LOCUS       NM_009461               7664 bp    mRNA    linear   ROD 08-AUG-2024
DEFINITION  Mus musculus ubiquitin protein ligase E3 component n-recognin 1
            (Ubr1), mRNA.
ACCESSION   NM_009461
VERSION     NM_009461.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Zhao,W., Zhang,Y., Lin,S., Li,Y., Zhu,A.J., Shi,H. and Liu,M.
  TITLE     Identification of Ubr1 as an amino acid sensor of steatosis in
            liver and muscle
  JOURNAL   J Cachexia Sarcopenia Muscle 14 (3), 1454-1467 (2023)
   PUBMED   37057345
  REMARK    GeneRIF: Identification of Ubr1 as an amino acid sensor of
            steatosis in liver and muscle.
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Vu,T.T.M. and Varshavsky,A.
  TITLE     The ATF3 Transcription Factor Is a Short-Lived Substrate of the
            Arg/N-Degron Pathway
  JOURNAL   Biochemistry 59 (30), 2796-2812 (2020)
   PUBMED   32692156
REFERENCE   3  (bases 1 to 7664)
  AUTHORS   Piatkov,K.I., Brower,C.S. and Varshavsky,A.
  TITLE     The N-end rule pathway counteracts cell death by destroying
            proapoptotic protein fragments
  JOURNAL   Proc Natl Acad Sci U S A 109 (27), E1839-E1847 (2012)
   PUBMED   22670058
REFERENCE   4  (bases 1 to 7664)
  AUTHORS   Tasaki,T., Zakrzewska,A., Dudgeon,D.D., Jiang,Y., Lazo,J.S. and
            Kwon,Y.T.
  TITLE     The substrate recognition domains of the N-end rule pathway
  JOURNAL   J Biol Chem 284 (3), 1884-1895 (2009)
   PUBMED   19008229
REFERENCE   5  (bases 1 to 7664)
  AUTHORS   Ozawa,Y., Nakao,K., Kurihara,T., Shimazaki,T., Shimmura,S.,
            Ishida,S., Yoshimura,A., Tsubota,K. and Okano,H.
  TITLE     Roles of STAT3/SOCS3 pathway in regulating the visual function and
            ubiquitin-proteasome-dependent degradation of rhodopsin during
            retinal inflammation
  JOURNAL   J Biol Chem 283 (36), 24561-24570 (2008)
   PUBMED   18614536
  REMARK    GeneRIF: A STAT3-dependent E3 ubiquitin ligase, Ubr1, was
            responsible for rhodopsin degradation and was up-regulated in the
            inflamed SOCS3-deficient retinas.
REFERENCE   6  (bases 1 to 7664)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7664)
  AUTHORS   Kwon,Y.T., Kashina,A.S., Davydov,I.V., Hu,R.G., An,J.Y., Seo,J.W.,
            Du,F. and Varshavsky,A.
  TITLE     An essential role of N-terminal arginylation in cardiovascular
            development
  JOURNAL   Science 297 (5578), 96-99 (2002)
   PUBMED   12098698
REFERENCE   8  (bases 1 to 7664)
  AUTHORS   Kwon,Y.T., Xia,Z., Davydov,I.V., Lecker,S.H. and Varshavsky,A.
  TITLE     Construction and analysis of mouse strains lacking the ubiquitin
            ligase UBR1 (E3alpha) of the N-end rule pathway
  JOURNAL   Mol Cell Biol 21 (23), 8007-8021 (2001)
   PUBMED   11689692
REFERENCE   9  (bases 1 to 7664)
  AUTHORS   Kwon,Y.T., Balogh,S.A., Davydov,I.V., Kashina,A.S., Yoon,J.K.,
            Xie,Y., Gaur,A., Hyde,L., Denenberg,V.H. and Varshavsky,A.
  TITLE     Altered activity, social behavior, and spatial memory in mice
            lacking the NTAN1p amidase and the asparagine branch of the N-end
            rule pathway
  JOURNAL   Mol Cell Biol 20 (11), 4135-4148 (2000)
   PUBMED   10805755
REFERENCE   10 (bases 1 to 7664)
  AUTHORS   Kwon,Y.T., Reiss,Y., Fried,V.A., Hershko,A., Yoon,J.K., Gonda,D.K.,
            Sangan,P., Copeland,N.G., Jenkins,N.A. and Varshavsky,A.
