LOCUS       NM_178680               3571 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus unc-45 myosin chaperone B (Unc45b), transcript variant
            1, mRNA.
ACCESSION   NM_178680 XM_913527
VERSION     NM_178680.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Loo,L., Simon,J.M., Xing,L., McCoy,E.S., Niehaus,J.K., Guo,J.,
            Anton,E.S. and Zylka,M.J.
  TITLE     Single-cell transcriptomic analysis of mouse neocortical
            development
  JOURNAL   Nat Commun 10 (1), 134 (2019)
   PUBMED   30635555
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3571)
  AUTHORS   Wiederstein,J.L., Nolte,H., Gunther,S., Piller,T., Baraldo,M.,
            Kostin,S., Bloch,W., Schindler,N., Sandri,M., Blaauw,B., Braun,T.,
            Holper,S. and Kruger,M.
  TITLE     Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates
            p97 and Regulates Autophagy-Associated Protein Breakdown
  JOURNAL   Cell Rep 23 (5), 1342-1356 (2018)
   PUBMED   29719249
REFERENCE   4  (bases 1 to 3571)
  AUTHORS   Nicholls,P., Bujalowski,P.J., Epstein,H.F., Boehning,D.F.,
            Barral,J.M. and Oberhauser,A.F.
  TITLE     Chaperone-mediated reversible inhibition of the sarcomeric myosin
            power stroke
  JOURNAL   FEBS Lett 588 (21), 3977-3981 (2014)
   PUBMED   25240199
  REMARK    GeneRIF: UNC-45B and Hsp90 involved in sarcomere function
REFERENCE   5  (bases 1 to 3571)
  AUTHORS   Uribe,V., Badia-Careaga,C., Casanova,J.C., Dominguez,J.N., de la
            Pompa,J.L. and Sanz-Ezquerro,J.J.
  TITLE     Arid3b is essential for second heart field cell deployment and
            heart patterning
  JOURNAL   Development 141 (21), 4168-4181 (2014)
   PUBMED   25336743
REFERENCE   6  (bases 1 to 3571)
  AUTHORS   Srikakulam,R., Liu,L. and Winkelmann,D.A.
  TITLE     Unc45b forms a cytosolic complex with Hsp90 and targets the
            unfolded myosin motor domain
  JOURNAL   PLoS One 3 (5), e2137 (2008)
   PUBMED   18478096
  REMARK    GeneRIF: mammalian Unc45b is a cytosolic protein that forms a
            stable complex with Hsp90, selectively binds the unfolded
            conformation of the myosin motor domain, and promotes motor domain
            folding
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3571)
  AUTHORS   Liu,L., Srikakulam,R. and Winkelmann,D.A.
  TITLE     Unc45 activates Hsp90-dependent folding of the myosin motor domain
  JOURNAL   J Biol Chem 283 (19), 13185-13193 (2008)
   PUBMED   18326487
  REMARK    GeneRIF: vertebrate Unc45 isoforms clearly demonstrates a direct
            role for Unc45 in Hsp90-mediated myosin motor domain folding and
            highlights major differences between the isoforms in substrate
            specificity and mechanism
REFERENCE   8  (bases 1 to 3571)
  AUTHORS   Augustin,M., Sedlmeier,R., Peters,T., Huffstadt,U., Kochmann,E.,
            Simon,D., Schoniger,M., Garke-Mayerthaler,S., Laufs,J., Mayhaus,M.,
            Franke,S., Klose,M., Graupner,A., Kurzmann,M., Zinser,C., Wolf,A.,
            Voelkel,M., Kellner,M., Kilian,M., Seelig,S., Koppius,A.,
            Teubner,A., Korthaus,D., Nehls,M. and Wattler,S.
  TITLE     Efficient and fast targeted production of murine models based on
            ENU mutagenesis
  JOURNAL   Mamm Genome 16 (6), 405-413 (2005)
   PUBMED   16075367
REFERENCE   9  (bases 1 to 3571)
  AUTHORS   Price,M.G., Landsverk,M.L., Barral,J.M. and Epstein,H.F.
