LOCUS       NM_033526               3324 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus ubiquilin 4 (Ubqln4), transcript variant 1, mRNA.
ACCESSION   NM_033526
VERSION     NM_033526.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Mohan,H.M., Trzeciakiewicz,H., Pithadia,A., Crowley,E.V.,
            Pacitto,R., Safren,N., Trotter,B., Zhang,C., Zhou,X., Zhang,Y.,
            Basrur,V., Paulson,H.L. and Sharkey,L.M.
  TITLE     RTL8 promotes nuclear localization of UBQLN2 to subnuclear
            compartments associated with protein quality control
  JOURNAL   Cell Mol Life Sci 79 (3), 176 (2022)
   PUBMED   35247097
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3324)
  AUTHORS   Hjerpe,R., Bett,J.S., Keuss,M.J., Solovyova,A., McWilliams,T.G.,
            Johnson,C., Sahu,I., Varghese,J., Wood,N., Wightman,M., Osborne,G.,
            Bates,G.P., Glickman,M.H., Trost,M., Knebel,A., Marchesi,F. and
            Kurz,T.
  TITLE     UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance
            by the Proteasome
  JOURNAL   Cell 166 (4), 935-949 (2016)
   PUBMED   27477512
REFERENCE   4  (bases 1 to 3324)
  AUTHORS   Suzuki,R. and Kawahara,H.
  TITLE     UBQLN4 recognizes mislocalized transmembrane domain proteins and
            targets these to proteasomal degradation
  JOURNAL   EMBO Rep 17 (6), 842-857 (2016)
   PUBMED   27113755
  REMARK    GeneRIF: UBQLN4 recognizes not only the defective model substrate
            but also a pool of endogenous defective proteins that were induced
            by the depletion of the SRP54 subunit of the signal recognition
            particle.
REFERENCE   5  (bases 1 to 3324)
  AUTHORS   Hamazaki,J., Hirayama,S. and Murata,S.
  TITLE     Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated
            Proteins and Cellular Homeostasis
  JOURNAL   PLoS Genet 11 (7), e1005401 (2015)
   PUBMED   26222436
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3324)
  AUTHORS   Su,V., Nakagawa,R., Koval,M. and Lau,A.F.
  TITLE     Ubiquitin-independent proteasomal degradation of endoplasmic
            reticulum-localized connexin43 mediated by CIP75
  JOURNAL   J Biol Chem 285 (52), 40979-40990 (2010)
   PUBMED   20940304
  REMARK    GeneRIF: although CIP75 can interact with ubiquitinated cellular
            proteins, its interaction with Cx43 and stimulation of Cx43
            proteasomal degradation does not require the ubiquitination of
            Cx43.
REFERENCE   7  (bases 1 to 3324)
  AUTHORS   Li,X., Su,V., Kurata,W.E., Jin,C. and Lau,A.F.
  TITLE     A novel connexin43-interacting protein, CIP75, which belongs to the
            UbL-UBA protein family, regulates the turnover of connexin43
  JOURNAL   J Biol Chem 283 (9), 5748-5759 (2008)
   PUBMED   18079109
  REMARK    GeneRIF: A novel connexin43-interacting protein, CIP75, which
            belongs to the UbL-UBA protein family, regulates the turnover of
            connexin43
REFERENCE   8  (bases 1 to 3324)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 3324)
  AUTHORS   Matsuda,M., Koide,T., Yorihuzi,T., Hosokawa,N. and Nagata,K.
  TITLE     Molecular cloning of a novel ubiquitin-like protein, UBIN, that
            binds to ER targeting signal sequences
  JOURNAL   Biochem Biophys Res Commun 280 (2), 535-540 (2001)
   PUBMED   11162551
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC145168.5.