  TITLE     The mouse and human genes encoding the recognition component of the
            N-end rule pathway
  JOURNAL   Proc Natl Acad Sci U S A 95 (14), 7898-7903 (1998)
   PUBMED   9653112
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844548.7 and AL935168.11.
            
            On Nov 8, 2022 this sequence version replaced NM_009461.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF061555.1, SRR17253012.10382138.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                AL844548.7         79510-79602         c
            94-350              AL844548.7         72352-72608         c
            351-429             AL844548.7         70073-70151         c
            430-540             AL844548.7         64598-64708         c
            541-671             AL844548.7         59028-59158         c
            672-810             AL844548.7         56746-56884         c
            811-873             AL844548.7         55610-55672         c
            874-997             AL844548.7         55262-55385         c
            998-1105            AL844548.7         54822-54929         c
            1106-1194           AL844548.7         53639-53727         c
            1195-1293           AL844548.7         51465-51563         c
            1294-1451           AL844548.7         49955-50112         c
            1452-1551           AL844548.7         44204-44303         c
            1552-1680           AL844548.7         43292-43420         c
            1681-1861           AL844548.7         39834-40014         c
            1862-1923           AL844548.7         38709-38770         c
            1924-2034           AL844548.7         38374-38484         c
            2035-2100           AL844548.7         35282-35347         c
            2101-2202           AL844548.7         34943-35044         c
            2203-2266           AL844548.7         33120-33183         c
            2267-2391           AL844548.7         30366-30490         c
            2392-2444           AL844548.7         26809-26861         c
            2445-2547           AL844548.7         25412-25514         c
            2548-2596           AL844548.7         24356-24404         c
            2597-2751           AL844548.7         23855-24009         c
            2752-2851           AL844548.7         20986-21085         c
            2852-2961           AL844548.7         20090-20199         c
            2962-3048           AL844548.7         18410-18496         c
            3049-3230           AL844548.7         15832-16013         c
            3231-3436           AL844548.7         12982-13187         c
            3437-3530           AL844548.7         10590-10683         c
            3531-3680           AL844548.7         9489-9638           c
            3681-3778           AL844548.7         5484-5581           c
            3779-3872           AL844548.7         4060-4153           c
            3873-4021           AL844548.7         2056-2204           c
            4022-4077           AL935168.11        180662-180717       c
            4078-4174           AL935168.11        178366-178462       c
            4175-4242           AL935168.11        170811-170878       c
            4243-4393           AL935168.11        169621-169771       c
            4394-4493           AL935168.11        165549-165648       c
            4494-4627           AL935168.11        163956-164089       c
            4628-4736           AL935168.11        161714-161822       c
            4737-4793           AL935168.11        159579-159635       c
            4794-4871           AL935168.11        156816-156893       c
            4872-5042           AL935168.11        154980-155150       c
            5043-5144           AL935168.11        152908-153009       c
            5145-7664           AL935168.11        148862-151381       c
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..7664
                     /gene="Ubr1"
                     /note="ubiquitin protein ligase E3 component n-recognin 1"
