LOCUS       NM_011677               1906 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus uracil DNA glycosylase (Ung), transcript variant 2,
            mRNA.
ACCESSION   NM_011677
VERSION     NM_011677.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Hayran,A.B., Liabakk,N.B., Aas,P.A., Kusnierczyk,A., Vagbo,C.B.,
            Sarno,A., Iveland,T.S., Chawla,K., Zahn,A., Di Noia,J.M.,
            Slupphaug,G. and Kavli,B.
  TITLE     RPA guides UNG to uracil in ssDNA to facilitate antibody class
            switching and repair of mutagenic uracil at the replication fork
  JOURNAL   Nucleic Acids Res 52 (2), 784-800 (2024)
   PUBMED   38000394
  REMARK    GeneRIF: RPA guides UNG to uracil in ssDNA to facilitate antibody
            class switching and repair of mutagenic uracil at the replication
            fork.
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Dickinson,K., Hammond,L., Akpa,M., Chu,L.L., Lalonde,C.T.,
            Goumba,A. and Goodyer,P.
  TITLE     WT1 regulates expression of DNA repair gene Neil3 during
            nephrogenesis
  JOURNAL   Am J Physiol Renal Physiol 324 (3), F245-F255 (2023)
   PUBMED   36546838
REFERENCE   3  (bases 1 to 1906)
  AUTHORS   Martin,O.A., Thomas,M., Marquet,M., Bruzeau,C., Garot,A.,
            Brousse,M., Bender,S., Carrion,C., Choi,J.E., Vuong,B.Q.,
            Gearhart,P.J., Maul,R.W., Le Noir,S. and Pinaud,E.
  TITLE     The IgH Emicro-MAR regions promote UNG-dependent error-prone repair
            to optimize somatic hypermutation
  JOURNAL   Front Immunol 14, 1030813 (2023)
   PUBMED   36865553
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1906)
  AUTHORS   Feng,Y., Li,C., Stewart,J.A., Barbulescu,P., Seija Desivo,N.,
            Alvarez-Quilon,A., Pezo,R.C., Perera,M.L.W., Chan,K., Tong,A.H.Y.,
            Mohamad-Ramshan,R., Berru,M., Nakib,D., Li,G., Kardar,G.A.,
            Carlyle,J.R., Moffat,J., Durocher,D., Di Noia,J.M., Bhagwat,A.S.
            and Martin,A.
  TITLE     FAM72A antagonizes UNG2 to promote mutagenic repair during antibody
            maturation
  JOURNAL   Nature 600 (7888), 324-328 (2021)
   PUBMED   34819670
  REMARK    GeneRIF: FAM72A antagonizes UNG2 to promote mutagenic repair during
            antibody maturation.
REFERENCE   5  (bases 1 to 1906)
  AUTHORS   Delgado,P., Alvarez-Prado,A.F., Marina-Zarate,E., Sernandez,I.V.,
            Mur,S.M., de la Barrera,J., Sanchez-Cabo,F., Canamero,M., de
            Molina,A., Belver,L., de Yebenes,V.G. and Ramiro,A.R.
  TITLE     Interplay between UNG and AID governs intratumoral heterogeneity in
            mature B cell lymphoma
  JOURNAL   PLoS Genet 16 (12), e1008960 (2020)
   PUBMED   33362210
  REMARK    GeneRIF: Interplay between UNG and AID governs intratumoral
            heterogeneity in mature B cell lymphoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1906)
  AUTHORS   Krokan,H.E., Otterlei,M., Nilsen,H., Kavli,B., Skorpen,F.,
            Andersen,S., Skjelbred,C., Akbari,M., Aas,P.A. and Slupphaug,G.
  TITLE     Properties and functions of human uracil-DNA glycosylase from the
            UNG gene
  JOURNAL   Prog Nucleic Acid Res Mol Biol 68, 365-386 (2001)
   PUBMED   11554311
  REMARK    Review article
REFERENCE   7  (bases 1 to 1906)
  AUTHORS   Nilsen,H., Steinsbekk,K.S., Otterlei,M., Slupphaug,G., Aas,P.A. and
            Krokan,H.E.
  TITLE     Analysis of uracil-DNA glycosylases from the murine Ung gene
            reveals differential expression in tissues and in embryonic
            development and a subcellular sorting pattern that differs from the
            human homologues
  JOURNAL   Nucleic Acids Res 28 (12), 2277-2285 (2000)
   PUBMED   10871356
REFERENCE   8  (bases 1 to 1906)
  AUTHORS   Nilsen,H., Rosewell,I., Robins,P., Skjelbred,C.F., Andersen,S.,
            Slupphaug,G., Daly,G., Krokan,H.E., Lindahl,T. and Barnes,D.E.
  TITLE     Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role
            of the enzyme during DNA replication
  JOURNAL   Mol Cell 5 (6), 1059-1065 (2000)
   PUBMED   10912000
REFERENCE   9  (bases 1 to 1906)
  AUTHORS   Svendsen,P.C., Yee,H.A., Winkfein,R.J. and van de Sande,J.H.
  TITLE     The mouse uracil-DNA glycosylase gene: isolation of cDNA and
            genomic clones and mapping ung to mouse chromosome 5
  JOURNAL   Gene 189 (2), 175-181 (1997)
   PUBMED   9168124
REFERENCE   10 (bases 1 to 1906)
  AUTHORS   Nilsen,H., Otterlei,M., Haug,T., Solum,K., Nagelhus,T.A.,
            Skorpen,F. and Krokan,H.E.
  TITLE     Nuclear and mitochondrial uracil-DNA glycosylases are generated by
            alternative splicing and transcription from different positions in
            the UNG gene
  JOURNAL   Nucleic Acids Res 25 (4), 750-755 (1997)
   PUBMED   9016624
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129080.6, AK168709.1 and BB400166.1.