  TITLE     Two mammalian UNC-45 isoforms are related to distinct cytoskeletal
            and muscle-specific functions
  JOURNAL   J Cell Sci 115 (Pt 21), 4013-4023 (2002)
   PUBMED   12356907
REFERENCE   10 (bases 1 to 3571)
  AUTHORS   Le Bras,S., Cohen-Tannoudji,M., Guyot,V., Vandormael-Pournin,S.,
            Coumailleau,F., Babinet,C. and Baldacci,P.
  TITLE     Transcript map of the Ovum mutant (Om) locus: isolation by exon
            trapping of new candidate genes for the DDK syndrome
  JOURNAL   Gene 296 (1-2), 75-86 (2002)
   PUBMED   12383505
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603745.16.
            
            On Dec 21, 2022 this sequence version replaced NM_178680.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK084418.1, AK168968.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                AL603745.16        27464-27537
            75-242              AL603745.16        27858-28025
            243-279             AL603745.16        28573-28609
            280-455             AL603745.16        29382-29557
            456-545             AL603745.16        31228-31317
            546-713             AL603745.16        33210-33377
            714-882             AL603745.16        33907-34075
            883-1053            AL603745.16        35902-36072
            1054-1225           AL603745.16        38926-39097
            1226-1526           AL603745.16        42065-42365
            1527-1621           AL603745.16        44833-44927
            1622-1763           AL603745.16        45110-45251
            1764-1904           AL603745.16        45599-45739
            1905-2032           AL603745.16        50010-50137
            2033-2099           AL603745.16        50923-50989
            2100-2213           AL603745.16        52552-52665
            2214-2329           AL603745.16        53002-53117
            2330-2447           AL603745.16        53914-54031
            2448-2603           AL603745.16        56367-56522
            2604-3571           AL603745.16        58669-59636
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 50.3 cM"
     gene            1..3571
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="unc-45 myosin chaperone B"
                     /db_xref="GeneID:217012"
                     /db_xref="MGI:MGI:2443377"
     exon            1..74
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..2864
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cardiomyopathy associated 4; striated muscle UNC45;
                     protein unc-45 homolog B"
                     /codon_start=1
                     /product="protein unc-45 homolog B isoform 1"
                     /protein_id="NP_848795.3"
                     /db_xref="CCDS:CCDS36251.1"
                     /db_xref="GeneID:217012"
                     /db_xref="MGI:MGI:2443377"
                     /translation="MAEAEAAQLKEEGNRHFQLQDYKAATKSYSQALKLTKDKALLAT
                     LYRNRAACGLKMESYAQAASDASRAIDINSADIKALYRRCQALEHLGKLDQAFKDVQR
                     CATLEPRNQNFQETLRRLNTSIQEQLRVQFSTDSRVQTMFEILLNENSEADKREKAAN
                     NLIVLGREEAGAERIFQSNGVALLLQLMNTQRPELLLAAVRTLSGMCSGHRARATAIL
                     HAVRIDRICSLMALENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLK
                     QITSHLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLSIHDNSRTIYVVDNGLRKILKV
                     VGQVPDLPSCLPLTDNTRMLASILINKLYDDLRCDPERDHFRKICEEYITSKFDPQDM
                     DKNVNAIQTVSGILQGPFDLGNQLLGMKGVMEMMVALCGSEREADQLVAVEALIHAST
