LOCUS       NM_001278605            2581 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus uromodulin (Umod), transcript variant 2, mRNA.
ACCESSION   NM_001278605
VERSION     NM_001278605.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Hamilton,A.D.K., Sparsoe,L.V., Skov,M., Johnsen,N.,
            Chreistensen,M.H., Corydon,T.J. and Praetorius,H.
  TITLE     Increased water intake dilutes protective uromodulin levels in
            urine and results in increased rates of pyelonephritis in a murine
            model
  JOURNAL   Acta Physiol (Oxf) 240 (9), e14204 (2024)
   PUBMED   39007512
  REMARK    GeneRIF: Increased water intake dilutes protective uromodulin
            levels in urine and results in increased rates of pyelonephritis in
            a murine model.
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Yano-Sakamoto,K., Kitai,Y., Toriu,N., Yamamoto,S., Mizuta,K.,
            Saitou,M., Tsukiyama,T., Taniuchi,I., Osato,M. and Yanagita,M.
  TITLE     Expression pattern of Runt-related transcription factor (RUNX)
            family members and the role of RUNX1 during kidney development
  JOURNAL   Biochem Biophys Res Commun 722, 150155 (2024)
   PUBMED   38795454
REFERENCE   3  (bases 1 to 2581)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2581)
  AUTHORS   Santambrogio,S., Cattaneo,A., Bernascone,I., Schwend,T., Jovine,L.,
            Bachi,A. and Rampoldi,L.
  TITLE     Urinary uromodulin carries an intact ZP domain generated by a
            conserved C-terminal proteolytic cleavage
  JOURNAL   Biochem Biophys Res Commun 370 (3), 410-413 (2008)
   PUBMED   18375198
  REMARK    GeneRIF: Urinary uromodulin is generated by a conserved C-terminal
            proteolytic cleavage and retains its entire ZP domain.
REFERENCE   5  (bases 1 to 2581)
  AUTHORS   Mo,L., Huang,H.Y., Zhu,X.H., Shapiro,E., Hasty,D.L. and Wu,X.R.
  TITLE     Tamm-Horsfall protein is a critical renal defense factor protecting
            against calcium oxalate crystal formation
  JOURNAL   Kidney Int 66 (3), 1159-1166 (2004)
   PUBMED   15327412
  REMARK    GeneRIF: THP is a critical urinary defense factor and its
            deficiency could be an important contributing factor in
            nephrolithiasis
REFERENCE   6  (bases 1 to 2581)
  AUTHORS   Mo,L., Zhu,X.H., Huang,H.Y., Shapiro,E., Hasty,D.L. and Wu,X.R.
  TITLE     Ablation of the Tamm-Horsfall protein gene increases susceptibility
            of mice to bladder colonization by type 1-fimbriated Escherichia
            coli
  JOURNAL   Am J Physiol Renal Physiol 286 (4), F795-F802 (2004)
   PUBMED   14665435
  REMARK    GeneRIF: potential THP defects, either quantitative or qualitative,
            could predispose the urinary bladder to bacterial infections
REFERENCE   7  (bases 1 to 2581)
  AUTHORS   Bates,J.M., Raffi,H.M., Prasadan,K., Mascarenhas,R., Laszik,Z.,
            Maeda,N., Hultgren,S.J. and Kumar,S.
  TITLE     Tamm-Horsfall protein knockout mice are more prone to urinary tract
            infection: rapid communication
  JOURNAL   Kidney Int 65 (3), 791-797 (2004)
   PUBMED   14871399
  REMARK    GeneRIF: THP serves as a soluble receptor for type 1 fimbriated E.
            coli and helps eliminate bacteria from the urinary tract.
REFERENCE   8  (bases 1 to 2581)
  AUTHORS   Prasadan,K., Bates,J., Badgett,A., Dell,M., Sukhatme,V., Yu,H. and
            Kumar,S.
  TITLE     Nucleotide sequence and peptide motifs of mouse uromodulin
            (Tamm-Horsfall protein)--the most abundant protein in mammalian
            urine
  JOURNAL   Biochim Biophys Acta 1260 (3), 328-332 (1995)
   PUBMED   7873609
REFERENCE   9  (bases 1 to 2581)
  AUTHORS   Sainio,K., Gilbert,S.F., Lehtonen,E., Nishi,M., Kumar,N.M.,
            Gilula,N.B. and Saxen,L.
