LOCUS       NM_133952               3285 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus unc-45 myosin chaperone A (Unc45a), mRNA.
ACCESSION   NM_133952
VERSION     NM_133952.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Clemente,V., Hoshino,A., Meints,J., Shetty,M., Starr,T., Lee,M. and
            Bazzaro,M.
  TITLE     UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse
            Genital Tract and in the Microtubule-Rich Areas of the Mouse
            Nervous System
  JOURNAL   Cells 10 (7), 1604 (2021)
   PUBMED   34206743
  REMARK    GeneRIF: UNC-45A Is Highly Expressed in the Proliferative Cells of
            the Mouse Genital Tract and in the Microtubule-Rich Areas of the
            Mouse Nervous System.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3285)
  AUTHORS   Jiang,F., Takagi,Y., Shams,A., Heissler,S.M., Friedman,T.B.,
            Sellers,J.R. and Bird,J.E.
  TITLE     The ATPase mechanism of myosin 15, the molecular motor mutated in
            DFNB3 human deafness
  JOURNAL   J Biol Chem 296, 100243 (2021)
   PUBMED   33372036
  REMARK    GeneRIF: The ATPase mechanism of myosin 15, the molecular motor
            mutated in DFNB3 human deafness.
REFERENCE   4  (bases 1 to 3285)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 3285)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3285)
  AUTHORS   Lamberti,A., Sanges,C., Chambery,A., Migliaccio,N., Rosso,F., Di
            Maro,A., Papale,F., Marra,M., Parente,A., Caraglia,M.,
            Abbruzzese,A. and Arcari,P.
  TITLE     Analysis of interaction partners for eukaryotic translation
            elongation factor 1A M-domain by functional proteomics
  JOURNAL   Biochimie 93 (10), 1738-1746 (2011)
   PUBMED   21689717
REFERENCE   7  (bases 1 to 3285)
  AUTHORS   Liu,L., Srikakulam,R. and Winkelmann,D.A.
  TITLE     Unc45 activates Hsp90-dependent folding of the myosin motor domain
  JOURNAL   J Biol Chem 283 (19), 13185-13193 (2008)
   PUBMED   18326487
REFERENCE   8  (bases 1 to 3285)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 3285)
  AUTHORS   Price,M.G., Landsverk,M.L., Barral,J.M. and Epstein,H.F.
  TITLE     Two mammalian UNC-45 isoforms are related to distinct cytoskeletal
            and muscle-specific functions
  JOURNAL   J Cell Sci 115 (Pt 21), 4013-4023 (2002)
   PUBMED   12356907
REFERENCE   10 (bases 1 to 3285)
  AUTHORS   Yanai,N., Sato,Y. and Obinata,M.
  TITLE     A new type-II membrane protein in erythropoietic organs enhances
            erythropoiesis
  JOURNAL   Leukemia 11 Suppl 3, 484-485 (1997)
   PUBMED   9209433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB842393.1, AK166809.1 and CJ323134.1.
            
            On Apr 28, 2009 this sequence version replaced NM_133952.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC004717.1, AK166809.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BB842393.1         1-18
            19-3259             AK166809.1         2-3242
            3260-3285           CJ323134.1         425-450
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 45.65 cM"
     gene            1..3285
                     /gene="Unc45a"
                     /note="unc-45 myosin chaperone A"
                     /db_xref="GeneID:101869"
                     /db_xref="MGI:MGI:2142246"
     exon            1..151
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..2935
                     /gene="Unc45a"
                     /note="smooth muscle cell associated protein-1; SMAP-1;
                     UNC-45A; stromal membrane-associated protein 1; unc-45
                     homolog A"
