LOCUS       NM_177783               8086 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ubiquitin protein ligase E3 component n-recognin 3
            (Ubr3), transcript variant 2, mRNA.
ACCESSION   NM_177783 XM_992968
VERSION     NM_177783.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Reichmann,J., Dobie,K., Lister,L.M., Crichton,J.H., Best,D.,
            MacLennan,M., Read,D., Raymond,E.S., Hung,C.C., Boyle,S.,
            Shirahige,K., Cooke,H.J., Herbert,M. and Adams,I.R.
  TITLE     Tex19.1 inhibits the N-end rule pathway and maintains acetylated
            SMC3 cohesin and sister chromatid cohesion in oocytes
  JOURNAL   J Cell Biol 219 (5) (2020)
   PUBMED   32232464
  REMARK    GeneRIF: Tex19.1 inhibits the N-end rule pathway and maintains
            acetylated SMC3 cohesin and sister chromatid cohesion in oocytes.
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Li,T., Fan,J., Blanco-Sanchez,B., Giagtzoglou,N., Lin,G.,
            Yamamoto,S., Jaiswal,M., Chen,K., Zhang,J., Wei,W., Lewis,M.T.,
            Groves,A.K., Westerfield,M., Jia,J. and Bellen,H.J.
  TITLE     Ubr3, a Novel Modulator of Hh Signaling Affects the Degradation of
            Costal-2 and Kif7 through Poly-ubiquitination
  JOURNAL   PLoS Genet 12 (5), e1006054 (2016)
   PUBMED   27195754
  REMARK    GeneRIF: Mouse UBR3 poly-ubiquitinates Kif7, the mammalian
            homologue of Cos2. Finally, loss of UBR3 up-regulates Kif7 protein
            levels and decreases Hh signaling in cultured cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8086)
  AUTHORS   Meisenberg,C., Tait,P.S., Dianova,I.I., Wright,K., Edelmann,M.J.,
            Ternette,N., Tasaki,T., Kessler,B.M., Parsons,J.L., Kwon,Y.T. and
            Dianov,G.L.
  TITLE     Ubiquitin ligase UBR3 regulates cellular levels of the essential
            DNA repair protein APE1 and is required for genome stability
  JOURNAL   Nucleic Acids Res 40 (2), 701-711 (2012)
   PUBMED   21933813
  REMARK    GeneRIF: Knockout of the Ubr3 gene in mouse embryonic fibroblasts
            leads to an up-regulation of the cellular levels of APE1 protein
            and subsequent genomic instability.
REFERENCE   4  (bases 1 to 8086)
  AUTHORS   Tasaki,T., Sohr,R., Xia,Z., Hellweg,R., Hortnagl,H., Varshavsky,A.
            and Kwon,Y.T.
  TITLE     Biochemical and genetic studies of UBR3, a ubiquitin ligase with a
            function in olfactory and other sensory systems
  JOURNAL   J Biol Chem 282 (25), 18510-18520 (2007)
   PUBMED   17462990
  REMARK    GeneRIF: the UBR box of UBR3 may recognize small compounds that
            modulate the targeting, by this E3, of its currently unknown
            substrates
REFERENCE   5  (bases 1 to 8086)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   6  (bases 1 to 8086)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 8086)
  AUTHORS   Kwon,Y.T., Balogh,S.A., Davydov,I.V., Kashina,A.S., Yoon,J.K.,
            Xie,Y., Gaur,A., Hyde,L., Denenberg,V.H. and Varshavsky,A.
  TITLE     Altered activity, social behavior, and spatial memory in mice
            lacking the NTAN1p amidase and the asparagine branch of the N-end
            rule pathway
  JOURNAL   Mol Cell Biol 20 (11), 4135-4148 (2000)
   PUBMED   10805755
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929249.15, AK037622.1, BQ571759.1, AK050359.1 and CO042454.1.
