LOCUS       NM_011668               9224 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin protein ligase E3A (Ube3a), transcript
            variant 2, mRNA.
ACCESSION   NM_011668
VERSION     NM_011668.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Vihma,H., Li,K., Welton-Arndt,A., Smith,A.L., Bettadapur,K.R.,
            Gilmore,R.B., Gao,E., Cotney,J.L., Huang,H.C., Collins,J.L.,
            Chamberlain,S.J., Lee,H.M., Aube,J. and Philpot,B.D.
  TITLE     Ube3a unsilencer for the potential treatment of Angelman syndrome
  JOURNAL   Nat Commun 15 (1), 5558 (2024)
   PUBMED   38977672
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Gardner,Z., Holbrook,O., Tian,Y., Odamah,K. and Man,H.Y.
  TITLE     The role of glia in the dysregulation of neuronal spinogenesis in
            Ube3a-dependent ASD
  JOURNAL   Exp Neurol 376, 114756 (2024)
   PUBMED   38508482
  REMARK    GeneRIF: The role of glia in the dysregulation of neuronal
            spinogenesis in Ube3a-dependent ASD.
REFERENCE   3  (bases 1 to 9224)
  AUTHORS   Jana,S., Giri,B., Das,S., Manna,A., Mandal,S.C. and Ranjan Jana,N.
  TITLE     Azadiradione up-regulates the expression of parvalbumin and BDNF
            via Ube3a
  JOURNAL   Gene 897, 148081 (2024)
   PUBMED   38101713
REFERENCE   4  (bases 1 to 9224)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 9224)
  AUTHORS   Tian,Y., Yu,F., Yun,E., Lin,J.W. and Man,H.Y.
  TITLE     mRNA nuclear retention reduces AMPAR expression and promotes
            autistic behavior in UBE3A-overexpressing mice
  JOURNAL   EMBO Rep 25 (3), 1282-1309 (2024)
   PUBMED   38316900
  REMARK    GeneRIF: mRNA nuclear retention reduces AMPAR expression and
            promotes autistic behavior in UBE3A-overexpressing mice.
REFERENCE   6  (bases 1 to 9224)
  AUTHORS   Yamasaki,K., Joh,K., Ohta,T., Masuzaki,H., Ishimaru,T., Mukai,T.,
            Niikawa,N., Ogawa,M., Wagstaff,J. and Kishino,T.
  TITLE     Neurons but not glial cells show reciprocal imprinting of sense and
            antisense transcripts of Ube3a
  JOURNAL   Hum Mol Genet 12 (8), 837-847 (2003)
   PUBMED   12668607
  REMARK    GeneRIF: Ube3a exhibits brain cell type-specific imprinting, with
            monoallelic expression from the maternal allele in neurons, but
            biallelic expression in glial cells. The antisense Ube3a transcript
            is reciprocally imprinted only in neurons.
REFERENCE   7  (bases 1 to 9224)
  AUTHORS   Sutcliffe,J.S., Jiang,Y.H., Galijaard,R.J., Matsuura,T., Fang,P.,
            Kubota,T., Christian,S.L., Bressler,J., Cattanach,B.,
            Ledbetter,D.H. and Beaudet,A.L.
  TITLE     The E6-Ap ubiquitin-protein ligase (UBE3A) gene is localized within
            a narrowed Angelman syndrome critical region
  JOURNAL   Genome Res 7 (4), 368-377 (1997)
   PUBMED   9110176
REFERENCE   8  (bases 1 to 9224)
  AUTHORS   Dhar,M.S. and Johnson,D.K.
  TITLE     A microsatellite map of the pink-eyed dilution (p) deletion complex
            in mouse chromosome 7
  JOURNAL   Mamm Genome 8 (2), 143-145 (1997)
   PUBMED   9060416
  REMARK    Erratum:[Mamm Genome 1997 May;8(5):386]
REFERENCE   9  (bases 1 to 9224)
  AUTHORS   Johnson,D.K., Stubbs,L.J., Culiat,C.T., Montgomery,C.S.,
            Russell,L.B. and Rinchik,E.M.