                     /codon_start=1
                     /product="protein unc-45 homolog A"
                     /protein_id="NP_598713.2"
                     /db_xref="CCDS:CCDS21395.1"
                     /db_xref="GeneID:101869"
                     /db_xref="MGI:MGI:2142246"
                     /translation="MTVSGPETPEPRPSDPGASSAEQLRKEGNELFKCGDYEGALTAY
                     TQALSLGATPQDQAILHRNRAACHLKLEDYSKAESEASKAIEKDGGDVKALYRRSQAL
                     EKLGRLDQAVLDLKRCVSLEPKNKVFQESLRNIGGQIQEKVRYMSSTDAKVEQMFQIL
                     LDPKEKGTEKKQKASQNLVVLAREDAGAEKIFRSNGVQLLQRLLDTEETDLMLAALRT
                     LVGICSEHQSRTVATLSVLGTRRVVSILGVENQAVSLAACHLLQVIFDALKEGVKKGF
                     RGKEGAIIVDPARELKVLINSLLELLTEVGVSGQGRDNALTLLIKMVPRKSPKDPNNS
                     LTLWVIDQGLKKILEVGGSLQDAAGELTVTANSRMSASILLSKLFDDLKCDAERENFH
                     RLCENYIRNWFEGHGLAGKLRAIQTVSCLLQGPCDAGNRALELSGVMESVIALCASER
                     EEEQLVAVEALIHAAGKAKRASFITANGVSLLKDLYKGSERDSIRIRALVGLCKLGSA
                     GGTDFSMKQFAEGSTLKLAKQCRKWLCNDQIDAGTRRWAVEGLAYLTFDADVKEEFVE
                     DEAALKALFQLSRSEERSVLFAVGSALVNCTNSYDYEEPDPKMVELAKYAKQHVPEQH
                     PKDKPSFVRARVKKLLAAGVVSAMTCMVKTESPVLTNSCRELLSRVFLALVEEVEDRG
                     TVVAQGGGKALLPLALEGTDVGQTKAAQALAKLTITSNPEMTFPGERIYEVVRPLVSL
                     LHLSCSGLQNFEALMALTNLAGISERLRQKILKEKAVPMIEGYMFEEHEMIRRAATEC
                     MCNLAMSKEVQDLFEAQGNDRLKLLVLYSGEDDELLRRAAAGGLAMLTSMRPALCSRI
                     PQVTTHWLEILQALLLSPNQELQHRGTVVVLNMMQSSKEIAGTLMESEVLEILSVLAK
                     GEESPVTRAAAACLEKAVEYRLIQPNQDGE"
     exon            152..313
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            314..350
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            351..526
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            527..619
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            620..787
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            788..956
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1127
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1299
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1600
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1695
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1837
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1978
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2106
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2173
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2287
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2403
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2521
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2677
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..3263
                     /gene="Unc45a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3243..3248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Unc45a"
                     /note="hexamer: AATAAA"
     polyA_site      3263
                     /gene="Unc45a"
                     /note="major polyA site"
ORIGIN      
        1 gcattgctgg ggcggggcag tcgccggcat ctcgcggatc ccagggacac ctttgctcta
       61 aagactgcgc ctgcactggc tcttggagct tagcttcgtg atgactgtga gtggcccgga
      121 gacccccgag ccccgacctt ccgaccccgg ggccagctca gccgagcagc tgcggaagga
      181 gggcaacgag ctgttcaaat gcggggatta cgagggcgcc ctgaccgcct acacacaggc
      241 cctgagtctc ggcgcgacgc cccaggacca ggccattttg caccgaaaca gggctgcttg
      301 ccacctcaag ctggaagatt acagcaaagc agaatcagag gcgtctaaag ccattgaaaa
      361 ggacggtggg gatgtcaaag cactctatcg ccgaagccaa gccctagaga agctgggccg
