LOCUS       NM_009474               1843 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus urate oxidase (Uox), mRNA.
ACCESSION   NM_009474
VERSION     NM_009474.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Chang,H., Tao,Q., Wei,L., Wang,Y. and Tu,C.
  TITLE     Spatiotemporal landscape of kidney in a mouse model of
            hyperuricemia at single-cell level
  JOURNAL   FASEB J 39 (2), e70292 (2025)
   PUBMED   39817712
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1843)
  AUTHORS   Pang,L., Liang,N., Li,C., Merriman,T.R., Zhang,H., Yan,F., Sun,W.,
            Li,R., Xue,X., Liu,Z., Wang,C., Cheng,X., Chen,S., Yin,H.,
            Dalbeth,N. and Yuan,X.
  TITLE     A stable liver-specific urate oxidase gene knockout hyperuricemia
            mouse model finds activated hepatic de novo purine biosynthesis and
            urate nephropathy
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (3), 167009 (2024)
   PUBMED   38237409
  REMARK    GeneRIF: A stable liver-specific urate oxidase gene knockout
            hyperuricemia mouse model finds activated hepatic de novo purine
            biosynthesis and urate nephropathy.
REFERENCE   4  (bases 1 to 1843)
  AUTHORS   Liu,Y., Jarman,J.B., Low,Y.S., Augustijn,H.E., Huang,S., Chen,H.,
            DeFeo,M.E., Sekiba,K., Hou,B.H., Meng,X., Weakley,A.M.,
            Cabrera,A.V., Zhou,Z., van Wezel,G., Medema,M.H., Ganesan,C.,
            Pao,A.C., Gombar,S. and Dodd,D.
  TITLE     A widely distributed gene cluster compensates for uricase loss in
            hominids
  JOURNAL   Cell 186 (16), 3400-3413 (2023)
   PUBMED   37541197
  REMARK    Erratum:[Cell. 2023 Sep 28;186(20):4472-4473. doi:
            10.1016/j.cell.2023.08.036. PMID: 37774682]
REFERENCE   5  (bases 1 to 1843)
  AUTHORS   Toyoda,Y., Miyata,H., Uchida,N., Morimoto,K., Shigesawa,R.,
            Kassai,H., Nakao,K., Tomioka,N.H., Matsuo,H., Ichida,K.,
            Hosoyamada,M., Aiba,A., Suzuki,H. and Takada,T.
  TITLE     Vitamin C transporter SVCT1 serves a physiological role as a urate
            importer: functional analyses and in vivo investigations
  JOURNAL   Pflugers Arch 475 (4), 489-504 (2023)
   PUBMED   36749388
REFERENCE   6  (bases 1 to 1843)
  AUTHORS   Cook,S., Johnson,K. and Davisson,M.
  TITLE     The mouse urate oxidase gene, Uox, maps to distal chromosome 3
  JOURNAL   Mamm Genome 8 (8), 623-624 (1997)
   PUBMED   9250879
REFERENCE   7  (bases 1 to 1843)
  AUTHORS   Wu,X., Wakamiya,M., Vaishnav,S., Geske,R., Montgomery,C. Jr.,
            Jones,P., Bradley,A. and Caskey,C.T.
  TITLE     Hyperuricemia and urate nephropathy in urate oxidase-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 742-746 (1994)
   PUBMED   8290593
REFERENCE   8  (bases 1 to 1843)
  AUTHORS   Wu,X.W., Lee,C.C., Muzny,D.M. and Caskey,C.T.
  TITLE     Urate oxidase: primary structure and evolutionary implications
  JOURNAL   Proc Natl Acad Sci U S A 86 (23), 9412-9416 (1989)
   PUBMED   2594778
REFERENCE   9  (bases 1 to 1843)
  AUTHORS   Hemsley,A., Pegg,M., Crane,D. and Masters,C.
  TITLE     On the compartmentalization of catalase, fatty acyl-CoA oxidase and
            urate oxidase in mammalian livers, and the influence of clofibrate
            treatment on this microlocalization
  JOURNAL   Mol Cell Biochem 83 (2), 187-194 (1988)
   PUBMED   3200253
REFERENCE   10 (bases 1 to 1843)
  AUTHORS   Lee,P.C.
  TITLE     Developmental changes of adenosine deaminase, xanthine oxidase, and
            uricase in mouse tissues
  JOURNAL   Dev Biol 31 (2), 227-233 (1973)
   PUBMED   4207016
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC141632.4.
