LOCUS       NM_001081189            7052 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus uracil phosphoribosyltransferase (Uprt), mRNA.
ACCESSION   NM_001081189 XM_284721
VERSION     NM_001081189.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Krassikova,L.S., Karshieva,S.S., Cheglakov,I.B. and Belyavsky,A.V.
  TITLE     Combined treatment, based on lysomustine administration with
            mesenchymal stem cells expressing cytosine deaminase therapy, leads
            to pronounced murine Lewis lung carcinoma growth inhibition
  JOURNAL   J Gene Med 18 (9), 220-233 (2016)
   PUBMED   27461566
  REMARK    GeneRIF: Data indicate that a combination cytosine deaminase
            (CDA)/uracil phosphoribosyltransferase protein (UPRT)-expressing
            adipose tissue-derived mesenchymal stem cells (AT-MSCs) with
            lysomustine has a superior antitumor effect in the lung carcinoma
            model compared to monotherapies with transfected AT-MSCs or
            lysomustine alone.
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Merkwirth,C., Jovaisaite,V., Durieux,J., Matilainen,O.,
            Jordan,S.D., Quiros,P.M., Steffen,K.K., Williams,E.G.,
            Mouchiroud,L., Tronnes,S.U., Murillo,V., Wolff,S.C., Shaw,R.J.,
            Auwerx,J. and Dillin,A.
  TITLE     Two Conserved Histone Demethylases Regulate Mitochondrial
            Stress-Induced Longevity
  JOURNAL   Cell 165 (5), 1209-1223 (2016)
   PUBMED   27133168
REFERENCE   3  (bases 1 to 7052)
  AUTHORS   Xing,L., Sun,X., Deng,X., Kotedia,K., Urano,M., Koutcher,J.A.,
            Ling,C.C. and Li,G.C.
  TITLE     Expression of the bifunctional suicide gene CDUPRT increases
            radiosensitization and bystander effect of 5-FC in prostate cancer
            cells
  JOURNAL   Radiother Oncol 92 (3), 345-352 (2009)
   PUBMED   19433338
  REMARK    GeneRIF: CDUPRT-expressing cells were more sensitive to 5-FC and
            5-FU than CD-expressing cells. CDUPRT-expression further enhanced
            the radiosensitizing effect of 5-FC.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731717.11.
            
            On Dec 5, 2019 this sequence version replaced NM_001081189.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.341256.1,
                                           SRR10223971.18898.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-547               AL731717.11        49063-49609
            548-590             AL731717.11        61996-62038
            591-660             AL731717.11        64535-64604
            661-723             AL731717.11        65664-65726
            724-885             AL731717.11        68005-68166
            886-984             AL731717.11        70589-70687
            985-7052            AL731717.11        72416-78483
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X"
     gene            1..7052
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /note="uracil phosphoribosyltransferase"
                     /db_xref="GeneID:331487"
                     /db_xref="MGI:MGI:2685620"
     exon            1..547
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            548..590
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            591..660
