LOCUS       NM_025773               3092 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2W (putative) (Ube2w),
            transcript variant 1, mRNA.
ACCESSION   NM_025773 XM_484835 XM_886402 XM_896807 XM_896814 XM_896827
            XM_906611 XM_919949 XM_919963 XM_919973 XM_919983 XM_989558
            XM_989587 XM_989620 XM_989642 XM_989671
VERSION     NM_025773.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Wang,S., Cui,L., Li,H., Liu,X., Li,X. and Wang,X.
  TITLE     [UBE2W overexpression promotes proliferation of intestinal mucosal
            cells in mice with chemically induced colitis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (12), 2023-2028 (2023)
   PUBMED   38189387
  REMARK    GeneRIF: [UBE2W overexpression promotes proliferation of intestinal
            mucosal cells in mice with chemically induced colitis].
REFERENCE   3  (bases 1 to 3092)
  AUTHORS   Wang,S., Pu,J., Li,X., Yan,Z., Li,C., Zheng,Y., Luo,Z. and Cui,L.
  TITLE     UBE2W Improves the Experimental Colitis by Inhibiting the NF-kappaB
            Signaling Pathway
  JOURNAL   Dig Dis Sci 67 (12), 5529-5539 (2022)
   PUBMED   35314916
  REMARK    GeneRIF: UBE2W Improves the Experimental Colitis by Inhibiting the
            NF-kappaB Signaling Pathway.
REFERENCE   4  (bases 1 to 3092)
  AUTHORS   Lei,B., Xie,L., Zhang,S., Lv,D., Shu,F. and Deng,Y.
  TITLE     UBE2W down-regulation promotes cell apoptosis and correlates with
            hypospermatogenesis
  JOURNAL   Andrologia 52 (1), e13474 (2020)
   PUBMED   31710394
  REMARK    GeneRIF: Expression levels of UBE2W in mouse testes were gradually
            elevated from 2 to 10 weeks, while were significantly deceased in
            the testes with hypospermatogenesis. When UBE2W expression was
            successfully down-regulated in spermatogenic cells, the rate of
            apoptosis was significantly increased and the P53/Bcl-2/caspase
            6/caspase 9 signal pathways were activated.
REFERENCE   5  (bases 1 to 3092)
  AUTHORS   Wang,B., Zeng,L., Merillat,S.A., Fischer,S., Ochaba,J.,
            Thompson,L.M., Barmada,S.J., Scaglione,K.M. and Paulson,H.L.
  TITLE     The ubiquitin conjugating enzyme Ube2W regulates solubility of the
            Huntington's disease protein, huntingtin
  JOURNAL   Neurobiol Dis 109 (Pt A), 127-136 (2018)
   PUBMED   28986324
  REMARK    GeneRIF: Study shows the absence of ubiquitin conjugating enzyme
            Ube2W in HdhQ200 knock-in mice increases levels of soluble
            monomeric mutant huntingtin while reducing insoluble oligomeric
            species. This study sheds light on the potential function of the
            non-canonical ubiquitin-conjugating enzyme, Ube2W, in this polyQ
            neurodegenerative disease.
REFERENCE   6  (bases 1 to 3092)
  AUTHORS   Starita,L.M., Pruneda,J.N., Lo,R.S., Fowler,D.M., Kim,H.J.,
            Hiatt,J.B., Shendure,J., Brzovic,P.S., Fields,S. and Klevit,R.E.
  TITLE     Activity-enhancing mutations in an E3 ubiquitin ligase identified
            by high-throughput mutagenesis
  JOURNAL   Proc Natl Acad Sci U S A 110 (14), E1263-E1272 (2013)
   PUBMED   23509263
REFERENCE   7  (bases 1 to 3092)
  AUTHORS   Scaglione,K.M., Zavodszky,E., Todi,S.V., Patury,S., Xu,P.,
            Rodriguez-Lebron,E., Fischer,S., Konen,J., Djarmati,A., Peng,J.,
            Gestwicki,J.E. and Paulson,H.L.