  TITLE     Molecular analysis of 36 mutations at the mouse pink-eyed dilution
            (p) locus
  JOURNAL   Genetics 141 (4), 1563-1571 (1995)
   PUBMED   8601494
REFERENCE   10 (bases 1 to 9224)
  AUTHORS   Huibregtse,J.M., Scheffner,M., Beaudenon,S. and Howley,P.M.
  TITLE     A family of proteins structurally and functionally related to the
            E6-AP ubiquitin-protein ligase
  JOURNAL   Proc Natl Acad Sci U S A 92 (7), 2563-2567 (1995)
   PUBMED   7708685
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1995 May 23;92(11):5249. doi:
            10.1073/pnas.92.11.5249-b.. PMID: 7761480]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029133.1, CJ125878.1, AK220549.1, CD352481.1, BC094605.1 and
            AC167971.3.
            
            On Mar 28, 2018 this sequence version replaced NM_011668.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U96636.1, SRR7345562.3236374.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 12668607
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                AK029133.1         2-29
            29-102              CJ125878.1         28-101
            103-522             AK220549.1         1-420
            523-709             CD352481.1         418-604
            710-5099            AK220549.1         421-4810
            5100-8673           BC094605.1         364-3937
            8674-8677           AC167971.3         38470-38473         c
            8678-9224           BC094605.1         3942-4488
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 33.95 cM"
     gene            1..9224
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /note="ubiquitin protein ligase E3A"
                     /db_xref="GeneID:22215"
                     /db_xref="MGI:MGI:105098"
     exon            1..522
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            523..589
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            590..709
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     CDS             690..3302
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /EC_number="2.3.2.26"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitin conjugating enzyme E3A; oncogenic
                     protein-associated protein E6-AP; E6-AP ubiquitin protein
                     ligase; HECT-type ubiquitin transferase E3A"
                     /codon_start=1
                     /product="ubiquitin-protein ligase E3A isoform 2"
                     /protein_id="NP_035798.2"
                     /db_xref="CCDS:CCDS39973.1"
                     /db_xref="GeneID:22215"
                     /db_xref="MGI:MGI:105098"
                     /translation="MATACKRSPGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGN
                     EACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAS
                     NNSEIKMNKKEGKDFKDVIYLTEEKVYEIYEFCRESEDYSPLIRVIGRIFSSAEALVL
                     SFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNN
                     VQKLGPDDVTVDIDAIRRVYSSLLANEKLETAFLNALVYLSPNVECDLTYHNVYTRDP
                     NYLNLFIIVMENSNLHSPEYLEMALPLFCKAMCKLPLEAQGKLIRLWSKYSADQIRRM
                     METFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDD
                     EEPIPESSELTLQELLGDERRNKKGPRVDPLETELGVKTLDCRKPLISFEEFINEPLN
