LOCUS       NM_001417623            3899 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus USO1 vesicle docking factor (Uso1), transcript variant
            1, mRNA.
ACCESSION   NM_001417623 XM_006535140
VERSION     NM_001417623.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Giacomello,E., Ronchi,P. and Pepperkok,R.
  TITLE     GM130 and p115 play a key role in the organisation of the early
            secretory pathway during skeletal muscle differentiation
  JOURNAL   J Cell Sci 132 (2) (2019)
   PUBMED   30630895
  REMARK    GeneRIF: knockdown of GM130 (also known as GOLGA2) or p115 (also
            known as USO1), two regulators of the early secretory pathway,
            impairs GC and ERES reorganisation. This in turn results in
            inhibition of myotube fusion and M-cadherin (also known as CDH15)
            transport to the sarcolemma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3899)
  AUTHORS   Cinque,L., Forrester,A., Bartolomeo,R., Svelto,M., Venditti,R.,
            Montefusco,S., Polishchuk,E., Nusco,E., Rossi,A., Medina,D.L.,
            Polishchuk,R., De Matteis,M.A. and Settembre,C.
  TITLE     FGF signalling regulates bone growth through autophagy
  JOURNAL   Nature 528 (7581), 272-275 (2015)
   PUBMED   26595272
REFERENCE   4  (bases 1 to 3899)
  AUTHORS   Kim,S., Hill,A., Warman,M.L. and Smits,P.
  TITLE     Golgi disruption and early embryonic lethality in mice lacking USO1
  JOURNAL   PLoS One 7 (11), e50530 (2012)
   PUBMED   23185636
  REMARK    GeneRIF: USO1 deficiency results in Golgi disruption and early
            embryonic lethality in mice.
REFERENCE   5  (bases 1 to 3899)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   6  (bases 1 to 3899)
  AUTHORS   Hosaka,T., Brooks,C.C., Presman,E., Kim,S.K., Zhang,Z., Breen,M.,
            Gross,D.N., Sztul,E. and Pilch,P.F.
  TITLE     p115 Interacts with the GLUT4 vesicle protein, IRAP, and plays a
            critical role in insulin-stimulated GLUT4 translocation
  JOURNAL   Mol Biol Cell 16 (6), 2882-2890 (2005)
   PUBMED   15800058
REFERENCE   7  (bases 1 to 3899)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122438.2 and AC125542.4.
            
            On Mar 7, 2023 this sequence version replaced XM_006535140.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.6182042.1,
                                           SRR13862062.151337.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AC122438.2         3079-3279           c
            202-288             AC125542.4         70770-70856
            289-353             AC125542.4         71181-71245
            354-430             AC125542.4         76733-76809
            431-531             AC125542.4         84860-84960
            532-634             AC125542.4         85355-85457
            635-690             AC125542.4         85607-85662
            691-811             AC125542.4         89002-89122
            812-990             AC125542.4         92255-92433
            991-1131            AC125542.4         95764-95904
            1132-1220           AC125542.4         98871-98959
            1221-1375           AC125542.4         99669-99823
            1376-1587           AC125542.4         100227-100438
            1588-1608           AC125542.4         101811-101831
            1609-1719           AC125542.4         105564-105674
            1720-1838           AC125542.4         106027-106145
            1839-2020           AC125542.4         106272-106453
            2021-2142           AC125542.4         107552-107673
            2143-2281           AC125542.4         111042-111180
            2282-2445           AC125542.4         112341-112504
            2446-2532           AC125542.4         113677-113763
            2533-2631           AC125542.4         116665-116763
            2632-2739           AC125542.4         118146-118253
            2740-2955           AC125542.4         119464-119679
            2956-3899           AC125542.4         120186-121129
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 46.05 cM"
     gene            1..3899
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /note="USO1 vesicle docking factor"
                     /db_xref="GeneID:56041"
                     /db_xref="MGI:MGI:1929095"
     exon            1..201
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..3036
