LOCUS       NM_001355512            5327 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 2,
            mRNA.
ACCESSION   NM_001355512 XM_006538203
VERSION     NM_001355512.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   He,C., Xing,X., Chen,H.Y., Gao,M., Shi,J., Xiang,B., Xiao,X.,
            Sun,Y., Yu,H., Xu,G., Yao,Y., Xie,Z., Xing,Y., Budiarto,B.R.,
            Chen,S.Y., Gao,Y., Lee,Y.R. and Zhang,J.
  TITLE     UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance
            anti-tumor immunity
  JOURNAL   Mol Cell 84 (6), 1120-1138 (2024)
   PUBMED   38377992
  REMARK    GeneRIF: UFL1 ablation in T cells suppresses PD-1 UFMylation to
            enhance anti-tumor immunity.
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5327)
  AUTHORS   Chen,F., Sheng,L., Zhou,T., Yan,L., Loveless,R., Li,H., Teng,Y. and
            Cai,Y.
  TITLE     Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological
            damage and carcinogenesis through activating mTOR signaling
  JOURNAL   J Exp Clin Cancer Res 42 (1), 110 (2023)
   PUBMED   37131258
  REMARK    GeneRIF: Loss of Ufl1/Ufbp1 in hepatocytes promotes liver
            pathological damage and carcinogenesis through activating mTOR
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5327)
  AUTHORS   Zhou,J., Ma,X., He,X., Chen,B., Yuan,J., Jin,Z., Li,L., Wang,Z.,
            Xiao,Q., Cai,Y., Zou,Y. and Cong,Y.S.
  TITLE     Dysregulation of PD-L1 by UFMylation imparts tumor immune evasion
            and identified as a potential therapeutic target
  JOURNAL   Proc Natl Acad Sci U S A 120 (11), e2215732120 (2023)
   PUBMED   36893266
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2023 May 23;120(21):e2306087120.
            doi: 10.1073/pnas.2306087120.. PMID: 37186868]
REFERENCE   5  (bases 1 to 5327)
  AUTHORS   Wang,K., Xu,H.N., Wang,Y.W., Mao,J., Liu,D., Zhu,X.J., Cong,Y.S.
            and Wang,M.
  TITLE     Ufl1 deficiency causes skin pigmentation by up-regulation of
            Endothelin-1
  JOURNAL   Front Cell Dev Biol 10, 961675 (2022)
   PUBMED   36120581
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5327)
  AUTHORS   Shiwaku,H., Yoshimura,N., Tamura,T., Sone,M., Ogishima,S.,
            Watase,K., Tagawa,K. and Okazawa,H.
  TITLE     Suppression of the novel ER protein Maxer by mutant ataxin-1 in
            Bergman glia contributes to non-cell-autonomous toxicity
  JOURNAL   EMBO J 29 (14), 2446-2460 (2010)
   PUBMED   20531390
  REMARK    GeneRIF: Results identify a novel molecule named multiple
            alpha-helix protein located at ER (Maxer) downregulated by mutant
            ataxin-1 (Atx1) in Bergmann glia.
REFERENCE   7  (bases 1 to 5327)
  AUTHORS   Wu,J., Lei,G., Mei,M., Tang,Y. and Li,H.
  TITLE     A novel C53/LZAP-interacting protein regulates stability of
            C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and
            modulates NF-kappaB signaling
  JOURNAL   J Biol Chem 285 (20), 15126-15136 (2010)
   PUBMED   20228063
REFERENCE   8  (bases 1 to 5327)
  AUTHORS   Lee,J., Xu,Y., Chen,Y., Sprung,R., Kim,S.C., Xie,S. and Zhao,Y.
  TITLE     Mitochondrial phosphoproteome revealed by an improved IMAC method
            and MS/MS/MS
  JOURNAL   Mol Cell Proteomics 6 (4), 669-676 (2007)
   PUBMED   17208939
REFERENCE   9  (bases 1 to 5327)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 5327)
  AUTHORS   Eickhoff,H., Schuchhardt,J., Ivanov,I., Meier-Ewert,S., O'Brien,J.,
            Malik,A., Tandon,N., Wolski,E.W., Rohlfs,E., Nyarsik,L.,
            Reinhardt,R., Nietfeld,W. and Lehrach,H.