                     DVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSL
                     VQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVS
                     KEFFQLVVEEIFNPDIGMFTYDEATKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCIL
                     DVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGSVEDDMMITFQISQTDL
                     FGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESP
                     LKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKR
                     LFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKER
                     LLKAITYAKGFGML"
     exon            710..754
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            755..1044
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..2291
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2436
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2642
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2807
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2963
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3037
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3181
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3182..9209
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9188..9193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /note="hexamer: ATTAAA"
     polyA_site      9209
                     /gene="Ube3a"
                     /gene_synonym="4732496B02; 5830462N02Rik; A130086L21Rik;
                     Hpve6a"
                     /note="major polyA site"
ORIGIN      
        1 ctgtcgggat actcggtccg cccacctagt cctctcgtcc agtgctgcgt tcgcgagatc
       61 cgtatttctc ccaagatggt ggcgctcctc tttgggtgac tccaggagac gacagggcct
      121 ttcgtctttg ccagcacctc gtcgcccctc ctgcgctcgc tctctcgctc gcgcaccggg
      181 ccacgcagct gttcaccgcc tcgttacgct tctcttccgt cgacctgtcg ctgacggtgg
      241 cgcctccttc tgcttctctt cggagttgct cgccgccctc gccccccact gtggacagat
      301 cgcgacagca gcgcttcagc gccgacttca aggttgccca ggcgcctggc ctctcggcct
      361 cggtttcctg aggagaagcg cgggtcccgc atgagacccg gcggtggcgc cagcgaaagg
      421 gaacgaggcg gtggcgggcg gcggcggtgg acgagggcga caaggaccag tgaggcggcc
      481 gcagctgcga gggccgcagc ccacgcgcgg gggcgaggac aggttaaaaa atctctctaa
      541 gagcctgatt ttagagttca ccagctcctc agaagtttgg cgaaatatga attattaagc
      601 ctacgttcag atcaagttag cagctagact ggtgtgacaa cctgttttta atcagtgact
      661 caaagctgtt atcaccctga tgtcaccgaa tggccacagc ttgtaaaaga tcaccaggag
      721 aatcccagtc tgaggacatt gaagctagcc gaatgaagcg agcagctgca aagcatctaa
      781 tagaacgcta ctaccatcag ttaactgagg gctgtggaaa tgaggcctgc acgaatgagt
      841 tttgtgcttc ctgtccaact tttcttcgta tggataacaa tgcagcagct attaaagccc
      901 ttgagcttta taaaattaat gcaaaactct gtgatcctca tccctccaag aaaggagcaa
      961 gctcagctta ccttgagaac tcaaaaggtg catctaacaa ctcagagata aaaatgaaca
     1021 agaaggaagg aaaagatttt aaagatgtga tttacctaac tgaagagaaa gtatatgaaa
     1081 tttatgaatt ttgtagagag agtgaggatt attccccttt aattcgtgta attggaagaa
     1141 tattttctag tgctgaggca ctggttctga gctttcggaa agtcaaacag cacacaaagg
     1201 aggaattgaa atctcttcaa gaaaaggatg aagacaagga tgaagatgaa aaggaaaaag
     1261 ctgcatgttc tgctgctgct atggaagaag actcagaagc atcttcttca aggatgggtg
     1321 atagttcaca gggagacaac aatgtacaaa aattaggtcc tgatgatgtg actgtggata