  TITLE     Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP
  JOURNAL   Mol Cell 43 (4), 599-612 (2011)
   PUBMED   21855799
REFERENCE   8  (bases 1 to 3092)
  AUTHORS   Zhang,Y., Zhou,X., Zhao,L., Li,C., Zhu,H., Xu,L., Shan,L., Liao,X.,
            Guo,Z. and Huang,P.
  TITLE     UBE2W interacts with FANCL and regulates the monoubiquitination of
            Fanconi anemia protein FANCD2
  JOURNAL   Mol Cells 31 (2), 113-122 (2011)
   PUBMED   21229326
  REMARK    GeneRIF: UBE2W regulates FANCD2 monoubiquitination by mechanisms
            different from UBE2T and HRR6.
REFERENCE   9  (bases 1 to 3092)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   10 (bases 1 to 3092)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY728312.1, AK082233.1 and AC162448.10.
            
            On Jul 12, 2018 this sequence version replaced NM_025773.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK082233.1, AK046086.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 BY728312.1         48-52
            6-2359              AK082233.1         2-2355
            2360-3092           AC162448.10        27097-27829
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 5.1 cM"
     gene            1..3092
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="ubiquitin-conjugating enzyme E2W (putative)"
                     /db_xref="GeneID:66799"
                     /db_xref="MGI:MGI:1914049"
     exon            1..55
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..496
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable ubiquitin-conjugating enzyme E2 W;
                     ubiquitin-protein ligase W; ubiquitin carrier protein W;
                     N-terminus-conjugating E2; E2 ubiquitin-conjugating enzyme
                     W; N-terminal E2 ubiquitin-conjugating enzyme"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 W isoform 1"
                     /protein_id="NP_080049.3"
                     /db_xref="CCDS:CCDS48224.3"
                     /db_xref="GeneID:66799"
                     /db_xref="MGI:MGI:1914049"
                     /translation="MASMQKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGA
                     PGTLYEGEKFQLLFKFSSRYPFDSPQVMFTGENIPIHPHVYSNGHICLSILTEDWSPA
                     LSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC"
     exon            56..147
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            148..250
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            251..406
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            407..482
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            483..3092
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1186..1191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1208
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     regulatory      1596..1601
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: AATACA"
     polyA_site      1627
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="major polyA site"
     regulatory      1958..1963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: TATAAA"
     polyA_site      1983
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     regulatory      2080..2085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2119
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     polyA_site      2178
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     regulatory      2320..2325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2325
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     polyA_site      2344
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
     regulatory      3076..3081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
                     /note="hexamer: AAGAAA"
     polyA_site      3092
                     /gene="Ube2w"
                     /gene_synonym="6130401J04Rik"
ORIGIN      