            
            On Aug 25, 2022 this sequence version replaced NM_033526.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017686.1, AB040050.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AC145168.5         119851-119999
            150-301             AC145168.5         121425-121576
            302-504             AC145168.5         121877-122079
            505-767             AC145168.5         122744-123006
            768-926             AC145168.5         125705-125863
            927-1152            AC145168.5         129195-129420
            1153-1292           AC145168.5         130457-130596
            1293-1376           AC145168.5         131094-131177
            1377-1492           AC145168.5         131300-131415
            1493-1679           AC145168.5         131778-131964
            1680-3324           AC145168.5         134168-135812
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.87 cM"
     gene            1..3324
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /note="ubiquilin 4"
                     /db_xref="GeneID:94232"
                     /db_xref="MGI:MGI:2150152"
     exon            1..149
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1832
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /note="isoform 1 is encoded by transcript variant 1;
                     connexin43-interacting protein of 75 kDa; ataxin-1
                     interacting ubiquitin-like protein; ataxin-1
                     ubiquitin-like-interacting protein A1U; ataxin-1
                     ubiquitin-like interacting protein"
                     /codon_start=1
                     /product="ubiquilin-4 isoform 1"
                     /protein_id="NP_277068.1"
                     /db_xref="CCDS:CCDS17479.1"
                     /db_xref="GeneID:94232"
                     /db_xref="MGI:MGI:2150152"
                     /translation="MAEPSGAETRPQIRVTVKTPKDKEEIVICDQASVKEFKEEISRR
                     FKAQQDQLVLIFAGKILKDGDTLSQHGIKDGLTVHLVIKTPQKAQDPVTAAASPPSTP
                     DSASAPSTTPASPAAAPVQPCSSGNTTSDAGSGGGPSPVAAEGPSSATASILSGFGGI
                     LGLGSLGLGSANFMELQQQMQRQLMSNPEMLSQIMENPLVQDMMSNPDLMRHMIMANP
                     QMQQLMERNPEISHMLNNPELMRQTMELARNPAMMQEMMRNQDRALSNLESVPGGYNA
                     LRRMYTDIQEPMFTAAREQFGNNPFSSLAGNSDNSSSQPLRTENREPLPNPWSPSPPT
                     SQAPGSGGEGTGGSGTSQVHPTVSNPFGINAASLGSGMFNSPEMQALLQQISENPQLM
                     QNVISAPYMRTMMQTLAQNPDFAAQMMVNVPLFAGNPQLQEQLRLQLPVFLQQMQNPE
                     SLSILTNPRAMQALLQIQQGLQTLQTEAPGLVPSLGSFGTPRTSVPLAGSNSGSSAEA
                     PTSSPGVPATSPPSAGSNAQQQLMQQMIQLLSGSGNSQVPMPEVRFQQQLEQLNSMGF
                     INREANLQALIATGGDINAAIERLLGSQLS"
     exon            150..301
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            302..504
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            505..767
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            768..926
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1152
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1292
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1376
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1492
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1679
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..3324
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3294..3299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /note="hexamer: AATAAA"
     polyA_site      3324
                     /gene="Ubqln4"
                     /gene_synonym="A1u; A1Up; CIP75; UBIN"
                     /note="major polyA site"
ORIGIN      
        1 gaggaggagc cggacgggct ggcgggcggc cgggcggcgg catggcggag ccgagtgggg
       61 ctgagacgag gccccagatt cgggtcaccg tcaagacccc caaggacaag gaggagatcg
      121 tgatctgcga tcaagcctcg gtcaaggagt tcaaggagga gatctcccgg aggtttaagg
      181 ctcagcagga tcagctggtc ctgatcttcg caggcaagat cctcaaggat ggggacaccc