     1381 ttgatgctat tagaagggtc tacagcagtt tgctcgctaa tgaaaaatta gaaactgcct
     1441 tcctgaatgc acttgtatat ctgtcaccta acgtggaatg tgatttgaca tatcataatg
     1501 tgtatactcg agatcctaat tatctcaatt tgttcattat tgtaatggag aatagtaatc
     1561 tccacagtcc tgaatatctg gaaatggcgt tgccattatt ttgcaaagct atgtgtaagc
     1621 taccccttga agctcaagga aaactgatta ggctgtggtc taaatacagt gctgaccaga
     1681 ttcggagaat gatggaaaca tttcagcaac ttattaccta caaagtcata agcaatgaat
     1741 ttaatagccg aaatctagtg aatgatgatg atgccattgt tgctgcttca aagtgtttga
     1801 aaatggttta ctatgcaaat gtagtgggag gggatgtgga cacaaatcat aatgaggaag
     1861 atgatgaaga acccatacct gagtccagcg aattaacact tcaggagctt ctgggagatg
     1921 aaagaagaaa taagaaaggt cctcgagtgg atccactaga aaccgaactt ggcgttaaaa
     1981 ctctagactg tcgaaaacca cttatctcct ttgaagaatt cattaatgaa ccactgaatg
     2041 atgttctaga aatggacaaa gattatacct ttttcaaagt tgaaacagag aacaaattct
     2101 cttttatgac atgtcccttt atattgaatg ctgtcacaaa gaatctggga ttatattatg
     2161 acaatagaat tcgcatgtac agtgaaagaa gaatcactgt tctttacagc ctagttcaag
     2221 gacagcagtt gaatccgtat ttgagactca aagtcagacg tgaccatatt atagatgatg
     2281 cactggtccg gctagagatg attgctatgg aaaatcctgc agacttgaag aagcagttgt
     2341 atgtggaatt tgaaggagaa caaggagtag atgagggagg cgtttccaaa gagttttttc
     2401 agttggttgt ggaggaaatt tttaatccag atattggtat gttcacatat gatgaagcta
     2461 cgaaattatt ttggtttaat ccatcttctt ttgaaactga gggtcagttt actctgattg
     2521 gcatagtcct gggtctggct atttacaata attgtatact ggatgtccat tttcccatgg
     2581 ttgtatacag gaagctaatg gggaaaaaag gaacctttcg tgacttggga gactctcacc
     2641 cagttttata tcagagttta aaggatttat tggaatatga agggagtgtg gaagatgata
     2701 tgatgatcac tttccagata tcacagacag atctttttgg taacccaatg atgtatgatc
     2761 taaaagaaaa tggtgataaa attccaatta caaatgaaaa caggaaggaa tttgtcaatc
     2821 tctattcaga ctacattctc aataaatctg tagaaaaaca attcaaggca tttcgcagag
     2881 gttttcatat ggtgactaat gaatcgccct taaaatactt attcagacca gaagaaattg
     2941 aattgcttat atgtggaagc cggaatctag atttccaggc actagaagaa actacagagt
     3001 atgacggtgg ctatacgagg gaatctgttg tgattaggga gttctgggaa attgttcatt
     3061 cgtttacaga tgaacagaaa agactctttc tgcagtttac aacaggcaca gacagagcac
     3121 ctgttggagg actaggaaaa ttgaagatga ttatagccaa aaatggccca gacacagaaa
     3181 ggttacctac atctcatact tgctttaatg tccttttact tccggaatat tcaagcaaag
     3241 aaaaacttaa agagagattg ttgaaggcca tcacatatgc caaaggattt ggcatgctgt
     3301 aaacaaaaag aaaaagaaaa agaaaaagaa aaagttaaaa aataaatata agagggataa
     3361 tttgatggta atagtatccc agtacaaaaa ggctgtaaga tagtgaacca cagtagtcat
     3421 ctatgtctgt gcctcccttc ttcattgggg acattgtggg ctggaacagc agatttcagc
     3481 tgcatatatg aacaaatcct ttattattat tataattatt tttttgcgtg aaagtgttac
     3541 atattctttc acttgtatgt acagagaggt tttctgaata tttattttaa gggttaaatc
     3601 acttttgctt gtgtttatta ctgcttgagg ttgagccttt ttgagtattt aagatatata
     3661 taccaacgaa actattctcg caaggaaaac attgccacca tttgtagaac atgtaatctt
     3721 caagtatgtg ctattttttg tccctgtatc taagtcaaat caggaacttt tttctaacaa
     3781 tttgcttttg aaacttgaag tcaaggaaac agtgtggtgc aagtactgct gttctagccc
     3841 ccaaagagtt ttctgtacaa aattttgaga accaataaag atggaaggga gaacttggaa
     3901 tgtttgaacc acagccctca gaactttagt aacagcacaa caaattaaaa caactcatgc
     3961 cacagtatgt tgtcttcatg tgtcttgcaa tgaactgttt cagtagccaa tcctcttagt
