LOCUS       NM_001113558             884 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus upper zone of growth plate and cartilage matrix
            associated (Ucma), transcript variant 2, mRNA.
ACCESSION   NM_001113558
VERSION     NM_001113558.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Chen,Y., Wu,J., Zhang,S., Gao,W., Liao,Z., Zhou,T., Li,Y., Su,D.,
            Liu,H., Yang,X., Su,P. and Xu,C.
  TITLE     Hnrnpk maintains chondrocytes survival and function during growth
            plate development via regulating Hif1alpha-glycolysis axis
  JOURNAL   Cell Death Dis 13 (9), 803 (2022)
   PUBMED   36127325
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 884)
  AUTHORS   Lee,S.H., Lee,Y.J., Park,S.I. and Kim,J.E.
  TITLE     Unique cartilage matrix-associated protein inhibits the migratory
            and invasive potential of triple-negative breast cancer
  JOURNAL   Biochem Biophys Res Commun 530 (4), 680-685 (2020)
   PUBMED   32768190
  REMARK    GeneRIF: Unique cartilage matrix-associated protein inhibits the
            migratory and invasive potential of triple-negative breast cancer.
REFERENCE   4  (bases 1 to 884)
  AUTHORS   Lee,Y.J., Park,S.Y., Park,E.K. and Kim,J.E.
  TITLE     Unique cartilage matrix-associated protein regulates fibrillin-2
            expression and directly interacts with fibrillin-2 protein
            independent of calcium binding
  JOURNAL   Biochem Biophys Res Commun 511 (2), 221-227 (2019)
   PUBMED   30791984
  REMARK    GeneRIF: Downstream target genes of Ucma were found in osteoblasts
            by microarray analysis. 45 genes including Fbn2 were dependent on
            Ucma overexpression or knockdown. Fbn2 expression was proportional
            to Ucma expression in osteoblasts.
REFERENCE   5  (bases 1 to 884)
  AUTHORS   Le Jeune,M., Tomavo,N., Tian,T.V., Flourens,A., Marchand,N.,
            Camuzeaux,B., Mallein-Gerin,F. and Duterque-Coquillaud,M.
  TITLE     Identification of four alternatively spliced transcripts of the
            Ucma/GRP gene, encoding a new Gla-containing protein
  JOURNAL   Exp Cell Res 316 (2), 203-215 (2010)
   PUBMED   19819238
  REMARK    GeneRIF: Four alternative splicing variants derived from the mouse
            Ucma/GRP gene in chondrocytes were identified and their expression
            and subcellular localization during chondrogenesis was determined.
REFERENCE   6  (bases 1 to 884)
  AUTHORS   Wuelling,M., Kaiser,F.J., Buelens,L.A., Braunholz,D.,
            Shivdasani,R.A., Depping,R. and Vortkamp,A.
  TITLE     Trps1, a regulator of chondrocyte proliferation and
            differentiation, interacts with the activator form of Gli3
  JOURNAL   Dev Biol 328 (1), 40-53 (2009)
   PUBMED   19389374
REFERENCE   7  (bases 1 to 884)
  AUTHORS   Retting,K.N., Song,B., Yoon,B.S. and Lyons,K.M.
  TITLE     BMP canonical Smad signaling through Smad1 and Smad5 is required
            for endochondral bone formation
  JOURNAL   Development 136 (7), 1093-1104 (2009)
   PUBMED   19224984
REFERENCE   8  (bases 1 to 884)
  AUTHORS   Surmann-Schmitt,C., Dietz,U., Kireva,T., Adam,N., Park,J.,
            Tagariello,A., Onnerfjord,P., Heinegard,D.,
            Schlotzer-Schrehardt,U., Deutzmann,R., von der Mark,K. and Stock,M.
  TITLE     Ucma, a novel secreted cartilage-specific protein with implications
            in osteogenesis
  JOURNAL   J Biol Chem 283 (11), 7082-7093 (2008)
   PUBMED   18156182
  REMARK    GeneRIF: Ucma may be involved in the negative control of osteogenic
            differentiation of osteochondrogenic precursor cells
REFERENCE   9  (bases 1 to 884)
  AUTHORS   Tagariello,A., Luther,J., Streiter,M., Didt-Koziel,L., Wuelling,M.,
            Surmann-Schmitt,C., Stock,M., Adam,N., Vortkamp,A. and
            Winterpacht,A.