            
            On Dec 16, 2014 this sequence version replaced NM_177783.5.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 3. This
            results in isoform (2) which is shorter than isoform 3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ924536.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AL929249.15        31744-31834
            92-734              AK037622.1         36-678
            735-737             AL929249.15        51575-51577
            738-1514            AK037622.1         679-1455
            1515-1694           AL929249.15        78798-78977
            1695-1828           AL929249.15        79512-79645
            1829-1915           AL929249.15        82922-83008
            1916-1981           AL929249.15        85841-85906
            1982-2071           AL929249.15        85998-86087
            2072-2200           AL929249.15        87239-87367
            2201-2253           AL929249.15        87986-88038
            2254-2387           AL929249.15        88141-88274
            2388-2473           AL929249.15        88774-88859
            2474-2615           AL929249.15        90463-90604
            2616-2712           AL929249.15        94065-94161
            2713-2854           AL929249.15        99872-100013
            2855-2959           AL929249.15        104635-104739
            2960-3133           AL929249.15        105558-105731
            3134-3421           AL929249.15        107647-107934
            3422-4048           BQ571759.1         22-648
            4049-4078           AL929249.15        123365-123394
            4079-4279           AL929249.15        125923-126123
            4280-4295           AL929249.15        128174-128189
            4296-5590           AK050359.1         1-1295
            5591-5601           AL929249.15        155739-155749
            5602-8068           AL929249.15        156041-158507
            8069-8086           CO042454.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 40.95 cM"
     gene            1..8086
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="ubiquitin protein ligase E3 component n-recognin 3"
                     /db_xref="GeneID:68795"
                     /db_xref="MGI:MGI:1861100"
     exon            1..594
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..5719
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase UBR3; N-recognin-3;
                     ubiquitin-protein ligase E3-alpha-3; ubiquitin-protein
                     ligase E3-alpha-III; zinc finger protein 650; RING-type E3
                     ubiquitin transferase UBR3"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase UBR3 isoform 2"
                     /protein_id="NP_808451.2"
                     /db_xref="CCDS:CCDS16104.2"
                     /db_xref="GeneID:68795"
                     /db_xref="MGI:MGI:1861100"
                     /translation="MAAAAAAAAVGDPQPPQPEAPAQGLALDKAATAAHLKAALSRPD
                     NRAGAEELQALLERVLNAERPLAGAAGGEEAAGGGGGGPGEAEEDALEWCKCLLAGGG
                     GYEEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFN
                     MFRSQAGGACDCGDSNVMRESGFCRRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLI
                     QYLREGYNEPAADAPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNY
                     KDLTAGLGENACAKKSHEKYLIALKSSGLTYPEDKLVYGVQEPAAGTSTLAAQGFAGA
                     TGTLGQIDSSDEEDQDGSQGLGKRKRVKLSSGTKDQSIMDVLKHKSFLEELLFWTIKY
                     EFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQL
                     FSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNN
                     TYWPLVSDFINILSHQSVAQRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQ
                     TYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPA
