSTYCYRCDDFV
                     VNDTKLGLVQKVREHLQNLENSAFTADRHRKRKLLENSSLNSKLLKVNGSTTAICATG
                     LRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDSNVSL
                     VEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLEL
                     QGGFNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKF
                     DPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQ
                     KSTKKFWIQKLPKALCLHLKRFHWTAYLRNKVDTYVQFPLRGLDMKCYLLEPENSGPD
                     SCLYDLAAVVVHHGSGVGSGHYTAYAVHEGRWFHFNDSTVTVTDEETVGKAKAYILFY
                     VERQARAGAEKL"
     exon            333..393
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            394..525
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            526..609
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            610..691
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            692..774
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            775..888
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1002
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1149
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1256
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1337
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1456
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1570
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1638
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..5578
                     /gene="Usp3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1855..1860
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp3"
                     /note="hexamer: CATAAA"
     polyA_site      1880
                     /gene="Usp3"
                     /note="major polyA site"
     polyA_site      2360
                     /gene="Usp3"
                     /note="major polyA site"
     regulatory      3964..3969
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp3"
                     /note="hexamer: ATTAAA"
     polyA_site      3987
                     /gene="Usp3"
     regulatory      5557..5562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp3"
                     /note="hexamer: AATAAA"
     polyA_site      5578
                     /gene="Usp3"
ORIGIN      
        1 ctactgtccc tgggcggcgg cagaaccagg agtccggatt ttcacgttct ccggagcggg
       61 gcgccgggag acggcggatg gcaaaacccg gcgcccggaa gcccttgctt tctttgacgc
      121 aaaggctcgg gacccagaag ccgccgtctg cccagtgccg ggtcagaagc gaccccagac
      181 ggagcctctg aagtccctcc gccccagagc ctcggctgtc cccgccgccg tcctcgtggc
      241 catggagtgt ccgcacctca gctccagcgt ctgcatcgcc ccggactcag ccaagttccc
      301 caacggctcc ccgtcgtcct ggtgctgcag cgtgtgcagg tccaacaaaa gcccctgggt
      361 ctgtttgact tgttcaagtg tccactgtgg aaggtatgtg aatggccatg caaaaaaaca
      421 ttatgaagat gcacagatac ccttactcaa ccataagaga tcagaaaagc aggagaaagc
      481 tcagcataca gtgtgtatgg attgcagtag ctacagcaca tactgttatc gctgtgacga
      541 ttttgtggtc aacgacacca agctgggcct ggtacagaaa gtgagagaac acttacagaa
      601 cttggaaaac tcagccttta cagcggacag gcacagaaaa agaaaattat tggaaaactc