                     /db_xref="GeneID:22222"
                     /db_xref="MGI:MGI:1277977"
     exon            1..93
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..5286
                     /gene="Ubr1"
                     /EC_number="2.3.2.27"
                     /note="ubiquitin-protein ligase e3 componen n-recognin;
                     N-recognin-1; ubiquitin-protein ligase E3-alpha-1;
                     ubiquitin-protein ligase E3-alpha-I; E3 alpha; RING-type
                     E3 ubiquitin transferase UBR1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase UBR1"
                     /protein_id="NP_033487.2"
                     /db_xref="CCDS:CCDS16628.1"
                     /db_xref="GeneID:22222"
                     /db_xref="MGI:MGI:1277977"
                     /translation="MADEEMDGAERMDVSPEPPLAPQRPASWWDQQVDFYTAFLHHLA
                     QLVPEIYFAEMDPDLEKQEESVQMSILTPLEWYLFGEDPDICLEKLKHSGAFQLCGKV
                     FKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTG
                     PFCVDHEPGRAGTTKESLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQ
                     IREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVY
                     ATCQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQI
                     FCQACLVEEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEF
                     VKYYKQLQKEYISDDHERSISITALSVQMLTVPTLARHLIEEQNVISVITETLLEVLP
                     EYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPVIWTERLRAQFLEGFRSFLKI
                     LTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKE
                     CHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSR
                     LGAISRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVK
                     CREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELTDAFNKTISTKDQDLIKQYNTLI
                     EEMLQVLIYIVGERYVPGVGNVTREEVIMREITHLLCIEPMPHSAIARNLPENENNET
                     GLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQ
                     ENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMA
                     FHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLE
                     SQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAAR
                     LHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPK
                     RGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDP
                     LFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCK
                     SLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKGEAPAVP
                     VLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPR
                     VPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLI
                     HKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSS
                     YNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDG
                     LAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYL
                     SLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYS
                     CLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHC
                     GAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKL
                     HLVWQQHCIIEEIARSQETNQMLFGFNWQLL"
     exon            94..350
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            351..429
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            430..540
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..671
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            672..810
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            811..873
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            874..997
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1105
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1194
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1293
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1451
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1551