                     PNQVTFHLPLHRYYAMFLSKAVKCQELDLDSLLPDQEMLMKLMIHPLQIQASLAEIHS
                     NMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVD
                     LLTMASQHHNMVLDVEHERSMLEGALTFLVILLSLRLHLGMSDDDILRAEMVAQLCMN
                     DRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFRAPIFEPGGSMQQGMYTPKA
                     EVWDQEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTSLHPSYK
                     GLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSADDDSEEEVS
                     MGGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPELKRDPLASTSSDALDSLQNS
                     GTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKIPSKWSAPGSSPQLTTAILEIKES
                     ILSLLIKLHHKLSGKQNSYYPPWLDDIEVLIQPEIPKYNHGDGITAVERILLKAAVQS
                     RMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSF
                     METAMDVDSPENDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSV
                     LGQCRDNAEPKKLPIAEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGG
                     VYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSN
                     VENNLWQRPCNKSTQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRD
                     YSKTPGSPDNEFLFMYSVARTNLELELIHRGGSLCSGGPSTAGKRSCLNQLFHVLALH
                     MRLYTIDSEYNPWKKLTQLVEDMNSQVGNEDQQPEVPILYHDVTSLLLIQILMMPQPL
                     RKEHFTCIVKVLFTLLYTQALAALSVKGTEEDRSAWKHAGALRKDTCDAEKCYEVLLS
                     FVISELSKGKLYYEEGAQECAMVSPIAWSPESMERYIQDFCLPFLRVSSLLQHHLFGE
                     DLPSCQEEEEFSVLASCLGLLPTFYQTDHPFISASCLDWPVAAFDIITQWCFEITSFT
                     ERHAEQGKALLIQESRWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCG
                     TFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDA
                     HGEEDRDLRRGKPLYICEERYRVLEQQWVSHTFDHINKRWGPHYNGL"
     exon            595..734
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            735..893
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1037
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1087
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1154
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1285
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1514
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1694
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1828
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1915
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..1981
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2071
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2200
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2253
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2387
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2473
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2615
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2712
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2854
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..2959
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            2960..3133
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3594
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            3595..3683
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            3684..3833
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3967
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            3968..4078
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4279
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4280..4416
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4417..4502
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4503..4608
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4609..4712
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4713..4837