  TITLE     Tissue gene expression analysis using arrayed normalized cDNA
            libraries
  JOURNAL   Genome Res 10 (8), 1230-1240 (2000)
   PUBMED   10958641
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY097965.1, AK132697.1, AL365061.1, AL935267.13 and BM900014.1.
            
            On Sep 19, 2017 this sequence version replaced XM_006538203.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.4328.1,
                                           SRR9219379.19199.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               BY097965.1         2-103
            103-2392            AK132697.1         67-2356
            2393-2662           AL365061.1         131-400
            2663-3862           AK132697.1         3083-4282
            3863-5042           AL935267.13        31205-32384         c
            5043-5327           BM900014.1         1-285               c
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 10.59 cM"
     gene            1..5327
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="UFM1 specific ligase 1"
                     /db_xref="GeneID:67490"
                     /db_xref="MGI:MGI:1914740"
     exon            1..146
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2451
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="E3 UFM1-protein transferase 1; regulator of
                     C53/LZAP and DDRGK1; multiple alpha-helix protein located
                     at ER"
                     /codon_start=1
                     /product="E3 UFM1-protein ligase 1"
                     /protein_id="NP_001342441.1"
                     /db_xref="CCDS:CCDS18010.1"
                     /db_xref="GeneID:67490"
                     /db_xref="MGI:MGI:1914740"
                     /translation="MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQ
                     LEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIK
                     SEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQR
                     LGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLY
                     SVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLG
                     IPDAVNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLS
                     VEDAAMLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNP
                     VHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKK
                     KGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKP
                     LNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQT
                     ALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTK
                     LHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPA
                     LILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVS
                     QNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE"
     exon            147..292
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            293..321
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            322..419
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            420..534
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            535..665
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            666..724
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            725..871
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1047
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1227
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1348
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1471
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1589
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1753
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1884
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1965
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2051