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1680
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1861
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1923
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2034
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2100
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2202
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2266
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2391
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2444
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2547
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2596
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2751
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2752..2851
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2961
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3048
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..3230
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3436
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3530
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3531..3680
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3681..3778
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3779..3872
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4021
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4022..4077
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4078..4174
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4175..4242
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4243..4393
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4394..4493
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4494..4627
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4628..4736
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4737..4793
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4794..4871
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            4872..5042
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            5043..5144
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     exon            5145..7664
                     /gene="Ubr1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7626..7631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ubr1"
                     /note="hexamer: AATAAA"
     polyA_site      7664
                     /gene="Ubr1"
                     /note="major polyA site"
ORIGIN      
        1 gtttccctta agatggcgga cgaagagatg gacggcgccg agaggatgga cgtcagcccg
       61 gagcctcccc tggccccgca gcggccggca tcgtggtggg atcagcaagt tgatttctat
      121 actgctttct tacatcattt ggcacaatta gtgccagaaa tttattttgc tgagatggac
      181 ccagatttgg aaaagcaaga agagagtgta cagatgtcaa tactcactcc tttggagtgg
      241 tacttatttg gagaggatcc ggatatttgc ttagagaaat taaaacacag tggagcgttc
      301 cagttgtgtg ggaaggtttt caaaagtgga gaaacaacat attcctgtag ggattgtgca
      361 attgatccaa cgtgtgtgct ctgtatggac tgcttccaaa gtagtgttca taaaaaccat
      421 cgttacaaga tgcatacttc tactggagga ggcttctgtg actgtggaga cacagaagcg
      481 tggaaaactg gccctttttg tgtggatcac gagcctggaa gagcaggtac tacaaaagag
      541 agcttacatt gcccattgaa tgaagaggtg attgctcaag ccaggagaat attcccttcg
      601 gtgataaaat acattgtaga aatgactata tgggaagaag aaaaggaatt gcctcctgaa
      661 ctgcagataa gggagaaaaa tgaacgatac tattgtgtcc ttttcaacga tgagcaccat
      721 tcgtatgatc atgtgatcta cagtctgcag agagctctag attgcgagct tgcagaggca
      781 cagctgcaca cgactgccat cgacaaagag ggtcgccggg ctgtcaaagc aggtgtgtat
      841 gccacttgcc aggaagcaaa ggaggatata aagagtcact cagagaacgt ctctcagcac
      901 cccctccatg tggaagtgct gcactccgtg gttatggctc accagaaatt cgctctgcgc
      961 cttggctcct ggatgaacaa aattatgagc tattcaagtg actttagaca gatattttgc
     1021 caggcctgcc tcgtagaaga acctggctct gaaaatccct gtcttataag cagactaatg
     1081 ctttgggatg caaaacttta taaaggtgcc cgtaagatcc ttcatgaatt gatctttagt
     1141 agttttttta tggagatgga atacaaaaaa ctctttgcta tggaatttgt gaagtattat
     1201 aaacagctgc agaaagagta catcagcgac gaccacgaga gaagcatctc cataaccgcc