      421 cctggaccag gctgtccttg acctgaagag atgtgtgagc ctagaaccca agaacaaagt
      481 tttccaggag tcgctgagga acattggggg ccagattcag gagaaggtgc ggtacatgtc
      541 ctccacagat gccaaagtag aacagatgtt tcagatactg ttggacccca aagagaaagg
      601 cacggagaag aagcagaagg cttctcagaa ccttgtggta ctggcccgag aggatgctgg
      661 agctgagaag atcttccgga gcaacggggt tcagctcttg cagcgcctgc tggacacaga
      721 agagactgac ctcatgctgg cagccctgcg cacgctggtc ggcatttgct ccgagcacca
      781 gtcgcggaca gtggcaaccc tgagtgtcct gggaactcgg agagtcgtct ccatcctggg
      841 tgtggaaaac caggccgtgt cgctggcagc ttgccacctg ctgcaggtta tctttgatgc
      901 cctcaaggaa ggcgtcaaga aaggcttccg aggcaaagaa ggtgctatta tcgtggatcc
      961 tgcccgggag ctgaaggttc tcatcaatag cctcctggag ctactgaccg aggtgggggt
     1021 ctctggccaa ggccgcgaca atgccctgac cctcctcatt aaaatggtac cccggaaatc
     1081 accaaaggac cccaacaata gcctcacact ctgggtcatt gatcaaggtc tgaagaagat
     1141 tctggaggtg ggcggctccc tgcaggatgc tgctggtgag ctcacggtga cggcaaacag
     1201 ccgcatgagt gcctccatcc tcctgagcaa gctgtttgat gatctgaagt gtgatgccga
     1261 gcgagaaaac ttccaccgac tctgtgagaa ctacatcagg aactggtttg agggccatgg
     1321 gctggccggg aagctacgag ccatccagac agtgtcctgc cttctgcagg gcccatgcga
     1381 cgctggcaac cgggccttag agctgagcgg tgtcatggag agcgtgatag ctctgtgcgc
     1441 ctccgagcgg gaggaagagc agctggtagc cgtggaggca ctgatccacg ctgccggcaa
     1501 agccaagcgg gcctcgttca tcactgccaa cggtgtctct ctgctcaagg acctgtacaa
     1561 gggcagcgag agggacagca tccgcatcag agcactagtg ggactctgta agctcggatc
     1621 ggctggaggg actgacttca gcatgaagca gtttgctgaa ggctctaccc tcaaactggc
     1681 caagcagtgc cgaaagtggc tatgcaatga ccagattgat gcaggtactc ggcgctgggc
     1741 agtggagggc ctggcttacc tcacctttga tgcggacgtg aaggaagagt ttgtggagga
     1801 cgaggctgct ctgaaggccc tgttccagct aagcaggtct gaagaaaggt cagtgctctt
     1861 tgccgtaggc tcggcattgg tcaattgcac caacagctac gactatgagg agcctgaccc
     1921 caagatggtg gagctggcca agtatgccaa gcagcacgtt ccagaacagc accccaagga
     1981 caaaccaagc ttcgtgcggg ctcgtgtgaa gaagctgctg gcggcgggtg tggtgtcagc
     2041 catgacgtgt atggtgaaga cggagagccc cgtgctgacg aactcctgta gggagctact
     2101 ctccagggtc ttcctggctt tggtggaaga ggtggaggat cgaggtactg tggttgccca
     2161 gggagggggc aaggctctgc tccccctggc tctggaaggc actgatgttg ggcagaccaa
     2221 ggccgcccag gctcttgcca agctgaccat tacctccaac ccagagatga cctttcctgg
     2281 cgagcggatc tacgaagtag tccggcccct tgtctctctg ctgcacctca gctgctcagg
     2341 actgcagaac tttgaggccc tcatggccct aacaaacctg gcagggatca gtgagaggct
     2401 caggcagaag atcctgaagg agaaggctgt gcccatgatt gagggctaca tgtttgagga
     2461 gcatgagatg atccgccgcg cagctaccga gtgcatgtgt aacttagcca tgagcaaaga
     2521 ggtgcaagat ctcttcgagg cccagggcaa tgaccggctg aagctgctgg tgctgtacag
     2581 cggcgaggat gatgagctgc tgcggagggc ggctgctggg ggcctggcca tgctcacctc
     2641 catgcggccc gcactctgca gtcgtatccc tcaagtgacc acacactggc tggagatctt
     2701 gcaggccctg ctcctcagcc ccaaccagga gctgcagcac cggggcaccg tggtggtgct
     2761 gaacatgatg cagtcttcga aggagatcgc cggcaccctg atggagagtg aggtcctgga
     2821 gatcctgtca gtgttggcca agggcgagga gagcccagtc accagggctg ccgcagcctg
     2881 cctggagaaa gcagtggaat atagactcat ccaacccaac caggacggag agtgacgatc
     2941 ggcccggggc ccacagctca cgcatctgtc acccttcaca gctctgagtc agcactggga
     3001 tgctcgatac ttgcctcgcc tccacagctg ccacagttgc actttggtgt tctcaggaca
     3061 cacagcatac agccctgccc tgccagcact caggagggcc ctgtctgtac tgtagagagc
     3121 tgaggctggg ggacgtacat cccattcaag cctgaggatt atgggcatca agaaggaagg
     3181 acacaacagc atcctcacat gctgggagca tcatcagagc ctgccagcag gctcaccccg
     3241 ctaataaatt ctgtggaact aaaaaaaaaa aaaaaaaaaa aaaaa
//