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4838..4945
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            4946..5071
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            5072..5251
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            5252..5461
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            5462..5601
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     exon            5602..8069
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6203..6208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="hexamer: AGTAAA"
     polyA_site      6225
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="major polyA site"
     regulatory      6580..6585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="hexamer: AATAAA"
     polyA_site      6606
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
     regulatory      8031..8036
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="hexamer: AATAAA"
     polyA_site      8069
                     /gene="Ubr3"
                     /gene_synonym="1110059H15Rik; 4833421P10Rik;
                     A130030D10Rik; Zfp650; Znf650"
                     /note="major polyA site"
ORIGIN      
        1 tcccgggagg agccgggggc ctggactctg caaattctga gctctcatca tggcggcggc
       61 ggcggccgcg gcggccgtcg gggacccgca gccgccccag ccggaggcgc cggcgcaggg
      121 gctggcttta gacaaagcgg ccaccgccgc tcatctgaag gccgctctca gccggccgga
      181 taaccgtgcg ggcgccgagg agctgcaggc gctgctggag agggtgctga acgccgagcg
      241 gccgctggcc ggggctgcgg gcggcgagga ggcggcgggc ggcggcggcg gcggcccggg
      301 cgaggcggag gaggatgccc tggagtggtg taagtgcctt ctggccggcg gcggcggcta
      361 cgaggagttc tgcgcggccg tgcgggccta cgaccccgcg gcgctctgcg gcctggtctg
      421 gacggccaac ttcgtggcct accgctgccg gacgtgcggc atctccccct gcatgtcgct
      481 gtgcgcggag tgcttccacc agggcgacca cactggacac gacttcaaca tgttccgcag
      541 ccaggccggg ggcgcctgcg actgcgggga cagcaacgta atgcgggaga gcgggttttg
      601 tagaagacat cagattaaat caagttcaaa tattccctgt gtccccaaag acttactgat
      661 gatgtctgaa tttgttcttc caagattcat tttttgtctt attcagtact taagagaagg
      721 ctataatgaa ccagcagctg atgcaccatc agaaaaagat ctgaacaaag tccttcagct
      781 tttggagcct cagatttcct ttttagaaga cctgactaaa atgggaggag ccatgaggtc
      841 tgttcttact caggtgttaa ccaaccagca gaactacaaa gacctgactg cgggtcttgg
      901 agaaaatgct tgtgcaaaga aaagtcacga gaagtacctt atagctttga agagctccgg
      961 actgacatat cccgaggata agctggtgta tggggtccag gaaccagctg ctggtactag
     1021 cactctggca gctcaaggtt ttgcaggtgc tactggaaca ctggggcaga tagactcttc
     1081 agatgaggag gaccaggatg ggagtcaagg cttgggcaag agaaagaggg tgaagctaag
     1141 cagtggcacc aaagatcaat ccataatgga tgttttgaaa cataaaagtt ttttagaaga
     1201 actattattt tggacaataa agtatgagtt cccacagaag atggtaactt tcttgctcaa
     1261 tatgctccca gatcaagagt ataaggttgc ttttacaaaa acatttgttc agcattatgc
     1321 ttttattatg aaaacactga agaaaagtca cgaatcagat acaatgtcca acagaattgt
     1381 gcatattagt gttcagttgt tcagcaatga ggagctagcc agacaggtga cagaagagtg
     1441 tcagctcctg gacatcatgg tcactgtgtt gttgtacatg atggagagct gcctcatcaa
     1501 gagtgagctg caagatgaag agaacagttt acatgtagtg gtgaactgtg gagaagcctt
     1561 actgaagaat aacacctact ggccccttgt cagtgatttt attaatattc tttctcatca
     1621 aagtgtggct cagaggtttc tggaggatca cggcctgtta gttacatgga tgaactttgt
     1681 gtctttcttt caaggtatga acttgaacaa gcgagagctc aatgagcacg tggaatttga
     1741 gtctcagacc tactatgctg cctttgctgc tgagctggaa gcctgtgcac aacccatgtg
     1801 ggggctttta tcacattgta aagttaggga aactcaagag tataccagga atgttgtaag
     1861 gtactgcctt gaagctcttc aagattggtt tgatgctatt aactttgtag acgagccagc
     1921 tcctaaccaa gtcactttcc acctaccact gcaccgctac tatgctatgt ttttgagtaa
     1981 ggctgtgaaa tgtcaagaac tagatttgga ttcactttta ccggatcagg aaatgttaat
