LOCUS       NM_028846               4157 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ubiquitin specific peptidase 20 (Usp20), mRNA.
ACCESSION   NM_028846
VERSION     NM_028846.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Zhang,F., Liu,C., Chen,Z. and Zhao,C.
  TITLE     A novel PDIA3/FTO/USP20 positive feedback regulatory loop induces
            osteogenic differentiation of preosteoblast in osteoporosis
  JOURNAL   Cell Biol Int 48 (4), 541-550 (2024)
   PUBMED   38321831
  REMARK    GeneRIF: A novel PDIA3/FTO/USP20 positive feedback regulatory loop
            induces osteogenic differentiation of preosteoblast in
            osteoporosis.
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4157)
  AUTHORS   Zhang,L., Wu,J.H., Jean-Charles,P.Y., Murali,P., Zhang,W.,
            Jazic,A., Kaur,S., Nepliouev,I., Stiber,J.A., Snow,K.,
            Freedman,N.J. and Shenoy,S.K.
  TITLE     Phosphorylation of USP20 on Ser334 by IRAK1 promotes
            IL-1beta-evoked signaling in vascular smooth muscle cells and
            vascular inflammation
  JOURNAL   J Biol Chem 299 (7), 104911 (2023)
   PUBMED   37311534
  REMARK    GeneRIF: Phosphorylation of USP20 on Ser334 by IRAK1 promotes
            IL-1beta-evoked signaling in vascular smooth muscle cells and
            vascular inflammation.
REFERENCE   4  (bases 1 to 4157)
  AUTHORS   Zhang,W., Shi,B., Li,S., Liu,Z., Li,S., Dong,S., Cheng,Y., Zhu,J.,
            Zhang,G. and Zhong,M.
  TITLE     Sleeve gastrectomy improves lipid dysmetabolism by downregulating
            the USP20-HSPA2 axis in diet-induced obese mice
  JOURNAL   Front Endocrinol (Lausanne) 13, 1041027 (2022)
   PUBMED   36636478
  REMARK    GeneRIF: Sleeve gastrectomy improves lipid dysmetabolism by
            downregulating the USP20-HSPA2 axis in diet-induced obese mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4157)
  AUTHORS   Xiao,X. and Tontonoz,P.
  TITLE     USP20 links feeding-induced cholesterol synthesis and energy
            expenditure
  JOURNAL   Sci China Life Sci 64 (2), 337-338 (2021)
   PUBMED   33420925
  REMARK    GeneRIF: USP20 links feeding-induced cholesterol synthesis and
            energy expenditure.
REFERENCE   6  (bases 1 to 4157)
  AUTHORS   Jean-Charles,P.Y., Wu,J.H., Zhang,L., Kaur,S., Nepliouev,I.,
            Stiber,J.A., Brian,L., Qi,R., Wertman,V., Shenoy,S.K. and
            Freedman,N.J.
  TITLE     USP20 (Ubiquitin-Specific Protease 20) Inhibits TNF (Tumor Necrosis
            Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates
            Atherosclerosis
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (10), 2295-2305 (2018)
   PUBMED   30354204
  REMARK    GeneRIF: USP20 attenuates TNF- and IL-1beta-evoked atherogenic
            signaling in smooth muscle cells, by deubiquitinating RIPK1, among
            other signaling intermediates.
REFERENCE   7  (bases 1 to 4157)
  AUTHORS   Jean-Charles,P.Y., Zhang,L., Wu,J.H., Han,S.O., Brian,L.,
            Freedman,N.J. and Shenoy,S.K.
  TITLE     Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions
            of beta-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor
            kappaB (NFkappaB) Activation
  JOURNAL   J Biol Chem 291 (14), 7450-7464 (2016)
   PUBMED   26839314
  REMARK    GeneRIF: that pro- and anti-inflammatory activities of
            beta-arrestin2 are determined by beta-arrestin2 ubiquitination and
            that changes in USP20 expression and/or activity can therefore
            regulate inflammatory responses
REFERENCE   8  (bases 1 to 4157)
  AUTHORS   Lumayag,S., Haldin,C.E., Corbett,N.J., Wahlin,K.J., Cowan,C.,
            Turturro,S., Larsen,P.E., Kovacs,B., Witmer,P.D., Valle,D.,
            Zack,D.J., Nicholson,D.A. and Xu,S.
  TITLE     Inactivation of the microRNA-183/96/182 cluster results in
            syndromic retinal degeneration
  JOURNAL   Proc Natl Acad Sci U S A 110 (6), E507-E516 (2013)
   PUBMED   23341629
REFERENCE   9  (bases 1 to 4157)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   10 (bases 1 to 4157)
  AUTHORS   Li,Z., Wang,D., Na,X., Schoen,S.R., Messing,E.M. and Wu,G.
  TITLE     Identification of a deubiquitinating enzyme subfamily as substrates
            of the von Hippel-Lindau tumor suppressor
  JOURNAL   Biochem Biophys Res Commun 294 (3), 700-709 (2002)
   PUBMED   12056827
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY251374.1, AK173083.1, AK047242.1 and AK054279.1.