     4021 atatgaaagg acagggattt tttttttgtt tttgttgttg ttgttgttgt tgttgttttt
     4081 gttgttgttg ttgtttttgt tgtttaagtt tactggggaa agtgcatctg gccaaatgat
     4141 aggatagtca agcctattgc aacaaaatta ggaagtttgt tgtataaata agcatgtaaa
     4201 agtgcactta aaatgaatct ttattattgc tgagatttta atagacaatc caaagtctcc
     4261 ccttctgttg ccgtcatctt gtttaatcaa ccatttttca aggcactcga tcagtgttgc
     4321 agcataacag aaagtacagc tactgtgcct tgtgttactt atttacacag ttagcaggcc
     4381 tggaaatgaa tggaactagt actcctgaga aataaattgt atatccccca aattaaaatt
     4441 tacttcaaag gtgttaaaga tttcatgtcc tatattaaag tacaaatagg cttaaattac
     4501 tggatattta atgtagtttc ccatccctag tcttctatgt ctgtgatgtt aatttctttt
     4561 gttgcataac aaaataaaag aattatgtat ttttaactaa ggagagacat actggtatat
     4621 cattttacta caagctacag ataacctgtt gagcttgtgc cttgattgtt ttaacaacta
     4681 gtgcaaatca acctgatgat tttaattggc aggggataat ggtagctttc aaatcattgg
     4741 aaggggaaaa ggatgtctta ggattatttt ctttcttgta gtagttgaga cagagctctt
     4801 atttactgta atgctaaatg aaacagtggc ttaaatattt taatgggaaa agagaacaca
     4861 gtgcgttcca tattgtgata aggtaacgtg aggttttttt gttttgtttt gttttctttt
     4921 ttttttttct gagctagcct ttagaacact gttgtggtat gtatgctacc ttgattatag
     4981 gaccccctaa atgtgactat agtcatctta atgggcatct tgtccactgt gcttcttatg
     5041 tattatgaaa gtgataagaa gacaaattaa gtgggtatat tttataaaat aaattcatga
     5101 agagatttgt gttcttcagt ttattgtcac tacagtattg aagtagttgt gatactatat
     5161 ctgtgtgaaa gttttcaaat gataatatag attatcaaac agaagtaaca tcaatatatt
     5221 taaaagaaag tgaaaaaaaa atcctaaaaa gttttcagag gtaaaaaaaa aaactctcac
     5281 ttgcttccta atcagttttt ctcggcatgt taccacatta atatggctgc cttttcatga
     5341 ttgctttact agattgtcat ggaacaatac ttacgagtta aaaaatacct tttagtagtg
     5401 tagaacattt caaacttatg gcataaaagc acagcctgca gcaaaacttc tctggaaact
     5461 tggcagctgc agcaaacaaa ttgagtgtac atatcgtggt ctatagttaa ttgtgtgatc
     5521 aaattcattt gcaataacct tacctttatt tggaaataca tcctgtgtag gaatgtatat
     5581 atcaaaagaa acctataaat gagccttcag agaacttgtt cacattaaca tgtggcaaat
     5641 gcatcaacta ggcagcatta ctccgtgttt ccataaggtg gctattgctg aaatcaacct
     5701 cttttaaaga atttttttct caagaatcca cataccctag agaaagaagt gttttactga
     5761 ggctactctt taagatgttc attctttaac tgcttatccc agggtctatt ttaatccttt
     5821 ctacccttcc cagaataaaa taataataat aataataata atagtaactt ccatttgctg
     5881 cttctttcat ctttagagtg taaaacagtt cctggtgttt ggtaggaaaa atgtttacac
     5941 aatatagaca gaggcccttt ttttttcccc tcttctactt tcaaataaga aaattgactt
     6001 tggtaacagt tgtaagaata tttgagtgca tgtatgtact gcatttcact atgtaaaggc
     6061 tgctaatcca ggaatatttc actgaaaatg tacttgcaat aatactagta gtctccattt
     6121 gccacagtaa agaagtcttg tttttaaccc ttgagagcct tgcagagttg ggctacagct
     6181 tcctgaaagg ctcacttttc catcccaact catagacacc ttgtatggat actctcctga
     6241 ttgtacttag aagtactcct ctgaacttcc tgttcaaccc aaagtttttt ctcttgcctc
     6301 atattttgtg ggtctcatat ctcatatcat atcagacccg tgtttccagg acatcatcct
     6361 ggtacctcta agtcattata tagtacaaaa cagtaccgat aaaaacaaaa ttcttactct
     6421 tgtatctcct agttatttct tgttacttcc tttagaactg tcaatttttt ggcctgggga
     6481 gatggcttag tgggtaaagg tgctcactat ataaaacttg acctgaagac tctgcctaga
     6541 ctccacataa acatggaagg aaagagcaac tccacaaagt tgtttcctgg cctttccatg