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            661..723
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            724..885
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            886..984
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     exon            985..7052
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7025..7030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /note="hexamer: ATTAAA"
     regulatory      7030..7035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /note="hexamer: AATAAA"
     polyA_site      7045
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /note="major polyA site"
     polyA_site      7052
                     /gene="Uprt"
                     /gene_synonym="D830012I24Rik; Gm774"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgaaagg accgtagagg cctaggggtg agagggcagc cagggttaga gatttggagg
       61 agccaggggc caggaggacg cacgtgtccg catccttttc ttcttgccct ttatccctag
      121 ggtttgatag cagcggggat agctcggggg ccccgtgtat ggcctcggag ttacagcgtc
      181 cggactccat gccctgtcac aatcggcaag taaactctac ttctagccca agtcccgagc
      241 atctgctagc cgaggaccgg gtcctggatc atgcagagga aaataacgct gctatggcta
      301 agctgactct cctccctggg cacgcccatt ctagcgtgct ttcggagcgg gactctccgg
      361 cctgctgcag cactaatctt cactctgaga accacagtga cagtagtgac agtggcaact
      421 acgatgcacc tgtcggcggc gactccctgc taggggactg tgaactctcc cgacagattg
      481 gggctcagct taagttgctg cctatgaatg atcagatccg ggagcttcag actatcatcc
      541 gggacaagac agccagtaga ggggacttca tgttttctgc agatcgcttg atcagacttg
      601 ttgtagaaga gggactgaat cagctgccat ataaagaatg tatggtgacc actccgacag
      661 ggcacaagta tgaaggagtg aaatttgaga aaggaaattg tggggtcagc ataatgagaa
      721 gtggtgaggc aatggaacaa ggtttgcgag actgctgtcg atccatacgg attgggaaga
      781 tcctgattca gagtgatgag gagacacaaa gggccaaagt atattatgcc aagttccccc
      841 cagacattca tcgcagaaaa gtccttctga tgtatccaat tctcagtact ggaaatactg
      901 taattgaagc tgtaaaggtt cttatagaac atggtgttca acccagtgtt attatcctac
      961 tcagtctctt ctccacccca catggtgcca aatcaatcat tcaagaattt ccagagatca
     1021 caattttaac tacagaagtc catcctgttg cacctacaca ttttggacag aaatactttg
     1081 gaacagacta agttcttaaa ggaaaatgaa ataccttaca taatattatg gttataaatg
     1141 gattccatat ctgtttttac taatttgttt gagaaaatat gttaggaata ccttttaaca
     1201 ttggaagtag gtgtagcaac aagaaatatt ggaagtttat ttatttgatt tgatcatttc
     1261 ttcacatgtg gtaccaaatt caacaatgaa gcacaacctc agtgcttaaa accgatggaa
     1321 attttaattg tatgctaata gaagtcactg actcttcagt tagtccatgg agactgtaga
     1381 acttaggacc ccatctcttg gtttggaagt tgcttgtagc tgccaagaaa acccaaatga
     1441 gagttagttc tttggtacac tgcaggttta cactgtcctt actgtgtttt agctctagag
     1501 tgtttccctg tatgtgggtc ttgtcaccct ctgtactcta tatactatat atatatacac
     1561 atatacatat atatatatgt acatatatat atgtacacac acacacacac acacacacac
     1621 acacacacac acacacacac acacacacac acacacatgc aggtaccttg aagaagagag
     1681 aatgagagta tatcccggaa ttttatgttg ccatttatgt cctcacttaa tttgcaggcc
     1741 caagatgtcc gcttaacttg tctgctaagc cgttgtattc ctcaaggctg gctcatttcc
     1801 ccaagtggga attgagggaa ggtacttacc aagctgtaac atggtgacaa agacgcaggg
     1861 tcttttcagt aactgaatct gtaacatgag tctacagaga ctaaaccacc gatgcctgat