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transcytosis associated protein p115; general vesicular
                     transport factor p115; protein USO1 homolog;
                     vesicle-docking protein; transcytosis-associated protein;
                     USO1 homolog, vesicle docking protein; vesicle docking
                     protein, 115 kDa"
                     /codon_start=1
                     /product="general vesicular transport factor p115 isoform
                     1"
                     /protein_id="NP_001404552.1"
                     /db_xref="GeneID:56041"
                     /db_xref="MGI:MGI:1929095"
                     /translation="MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVR
                     ALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIAYALDTLYNIISNDEEEELEENS
                     TRQSEDLGSQFTEIFIKQPENVTLLLSLLEEFDFHVRWPGVRLLTSLLKQLGPPVQQI
                     ILVSPMGVSRLMDLLADSREIIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIIT
                     EEGNSDGGIVVEDCLILLQNLLKTNNSNQNFFKEGSYIQRMKPWFEVGEENSGWSAQK
                     VTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGIPADILTETI
                     NTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCF
                     LYKNEKGQGEIVATLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQG
                     NATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGDKIDRRGSKIQTRVGLLMLLCTW
                     LSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLENY
                     TKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLV
                     KELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELKQQVST
                     LKCQNEQLQTAVTQQASQIQQHKDQYNLLKVQLGKDNHHQGSHGDGAQVNGIQPEEIS
                     RLREEIEELKSQQALLQGQLAEKDSLIENLKSSQASGMSEQASATCPPRDPEQVAELK
                     QELTALKSQLCSQSLEITRLQTENCELLQRAETLAKSVPVEGESEHVSAAKTTDVEGR
                     LSALLQETKELKNEIKALSEERTAIQKQLDSSNSTIAILQTEKDKLDLEVTDSKKEQD
                     DLLVLLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDDEIDDGDKDQDI"
     exon            202..288
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            289..353
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            354..430
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            431..531
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            532..634
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            635..690
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            691..811
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            812..990
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1131
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1220
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1375
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1587
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1608
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1719
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1838
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..2020
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2142
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2281
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2445
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2532
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2631
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2739
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2955
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..3899
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3878..3883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /note="hexamer: AATAGA"
     polyA_site      3899
                     /gene="Uso1"
                     /gene_synonym="115kDa; TAP; Vdp"
                     /note="major polyA site"
ORIGIN      
        1 gctgagtagg aggcggcggt ggcggaggcc ggggagtgcg gagagccggc tcgggggcta
       61 ggccctacgg ggggcggggg gcggtcgtgt tttctttggc agaggggctg gtgaagccgg
      121 cggctgagcg acaagatgaa tttcctccgt ggggtgatgg ggggtcagag tgccggaccc
      181 cagcacactg aagccgagac gattcagaag ctctgtgacc gcgtagcttc ctctacgtta
      241 ctggatgacc gaagaaatgc tgtacgtgcc cttaaatcac tctctaagaa ataccgctta
      301 gaagtgggaa tccaagctat ggaacatctt atccatgttt tacaaacaga tcgttcggat
      361 tctgaaataa tagcatatgc tttggacaca ctttataata taatatctaa tgacgaagag