            
            On May 25, 2006 this sequence version replaced NM_011677.1.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5'region, lacks a portion of the 5' coding region, and initiates
            translation at an alternate start codon, compared to variant 1. The
            encoded isoform (b) has a distinct N-terminus and is shorter than
            isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X99018.1, U55041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               AC129080.6         154162-154519
            359-1899            AK168709.1         309-1849
            1900-1906           BB400166.1         277-283
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.97 cM"
     gene            1..1906
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /note="uracil DNA glycosylase"
                     /db_xref="GeneID:22256"
                     /db_xref="MGI:MGI:109352"
     exon            1..358
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..961
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /EC_number="3.2.2.27"
                     /note="isoform b is encoded by transcript variant 2; UDG"
                     /codon_start=1
                     /product="uracil-DNA glycosylase isoform b"
                     /protein_id="NP_035807.2"
                     /db_xref="CCDS:CCDS19560.1"
                     /db_xref="GeneID:22256"
                     /db_xref="MGI:MGI:109352"
                     /translation="MGVLGRRSLRLARRAGLRSLTPNPDSDSRQASPAKKARVEQNEQ
                     GSPLSAEQLVRIQRNKAAALLRLAARNVPAGFGESWKQQLCGEFGKPYFVKLMGFVAE
                     ERNHHKVYPPPEQVFTWTQMCDIRDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPS
                     LENIFKELSTDIDGFVHPGHGDLSGWARQGVLLLNAVLTVRAHQANSHKERGWEQFTD
                     AVVSWLNQNLSGLVFLLWGSYAQKKGSVIDRKRHHVLQTAHPSPLSVHRGFLGCRHFS
                     KANELLQKSGKKPINWKEL"
     exon            359..454
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     exon            455..552
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     exon            553..641
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     exon            642..820
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1906
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1885..1890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /note="hexamer: AATAAA"
     polyA_site      1905
                     /gene="Ung"
                     /gene_synonym="UNG1; UNG2"
                     /note="major polyA site"
ORIGIN      
        1 gggaggtgtt ttgccgcgaa aagcctgcgt gggagcgcag acgggtgcag cgggtcccct
       61 gctcggctgg accatgggcg tcttggggcg gcggtctttg cggttggcgc ggagagcggg
      121 gctccggagc ctgactccca accccgactc tgactcccgg caggccagcc ccgccaagaa
      181 ggcccgggtg gagcagaacg agcaaggctc gccgctcagc gccgagcagc tcgtccgcat
      241 ccagaggaac aaggccgcgg cgctgctcag gctcgccgcc cgcaacgtgc ctgccggctt
      301 cggcgagagc tggaagcagc agctgtgcgg ggagttcggg aagccgtact tcgtcaagct
      361 aatgggattt gttgctgaag aaaggaacca ccacaaggtc tatccgcccc cggagcaggt
      421 gttcacatgg acccagatgt gcgacatccg agatgtgaag gttgtcattc tgggacagga
      481 tccctatcac ggacctaatc aagctcacgg gctctgcttc agtgtccaaa gaccagttcc
      541 acccccgccc agtttggaaa acatttttaa agagctgtct acagacatcg atggttttgt
      601 tcatcctggc catggggatt tgtcagggtg ggcccgacaa ggtgtcctcc tcctcaacgc
      661 cgtcctcact gtccgcgccc accaagccaa ttcccataag gagaggggct gggagcagtt
      721 cacggacgcg gtcgtgtcct ggctgaatca gaacctgagt ggcctcgtct tcctcctctg
      781 gggctcttat gctcagaaga agggcagtgt cattgacagg aagcgtcacc atgtcctgca
      841 gacagctcac ccctccccgc tgtcggtgca cagagggttc ctgggatgca gacatttttc
      901 taaagccaat gaactgctcc agaagtctgg caagaagccc atcaactgga aggagctgtg
      961 acccgcccac ccgcccacag gccgcatctg tccacagcgg tgctttcagc tgccgccgca
     1021 gcttttgctg gttaagttct aagttccttg tgtgagtgga gaaatgacac ggatagatct
     1081 ctataggacc atcatgagcc aaccaggaca cagaaactgc agctctggca ggcagaacct
     1141 cttgcctggc actaggtttg aactctccag gcccggccag gtggctgttg agacacacct
     1201 acttctcaga ccagcggaca caccctcggt cccgtgtgct gctctggggt gggaggaaag
     1261 atctgcacct ttggaccagc agtttggtgc ctgctgctgt ccgctttatc ctttagagtt
     1321 agagatttca aagtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     1381 gcttcaaaag gcccttttgt ctggaaagga acccttgggt gttggtgagt cctgagaagt
     1441 ctggcttaca actgaatgag cttcctgatc acaaaggggg cggggttctc cctgggttca
     1501 cagtggccgc tctccaccct gcgtcttgcc tttgcctttg caggctccga agttgagcga
     1561 agcaggaatg ggatagattt gtttttaagt gagggtgggt gggtttcctg gtggcggtgg
     1621 agttgtttcc tcctctctgc agacagcaga ctgccgcgct tggtgtgtgt ggtgctgggg
     1681 atctaactcc aaggtgctgt ttcttagggg ctgagttcac acaagttgtc tttctgcaac
     1741 actggagaac gttatttttt tttacaaggt aggcaggtac agtaatttct ttttcatgct
     1801 tttctgaaag acaatcttca ctgagtttac aagctggatt ccgtgagtcc agctggttag
     1861 agccgtggcc tgcctgacca ggctaataaa agatggttga caattg
//