      241 tgagccagca cggcatcaag gatgggctca cagtccacct agtcatcaaa acccctcaga
      301 aggctcaaga tccagtgact gctgctgctt ctcctccatc cactcctgac tctgcctctg
      361 caccctccac cacgcctgcc tcacctgccg ccgcccctgt ccagccctgc agctctggca
      421 atacaacttc agatgctggc agtggagggg ggccctctcc agtggctgca gaggggccct
      481 caagtgctac tgcatcgatc ctctctggct tcggaggcat cctgggactg ggcagcctgg
      541 gcctgggctc cgccaacttt atggagctgc agcaacagat gcagagacag ctcatgtcca
      601 atcctgagat gctgtcgcag atcatggaga accccttggt ccaggatatg atgtcaaacc
      661 ctgatctgat gcgccacatg atcatggcta atccccagat gcagcagctg atggagagga
      721 accccgagat cagccacatg ctcaacaacc cagagctcat gaggcagaca atggagcttg
      781 ctcgtaaccc agccatgatg caagaaatga tgcggaacca ggaccgggcc ctcagcaacc
      841 tggaaagtgt cccgggaggg tataatgccc tccgccgcat gtacacggac atccaggagc
      901 ccatgttcac tgctgctcgg gaacagtttg gcaacaatcc cttctcttcc ctggccggga
      961 actccgacaa ctcatcctcc caacctcttc ggactgagaa tcgagagccc ctccctaacc
     1021 cctggagccc ctcgcccccc acctcccagg cccccgggtc cggtggggag ggcaccggag
     1081 gatcggggac cagccaggtg cacccgacag tctcgaaccc ctttgggatc aatgcggcta
     1141 gcctggggtc agggatgttc aacagcccag aaatgcaggc gctcctccag cagatttctg
     1201 aaaaccccca gctgatgcag aatgtcatct cagcccctta catgcgcacc atgatgcaga
     1261 cgcttgccca gaaccctgac tttgctgctc agatgatggt gaatgtgccg ctgttcgcag
     1321 ggaatccaca gcttcaggag cagctccgcc tgcagctccc cgtgtttctg cagcagatgc
     1381 aaaacccaga gtctctctcc atcctcacca atccccgggc catgcaggcc ctgctgcaga
     1441 tccagcaggg actacagacc ctccagactg aagcccctgg gctggtaccc agccttggct
     1501 cctttgggac accccggacc tcggtacctc tggcaggcag caactctgga tcttcggcag
     1561 aggcccccac ttcttccccg ggcgtgccag ccacatctcc tcccagtgcg ggttccaatg
     1621 cccagcaaca actcatgcag cagatgatcc aacttttgtc cggaagtggg aactcacagg
     1681 tgccgatgcc agaagtgaga tttcaacagc agctggaaca actcaactcc atgggcttta
     1741 ttaaccgtga ggccaaccta caggccctga tcgccacagg aggggacatc aatgccgcta
     1801 ttgagagact cctgggctcc cagctctcct gatcccttgg ctggtgcctc ctgccttccc
     1861 cacctctcag cgccagcctc tggctcctct gtgaagccct atcttccatg gcctgtcttc
     1921 tttgacccct acgaacagag cagcaagaac cgcagggtga cttccaactt ccaaggcctc
     1981 tgcccaaccc aaagctctcc aaggattttg attcgtttac tgtctcatga aatctctccc
     2041 ggtcctcttg gagcagagct atctagtgtg cacagtgtgc cagtttggcc acatctcctt
     2101 gcccacaagt accttggcaa catacattct tctccagggg actgagagga ggaccccgcc
     2161 tggcgtaccg gaagtcttcc gttggtacca ctagagttta gctagcctta cattactcct
     2221 accctcctct ccctccctca cctcagccca accagtttga atctcactct ttggccaggt
     2281 cctggtgaaa ctgttttttt tttctctacc cctatactgt ttcatatagc ctgtccttct
     2341 gtccaaaggc agcagtgagt cagcagtctg ggtagggtta tcaagatcaa accagaccag
     2401 tggacactgg tgctctagag ccacagctga gagctctcgg aagcagggac ccggggagac
     2461 agcctgggca ggaggcttgg aagttggagt tgacagagtc tagaaagacc aatagatttc
     2521 atggtggaga agccaggctc aggcctgaca gaagaaagtc tgatggactt ggaactgaag
     2581 atagaagttc agcaaagctg aacagtgtga cccatgagaa atcaaattgg ctccattgtt
     2641 tccagacata tttgcgctca cgttccaatg aacccagtct tcaggctcca gtattttgag
     2701 ccccactctc cagaagatct ggtggggttt ctcagcattg tagaaaatct atatgtgtgc
     2761 gtacatgcaa gttggggagg tgtgtgttct ttgttgattt ctggttcaca actttgtcaa
     2821 cagagataag tgggtacaag ggccaatctt gtggaagact atagcattga gggagccggg
     2881 tccctgggcc tctgggtatt gggtaaccag aaagcagatt gagtaattct ctgagcaata
     2941 atggctctga ctggtaccct ggccaccatt gcttccttgg tgaaagaata gtgaggtttc
     3001 tcttccagag ctcctgcctt tcagttattt gctggttccc tctaggccag aaacttgtat
     3061 ttgaggagag gtgggatggc agatgccttt ggtatggaac tgggctttgc ttaacgagta
     3121 ccagaggaag ctctcgccac attttctctg ctgtgtggtc caggtacttg gaggacccct
     3181 ggctgagaga caggtaagga aaggcatcct gccgtaggtg ggggacagtc ccagacagtt
     3241 cagacatgcc ttcccttccc cccttatacc caacacccac tctggccttt gtaaataaat
     3301 aatgtgattt ttgttgtgag atta
//