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2232
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2495
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..5316
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3842..3847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="hexamer: AATAAA"
     polyA_site      3865
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="major polyA site"
     regulatory      3938..3943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="hexamer: ATTACA"
     polyA_site      3962
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
     regulatory      3995..4000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="hexamer: ACTAAA"
     polyA_site      4030
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
     regulatory      5295..5300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /note="hexamer: ATTAAA"
     polyA_site      5316
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
ORIGIN      
        1 ggggtgcctg cggcaggctg aaggctgacg tgtctgccgt ttccctgtgt cggggcgagc
       61 ggagcagtga tggcggacgc ctgggaggag atccggcggc tggcggctga cttccagcgg
      121 gcgcagtttg cagagtccac gcaaaggttg tcagagcgga actgcattga gattgtgaat
      181 aaattgatct ctcagaaaca gctggaagta gtccatacac ttgacggcaa ggaatatatc
      241 actccagccc aaatcagtaa agaaatgaga gacgagctac atgtccgagg tggtcgagta
      301 aacattgttg acttacaaca ggttattaat gtggacctga ctcatatcga gagtagagtc
      361 agtgatatta ttaaatcaga gaagcacgtg cagatggtac tgggacaact gattgatgag
      421 aactatctgg atcagttatc agaagaggtg aatgataaat tgcaagaaag tggtcaagta
      481 actgtctcag aactgtgtaa agcctatgac cttcctgggg attttctgac acaggcatta
      541 actcagcgac ttggccggat tatcaatggc catctggacc ttgataacag aggagtgatt
      601 ttcactgagg cctttgtagc tcgccataaa gcacgtatcc gcggactctt cagtgccatt
      661 acacggccta caccagtgaa ctctttggtt tcaaaatatg gatttcagga acagctcctt
      721 tactctgtgc tcgaggacct cgtcagcact ggccgcttac gaggcaccgt ggtcggcggg
      781 cgacaggata aagcagtctt tgtccctgac atctactcca ggacacagag tacttgggtg
      841 gactcctttt tcaggcagaa tggctatcta gaatttgacg ctttatccag acttggaatc
      901 ccagatgctg tgaactatat aaagaaaagg tataagaata cccaactctt gtttttgaag
      961 gcaacttgtg ttggccaagg actcgtagac caggtggaag catccgtaga ggaagccatc
     1021 agctcaggga cctgggttga catttcgcct ctgctaccca gttccttgtc agttgaagat
     1081 gctgctatgc tgctccagca ggtgatgaga ccattcggca agctcgcttc agctatagtc
     1141 tttagtgaca cggttgtggt gagtgaaaag ttcataactg actgcacagg actgttcagc
     1201 gaacggatgc accaaaaagc tgagaaggaa atgaaaaata atcctgtgca tttaatcact
     1261 gaagaagatc tgaaacaaat ttccatttta gaaagtgtta atacaagtaa aaaggataaa
     1321 aaagatgaac gcaggaagaa agcaacagag ggctctggaa gtgtaagagg aggaggaggg
     1381 ggcaatgcca gagaatataa aattaagaaa accaagaaga agggaagaaa agatgaggac
     1441 agtgatgatg agtcccagtc gtcgcatggt ggaaagaaga agccggacat cacattcatg
     1501 ttccaggatg aaattgaaga ttgcttaagg aaacacattc aggatgcccc tgaggagttt
     1561 atttcagagc ttgcagaata cttaataaag cctcttaata aaatgtatct tgaggtagta
     1621 cgttcagtct tcatgtcttc aacatctgct tctggaactg gcagaaaacg cacaatcaag
     1681 gacttacaag aagaggtttc aaacctatac aataatatca gattatttga aaagggaatg
     1741 aaatattttg cagatgacac acagactgct cttaccaagc acttgctgaa gacagtgtgt
     1801 accgatatca ctaacctcat gttcaacttc ctagcctcag atttcctcat ggcagtggag
     1861 gagcctgcag ccattacaag tgatataaga aaaaaaattt taagcaaatt gacagaagaa
     1921 accaaagtag ctctcacaaa actccataac tctctcaatg aaaaaagcat agaagacttc
     1981 ctgtcttgcc tggattctgc cacagaagcg tgtgacatta tggtgaaaaa gggagacaag
     2041 aaaagggaaa gacaaatcct cttccagcat cgacaggctc tgtgtgagca gctgaaggtc
     2101 acagaagacc ctgctctgat tttacacctc acagcagtcc tgttatttca gctctcaacc
     2161 cacagcatgc tccacgcacc tggaaggtgt gtcccacaga tcatcgcgtt tcttcacagc
     2221 aaaatcccag aggatcaaca tactctcttg gtaaaatatc aaggtttggt tgtgaaacag