                     KLSRATFIITNGVTLLKQIYKTTKNEKIKIRTLVGLCKLGSAGGSDYGLRQFAEGSTE
                     KLAKQCRKWLCNTAIDTRTRRWAVEGLAYLTLDADVKDDFVQDIPALQAMFELAKTSD
                     KTILYSVANTLVNCTNSYDVKEVVPELVQLAKFSKQHVPEEHPKDKKDFVDLRVKRLL
                     KAGVISALACMVKADSAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLAL
                     EGTDVGKVKAAHGLAKIAAVSNPDIAFPGERVYEVVRPLVSLLDTQRDGLQNYEALLG
                     LTNLSGRSDKLRQKIFKEKALPDIENYMFENHDQLRQAATECMCNMVLNKEVQERFLA
                     DGNDRLKLVVLLCGEDDHKLQNAAAGALAMLTAAHKKLCLKMTEVTTQWLEILQRLCL
                     HDQLSVQHRGLVIAHNLLSADAELARKLVESELLEILTVVGKQEPDEKRAAVVQTARE
                     CLIKCMDYGFIKPVS"
     exon            75..242
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            243..279
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            280..455
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            456..545
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            546..713
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            714..882
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1053
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1225
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1526
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1621
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1763
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1904
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2032
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2099
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2213
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2329
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2447
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2603
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..3571
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3231..3236
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="hexamer: CATAAA"
     polyA_site      3259
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="major polyA site"
     regulatory      3485..3490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="hexamer: AATAAA"
     regulatory      3495..3500
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
                     /note="hexamer: AATGAA"
     polyA_site      3519
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
     polyA_site      3543
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
     polyA_site      3571
                     /gene="Unc45b"
                     /gene_synonym="Cmya4; D230041A13Rik; UNC-45B; Unc45"
ORIGIN      
        1 agcagatcaa gctgtctgag gtggaccgca gagctcagag cagtgcctag gaggaggcat
       61 cgtgcagaaa gactatggca gaggctgaag cggcacagct gaaggaggag gggaaccggc
      121 attttcagct ccaggattac aaggcggcca ccaagagcta cagccaggcc ctgaagctga
      181 ccaaggacaa ggctctgcta gctacactct accggaatcg ggcagcctgc ggcctgaaaa
      241 tggagagcta cgcccaggca gcctcggatg cttccagagc cattgacatc aactctgctg
      301 acatcaaagc attgtatcgg cgatgccagg cactggagca tcttggcaag ttggaccagg
      361 cattcaagga tgtgcagcgc tgtgctaccc tggagccaag gaaccagaac ttccaggaga
      421 cactgcggag gctcaacacc agcatccaag agcagctccg tgtgcagttt tctacagact
      481 ccagggtaca gacgatgttt gaaattcttc tgaacgaaaa cagcgaggct gataagcgag
      541 agaaggctgc caacaacctc attgtcctgg gccgtgaaga agcgggtgct gagaggatct
      601 tccagagcaa tggagtggct ctgctgctgc agcttatgaa cacccagcgg cctgagctgc
      661 tgctagcggc tgtgcggacg ctgtctggca tgtgcagtgg ccacagagct agggccaccg
      721 ccatcctgca cgcagtccgc atagacagaa tctgcagcct catggccctg gagaatgaag