     1261 ctgtccgtgc agatgctcac cgtcccgacc ttggcccggc atcttattga agagcagaat
     1321 gttatttctg tcattactga aacgctgcta gaagttttac ctgaatactt ggacaggaac
     1381 aataaattca acttccaggg ttatagccag gacaaactgg gaagagtcta cgcagttata
     1441 tgtgacctaa agtatatcct gattagcaag cctgtcatat ggacagaacg attaagagcg
     1501 cagttcctgg aaggtttccg gtcttttctg aagattctta cctgtatgca gggaatggaa
     1561 gaaatcagaa gacaagttgg acaacacatt gaagtggacc ctgactggga ggctgccatc
     1621 gctatacaga tgcaactaaa gaatattttg ctcatgttcc aagagtggtg tgcttgtgat
     1681 gaagatctct tactggtggc ttataaagaa tgtcacaaag ctgtaatgag gtgcagtaca
     1741 aatttcatgt ccagtaccaa gacagtagtg caattgtgcg gtcatagtct ggaaaccaaa
     1801 tcctacaaag tgtctgagga ccttgtaagc atacacctgc cactctctag aacacttgct
     1861 ggtcttcatg tacgtttaag cagactaggt gctatttcaa gactgcatga atttgtgcct
     1921 tttgacggct ttcaagtaga ggtcctggtg gagtacccgc tgcgctgcct ggtcctggtg
     1981 gctcaggttg ttgctgagat gtggcgaaga aacgggctct cactcatcag ccaggttttc
     2041 tattatcaag atgttaaatg cagggaggaa atgtacgata aagatatcat catgcttcag
     2101 attggagcat ctataatgga tcccaacaag ttcttgttac tggtacttca gagatatgaa
     2161 cttactgatg cttttaacaa gaccatatcc acaaaagacc aggatttgat taaacagtat
     2221 aatacattaa tagaagaaat gcttcaggtc ctcatctata ttgtgggaga acgttatgta
     2281 cctggagtgg gaaatgttac cagagaggag gttataatga gagagattac tcacttactt
     2341 tgcattgagc ccatgccaca tagtgccatc gccagaaacc tacctgagaa cgaaaataat
     2401 gaaactggct tagagaatgt cataaacaaa gtggccacat ttaagaaacc aggtgtgtcg
     2461 ggccatggag tttatgaatt gaaagatgaa tcactgaaag acttcaatat gtacttttac
     2521 cattattcta aaacacagca tagcaaggct gaacatatgc agaagaaaag gagaaaacaa
     2581 gaaaataaag atgaagcatt gccgccgcca cctcctccgg agttctgccc tgctttcagc
     2641 aaagtagtca acctgctcag ctgtgatgtt atgatgtaca tcctcaggac catctttgag
     2701 cgggcagtgg acatggagtc taatctgtgg acagaaggga tgctgcagat ggcgttccat
     2761 atattggcac tgggcttgct ggaagagaag cagcagcttc agaaagctcc tgaagaggaa
     2821 gtggcttttg acttttacca taaggcttca agattgggaa gttcagccat gaatgctcag
     2881 aatatacaaa tgctcttgga aaaactcaaa ggaatccccc aattagaaag ccagaaggac
     2941 atgataacat ggatactcca gatgtttgac acagtgaagc gattaagaga aaaatcttgt
     3001 ttagttgtgg caaccacttc aggactggag tgcgttaaga gtgaggagat tactcatgat
     3061 aaagaaaagg cagaaaggaa gagaaaagct gaggccgcta ggcttcatcg ccagaagatc
     3121 atggcccaga tgtctgcctt acagaaaaac ttcattgaaa cccacaaact catgtatgat
     3181 aatacgtcag aagtaacagg gaaggaagac tccattatgg aggaagagag cacctcagca
     3241 gtcagtgagg cctctagaat tgctctgggc cctaaacggg gcccggctgt tactgaaaag
     3301 gaggtgctga cgtgcatcct ctgccaagaa gaacaagagg tgaaactaga aaataacgcc
     3361 atggtattgt cagcatgtgt gcagaaatcc accgccctaa cccagcacag agggaagcct
     3421 gtggaccact taggggaaac actggaccct cttttcatgg atccagactt ggcacatgga
     3481 acttatacag gaagctgtgg tcatgtaatg catgcagtgt gctggcagaa gtattttgaa
     3541 gctgtgcagc tgagctcgca gcagcgcatt cacgtagacc tgtttgacct ggagagcggc
     3601 gagtacctat gcccgctctg caagtctctc tgcaatactg tcatccccat catccctttg
     3661 cagccgcaga agatcaacag tgagaatgcg gaggctcttg ctcaactttt gaccttggcc
     3721 cggtggatac agactgtcct tgccagaata tcgggttata atataaagca tgctaaagga
     3781 gaagccccag cagttcctgt cttgtttaat caaggaatgg gggattcgac ttttgagttt
     3841 cattccatcc tgagttttgg agttcagtct tcggtgaaat attcaaatag tatcaaggaa
     3901 atggtcattc tcttcgccac aacaatttac agaattggcc tgaaagtgcc tcctgatgaa
     3961 ctagacccac gagtgcccat gatgacctgg agcacgtgtg cgttcaccat ccaggcaatc
     4021 gaaaacctgt tgggagatga aggaaaacct ctatttggag cacttcaaaa tagacagcat
     4081 aacggtctga aggcgctaat gcagtttgca gttgcacaga ggactacctg ccctcaggtc
     4141 ctgatacaca aacatctggc tcggctcctg tcagttattc ttcctaacct gcaatcagaa
     4201 aatacaccag gccttctgtc cgtggatctc ttccatgttc tggtcggcgc agtcttagcg
     4261 ttcccatcct tgtattggga tgacaccgtg gatctgcagc cgtcaccact tagttcttca
     4321 tataaccacc tctatctctt ccatctgatc accatggcgc acatgcttca gatactcctt
     4381 acaacagata cagatctgtc ttcagggccg ccgcttgctg agggtgaaga ggatagtgag