      661 atcactaaac agcaagttat taaaagtaaa tggaagtacg actgccattt gtgccacagg
      721 ccttcgaaat ttgggcaaca cttgtttcat gaatgctatc cttcagtcac tcagtaacat
      781 tgagcagttt tgctgttatt tcaaagaact gcctgctgtg gagttaagga atgggaaaac
      841 agcaggaagg cgaacatacc acaccaggag ccaaggggac agcaatgtgt ctttagtaga
      901 agagtttaga aagacactgt gtgctttatg gcaaggcagc cagactgcat ttagtcctga
      961 gtccttgttt tatgttgtct ggaagatcat gcctaacttt aggggttatc agcagcagga
     1021 tgcccatgaa ttcatgcgct accttttgga tcatctacac ttagaacttc agggtggctt
     1081 caatggtgtt tcccgctcag caattctaca ggagaattct acactgtctg caagtaacaa
     1141 atgctgcata aatggagcat caactgttgt cacagcaata tttggaggaa ttctccagaa
     1201 cgaggtcaac tgcctcatat gtgggacaga gtcgaggaag tttgatccat tcctagatct
     1261 ctccttagat attccaagtc agttcagaag caagcgctct aagaatcagg aaaatgggcc
     1321 agtttgctcc ttaagagatt gccttcgcag cttcactgac ctagaggaac ttgatgagac
     1381 agagttgtat atgtgccata agtgcaaaaa gaaacaaaag tccacaaaga agttttggat
     1441 tcaaaaactt cccaaggcac tgtgcttgca cctgaaaagg tttcactgga cagcatattt
     1501 aagaaacaaa gtcgatacat acgttcaatt cccactgaga ggcctggaca tgaagtgcta
     1561 cctactagag ccggagaaca gtggtccaga cagttgcctg tatgacctcg ctgctgtggt
     1621 tgtacaccat ggctctgggg ttggttctgg acattacaca gcatatgcag ttcacgaagg
     1681 ccgctggttc catttcaatg acagcactgt gacagtgact gatgaggaga ccgttgggaa
     1741 ggcaaaggcc tacattctgt tttatgtgga gcgtcaggcc agagctggag cagaaaaact
     1801 gtaatacctc ctgaccatga gtcatcaact acaccggaca acatttccat tttccataaa
     1861 tacttgatcc aagagtcaaa tttcagtatg cacttttcaa tttccagttt ggggtttagt
     1921 ttggtcagtg gtagtgactt acccaacatg agcaccaact aatttttgtt gttgttctac
     1981 cagaaaacct cagcaggcat tttgattggc tgctttagtt gtaataattc aatttttata
     2041 tgtagtttcc agaacttagt tctgtttgac tttttatatt aatgttttca gttctcaccg
     2101 aggcacattt gcatttgctc tatactcaca tgctgagagc caccctgact gctgtcttca
     2161 tagcagccag gtcagcgact ccatcgtgag gatcgtgtgt gcaggtctgt ggcccaccgg
     2221 tttcacagtg actgctcagg aattccttcc gactgcagac caggccgtca ccactgctgt
     2281 gtctcagggt tgctgtgcct cagcattaaa taactctgcc agtcccgttg cacctgtgct
     2341 agagttccag aactgctcca tttcttgtct ctggtcttct tttaatattt tcttgggggc
     2401 agacaggcta ggaacactga caagtgcaaa tacgtgttta aaagccctgc gattcagctg
     2461 catggtagag gtatgtcctg tgggtggatc tgtctgacca tcagaggtga ggtcttaccc
     2521 cccagacctg acttccaggc agaagtggag tcatagaagc tctgggtttt aattcactcc
     2581 ctgcctgaag cagaaccagg tgaaagtata tgtctctgaa caagaatagt ttttattaaa
     2641 ttcagtagct taaaaaatac tgttcacaga aataggttag aagcactatt tggaccattt
     2701 aaagaaggca gctagtggca aatgctggag gccagtgatt cggtttgcag gtattgaaac
     2761 ctttaagtca gagtgtacaa ctccaacaca gcctgttcca catccacctc ctcgagggac
     2821 aaaatgcact tcatgcagac tttttactaa tattttttct attacaagta agtttggtag
     2881 ctttccttgt ccagtgaagt tgatggagac gtggctcaat tccacattaa tattagctac
     2941 agaaatcttc attccctcag aacagaaatt acattctgga tcagtacctt aatacactaa
     3001 cttgaatccc tcagtacaat gaccgtatga ctagtgtgtc cttggtcaca ctagttatgt
     3061 cagggctggg tacaggacag tgcagggcaa gtacctttcc atagggttct cttgcacaac