     1921 gctgacccaa gagagctcaa tcagccttct acaaaggaat tggtgtagat ttttctaaga
     1981 tgtgggggaa agtagaggct ttgcttttcg tggccaataa ggaaatcaaa acggttcttt
     2041 ggttgttgtt gtttttcaag gtagggtttc tctgtgttcc tcgggctgtc ctggaactca
     2101 ctgtgtagac caggttggcc ttgaactcat agagatccac ctgcttctgc ctcccaagtt
     2161 ctgggattaa aggcatgcag cacaacacaa tatgcaatac ggttcttttt aatttagttg
     2221 tttctattac ttgcttttgg tttggaactt agttattttg catgtcttat gactatacct
     2281 gtattaaagt agagaataca taaagtcttt taaaatatat aaggtttgta tgtgttcata
     2341 tttaagcttc tgaaaggaga ggaaacaatt ttagaatcaa tctttgcact tttgaaatgt
     2401 gcttataaaa cccagattcc tgttttacat ggatattgtg gtggctgggt gattacttga
     2461 ttaattacac tctcatggcc ctgcagagag ctgctgtggg gctctgttga aaagtataag
     2521 aaaaagtaca taggaatttc ttagaactga agctgaaaag gtcagtgaag ctcaagggag
     2581 gaaagcaacc tggaggaaat tgactggact ttaccttatg gttctagcct actaaacttc
     2641 actgtactgt tgttgttgtt gttgtttctt tgaggcagag cttctctgtg tagtcctggc
     2701 tatcctgaaa catgctctga agaccaggct ggcctccaac tcagagatcc accaacctct
     2761 gccttccaag tgctaagatt aaatgtgtgt gccaccatgc ccagctactt tgtttttggt
     2821 tgtgagccta gccttcaatg gctgagccat ctctccagcc ctatttttga agactattat
     2881 gtaataaaat taaaattcat caaacagttc cttccatagt agaatgttgc cttcaagttc
     2941 ttcctgaaat ttttttgaga tagggtctcc tggctggctt gtaacacata gacatcatcc
     3001 tgcctcttga gtgctgggat taaagttgtg ctgaaagtta tatttatatt tgatattagg
     3061 tgtcctaaga atgatgtgag tgcaaattca gatataacgg atctgttgac attgttattg
     3121 cactgaatca gtagaaaaga caatggatgc tcactaacat gggtgtggta ggaggatttc
     3181 aactttgagg ctagtgtaag taactgagtc agatcttgtc tcaaaaattt tgttttgccc
     3241 actaacctaa gaattgctgt gactcaattg aattggtgct tgtcttggct gcagtattat
     3301 ctttgaatat gagataattt caaactccac tgtagggata aaaccatatt ttaagcaaac
     3361 tcttttttaa attttgaatc atgtcttcgt acataggcta gctttgaact tgctgtgtag
     3421 tcaactctga tcttgaactc ctgttctcac tgcctccact gacttctggt atgaaaggct
     3481 taccaccagc atgccaattt aaaacaaatt atttttctac cattagccat ttagattcaa
     3541 atgtctgttg cttttcagca ataaatgtga ttaaaagtta actacatttg attctcacat
     3601 ggaagcttag tataaacagt aggaattatt caataaggtc atattccctt ctgatgtttg
     3661 taccctacaa ctcctgcaga tataactctg gtctctgaat tttggaatgc tttagcaaac
     3721 aaattcgtac ttgcagggct aatcacttgc aggactctat tttctaaagt caacataaga
     3781 ttaacagact attaaaagta agattgactt tagtgacatg aacttcagag cagtataaat
     3841 tgtgcaggtt ctttattgaa aaatagaagc tagagcaaaa tgatgtgcaa ataacacaaa
     3901 gctgtgattc tcaaagtatg gcatacatca taattattcc caatgatgtg ctacagattg
     3961 ctgaacccca aatttacagt gtctgattta atgtcttgat ttccaacatt gattcttttt
     4021 ataatgtagg cctttgatga tataaaactt tccataagga tttagctgat ttttgatatg
     4081 tttccatttt gattttcttt tatatttctt caacccaggg tcatttagaa gcatcaacca
     4141 actgcaaata ttcacaccat tttccatatt tctctttact attgtttcct aatttaactc
     4201 catggaggtc agagaatatg attgtcatga tttgaattct ttcaatttgt gagatttttt
     4261 agacaaaggc tcttgtccat aaactattgt tatgtggtaa gcctccttcc taccacttgt
     4321 tctactgatt attaggagaa ggatattgaa aactcagatt aaaagtgttg tatttatttc
     4381 ttctttcaat tctatgaagg ttttgctttg tgtattttga agcactcttg ttaggtgtat
     4441 acgctgttag ggtggctgta gtctcttgat gatatgacct gttaatactc tttgtgctga
