LOCUS       NP_001342441             793 aa            linear   ROD 09-FEB-2025
DEFINITION  E3 UFM1-protein ligase 1 [Mus musculus].
ACCESSION   NP_001342441 XP_006538266
VERSION     NP_001342441.1
DBSOURCE    REFSEQ: accession NM_001355512.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 793)
  AUTHORS   He,C., Xing,X., Chen,H.Y., Gao,M., Shi,J., Xiang,B., Xiao,X.,
            Sun,Y., Yu,H., Xu,G., Yao,Y., Xie,Z., Xing,Y., Budiarto,B.R.,
            Chen,S.Y., Gao,Y., Lee,Y.R. and Zhang,J.
  TITLE     UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance
            anti-tumor immunity
  JOURNAL   Mol Cell 84 (6), 1120-1138 (2024)
   PUBMED   38377992
  REMARK    GeneRIF: UFL1 ablation in T cells suppresses PD-1 UFMylation to
            enhance anti-tumor immunity.
REFERENCE   2  (residues 1 to 793)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 793)
  AUTHORS   Chen,F., Sheng,L., Zhou,T., Yan,L., Loveless,R., Li,H., Teng,Y. and
            Cai,Y.
  TITLE     Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological
            damage and carcinogenesis through activating mTOR signaling
  JOURNAL   J Exp Clin Cancer Res 42 (1), 110 (2023)
   PUBMED   37131258
  REMARK    GeneRIF: Loss of Ufl1/Ufbp1 in hepatocytes promotes liver
            pathological damage and carcinogenesis through activating mTOR
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 793)
  AUTHORS   Zhou,J., Ma,X., He,X., Chen,B., Yuan,J., Jin,Z., Li,L., Wang,Z.,
            Xiao,Q., Cai,Y., Zou,Y. and Cong,Y.S.
  TITLE     Dysregulation of PD-L1 by UFMylation imparts tumor immune evasion
            and identified as a potential therapeutic target
  JOURNAL   Proc Natl Acad Sci U S A 120 (11), e2215732120 (2023)
   PUBMED   36893266
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2023 May 23;120(21):e2306087120.
            doi: 10.1073/pnas.2306087120.. PMID: 37186868]
REFERENCE   5  (residues 1 to 793)
  AUTHORS   Wang,K., Xu,H.N., Wang,Y.W., Mao,J., Liu,D., Zhu,X.J., Cong,Y.S.
            and Wang,M.
  TITLE     Ufl1 deficiency causes skin pigmentation by up-regulation of
            Endothelin-1
  JOURNAL   Front Cell Dev Biol 10, 961675 (2022)
   PUBMED   36120581
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 793)
  AUTHORS   Shiwaku,H., Yoshimura,N., Tamura,T., Sone,M., Ogishima,S.,
            Watase,K., Tagawa,K. and Okazawa,H.
  TITLE     Suppression of the novel ER protein Maxer by mutant ataxin-1 in
            Bergman glia contributes to non-cell-autonomous toxicity
  JOURNAL   EMBO J 29 (14), 2446-2460 (2010)
   PUBMED   20531390
  REMARK    GeneRIF: Results identify a novel molecule named multiple
            alpha-helix protein located at ER (Maxer) downregulated by mutant
            ataxin-1 (Atx1) in Bergmann glia.
REFERENCE   7  (residues 1 to 793)
  AUTHORS   Wu,J., Lei,G., Mei,M., Tang,Y. and Li,H.
  TITLE     A novel C53/LZAP-interacting protein regulates stability of
            C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and
            modulates NF-kappaB signaling
  JOURNAL   J Biol Chem 285 (20), 15126-15136 (2010)
   PUBMED   20228063
REFERENCE   8  (residues 1 to 793)
  AUTHORS   Lee,J., Xu,Y., Chen,Y., Sprung,R., Kim,S.C., Xie,S. and Zhao,Y.
  TITLE     Mitochondrial phosphoproteome revealed by an improved IMAC method
            and MS/MS/MS
  JOURNAL   Mol Cell Proteomics 6 (4), 669-676 (2007)
   PUBMED   17208939
REFERENCE   9  (residues 1 to 793)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (residues 1 to 793)
  AUTHORS   Eickhoff,H., Schuchhardt,J., Ivanov,I., Meier-Ewert,S., O'Brien,J.,
            Malik,A., Tandon,N., Wolski,E.W., Rohlfs,E., Nyarsik,L.,
            Reinhardt,R., Nietfeld,W. and Lehrach,H.