     6601 tttaccatag cttttgctgt ttcctaccct ctcacagttt atgtgcatat atatgcatgt
     6661 tgtcagttat tgtctctcag acctatttaa ttgtacttta tttcaggccc caatcctttg
     6721 atttactcag gttatcttcc tgactctgaa ctacttctaa ttcttttcat tctcaaaaag
     6781 catttttttt tctgatttgt atgagtactt cccctttgaa tagtttgtac aggtggattt
     6841 ctgcataatt tactagtgca tcctgttcag catccctttc atcactactc aaaattcctt
     6901 tttagattaa ctttatacaa ctcacatatt ctttcagttg gaattttgaa agtatcatac
     6961 tcataaagac ttctattagt tacagtgtgc cttcatagtg taatagcagc ctacatatcc
     7021 ctggtcacag cctggtggat catatttatt tgaacagttg aggaggcctt cagaagtgcg
     7081 ttcccttcct tctttcctgt gttttagctt tgatattgtc ccactattat tattattttt
     7141 tttaaagcat cttctctttt gtatggaatc tgagtaaatt cacagtggaa actaccttgt
     7201 actgatcttt ctgtctctta ggcagtaggc agataagcat taatcaagaa atatacacat
     7261 acatttattg aatagagttt ctggaatcac tgtattatat ctgccccagg gtaaaaaaga
     7321 cagtaagttg gaaaagacta gatggttgaa agcaagacag ttttgtaact ctacatcctg
     7381 tcttctggtg tactcttgac caccgtcttt atgccactca tactcttcca gcagtcatgg
     7441 caactgatac cttaattcgt tctgttatta ctgactagtc tctcaaattt tcatagagga
     7501 atcctccaga tgctgttctc tgtaaatata tgtgagtcat atttaacatt ttcttatact
     7561 ttgctgctca aattcctgta aattctgaca agaattttca atgactgcta aagatctgtg
     7621 cacatggtcc ttcattttca caaagccaat aaatatctgt aacttgagag gttatacacc
     7681 ttactaaaag tcatacaggc aggaaaaggg atgacaaatg gtaaggttgc gtccacatga
     7741 ccacctaaaa tttgtcatct tcctttttgc aaaaacaaac atgactaaat taaacaacaa
     7801 attaagatta ttatgctgac atgcatggaa ccttggatca ttattatgat ttttcattac
     7861 ccagaaacac atagaaaatg ataaggaatg tatctggaat atagtacaca ctggttgggg
     7921 ttgtagcctt cagtcaaatg actctgaatt ttagtttggc actgccattc ccttgacttg
     7981 gattataccc actcctacct tatctctggt tgtgtggttt cagtgaaatt accatgcagc
     8041 tggtagataa catcttggaa gacatcctga gaacagttct cccaacgttg cttgttaact
     8101 taaaaaaaga gagagagaga ttagaaatgt ctgtaatgaa gacttatttt ctaaaacctg
     8161 accttatatc gtccaccttt aaatggaaga gatgtgtttt ctcacatctt ttagatataa
     8221 ttcacatagt ataaaaattc gccattttaa aaagtgtaaa atttgtggtt atagtatatt
     8281 cacagagttg tgaaattgtc tttataaaat tccagagcat ttcatcgctg taaaaagttt
     8341 tctgtaccca ttaagagtga ctgcctgttc ttaactccaa cccctggcaa ctactcgtct
     8401 tcgtatgcct atgaatttgc ctattttggt catttcatgt taatggagtc atacaattat
     8461 gtggcctttt tgtctgtttc gccaccttcc acaaagcaat gagttcaaac tgcatcaatg
     8521 ttgtagcatg tactggtact gtttcttttg tggctgaata ttgtcattgt attgatatgc
     8581 tgtatttttt tgcttgtcca cttggttgtg catacatgtg ttctttctgc tttttgcata
     8641 ttatgaatac aactatgaac actttgtgta catgttttca tttctcctac tgtcttttaa
     8701 gtatcttatg gttttggttc ttaaatttag gtctttgatt catttttgca tgtggtacat
     8761 tttagggatc cagtttcatt ctctaatatg tggatatcta gtagaacaat acctaattgt
     8821 tacaaagact attagtaatt tccttatgag taggcttgct atctttgtca aaaacacgta
     8881 caaatgacca taaatatggc taattttgga ttcttgaact ttgtcccatt gtcctatgtg
     8941 tgtctcttcc tatgccagta ccatactgtc ttaattactg tagccctaga gtaagttttg
     9001 aatttaggat gtgttagttc tctagctttg ttctacatca agataatttt acctgttctg
     9061 ggtcccttgc attccacatg gattttagaa tctatttgtt aatttctaca taaaaagcac
     9121 ctgtgatttt gattgttctt gtgttgaatc gatacatcag attgacgtat gttggtattt
     9181 taacagtatt aaattttata atccataaaa aaaaaaaaaa aaaa
//