  TITLE     Ucma--A novel secreted factor represents a highly specific marker
            for distal chondrocytes
  JOURNAL   Matrix Biol 27 (1), 3-11 (2008)
   PUBMED   17707622
  REMARK    GeneRIF: The present data strongly suggest an important function of
            Ucma in the early phase of chondrocyte differentiation.
REFERENCE   10 (bases 1 to 884)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from EU368184.1 and BY590633.1.
            
            On Sep 30, 2009 this sequence version replaced NM_001113558.1.
            
            Summary: This gene encodes chondrocyte-specific, highly charged
            proteins that are abundantly expressed during the early stages of
            chondrogenesis. The encoded protein undergoes proteolytic
            processing to generate a mature protein that is secreted into the
            extracellular matrix. The glutamic acid residues in the encoded
            protein undergo gamma carboxylation in a vitamin K-dependent
            manner. Despite the implied role in calcification and ossification,
            mice lacking the encoded protein do not display significant defects
            in the skeletal development. Alternative splicing results in
            multiple transcript variants encoding different isoforms that may
            undergo a similar proteolytic processing to generate mature
            proteins. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (2, also known as Ucma/GRP-F1)
            contains an alternate in-frame exon in the 5' coding region
            compared to variant 1. The encoded isoform (b) is longer than
            isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU368184.1, CB588171.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-881               EU368184.1         1-881
            882-884             BY590633.1         458-460
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 3.11 cM"
     gene            1..884
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /note="upper zone of growth plate and cartilage matrix
                     associated"
                     /db_xref="GeneID:68527"
                     /db_xref="MGI:MGI:1915777"
     exon            1..188
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..547
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /note="isoform b preproprotein is encoded by transcript
                     variant 2; unique cartilage matrix-associated protein;
                     Gla-rich protein; upper zone of growth plate and cartilage
                     matrix associated protein"
                     /codon_start=1
                     /product="unique cartilage matrix-associated protein
                     isoform b preproprotein"
                     /protein_id="NP_001107030.1"
                     /db_xref="CCDS:CCDS50489.1"
                     /db_xref="GeneID:68527"
                     /db_xref="MGI:MGI:1915777"
                     /translation="MSWRRVILLSSLLALVLLCMLQEGTSASVGSRQAAAEGVQEGVK
                     QKIFMQESDASNFLKRRGKRSPKSRDEVNAENRQRLRDDELRREYYEEQRNEFENFVE
                     EQRDEQEERTREAVEQWRQWHYDGLYPSYLYNRQNI"
     exon            189..254
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /inference="alignment:Splign:2.1.0"
     exon            255..350
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /inference="alignment:Splign:2.1.0"
     exon            351..449
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /inference="alignment:Splign:2.1.0"
     exon            450..884
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      865..870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /note="hexamer: AATAAA"
     polyA_site      883
                     /gene="Ucma"
                     /gene_synonym="1110017I16Rik; Grp"
                     /note="major polyA site"
ORIGIN      
        1 tctctctctc ttgcctgtct gcctgactca gaagtgagga cttactgggc agctgcttca
       61 gagtactggg gacttccaag atacctcacc ttattatctg tcctgtggtt tctgggggtg
      121 ctctgtaaag atgtcctgga gacgggtcat tctcctgtca tctctcttgg ccctggtgct
      181 cctgtgtatg ctacaggagg ggaccagcgc ttctgtgggg agcaggcagg cagctgcaga
      241 gggggtgcag gaaggtgtga aacagaagat tttcatgcaa gaatctgatg cctccaattt
      301 cctcaagagg cgtggcaagc ggtctcctaa gtcccgagat gaagttaatg cggaaaacag
      361 acagaggctg cgggatgatg agctgcggag ggagtattac gaggagcaaa ggaacgagtt
      421 tgagaacttc gtggaggaac agagagatga gcaggaagag aggacccggg aggctgtgga
      481 gcagtggcgc cagtggcatt atgatggcct gtatccttcc tacctctaca accgccaaaa
      541 catctgacct cattaaagca accggggaag agaagcatgt gacaggcctg ccacctctcc
      601 acagacggac ccacaaggtg aagctccctc accgccctgg tcaacagctc caggaaagac
      661 accctctgtt ctgatgcctt ctgatgcgtg gcttttgctt ctttatggat gtttgttttg
      721 ttttgttttt gagtgagcaa ccatctgtgg ttcttcccag cacctgcccc cttgcttagc
      781 caccagaaac agaatcagag cacccatctg atagacactt tcaggatcga agcataaagg
      841 ttttaagctt gcttatggcc tattaataaa agtgccgaat caac
//