     4441 gaggctcgct gtgcatctgc tttctttgtg gaagtgtcgc agcacacaga cggcctcgct
     4501 gggtgcggtg ctcccggctg gtacctgtgg ctctccctga ggaacggcat caccccttac
     4561 ctccgctgtg ctgcactgct tttccactat ttacttggag tagctccgcc tgaagaactg
     4621 tttgccaatt ctgctgaagg agaattcagt gcactctgta gctatctatc tttacccaca
     4681 aatttgttcc tgcttttcca ggaatattgg gataccataa ggcccttact acagaggtgg
     4741 tgtggagatc ctgccttact caagtctttg aagcagaaaa gtgctgtggt caggtaccct
     4801 agaaaaagaa atagtttgat agagcttcct gaggactaca gctgtcttct aaatcaggct
     4861 tctcacttta ggtgtccacg gtctgcagat gatgagcgaa agcatcctgt cctctgtctt
     4921 ttctgtgggg ccatcctgtg ttctcagaac atctgttgcc aagaaatagt gaatggggaa
     4981 gaggttggag cgtgcgtttt tcatgcgctt cattgtggtg ctggagtctg cattttctta
     5041 aaaatccgag aatgcagggt ggtcctggtg gaaggaaaag ccagaggctg tgcctaccca
     5101 gccccttact tggatgaata tggagaaaca gacccagggc taaagagagg aaacccactt
     5161 catttatctc gggagcggta tcggaagctg catttggtct ggcaacagca ctgcattata
     5221 gaagagattg ctcggagcca ggagactaat cagatgctat ttggattcaa ctggcagtta
     5281 ctctgagctc cagttctgcc tcaagacaat catgagtgac atcaataata aagactgatc
     5341 taaaattcta gagaactttc tgaggacggg gaagtattgg agggtctttt gatccatgtc
     5401 cagattcaca cacattaata aaatattcct taatggaata ttgctttcaa ttatcaaaca
     5461 taagcttcaa gggaaaaaca agacatagat taatgtttta tgttctagaa cactaaagaa
     5521 atgcttgttc atccaagtgt ctatttctgc taatatttcc agaaaactcc tttcccttca
     5581 taactgtcct agttcatttc atatcaccca cctggttaat gaggtcacat taagcatttg
     5641 tggacatttc tccatctggc taacatctct gcacctttgt atttggtgtt tctcgagtgt
     5701 agtttagctt gggttagatc tgaaaagatg ctgatcacct gtgatggtct aaagaggaat
     5761 tgcacaacta tgcagtttct ttcaattaaa aatttcaaaa catgtaaaca tctttcttct
     5821 ttaaggaaat atccttattg taccacctac ggcttcagtc agaaacagat ctaaatctct
     5881 ctatggagag tgctagctgt gctagtctgg aaagcatcct tccagtatag acctcaagta
     5941 gattcaggag aatgtgctca ttacgcattc cttatacaaa agcctgttat cctcacctga
     6001 ttccagggag ctctgtggag tcacaagttc tccatcagtt acatttctta aggcagattt
     6061 ctgcagtaag atctcgtctc ttggggcccc atcctattgt ctctcagaaa actcttgttt
     6121 tgaagcaaac tctttgtaga atgggaatca gaaaattgcc ccagtgaatg gtcataagag
     6181 atgaaattag aacactgtat ttaagccagt tctgcaacct tctatggctt gtaagaaaca
     6241 ggtccttgat ttgatgtcta ggtgaaacct ttcataaacg actgtttatg attaatataa
     6301 acaacaataa caaaaaagca acgtaatcag gtgtgtgtcc tgttataacc tgctgtggtt
     6361 gagccatctt gcgtataaat aatgcagctc tcctgagtct gtgcatagac ctcttgattc
     6421 ttggctttac cgttttatat caagaccttg tgagtagatc agttgatact tattttagct
     6481 tttgtggtat tgggtaatat tgcagttgaa agatgtgttt ggagattgtc tcttcctttg
     6541 cccttggcta ctgctgccat gtgtgtgtct aagtgttttc ctgccttctg tttagtgaag
     6601 accaggaagt aaaaatacat ttgataagtg atcagtgatg cacatttggg gctaaatttg
     6661 ttgccatttt tatggaatga aaagtgggtt ttgcagtaag aaattggtgt gagcaggcac
     6721 cagactcaca atcatgattt cctacttgga taaatactac tcagaagaaa gcaggaacta
     6781 gatgcaacta taatgaacag ttcacagcaa ttataagaga caaaaatcaa taaattcagt
     6841 atttggcttg tgccatttgc caatatgatt aagaaatttg aacattgccc tttgtttttt
     6901 ctataatttt tttttgagag attgtttagc tctgaaatct gggctagaat aggcaacata
     6961 agatgctcat ttctgcctta gtcatctggc ccccatttgc caaggataaa gactttattt
     7021 ttagccttct tagattcaat ttgtaaaccc tggagctaag ctttcaagct gctaggagga
     7081 ctagctagca ctagccaaag agcctaattc atcactcagt tctgcttctt gagatggaag
     7141 tttctgcact ggaggtagac ttaggtcaca gtgctgcccc agcccacggg atctttaata
     7201 gtattcatca tgacaaatga tttaagtctg ctaagaagtt tacttaagca accaagtcac
     7261 tttatgtagt gtagtgtatg aaagaattgg tttagtggga tggctctgac tctgtgtttt
     7321 taatattggt gaggggctgg caaggatttt aatgatacag taaagataaa catatatctg
     7381 tataattaat ttattctgtt agtgtatggg ctgtgagttc accttttagt ggtcatttgt
     7441 catttcataa caactatgca ttttggttca ctgtgatgat gatctatatt tagtgactgc
     7501 aacatgttta taccactgat tcaaattcca ttcatgatga agttatacag atgatgcata
     7561 tattgatatc ttttattgca aaaatgtaaa ttaaaaactt gtataatgtt cttgtgcttt
     7621 tttgaaataa aatatcttat atgtgtatat ttaaaaagaa aaaa
//