     4501 aatcccagat ttcttttgag tcgatttact tttaacatgg ttatgtttta atgtgaattt
     4561 attatagtca ggtttggagc tgtgtcttgc tttttttgat cctttttttt aattagatat
     4621 tttcttcatt tacatttcaa atgctatccc aaaagtcccc cataccctcc cccccccact
     4681 cccctaccca cccactccca cttcttggcc ctggagttcc cctgtactgg ggtatataaa
     4741 gtttgcaata ccaaggggcc tctcttccca ttgatgtgtc ttgcttgtta aaaaatccat
     4801 tttgacagtg tcagcttttt gttgcagtgt ttagataatt tttgtgtcat gtaattattg
     4861 acatgattgg ttttagatct accatatggc tatttccttc tatatttact tttttttcat
     4921 tttcatcttt ttgattatta tttatgattc tattttatgt cttcatgatg tttgtgtcag
     4981 ctctttccac tgaatcctcc aggatcttgg cccaaactct ttctctctct ctattatata
     5041 gctctagctg tcctgaaatt tactgtgtag actaggctgc ccttgaactt gtagtgccag
     5101 gactaaaggc atgtaccacc aacttggcca gcctagtctc ccttaaagga agacttctgt
     5161 atttttctga gcatttacat ttaatatagc agttgtacaa cagacaaatt atagaaataa
     5221 tagcatgagt atatctgacg tttggaggat tctatgcaaa atatagactt gaagcagtta
     5281 atcatttata aagccattat atacatctca aatattttat attaatctga ttattttccc
     5341 tttgtctacc attatttgac cattatgaca agctcatatg ggactgatta tcatattaat
     5401 ttgttaactt catctagctt ctcatatcag tcattttcct ttgatatcac atctcaaaga
     5461 agcgtaaata aagttgctca atacatataa ttatcagtgc attatttgac ctgtttaata
     5521 tacttgaatt ttaccagcat ttctaacctt ccaccatatc acacaaatac tgcatggtta
     5581 gtttattcta caggtcactc agataaccat tatactttgc ccccatataa ttggctcttg
     5641 accaagtgcc agatattgtt tagggaaagg tgatgttata gttgagcaag atttgtttat
     5701 ttccaggttt caaatcaata ttttagttta ttcgagctgt tgtaaaaata ccagtgtagg
     5761 tgttttgttt tgttttgttt tgttgtttct ctgtgtaaca gtcctggctg ttgtggaact
     5821 agtttagtag accaggctgt cctcaaactt agagagatct gcctgcctct acctcctggg
     5881 tgctgggatt aaggacatag accaccactc ctggcaggac tggttcttat ggcaaatcta
     5941 agcatgtagt ttagaattat ttctacgtct gctgtaaaat caagtacaac ttcctttgct
     6001 ctgttcttgg catgagggca ggcagtgcat attagtgtaa gctggttttg ccttcagaga
     6061 atttgaagtt tggaggctga gtttggaggt ttaaaggaat tctgcaagtg ttgatgtgcc
     6121 attgtctatc tcgaagggct attgattcat atctggggca ggctccaact gttttaaatt
     6181 ggtttaaaga ccctatttgt tcatggcttt gcccgactgt gagtactaac actctccagc
     6241 tttattcaag gaatctgcct gtcccaggtt gctaaagcct taccaggctg attcctgtct
     6301 tctggaagag tgatctttct actgatttaa tctacttaaa ttaccatttt cttctgaatg
     6361 tcattcatta aaaggaaatc tcttttgctc tagcagtcac actactgagt atgtaagcag
     6421 agagaatgaa attaccatgc tgaagagaca tctgcactcc tgagttcatt gaagcagtat
     6481 tttcattaat taaaaactag aaacagccaa agtgtacatc acatgataaa tgtataaaga
     6541 gaggatggtg catttatatg ataggttact actctgccat acaaaggaat gaaatcctgt
     6601 cttttgtgac acaatcagat gtaactggag attatgttaa gtgagtgaaa caagccagtc
     6661 acagattaca ccatatgatt tctgctcatg tagaatccag tgaaaaatga tctcatacaa
     6721 gttgttacca aaagctgtgg gggagatgcc caaatgggaa aaggttgaac aagggtcact
     6781 aagttacagt tacttagaag aaagttctgc tgtgctatta catacttgtg tgtctgtaga
     6841 tcatcattaa tgtactatac atgaaaatta gaagaaaata ttttgttaag tttttactat
     6901 gaagaagtga taatgaggaa agagataaac atgatttaaa tattatacaa tatgtacatg
     6961 tactgaaata tatgatatct cattaatatg tacaaagttt ctttttatgg ctcacttaaa
     7021 ataaattaaa ataaattaaa aataataact ga
//