      421 gaagaactag aagaaaattc tacaagacag agtgaggatt tgggaagcca gtttacagaa
      481 attttcatta agcagccaga aaatgtcact ctcctgttat ctttgttgga ggagtttgat
      541 ttccacgtcc gttggcctgg cgtgagactc ctgacttctc ttttaaagca gctagggcct
      601 cccgtgcagc agatcatcct agtcagcccc atgggtgttt caagattaat ggacttacta
      661 gcagattcca gagaaattat acgcaacgat ggcgtcttac tactgcaggc attaacaagg
      721 agcaatggag caatccagaa aattgttgct tttgaaaatg cttttgagcg actgctggac
      781 attattacag aggaggggaa cagtgacgga ggtatagtag tggaagattg tttgatttta
      841 cttcaaaact tgttaaaaac caacaattcc aatcaaaact tttttaaaga aggctcttat
      901 attcaacgca tgaaaccttg gtttgaagtt ggagaggaaa actctggttg gtcagcacag
      961 aaagtgacca atctacattt aatgctccag cttgtgcggg tactggtatc tcccaccaac
     1021 cctcccggtg ctaccagtag ctgccagaag gccatgttcc agtgtgggct actccagcag
     1081 ctttgcacta tcctgatggc tactgggatt cctgctgata tcctgacaga gaccataaat
     1141 actgtatcag aggttatccg aggctgccaa gtaaaccaag actactttgc ttctgtgaat
     1201 gcaccttcaa atcccccacg accggcaatt gttgtgctgc tcatgtccat ggtcaacgag
     1261 cggcagccgt ttgtgctgcg atgtgccgtg ctctactgtt tccagtgctt cctgtataaa
     1321 aacgagaaag gacaaggaga gatcgtggcc actcttctgc cttccactat tgatgcaaca
     1381 ggtaactccg tctcagccgg tcagctgctc tgcggaggtt tgttttctac agactctctc
     1441 tctaactggt gtgctgctgt ggccctcgcc catgcgctgc agggaaatgc cacccagaaa
     1501 gagcaactgc tcagggttca gctggccacc agcatcggca accctccggt gtcgctcctg
     1561 cagcaatgca ccaacattct ttcccagggt gataagatcg acagacgggg aagcaaaata
     1621 cagacaagag tcgggttgtt aatgttgctc tgtacctggc taagcaactg tcctattgct
     1681 gtaacacatt ttcttcacaa ctcagccaat gttccattcc ttacaggaca gattgcagaa
     1741 aatctcggag aagaagagca gttggtccaa ggcttatgtg cccttctttt gggcatttca
     1801 atttatttca acgataactc actagaaaac tacacaaaag agaaactaaa gcaactaata
     1861 gagaagagga ttggcaaaga gaatttcata gagaaactag gatttattag caaacatgag
     1921 ttatattcca gagcgtctca gaaaccccag ccaaactttc cgagtccaga gtatatgata
     1981 tttgatcatg agtttacaaa gctggtgaaa gaactcgaag gtgttattac caaggctatt
     2041 tataagtcca gtgaggaaga taagaaagag gaagaggtaa agaaaacctt agagcagcat
     2101 gataacatcg tgactcacta taagaatatg atccgtgagc aagacctaca actggaggaa
     2161 ctgaaacagc aagtctccac cctgaagtgt cagaacgagc agctacagac agcagtcacc
     2221 cagcaggcat ctcagattca gcagcacaag gaccagtaca atctcctcaa agttcagctc
     2281 ggaaaagaca atcaccatca aggttcccac ggtgacgggg ctcaggtgaa tggcattcag
     2341 ccggaagaaa tcagtcggct gagggaagag atagaagagt taaaaagtca gcaggcgctc
     2401 ttacaaggcc agctagctga aaaggactca ttgattgaaa atttgaaatc ttcgcaagcg
     2461 tctggcatga gtgaacaggc ttcagcaaca tgtcctccca gagatcccga gcaagttgca
     2521 gagttaaagc aggaactgac agcattaaag tcccagttat gttcacagtc tctggagatc
     2581 acgagactcc agacggagaa ctgcgagctg ctgcagagag cagagacctt ggcaaaatca
     2641 gttcctgtag aaggagagag tgagcatgtg agtgctgcta agaccacgga tgtagaagga
     2701 aggctgtctg ctttgctgca ggagaccaaa gagttaaaga atgagattaa agcgctgtct
     2761 gaggagagga ccgccattca aaagcagctg gactcatcca atagcaccat cgccatcctg
     2821 caaaccgaga aagacaagct ggacttggaa gttacggatt ctaagaaaga acaagatgac
     2881 cttttggtgc tgttagcaga tcaagatcag aaaatcctgt cactgaagag taaactcaag
     2941 gatctcggtc atccagttga agaagaagat gaatctggag accaagaaga cgatgatgat
     3001 gaaattgatg atggtgacaa ggaccaggac atctagcttt aaaccctagg aatgatagag
     3061 attacgtcat tatgtggaag aagtcttaac agtgctttgg tttgcctcca cacaaaaaat
     3121 aaagacagaa ccaggtggaa acattgacta gtaaaactat tgatatatgt tttttaatat
     3181 tgccacccat gtggaaaact gacttcatgt ggaactttta tttatgttca tctgaactct
     3241 cccaaaataa aatttgcaca gggcaccaag ctccaaaagt accccctttg aaggcatgtg
     3301 gtggcaatgc agtttgccat gttggttcac tgatggcttt cagacactct gtgacttcag
     3361 tactcacaca tttgattaat gtgtattcaa tgggagtcat catttctaag tcagcgtttg
     3421 agttctcggc tgattaatcc ttgtaaagaa aggactgtac ttagggtagc ctggggctcc
     3481 tgtatgtcat cccctcaaac ccccactgtg cctcccgaga tcaggatggg acagttccga
     3541 gtgagtgctt gccgtccagc ggggccacat tctgcctact gagaggaaag attgtcccag
     3601 agtgtggagg gcgttctgag gaactggaac agtgtactaa tgtggctggt tttctctctg
     3661 tttaagctaa agatggaaaa cagattatag tcagcagttc atatagctgg gatttagaat
     3721 tgtttgatta tcataaaaca tgttcttagt tttaaaaaat cccacactat gatttatgta
     3781 atttattggc atttttgctg aataggtgta agtcagataa tagattttta aaaagcaaat
     3841 gaagcaatgt gtcaaagtct aatgttttca taataaaaat agatattatg ttcacttaa
//