            
            On Apr 22, 2010 this sequence version replaced NM_028846.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK054279.1, AF449715.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                BY251374.1         2-99
            99-3176             AK173083.1         1-3078
            3177-4145           AK047242.1         3096-4064
            4146-4157           AK054279.1         4089-4100
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.77 cM"
     gene            1..4157
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /note="ubiquitin specific peptidase 20"
                     /db_xref="GeneID:74270"
                     /db_xref="MGI:MGI:1921520"
     exon            1..120
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            121..220
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            221..316
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..2986
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin specific protease 20; ubiquitin
                     thioesterase 20; deubiquitinating enzyme 20;
                     VHL-interacting deubiquitinating enzyme 2;
                     ubiquitin-specific-processing protease 20; ubiquitin
                     thiolesterase 20"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 20"
                     /protein_id="NP_083122.1"
                     /db_xref="CCDS:CCDS15893.1"
                     /db_xref="GeneID:74270"
                     /db_xref="MGI:MGI:1921520"
                     /translation="MGDARDLCPHLDCIGEVTKEDLLLKSKGTCQSCGVAGPNLWACL
                     QVTCPYVGCGESFADHSSIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAVHLAS
                     SSARLSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAAL
                     QALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIK
                     LVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGD
                     RSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFS
                     WGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRSTSPCQTPEPDNEAHIRSSSR
                     PCSPVHHHHEGHSKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDV
                     FNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDS
                     YSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCK
                     KLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTS
                     QVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVL
                     FYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSH
                     GGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRV
                     EIDTFIKLNKAFQAEESPAVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGS
                     GHIQLKQGADCGQISEETWTYLSSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETR
                     AL"
     exon            317..370
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            371..433
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            434..565
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            566..662
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            663..732
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            733..846
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..925
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1373
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1517
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1633
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1806
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1901
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..1935
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..1981
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2162
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2289
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2459
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2541
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2650
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2753
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2825
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..4157
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3200..3205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /note="hexamer: AATATA"
     polyA_site      3226
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /note="major polyA site"
     regulatory      4119..4124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /note="hexamer: AATAAA"
     polyA_site      4147
                     /gene="Usp20"
                     /gene_synonym="1700055M05Rik; Vdu2"
                     /note="major polyA site"
ORIGIN      
        1 ggctctcccg attggacgtc tgacggtgag gaggcggggc gtggcaggca gagccacaag
       61 actctaggca gcgccgcggt gggctagctt gactgacagg cggcgcggtt gcgacggcag
      121 gtacttggct caagacttta ctgcagagcc cctcggcagc tggtcactgt cccaagagaa