  TITLE     Differential expression of gap junction mRNAs and proteins in the
            developing murine kidney and in experimentally induced nephric
            mesenchymes
  JOURNAL   Development 115 (3), 827-837 (1992)
   PUBMED   1425357
REFERENCE   10 (bases 1 to 2581)
  AUTHORS   Klein,G., Langegger,M., Goridis,C. and Ekblom,P.
  TITLE     Neural cell adhesion molecules during embryonic induction and
            development of the kidney
  JOURNAL   Development 102 (4), 749-761 (1988)
   PUBMED   3048972
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB843740.1, AK165507.1 and
            AC125147.4.
            
            Summary: This gene encodes a glycoprotein that is the most abundant
            protein in mammalian urine under physiological conditions. It is
            synthesized in the kidney as a glycosyl-phosphatidylinositol
            anchored protein and released into urine as a soluble form by
            proteolytic cleavage. It is thought to regulate water and salt
            balance in the thick ascending limb of Henle and to protect against
            urinary tract infection and calcium oxalate crystal formation. In
            mouse deficiency of this gene is associated with increased
            susceptibility to bacterial infections and formation of calcium
            crystals in kidneys. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165507.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               BB843740.1         2-378
            378-1161            AK165507.1         294-1077
            1162-1162           AC125147.4         83552-83552         c
            1163-2578           AK165507.1         1079-2494
            2579-2581           AC125147.4         73762-73764         c
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.88 cM"
     gene            1..2581
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /note="uromodulin"
                     /db_xref="GeneID:22242"
                     /db_xref="MGI:MGI:102674"
     exon            1..111
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            112..231
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..2072
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /note="uromucoid; Tamm-Horsfall urinary glycoprotein"
                     /codon_start=1
                     /product="uromodulin preproprotein"
                     /protein_id="NP_001265534.1"
                     /db_xref="CCDS:CCDS21780.1"
                     /db_xref="GeneID:22242"
                     /db_xref="MGI:MGI:102674"
                     /translation="MGIPLTWMLLVMMVTSWFTLAEASNSTEARRCSECHNNATCTVD
                     GVVTTCSCQTGFTGDGLVCEDMDECATPWTHNCSNSSCVNTPGSFKCSCQDGFRLTPE
                     LSCTDVDECSEQGLSNCHALATCVNTEGDYLCVCPEGFTGDGWYCECSPGSCEPGLDC
                     LPQGPDGKLVCQDPCNTYETLTEYWRSTEYGVGYSCDAGLHGWYRFTGQGGVRMAETC
                     VPVLRCNTAAPMWLNGSHPSSSEGIVSRTACAHWSDQCCRWSTEIQVKACPGGFYIYN
                     LTAPPECNLAYCTDPSSVEGTCEECRVDEDCISDNGRWRCQCKQDSNITDVSQLEYRL
                     ECGANDIKMSLRKCQLQSLGFMNVFMYLNDRQCSGFSESDERDWMSIVTPARNGPCGT
                     VLRRNETHATYSNTLYLANAIIIRDIIIRMNFECSYPLDMKVSLKTSLQPMVSALNIS
                     LGGTGKFTVRMALFQSPTYTQPHQGPSVMLSTEAFLYVGTMLDGGDLSRFVLLMTNCY
                     ATPSSNSTDPVKYFIIQDSCPRTEDTTIQVTENGESSQARFSVQMFRFAGNYDLVYLH
                     CEVYLCDSTSEQCKPTCSGTRFRSGNFIDQTRVLNLGPITRQGVQASVSKAASSNLRL
                     LSIWLLLFPSATLIFMVQ"
     exon            232..1011
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1119
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1328
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1477
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1723
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1886