  TITLE     Tissue gene expression analysis using arrayed normalized cDNA
            libraries
  JOURNAL   Genome Res 10 (8), 1230-1240 (2000)
   PUBMED   10958641
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY097965.1, AK132697.1, AL365061.1, AL935267.13 and BM900014.1.
            
            On Sep 19, 2017 this sequence version replaced XP_006538266.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.4328.1,
                                           SRR9219379.19199.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 10.59 cM"
     Protein         1..793
                     /product="E3 UFM1-protein ligase 1"
                     /note="E3 UFM1-protein transferase 1; regulator of
                     C53/LZAP and DDRGK1; multiple alpha-helix protein located
                     at ER"
                     /calculated_mol_wt=89389
     Region          2..212
                     /region_name="Required for E3 UFM1-protein ligase
                     activity. /evidence=ECO:0000250|UniProtKB:O94874"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          2..200
                     /region_name="Mediates interaction with DDRGK1.
                     /evidence=ECO:0000250|UniProtKB:O94874"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:O94874; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          7..284
                     /region_name="E3_UFM1_ligase"
                     /note="E3 UFM1-protein ligase 1; pfam09743"
                     /db_xref="CDD:462874"
     Region          121..250
                     /region_name="Involved in CDK5RAP3-binding.
                     /evidence=ECO:0000250|UniProtKB:O94874"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          200..400
                     /region_name="Mediates interaction with TRIP4.
                     /evidence=ECO:0000250|UniProtKB:O94874"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          410..473
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            433
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            458
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17208939; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            462
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O94874; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          490..683
                     /region_name="Mediates interaction with CDK5RAP3.
                     /evidence=ECO:0000250|UniProtKB:B2GV24"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            535
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:O94874; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Region          742..765
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            752
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     Site            753
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355; propagated from
                     UniProtKB/Swiss-Prot (Q8CCJ3.2)"
     CDS             1..793
                     /gene="Ufl1"
                     /gene_synonym="1810074P20Rik; Kiaa0776; Maxer; mKIAA0776;
                     Rcad"
                     /coded_by="NM_001355512.1:70..2451"
                     /db_xref="CCDS:CCDS18010.1"
                     /db_xref="GeneID:67490"
                     /db_xref="MGI:MGI:1914740"
ORIGIN      
        1 madaweeirr laadfqraqf aestqrlser ncieivnkli sqkqlevvht ldgkeyitpa
       61 qiskemrdel hvrggrvniv dlqqvinvdl thiesrvsdi iksekhvqmv lgqlidenyl
      121 dqlseevndk lqesgqvtvs elckaydlpg dfltqaltqr lgriinghld ldnrgvifte
      181 afvarhkari rglfsaitrp tpvnslvsky gfqeqllysv ledlvstgrl rgtvvggrqd
      241 kavfvpdiys rtqstwvdsf frqngylefd alsrlgipda vnyikkrykn tqllflkatc
      301 vgqglvdqve asveeaissg twvdispllp sslsvedaam llqqvmrpfg klasaivfsd
      361 tvvvsekfit dctglfserm hqkaekemkn npvhliteed lkqisilesv ntskkdkkde
      421 rrkkategsg svrgggggna reykikktkk kgrkdedsdd esqsshggkk kpditfmfqd
      481 eiedclrkhi qdapeefise laeylikpln kmylevvrsv fmsstsasgt grkrtikdlq
      541 eevsnlynni rlfekgmkyf addtqtaltk hllktvctdi tnlmfnflas dflmaveepa
      601 aitsdirkki lsklteetkv altklhnsln eksiedflsc ldsateacdi mvkkgdkkre
      661 rqilfqhrqa lceqlkvted palilhltav llfqlsthsm lhapgrcvpq iiaflhskip
      721 edqhtllvky qglvvkqlvs qnkktgqged pssdeldkeq hdvtnatrke lqelslsikd
      781 lvlksrkssv tee
//