      181 tgcccacatc ggcctcctcc tgtgtgtgca gctgagtcag gggaggctgg ccagaatggg
      241 ggacgccaga gacctctgcc ctcacctgga ctgcataggg gaggtgacca aagaggattt
      301 gctgcttaaa tctaagggaa cctgccagtc atgtggggtt gctggaccta acctatgggc
      361 ttgcctccag gtcacctgcc cttacgttgg ctgtggagag tcttttgctg accatagcag
      421 cattcatgcc caggtgaaaa agcacaacct gactgtgaac ctgaccacgt tccgggtctg
      481 gtgctacgcc tgtgagcgtg aagtctttct ggagcagcgt ctggcagtcc acctggccag
      541 ctcctcagcc aggctctcag agcaggactc cccaccaccc tcccacccgc tgaaagctgt
      601 ccctattgct gtggctgatg aaggagagtc tgagtctgag gatgacgacc tgaagcctcg
      661 aggcctcaca ggcatgaaga acctgggaaa ctcctgttac atgaatgctg ccctgcaggc
      721 cttgtccaat tgccccccgc tcacccagtt cttcctggag tgtggtggtc tggtgcgcac
      781 agacaagaag ccagccttgt gcaaaagtta ccagaagttg atctctgagg tgtggcacaa
      841 gaagcggcca agctatgtgg tccccaccag cctgtcccat gggatcaagt tggtcaaccc
      901 gatgttccga ggctatgccc agcaggacac ccaggagttc ctacgctgcc taatggacca
      961 gcttcatgag gagctcaagg agcccatggt cgctgctgtg gcagcactca ccgatgctcg
     1021 ggactccgat tccagtgaca cagatgagag gcgtgatggc gaccggagcc catcagagga
     1081 cgagtttctg tcctgtgact cgagcagtga caggggtgag ggtgatgggc aggggcgtgg
     1141 tggaggcagc tcaaaggccg agatggagct gctgatctca gatgaggcgg gccgagccat
     1201 ctctgagaag gagcggatga aggaccgcaa gttctcctgg ggccagcagc gcaccaactc
     1261 agagcaagtg gatgaggatg cagatgtgga cacggccatg gcgtcccttg atgagcaatc
     1321 cagagaggcc cagccaccat caccaaggtc caccagcccc tgccagaccc cagaaccaga
     1381 caacgaagcc cacatacgca gctcctctcg tccctgcagc cctgttcacc accaccacga
     1441 gggccattcc aagctgtcca gcagccctcc tcgtgcaagc cctgtgagga tggggccgtc
     1501 gtatgtgctc aagaaagccc aggtgccaag cactggtggt cggaggcgga aggagcagag
     1561 ctaccgcagt gtcatctcag acgtcttcaa tggctctgtc ctcagcctgg tgcagtgtct
     1621 tacctgtgac cgggtgtcta ccacagtgga gacattccag gacctgtcat tgcccatccc
     1681 tggcaaggaa gatctagcca aactccactc tgccatctac cagaatgtac cagccaagcc
     1741 gggtgcctgt ggggacagct attcctccca gggctggctg gccttcatcg tggagtacat
     1801 cagacggttt gtggtatcct gtacccccag ctggttttgg ggacctgttg tcacactaga
     1861 agactgcctt gctgctttct ttgctgctga tgagctgaag ggtgacaaca tgtacagctg
     1921 tgagcggtgt aagaagcttc ggaatggcgt gaagtactgt aaagtcctat gtctgcctga
     1981 gattctgtgc gtccacctga agcgcttccg gcatgaggtg atgtactcct tcaaggtcag
     2041 cagccatgtc tctttccccc tcgagggact ggacctacgc ccttttctgg ccaaggagtg
     2101 cacgtcccag gtcaccacct atgacctcct ctctgtcatc tgtcaccatg gcacagcagg
     2161 cagtgggcac tacattgcct actgtcagaa cgtgatcaat gggcagtggt acgaattcga
     2221 tgaccagtac gtcaccgagg tccatgagac ggtagtacag aacgtggagg cctacgtgct
     2281 gttctacagg aagagcagtg aggaggccat gcgtgagcgg cagcaggtag tgtccctggc
     2341 tgccatgcgg gaacccagcc tgctccgctt ctacgtgtcc cgagaatggc tcaacaaatt
     2401 caacaccttt gccgagccag ggcccatcac caaccacacc ttcctgtgct cccatggagg
     2461 tatcccaccc aacaaatacc actacatcga tgacctggtg gtcatcctgc cgcagagcgt
     2521 ctgggagcac ctgtacagca ggtttggggg cggccctgct gtgaaccatc tgtatgtgtg
     2581 ctccatctgc caggtggaga ttgaggccct ggccaagcgc aggagggtgg agattgacac
     2641 tttcatcaag ctgaacaagg cattccaagc tgaggagtct cccgcggtca tctactgtat
     2701 cagcatgcac tggttccgag agtgggaggc cttcgtcaag gggaaggaca gtgagcctcc
     2761 cgggcccatc gacaacagca ggattgccca ggtcaaaggc agtggccata tccagctaaa
     2821 gcagggggct gactgtggcc agatttcgga ggagacctgg acctatctga gcagtctgta
     2881 tgggggcggc cctgagattg ccatccggca gagtgtggcc cagctcccag acccagaaag
     2941 cttgcacgga gagcagaaga ttgaggctga gacccgggct ctgtgacttg ggccatgctg
     3001 agttgctgtg gctggcccta ctccctctcc agacagcgct gcatccctct gaaggctgtg
     3061 tcctgctgag tgcccactgt ccctgaaggc tggtgttcga cattccccag agggtgtgtc
     3121 tgccctgcag ctgagctgct gtgtgcctgg tacagaggct gagagcggct ggtccgccct
     3181 gagagatgct gtgctgggaa atatattcgc tcggaggact ctgtcatcgc tgtgtcccca
     3241 aatttgccct cttgctaact tgggcccagc ttttgtccca ggagccccat gctgcccctt
     3301 aggtaatgct gacccctggg atggggggct aggagcagct gcaacctttt gggtattatc
     3361 tgctgtatac ttggcccaac cccttgggga ctcagagaag tagatgtcta cacacagggg
     3421 cagaatggat gcagggctct ttgtgtaggc cagcagcccc tatatcatga tggagacccc
     3481 tgctgcccgt gtttgttctg ttgactattg tagtgggaca gagaaagggc aagctttgct
     3541 ttagagggta gaggacctgc caccttgtcc tgctgagacc actggcagac caactgtcat
     3601 ttgctctgtg aagaggctcc taagaccctg ggggagggaa aacagatacc tcatgcactg
     3661 gcccactgtc tgacgctcct tcatcctctg tgcggtctgg agtgagcctg gtcccctagc
     3721 caccccctag ccttggtgtc ttgagccaat tctgagatgt ggtctgtgtc ctggacctgc
     3781 tgagcctaga gagattgtcc tatgtatgtc tgagatcctg gagaaaccta accgaacttc
     3841 cagggcaggg tgttcaaagc taagtctgga gtggtgaggg ggggatcctg ggaccaagag
     3901 ggctccagtg gccacctgtc tcttcagaag tgggtaccag cagctcatcc ccaaaggcct
     3961 gggtgttcag tgtcccactg tggtgggtgg caagtgggtc catggccggc tgctgctggg
     4021 acgtgccctg ttggctgcca tcttttgaag tctctgttgt agagccctta gctcattgca
     4081 ctactgtgac gcagtctggt ttgtgttcag aatgtcacaa taaatacttg ttcctctcct
     4141 gcccctagct tgtactg
//