     2281 ttagtcagtc aaaataagaa gactgggcag ggagaggatc cgtcaagcga tgaattagac
     2341 aaggaacaac atgatgtcac aaacgctact cggaaagagc ttcaggaact gtcattatcc
     2401 attaaggacc ttgttctcaa gtccaggaaa tcatctgtca cagaggagta atgcccctaa
     2461 ctgacttttg ttacatagta ttttccccaa agtagctaga aggaaggcct cctgcatgaa
     2521 catctccaga ggcctcagaa gtgatttaac aagtgagaac actgaagaag caagcattgc
     2581 tgtgttcgaa taaagcaagc cacagggtac cgtgagggaa agcgtgaggc acgccgcaga
     2641 acagtgcaaa ccatgtgtct gtctgagggg ctaaaggcac agagatgctt ctgtcattag
     2701 cccagtttag agttctaagg tttagttgtt tgatttccgt ggctgttttt acccttagtt
     2761 ctttgttgga aattgtatga caggtagcta ggctgttcta atgaacacag aatctgtggg
     2821 tgtttaaact gtgcagaaat tatattctaa aagtagacgt gtttaaaagt tgaatttttg
     2881 ttttctatga ttattaggta agacatacta aaacagttcc ttggaacaat aatttaaagc
     2941 aagttataag caacatccta ttgtcaaaat tttagttttt ctgaatgtaa taaaattaaa
     3001 acccagatgt ttaaggaatg ctattttata taatgtcaat ggaattttta tattttaaaa
     3061 tgtttaaata tataaataac actacctctt ttctgaaatg gcaaagtgtt aataatactg
     3121 catagaagtc cttaattatt aattaaaaat tttattaaag ttgatgatag agatttatag
     3181 aaaacattaa ggggctgtag aggtggctca gtgggtgctg ctgttgttgt tgttgctgtt
     3241 gttgttgttg ttgttgttgc tgttgttgtt gttgttgatg gtgatgattt gcagcatcac
     3301 atggatgctt tgtatctcca gtcccaggaa agccaatgct ttctggccac tccgggcact
     3361 aagcatacac aagatgtata ggcgcacatg caggcaaaac acccatacac ataaaagaat
     3421 ataaatgatt tcacatgggt aatttttatt gtatattttc atataaccaa aggaagaaaa
     3481 gtaataaatt ctatgactcc aaaactactt tattttcgta ttcagcttcc aaattagtac
     3541 ccaacctatt caacttttgt ttaataacat gtttcaaaaa atacaaaagt gatccatatt
     3601 tgcttcatac tctacaatta aagtaaacac aagagatgat cacataaact cagtttaatt
     3661 acaaaactaa agtaggaaaa tgtaaagctg tgttaatctt acttgcattt atttcatttg
     3721 tccaaacagt ttctattttt ccttcataaa aaaacatgtt tataaattat acagctgatt
     3781 ttgtctctat agatttaatt tctgatattt tttaatggac tgagttaaga acattattta
     3841 aaataaagtt gtctattcta ttacatttta tgttatacag tctatgtaat gcttatattt
     3901 taaattattc tgattgttaa tggagatgac atttcttatt acacaaaaat aacttccttg
     3961 tatgttctat tgcttgaatg tttgccatta tcttactaaa tcatttctca gccagatttc
     4021 agcaaatcta taatgttgtt aggtctgtta actgttgcca ttataggaga gatctgggaa
     4081 aacacgcccc ggtttccatc tttacactgt tataaaggaa acccactctg accacctaca
     4141 acagcggttc tcaacttgtg ggttgtgcct gacccctcgt tagttgtcaa aggacccttt
     4201 cgtgggggtt gcccagtgcc atggaaaaaa catatattta cattttgatt catgaaagta
     4261 gcaaacttac agttatgaag taacaataaa ataatttcat ggctgggagt caatacaact
     4321 tcatggctgg gggtcaatac aacatcaact atattaaagg gtcacagcat taggaaagtt
     4381 gagaaccact gccctagaag atagaatctc actgtgcttt gagcaaagac cataatgcta
     4441 tagaatagta ataaaaagtg tataacactc tgtgtagggc taccctggac atgttagaga
     4501 agctttttac ccagataatg aacaaggtca aagtttacca atgaacaatc attggttttg
     4561 tcgaaatgtc atcaaccacg ggcataaatg ttttattgtt gcctttctat caaaaactac
     4621 ttatgataaa ttggatgaaa ggagagtgga gaggaaactt tattttcttt aaattctatg
     4681 aagtctttac ctcagtagac ctgagagaga aggtaaaaat acacattgaa aactctggga
     4741 tgatattcca cacgcatttt gattcattac tattgacagg attgtttttt aacgaaaaaa
     4801 caatgtttaa ctattaaaaa aattaaaata ataagtagcc ttaaaaaggg ttagacatat
     4861 tgttctactt ttatgtacat ttaacaaatg agaaaagaga taggagagtc gtatacattc
     4921 actaagaacc ctgacttaca ggtgttcaga aaacagttgc acgacacaca aaaggaggag
     4981 gaacatttag ttaagtatga ttcatgttca cagtctagta cacaagagag aaatcctttg
     5041 tctgcctcag ccagccagcc aaaagtacaa aagttagttc atcttttatg cttcaatggg
     5101 aggaagtaaa attatttcct ttctgagcct ttaggccttt aacttaatta gacctcaaga
     5161 ttcgacaagc aactgaaggc ataatgaaag gttggcacaa aaaatgcata cctgtaacct
     5221 cctgtgggtt acaagatcaa agccagcctt agccacatgg tagatgtctc agaaaatgca
     5281 gttgttcatg tagtattaaa atgtaaatga aattaaaaaa aaaaaaa
//