     2041 gaaactaatg attcacccac tccaaattca agctagtctt gctgaaattc acagtaacat
     2101 gtgggtaaga aatggtctgc aaatcaaagg acaagccatg acctatgtcc agtctcattt
     2161 ctgtaactcc atgattgacc cagacattta cttactacag gtctgtgctt ctagacttga
     2221 cccagactac tttatatcat ctgtctttga aagatttaag gtggtggact tactgaccat
     2281 ggcttcacag caccacaaca tggtgctcga tgtggagcac gagcggtcga tgctagaggg
     2341 tgctctcacg ttcctcgtga ttcttttgag tcttcgttta cacctaggga tgtctgacga
     2401 tgacattctc agggcagaga tggtggccca gctgtgtatg aacgacagga cacatagctc
     2461 tttgcttgac ctcatcccag aaaatcccaa ccccaaaagt ggcattattc ctggaagtta
     2521 tagctttgaa tcagttttat ctgctgtagc ggactttagg gctcccattt ttgaacctgg
     2581 aggctctatg caacaaggca tgtacactcc caaagctgaa gtctgggatc aggagtttga
     2641 tcctgtcatg gtcattcttc gaaccgttta ccgccgagac gtgcagtctg caatggacag
     2701 atacacagca tttttaaaac agagtggaaa attccctggc aatccctggc ctccgtataa
     2761 gaaaagaaca tcactccatc ccagctataa aggtcttatg agacttctgc actgtaaaac
     2821 tttacacatc gtgctcttca ctctgcttta caagattctg atggatcatc aaaatctgtc
     2881 tgagcatgtg ctgtgcatgg tcttgtacct gattgagtta ggcctggaga actctgctga
     2941 cgatgactcc gaggaagagg tatcaatggg tggaccagag cgttgtcatg acagttggtt
     3001 ccctggcagt aacttggtat caaacatgag gcactttata aattatgtta gagtgcgagt
     3061 cccagagacg gctcctgagt tgaaaaggga tcccctcgcc agcacgagct ctgacgcttt
     3121 ggactcttta caaaattctg gtacagctca agttttcagc ttagtagcag agcgtagaaa
     3181 gaaatttcag gagatcatca atcgcagcaa cagtgaagca aatcaggtgg ttcggcccaa
     3241 gattccaagt aaatggtcag ctcctggttc ctctccccag ttaaccacag ccattttgga
     3301 aattaaggaa agcatattgt ctttgctaat aaaacttcac cacaaactct caggaaaaca
     3361 aaactcttac tatcctccat ggcttgatga catagaagtt ttaatacaac cagaaattcc
     3421 caaatacaat catggagatg gtataactgc tgtagaaagg attttgctaa aagctgcagt
     3481 gcaaagcaga atgaacaagc gcatcattga ggagatatgc agaaaagtga cccctcctgt
     3541 gccacccaaa aaaatcaccg cagcggagaa gaaaactctg gacaaggagg aaaggcgaca
     3601 aaaggctaga gaaaggcagc agaaattgct cgcagagttt gcctcacgac agaaaagctt
     3661 tatggaaact gcaatggatg ttgattctcc tgagaacgat attcctatgg aaattaccac
     3721 agcagaacca caagtttctg aggccgtgta tgactgtgtt atttgtggac agagtggtcc
     3781 ttcttctgaa gacagaccta caggactggt tgtactgtta caagcatcat cagttttagg
     3841 ccagtgccgg gacaatgctg agccaaaaaa gttgccgatc gctgaagagg agcagattta
     3901 cccttgggac acatgtgcag cagttcatga tgtgaggctt tcattactgc agcgttattt
     3961 taaagatagt tcttgccttt tggcagtatc aattggctgg gaaggaggtg tttatgtaca
     4021 aacttgtggc cacacgttgc acatagattg tcataaatcc tatatggagt cgttacggaa
     4081 tgaccaggtt cttcagggct tctcagtgga caaaggagaa ttcacgtgtc ccctctgtag
     4141 acagtttgcc aacagtgttc ttccatgtta tcctggaagc aatgtggaaa ataacctttg
     4201 gcaacgccct tgtaacaaaa gcacgcaaga tctcataaag gaagtggaag agctgcaggg
     4261 acggccggga gctttcccat cagaaaccaa cttaagtaaa gaaatggaat ctgtaatgaa
     4321 agatataaaa aataccactc agaagaaata cagagactat agcaagaccc caggctcacc
     4381 agacaacgag tttctcttta tgtactctgt tgctagaacc aatctggagc tggaactgat
     4441 tcatagagga ggcagtctct gctccggtgg tccaagcaca gcggggaaaa gatcttgtct
     4501 gaatcagctg ttccatgtat tagccttgca catgcggctt tataccattg attctgaata
     4561 taatccatgg aaaaaactca cccagttagt ggaagatatg aattcacagg tggggaatga
     4621 agaccagcag ccagaagttc caattcttta tcatgatgtg acatcccttc tgctcatcca
     4681 gatcttaatg atgccacaac ccttacgtaa agagcacttc acctgcattg tgaaagtact
     4741 cttcaccctg ctgtacacac aggccctggc ggcgctctca gttaagggca ctgaagagga
     4801 ccggtcagcc tggaaacacg ccggggctct tagaaaggat acatgtgatg cagaaaagtg
     4861 ttacgaagtg ctgttgagct ttgttataag tgagctgtct aaagggaagt tatattatga
     4921 agaaggcgct caggagtgtg cgatggtcag tcctattgct tggtctcctg agtccatgga
     4981 aagatacata caggatttct gcttgccttt cctcagggtc agcagccttc ttcagcacca
     5041 cctctttggg gaagacttac ccagttgcca ggaagaagaa gaattttcag tccttgccag