            
            On Apr 27, 2007 this sequence version replaced NM_009474.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050425.1, BC019771.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AC141632.4         34626-34710         c
            86-304              AC141632.4         21371-21589         c
            305-421             AC141632.4         19460-19576         c
            422-500             AC141632.4         19288-19366         c
            501-684             AC141632.4         7212-7395           c
            685-808             AC141632.4         5981-6104           c
            809-892             AC141632.4         3975-4058           c
            893-1843            AC141632.4         376-1326            c
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 72.09 cM"
     gene            1..1843
                     /gene="Uox"
                     /note="urate oxidase"
                     /db_xref="GeneID:22262"
                     /db_xref="MGI:MGI:98907"
     exon            1..85
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..967
                     /gene="Uox"
                     /EC_number="1.7.3.3"
                     /codon_start=1
                     /product="uricase"
                     /protein_id="NP_033500.1"
                     /db_xref="CCDS:CCDS17905.1"
                     /db_xref="GeneID:22262"
                     /db_xref="MGI:MGI:98907"
                     /translation="MAHYHDNYGKNDEVEFVRTGYGKDMVKVLHIQRDGKYHSIKEVA
                     TSVQLTLRSKKDYLHGDNSDIIPTDTIKNTVHVLAKLRGIRNIETFAMNICEHFLSSF
                     NHVTRAHVYVEEVPWKRFEKNGIKHVHAFIHTPTGTHFCEVEQMRNGPPVIHSGIKDL
                     KVLKTTQSGFEGFLKDQFTTLPEVKDRCFATQVYCKWRYQRRDVDFEAIWGAVRDIVL
                     QKFAGPYDKGEYSPSVQKTLYDIQVLSLSQLPEIEDMEISLPNIHYFNIDMSKMGLIN
                     KEEVLLPLDNPYGKITGTVKRKLPSRL"
     exon            86..304
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            305..421
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            422..500
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            501..684
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            685..808
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            809..892
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1843
                     /gene="Uox"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1741..1746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uox"
                     /note="hexamer: AATAAA"
     polyA_site      1769
                     /gene="Uox"
                     /note="major polyA site"
     regulatory      1798..1803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uox"
                     /note="hexamer: AATAAA"
     polyA_site      1823
                     /gene="Uox"
     regulatory      1826..1831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uox"
                     /note="hexamer: AACAAA"
     polyA_site      1842
                     /gene="Uox"
ORIGIN      
        1 acaccattcc gtctcattct tgaaatcgaa cctgaagtta aaggctgagg gaaaaatggc
       61 ccattaccat gacaactacg gaaagaatga tgaagtggaa tttgtccgaa ctggctatgg
      121 gaaagacatg gtcaaagttc tccatattca gagagatgga aaataccaca gcatcaaaga
      181 ggtggcaact tcggtccagt tgactctgag gtccaagaag gattacctac acggtgataa
      241 ttccgacatc atccctacag acaccatcaa gaacactgtg cacgtcctgg cgaagctcag
      301 agggatcaga aacatcgaga cctttgcaat gaacatctgt gagcacttcc tctcttcttt
      361 taaccatgtc actcgagccc acgtctacgt ggaagaggtc ccctggaaac gatttgaaaa
      421 gaatgggatc aaacacgtcc atgcattcat ccacaccccg acgggaacgc acttctgtga
      481 ggtggagcag atgagaaacg gacctcccgt cattcactct ggaatcaaag acctcaaggt
      541 cttgaaaaca acccagtctg ggtttgaagg gttcctcaag gaccagttca ctaccctccc
      601 tgaggtgaag gaccgatgct ttgccactca agtgtactgc aagtggcgct accagagacg
      661 ggacgtggac ttcgaggcta tctggggcgc tgtccgggac attgtcctgc agaaatttgc
      721 tgggccctat gacaaaggtg aatactcacc ttccgtgcag aagaccctct atgatataca
      781 agtgctgtcc ctgagccagc ttcctgagat agaagacatg gaaatcagcc ttccgaacat
      841 tcactacttt aacatcgaca tgtccaaaat gggactgatc aacaaggaag aggttttgct
      901 gcctctcgac aatccctacg gcaaaataac agggacagtc aagaggaagc tgccttccag
      961 gctgtgacgt catggctcca gccggttgcg atgctgggga atactgtatg cttgcctgca
     1021 aaaatttcaa ctcaggatat tgattgcttt gttctgtatg aagtaattgc tttgttgtta
     1081 ttgttgttcc tagttcaaca gggaacttgt agtggtatgt atgtggagtc ccatgttagc
     1141 aggcccaacc tgctccacgg ccatgggcag tttccttaaa aattactggg ccatgttagg
     1201 agaatcacag tgatagttct ccttgctgtt cttgtgttct ctgcctttga atccgtcttt
     1261 agaatccaat tgtcataagg acataatcgt aaagtactgg ctgccgccac attgattaat
     1321 ctgaacacag aagcaaacac tgtcatcatc ctagggatat gtctgggtct aactgaaaac
     1381 agaataaaac catgtgaaaa aaaaagtcac cataatgttt tttaaaaact ctggcactct
     1441 gactagcact cacatggagg aaggcgggtg ggcatgaaac tccaatccta gctcaggagt
     1501 tattggtgtt tgattgaggc tggaagaggg aaagtcagct tcatttaggg gtgtgacacc
     1561 tggtatgtca atcatactga aggacaccag gttgcatgcc tagaagtggt ccgataaacc
     1621 caaactggcc tcagtgggaa agagaaagaa catgaagttg ggtgagtgag aaagacttgg
     1681 gataaactgg gatgggaagt gaatattatg aaacttattg tataaaattc tcaaataatt
     1741 aataaaaaca ttattttaaa tgtttaaaag atacttttaa gttcctctat cagcacaaat
     1801 aaagataaga gcatgaaaac aaacaaacaa acaaaaacaa aac
//