      781 agatgtccct ggctgtctgc aacctgctcc aggccatcat tgactccctg tctggagagg
      841 acaagagaga gcatcgtggg aaggaggagg ccttggttct agataccaag aaggacctga
      901 agcagatcac cagccacctg ctggacatgc tggtcagtaa gaaagtgtct gggcagggca
      961 gagaccaggc actgaacctg cttaataaga atgtccccag gaaggacctg tccattcatg
     1021 acaactcacg caccatctat gttgtagata acggtctgag gaagatcctg aaggtggtgg
     1081 ggcaggttcc agacctgccc tcctgcctac ccctgacgga caacacccgc atgctggcct
     1141 ccatcctcat caacaagctc tatgatgacc tgcgctgtga cccagagcgc gaccacttcc
     1201 gcaagatctg tgaggagtat atcacgagca aatttgaccc ccaggacatg gacaagaatg
     1261 taaatgccat ccagacggtg tccggcatct tgcaggggcc ctttgacctg ggcaatcagc
     1321 tgctgggcat gaaaggtgtg atggagatga tggtggctct gtgtggctcg gagcgcgagg
     1381 cagaccagct ggtggctgtg gaggctctca tccacgcctc cacgaagctc agccgggcca
     1441 ccttcatcat caccaacgga gtgacacttc ttaagcagat ctacaagacc acaaagaatg
     1501 agaagatcaa gatccgcacg ctggtggggc tctgtaaact tggctcagct ggaggctccg
     1561 actatggtct cagacagttt gctgaagggt caacagagaa gctagccaag cagtgtcgaa
     1621 agtggctgtg taacaccgcc atagacaccc ggacgcggcg ctgggcagtg gagggcctgg
     1681 cctacctcac gctggacgct gacgtgaagg acgactttgt ccaggacatc cctgctctgc
     1741 aggccatgtt tgagttggcc aagaccagtg acaagaccat cctctactca gtggccaaca
     1801 ccctggtaaa ctgcaccaac agctatgatg tcaaggaggt cgtccctgag ctggtgcagc
     1861 tggccaagtt ctccaagcaa catgtgccag aggagcaccc caaggacaag aaggacttcg
     1921 ttgacttgcg ggtgaaacgt cttctgaagg caggtgtcat atctgcactg gcctgcatgg
     1981 tgaaggcaga cagtgccatc ctcacagacc agaccaagga gctgctggcc agggtattcc
     2041 tggcactctg tgacaatcca aaggaccggg gcaccattgt agctcaaggt ggtggcaagg
     2101 ccctgatccc cctggctttg gagggcacag atgtgggcaa ggtgaaggca gcccatgggc
     2161 tagccaagat tgctgctgtc tccaacccgg acattgcctt ccctggggag agggtatatg
     2221 aggtggtgcg gcccctcgtg agtctcctgg acacacagag ggatgggctt cagaactatg
     2281 aggctctgct gggactgact aacctgtctg ggcgcagtga caaactcagg cagaagatct
     2341 tcaaggagaa ggccttgcca gacattgaaa actacatgtt tgagaaccat gaccagctac
     2401 ggcaggcagc caccgaatgc atgtgtaaca tggtgctcaa taaggaggtt caggagaggt
     2461 tcctggccga tgggaatgat cgtctgaagc tggtggtgct gctctgtggg gaggatgacc
     2521 acaaactgca gaacgcagca gctggagctc tagccatgct gaccgcggca cataagaaac
     2581 tgtgcctcaa gatgacggag gtgacaaccc agtggctgga gatcctacag cggctttgcc
     2641 tgcatgacca gttgtccgtg cagcaccggg gcctggtcat tgcccacaac ctgctatcag
     2701 cggatgctga gctggcgagg aagctggtgg agagcgagct gctggagatc ctgactgtgg
     2761 tgggaaagca ggagccagat gagaagagag cggctgtggt ccagacagcc cgggagtgtc
     2821 ttatcaagtg catggattat ggcttcatca agccggtgtc atagagagtg gtgcacaggg
     2881 atgctggggt tggtcctgaa ctgtgaagag tcgtgagctg ggtcctccca aagtgtcagt
     2941 tctgctaagg atcacagcag tgccaatgat gtctcattac aaagcaaaga cttggttgtc
     3001 tcctgatttg tgactcttcc ttttggtcct aggaaagttt gagtgttcct gcctccctca
     3061 atttgtcaca tcccagaaat gtgtaggata agttctctct gtgatcagaa agcaggtcag
     3121 acaattctgc aggccagata acgggctgta gactgatttt tgtttgtttg tttgttttgt
     3181 attaaaaaaa aactgtgtag tggcaaggaa ggaattaaca ggtgtgtgac cataaagatc
     3241 aatgctgttg tttaattaag atgcggatgt ctattgattt tcataacctc tcctacactt
     3301 caaaactatg tgtctcaact gcatgtgccc tgtccaagcc ccatgcccac ccctgccacc
     3361 cgatttcaat ggtaacacag tttatgctca cagtgggacg tggcccattt cagaaagtct
     3421 ttcatgtgag atggatatgg ccactaccaa taagatcaac tctaagtccc ctgagcagaa
     3481 cataaataaa gattaatgaa tatgtgttag tcacccacag agaggtctga cttgctagat
     3541 ccagctccgt tcaatgtcat taccagtctt a
//