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1968
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2010
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2581
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2557..2562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /note="hexamer: AATAAA"
     polyA_site      2576
                     /gene="Umod"
                     /gene_synonym="THP; Urehd1; urehr4"
                     /note="major polyA site"
ORIGIN      
        1 agagggcaga agcagagaat aatctgaggg agagggccag ccaagggcag gcaagtatat
       61 attgatcaca ggcacttact tgtgaatgga ccagtcctgt cctgggttca ggtgtttcca
      121 ggacagaggg cagagtgtaa aggatgggga tccctttgac ctggatgctg ctggtaatga
      181 tggtaacctc ctggttcact ctggctgaag ccagtaactc aacagaagcg agacggtgtt
      241 ctgaatgcca caacaacgcc acctgcacgg tggatggtgt ggtcacaacg tgctcctgcc
      301 agaccggctt cactggtgat gggctggtgt gtgaggacat ggatgagtgt gctaccccat
      361 ggactcacaa ctgctccaac agcagctgtg tgaacacccc gggctcgttt aagtgctcct
      421 gtcaggatgg ttttcgtctg acgcctgagc tgagctgcac tgatgtggat gagtgctcag
      481 agcaggggct cagtaactgt catgccctgg ccacctgtgt caacacagaa ggcgactact
      541 tgtgcgtgtg tcccgagggc tttacagggg atggttggta ctgtgagtgc tccccaggct
      601 cctgtgagcc aggactggac tgcttgcccc agggcccgga tggaaagctg gtgtgtcaag
      661 acccctgcaa tacatatgag accctgactg agtactggcg cagcacagag tatggtgtgg
      721 gctactcctg tgacgcgggt ctgcacggct ggtaccggtt cacaggccag ggtggcgttc
      781 gcatggctga gacctgtgtg cccgtcctgc gatgcaacac ggcggcaccc atgtggctca
      841 atggctctca tccctcgagt agtgaaggca ttgtgagccg cacggcctgt gcacactgga
      901 gcgaccaatg ctgccggtgg tccacagaga tccaggtgaa ggcttgccca ggtggcttct
      961 atatttacaa cttgacagcg ccccctgagt gcaatctggc ttactgcacc gatcctagtt
     1021 ccgtggaggg gacttgcgaa gaatgcaggg tagatgaaga ttgcatatcg gataacggca
     1081 gatggcgctg ccagtgtaaa caggactcca acatcacaga tgtctcccaa ttggagtaca
     1141 ggctggagtg tggggccaat gacatcaaga tgtccctcag aaagtgccag ctacagagtt
     1201 tgggctttat gaatgtcttc atgtacctga atgacagaca atgctcaggc ttcagtgaga
     1261 gtgatgaacg agactggatg tccatagtga cccctgccag gaatggtccc tgtgggacag
     1321 tattgaggag aaacgaaacc catgccacct acagcaacac cctctacctg gcaaatgcga
     1381 tcatcattcg ggacatcatc ataagaatga actttgaatg ctcttaccct ctggacatga
     1441 aagtcagcct gaagacctcc ctacagccca tggtcagtgc cctgaacatc agcttgggtg
     1501 ggacaggcaa gttcaccgtg cggatggcat tgttccagag ccctacctac acacagcccc
     1561 accaaggtcc ttctgtgatg ctgtccactg aggcttttct gtatgtgggc accatgctgg
     1621 atgggggtga cttgtcccgg tttgtactgc taatgaccaa ctgctatgcc acacccagta
     1681 gcaactccac agaccctgtg aaatacttca ttatccagga cagttgtcca cgtacagaag
     1741 atacaaccat tcaggtgaca gagaatggcg agtcatctca ggcccgattt tctgttcaga
     1801 tgttccggtt tgcaggaaac tacgaccttg tctaccttca ctgcgaggtg tacctatgtg
     1861 actctacgag tgaacagtgt aaacctacct gctctggtac tagatttcga agtgggaact
     1921 tcatagatca gacccgtgtc ctgaacttgg gtcccataac acgacaaggt gtccaggcct
     1981 cagtgtccaa ggctgcttcc agcaacttga ggctcctgag catctggctg ctgttgtttc
     2041 cctcagccac tttgatcttc atggttcaat gatggaaagc agaaaacctg gtgtggctcc
     2101 cagctcactt cctgctggcc aggagtgggg atgcaggctg gttatcagcc aggagagagg
     2161 gagctcacac tgcctccagc ttgcacgtac tctttcttta atcctcacca tcaaaaacaa
     2221 gttggcatct ttcagtgctg ttcttccttc aaatgtgata tgtatgaagc ccctgtctcc
     2281 tgaaggaatg tggcaaaata ataagtttaa agtatcagct tttggactca ttaattcaag
     2341 tcgttacaga acacacagtt gctgtgagca ctgtctgttg tgctgggcat tacatggtgg
     2401 gaataaaaca gatcctttct ctatctttat ggacttcata gatgctctaa gaaactaggc
     2461 tcaatgcatg ttctgctatg tgatctaaga tgagacacac aatgtatatg aacttcacct
     2521 gtctatccat tcatgtatac atccatcctt tcactcaata aacatttgcc aagtcactaa
     2581 t
//