        1 gcgtctcggg cctcgctggc cttgggggga tggtttcatc atggcgtcaa tgcagaaacg
       61 actacaaaaa gaactgttgg ctttgcagaa tgacccacct cctggaatga ctttaaatga
      121 aaagagtgtt cagaattcaa tcacgcagtg gatcgtagac atggaaggtg caccaggaac
      181 cttatatgaa ggggaaaaat ttcaactttt gtttaaattt agtagtcgat acccttttga
      241 ctctcctcag gtcatgttta ctggtgaaaa tattcccatt catcctcatg tgtatagcaa
      301 tggtcatatc tgtttatcca ttctaacaga agactggtcc ccggcgctct cagtgcagtc
      361 agtctgtctc agcattatca gcatgctttc cagctgcaaa gaaaagagac gaccaccaga
      421 taattccttt tatgtgcgaa catgtaacaa gaatccaaag aaaacaaaat ggtggtatca
      481 tgatgacact tgttgatgcc gctgccctcc tcctgtagca gaagatagtc ctactgagaa
      541 atgagcactt tgatcattca gtctttgaac tttaaccttt gactggaagt gacctatagg
      601 caatgaagac tacttccttt tactgcattt ttactcgtgt gcattctggg cgcatgttga
      661 tcgctggttc agtccagtca accgacatgc ttttattagt catacagtat taatgcaggt
      721 gtcaggaaat gtcaactata attccatttt ttatttttat ttttttaagc ttttggaaaa
      781 gttccaggtc ctcgtgtatt gtgcaataac agtgacttcc tcagtggttt ggggacgttc
      841 attgccggca atggacgttg taaaaggaag agttttctta actcctgcca cttggtgatt
      901 aatgcatgat agggcctatg aaatgaattt atcagttgtt gtgggattat aaggaaagtg
      961 agggatccaa cattacattg aaagccaccc caattgttta tatttggatt ctatgcactg
     1021 tgatcctaag gccaacagca tgaaattaca tgcatcttta aaggaccgaa agaaagatca
     1081 ttgcatattt atttaattgt ttatatctgc tgtcaaattg ttttgacatg gaaagttttc
     1141 gaggaacatt ggcagagagg tacaatatgt tatccctatg gtgaaaataa attcatttgt
     1201 tgtatatact cctcagtctc tgaagtgaag gtgtgaataa tagagtgtgg atggttaacc
     1261 aaggtgttgt ttggaagtaa aagaaaaaat ggcagtgatg attaaattac tgccatccat
     1321 catttgggcc tgttattatt tttccaagta tattatactc tgttacttcc tgctagccat
     1381 cagcatgagc cctattgcct aaacactatt tcatttattt atgtttggaa aaaatccata
     1441 aacatttttg tttgtaatct tgtttctttt gttatgtttt cagttgttta aatgttataa
     1501 tactttaatt gaaagaattt tgtcaagttt ttttttcttg taaattttct ttacttgtat
     1561 catcttgtcc ttcaatactg taccctaaaa taagaaatac atttttgaca gaggcttaat
     1621 gttttaacaa aagagtgtgg acatttttat tttaagattt tggtaaaagg atactataaa
     1681 aacaatgttt gcttatttgt ctcacttacc cattgtgtag ggtttcttgg aagaacttgt
     1741 tttgtttgtt gagaagattt tgcctacagt tagatgaagt ttttctgtct gcctgtctgt
     1801 ctgtctctac tcttatctat ctgtatgtat gtaggtatct atctatcatc tctctctctg
     1861 atctataaaa caattgttga gaaggttcca tcctaaatac catgtaccat gtaatgatta
     1921 tataactagg tctttttaag aagtgattaa tgtattttat aaattacctt ttcacatatg
     1981 caagctctgt ttctatgaca atgttatttt tactgatgcc ttactgttat agtctttttg
     2041 aatatcctgc agtgaatata ttaatcaatt tgggctaaaa ataaaagcca gttggctgaa
     2101 aggtctgacg gtatgtaaac ccataaaaaa atcattcaac tgataattgg taaagaatat
     2161 tttaaaaatt gtttttaacc tctgtgtaga tgacattttg tagcttttta catgttgtta
     2221 attaagggca tataatttta cactctaaaa gtataactgc tgaactcagg ggtgggtaga
     2281 cttcaaaaat atgtctactg tagaaataac ttgaaaaaaa ttaaatccat ggccagccac
     2341 caaattgtgg acactgttta taaaactagg gagcaatcat cattttttaa agattataaa
     2401 cgttttcttc tgtcttgact gcatgtatat aagttcccac agtaaccatt atcagtttta
     2461 ttagttggcc ttcttgacat gaaaaaaatc tttacgttct tacgtttctt ctgcttttac
     2521 gatgaaatca cagctgtaag ttaagtttat gtcactgctc taaatattaa ttagctcttt
     2581 tgtcgggttg gggacacagg gagaaacact tacctatccg tggtcctctt actaaaatcc
     2641 tggcctgtga cgtcactcac cgaaggagct cccacaccag gtcagcagca acagtgctgg
     2701 aatgtagatc accagcttcc gttgtgcttt tataacagtg ctttactata cctctctcta
     2761 tgcagacact gtagggcaga cgcaaatgaa atgggaaata gggaaaaacc aaacactttt
     2821 gcttcatgtt tggtaccaag agaagaaatt aaaaatatat ctaaaatagt ggagcatcaa
     2881 ctattactaa ttcataatta gattaatatt actcctttga atttagaagc cactgaaaat
     2941 tcttttacac ttgcatttaa ttatttgtca ttattaagag tcataagatg tgtatctggg
     3001 tcattgttct gttgagggca agttataaaa caaatatttt gctgtaatgt taactactga
     3061 aagagccaga attacaagaa atgcatatga aa
//