     3121 acaggtctaa attttatgca gaacaacaaa gctgtgacat gtgtaagcac tacaacttag
     3181 atttagtgca ggaagaggaa gtgtgctgag ttccatgagg tagaggcatg tgcagctcta
     3241 gttctgctgg tttgagccac agatcgaggg tcgaatgatt tgctctaata tatcagaact
     3301 gtgataacat cacagctaag cctaaatgag tctcatctaa cgttgcacac agctcccact
     3361 aatccaggct cctctggcat ttatatcttt gcctctgact gggcaactgt aatgacttac
     3421 acagtacgca cacagagctg gcactgtcaa gccaggtgga gcagcagcag cagactgggc
     3481 cgctctgtgg ggagagtggt cattaggcaa cactttggca tacaagggaa tgaatcatct
     3541 ctggaatttt tgtagcagct ttattgcttg gttttactta agcagcaatg gctgagtgac
     3601 accatgaaga ctctttgaga aagtgactgc acccatatgc agtgcccatc tgaacaccca
     3661 tgatgaagta catgtgccta tttgttttaa atctagcctt actgcaccgg ttacaggctt
     3721 ctctctgtac tgtctagggc tttcccacgt gtgttctaac ctgcatgcaa cttttgctca
     3781 gagcgtatgc tgaggcaggg cctcatgcat atcctgtcct gtcagcccat ggcaacagga
     3841 aactccaagt cctcccacac aggtggggat agaaacagtt tgtggcaaca caagtctctc
     3901 catggtggag aactctaagc ttatccactg tattctactc agtgtgtagt ggcagatgat
     3961 gtaattaaac cagtcatatg cgctggccct ggttgagtgt cgtgtgtcac acctgtggta
     4021 cctgctcaga tttatttcac ttctttggtt tactttatat gtttaaccca ttgtaacctc
     4081 taccgtcccc taaactctct catggtcctc caactgctga agtcacctct tgcccaggct
     4141 ttggaaaggc tcaggtagga actgtttcca gagcatgggt cttctgacag ccccgagaag
     4201 atgccacagc acaggaaaga ggaggcacat gtgtgtgtga ctgtcaccac tgggcagtag
     4261 ccctcaaggg agtggtggcc atgccaagat cattggccat taagacttag tagtctgtat
     4321 gatgccaaga tgtcattgtg cacgttacca ttcattctgg ctgcagacga atcacatcct
     4381 agtcccgcct caccagctgg gctcttctgt ggattgtttc caggcagagc aagccgggca
     4441 gtggtggcgc tcgcctttag ttccagcact caggaggcag aggcaggcgg atttctgagt
     4501 tcgaggccag cctggtctac aaagtgagtt ccaggacagc cagggatata cagagaaacc
     4561 ctgtctcaaa aagccaaaaa aaaaaaaaaa aaaaaaaaag aggaggagga gaggaaggaa
     4621 gaagaaatgg cagagcaaca gccatcaggg gccagtcttg tagcctacat gggcaggagg
     4681 aaaggggagt agtcattaag gggttctgtg gagttctttc aggggaagct gtagtagtct
     4741 taggtttcca ccaagttgta tgcacttaga gcagcttcat acttccagat ctgcagcaca
     4801 gcttcctaaa aagcagggca gctgggggct gtgtgcaagc attgaaaccc atcagacttg
     4861 cagttcactt ccagttattt acacttcaat taagtgttca aaaatagaaa ctgcccaccc
     4921 tcacatattg gggaatgcta tctaggacag ataatgaaac tctgcaaatc acccacgaaa
     4981 aagaacacat gcaccagtct gtctgtctca cacacacgtg ctagctttta aaataacatt
     5041 tcagatgaat cttatgacag tttagggtaa aaatggagcc atttgggcct tgccattggg
     5101 gtttaaagat agctcatctt tgcaaggtag ccattagtgt cctgagctaa agtaccaagc
     5161 atgatcctca gattccactc acagtttggg gcatggtaaa aatgacctct tcctagcaag
     5221 ggtgagcaga ggagccgcac cgacacactc tgatacctaa cagctaatac tcactgtcct
     5281 gctaggagaa aggctgcaag cccaggaagg gctgttgtcg ctaattacct ccaatgaaaa
     5341 caccttagta aaagccaaga gccctgtgct caacacttat cagctcagag gaaaagaaat
     5401 cccttttaag gaaaacttgt aacttcaatg ggctgctctt ctgccaggac tttcaggccc
     5461 tctgaacata agccgaacca cagatggctt cgagtaagtc tggatcctga cacccgccct
     5521 ccccaccccg agcttcagca ccagttgttg gccaaaaata aacaccattc ctcaaccaaa
     5581 aaaaaaaaaa aaaa
//