     5101 ctgcctggga cttctgccaa ctttttacca aacagatcac ccgttcatca gtgcctcgtg
     5161 tctggactgg ccagttgcag cgtttgacat tataactcag tggtgttttg agataacatc
     5221 atttacggag agacacgcag agcaaggaaa ggccttgctt atccaagaat caagatggaa
     5281 gttaccacac ctgttacagt tgcctgagaa ttacaacact atttttcagt actatcatag
     5341 aaaaacctgc agtgtctgca ccaaggttcc taaagaccca gctgtttgcc tcgtctgcgg
     5401 cactttcgta tgcctgaaag gcctgtgctg caagcagcaa agttactgtg aatgtgtgct
     5461 gcattctcag aattgtggtg caggaacagg aattttcctt ttaatcaacg cgtcagtgat
     5521 catcatcatc cgaggccacc gcttttgcct ctggggttcc gtctacttgg atgctcatgg
     5581 agaagaggac cgggacctca ggcgaggcaa gcctctctac atctgtgagg agcggtacag
     5641 agttctcgag cagcagtggg tttcccacac gtttgaccac atcaataaaa gatggggtcc
     5701 gcactacaat gggctgtgac acacacctca gcattgcata tattatcatt ttcactgaga
     5761 ttgattttca tcaacaataa gctttaattt aatttggggg gattgatgtc tttgctgagg
     5821 gataaaaaga aaacatacat tatgcagcct tcccaaaatt aggtgcttgg taaccacagt
     5881 aatggtataa tattttttct caagtatccg gacaacaaat aatttaaatc attctttagt
     5941 ggtcactttt taagtgcaca ataataaaag cacaactgtc cacagaaatc attgagaaat
     6001 gattgtccct ataacgccct gttggttgtt ccccgacaga gaggatgtgg tttatttgag
     6061 gtttccctgc tccttttgtg ttctcatttg catctgctaa acataatgca tcttttcctt
     6121 tgtcaccctt gtgttgattt gagattttcg ctgtatgtaa ttagaaacaa atgtaaaaca
     6181 tgatttatgt gaaatattgt atagtaaaaa attggtctaa tagtagaact ttacattttt
     6241 ttcttattgt gaggaatctg ttaaagttta aggctttgct gaaaacttaa ttcattctca
     6301 ggaatttcat aaatgctttc cgcgggtaaa tagttgaact agctgtaaga tacagtgcag
     6361 tgcagtgcag cgcacagagg gagggcaagg cgtgtgtgtg gttggggagg tccttagaaa
     6421 taagtgtgtg tgggctgagg gtggagctca gtggtagaaa gcttacctgg ctaacattta
     6481 cagaacccca gtttcatctt aagcaccgca catacacaaa atcaatttgc catttcaact
     6541 aaatgaaaat aagagttgtt agtgacgagc atatgttaca ataaactcaa aagggttaag
     6601 aacatataca tggcagagtt ttgattctgt gcatgcccac ctttccttac caaccaagcc
     6661 tttatttatg tgatcagatt gaaatgctca tctgcccaac acatgggcgt taagttctgg
     6721 gactctcctc agtgactgaa gaagctcctg tctgctgggc tcctgcagtg tcactgatgt
     6781 ttctgtgtgc ttcctacagc agaaaaggcc cagaggtact gaccctggga gtgagccgtg
     6841 cttctgcttt cagacattgc agtgaggatc agcttattgg tgggtgggtg gggggttaat
     6901 tcagaatggg aatgagtgag caaaaatcca cttagtaaga gggaaagaag atgacagaaa
     6961 aacatatttt attggtacca agactttttt ttccaggtga ttgaataatt tgatgattat
     7021 ttcatatata gtactatcaa gcaattcctg gtactttgga tttccatggc ttgctatata
     7081 aaattacatt tttttacttg taaaactaaa caacaaaaca ttgaatgaga aaatatcaaa
     7141 gtaacatcaa acccatgttc caaaattttt tgaattgatg atggcgttac agactatgaa
     7201 attaacataa aacagtggct ttgcattaaa atacggggaa cagagtagta cacacacaga
     7261 tgtgttcaga atgccagaag attgcctgga gcattttaag aaaatatgcc ggaatagctt
     7321 gctattttta tatacatttc caaaatggtg agataacttt gttaagagta gaatgacttc
     7381 ttcaggtaaa tccctgccct tctacacctg ctctctaaag tgctgcgcta caagctcagc
     7441 ctgctacctt ttttgcttcg gtcattataa atagcagacc atttgtaagt gtggttgaag
     7501 agcaaaatgc taattacatc tctagtaaat actgttacag gattcatgaa cttgagtaat
     7561 tttacagttt ggaactctga tgctatatat acatggtata atgtattcag actttgatca
     7621 ctacatattt aaatggaatg tttatggttg tgcatatgga tgtgaagtga aaatattagc
     7681 cttaacttta aaatttggat atttgatagt gtttattttg atattggtga attagtcacc
     7741 agtaaatttt aaaaaaagca cttggttgaa attgtacttg aataaataca tgcatgctgc
     7801 taaattagaa cctccattcc cctaactttt ataagtccca tttataaacc cacttttaaa
     7861 ataacaggtc caagttagag tgatgtgtgt atattattca aaaacatgta ctggtgtgat
     7921 cttgtatgaa tgatcattta aatacagtac attactgtag aagtgaaatc aacctttata
     7981 attaagcaga aattacaaaa ctaggaataa ttgacgttgt aagatatgtt aataaaacct
     8041 actgtcattt gctttgtgca agatccttaa aaaaaaaaaa aaaaaa
//
