LOCUS       NM_023230               1950 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2 variant 1 (Ube2v1),
            transcript variant 2, mRNA.
ACCESSION   NM_023230
VERSION     NM_023230.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Zhao,T., Tang,X., Umeshappa,C.S., Ma,H., Gao,H., Deng,Y.,
            Freywald,A. and Xiang,J.
  TITLE     Simulated Microgravity Promotes Cell Apoptosis Through Suppressing
            Uev1A/TICAM/TRAF/NF-kappaB-Regulated Anti-Apoptosis and p53/PCNA-
            and ATM/ATR-Chk1/2-Controlled DNA-Damage Response Pathways
  JOURNAL   J Cell Biochem 117 (9), 2138-2148 (2016)
   PUBMED   26887372
  REMARK    GeneRIF: work reveals that simulated microgravity promotes the
            apoptotic response through a combined modulation of the
            Uev1A/TICAM/TRAF/NF-kappaB-regulated apoptosis and the p53/PCNA-
            and ATM/ATR-Chk1/2-controlled DNA-damage response pathways.
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Mural,R.V., Liu,Y., Rosebrock,T.R., Brady,J.J., Hamera,S.,
            Connor,R.A., Martin,G.B. and Zeng,L.
  TITLE     The tomato Fni3 lysine-63-specific ubiquitin-conjugating enzyme and
            suv ubiquitin E2 variant positively regulate plant immunity
  JOURNAL   Plant Cell 25 (9), 3615-3631 (2013)
   PUBMED   24076975
REFERENCE   3  (bases 1 to 1950)
  AUTHORS   Dauphinee,S.M., Clayton,A., Hussainkhel,A., Yang,C., Park,Y.J.,
            Fuller,M.E., Blonder,J., Veenstra,T.D. and Karsan,A.
  TITLE     SASH1 is a scaffold molecule in endothelial TLR4 signaling
  JOURNAL   J Immunol 191 (2), 892-901 (2013)
   PUBMED   23776175
REFERENCE   4  (bases 1 to 1950)
  AUTHORS   Hoxhaj,G., Najafov,A., Toth,R., Campbell,D.G., Prescott,A.R. and
            MacKintosh,C.
  TITLE     ZNRF2 is released from membranes by growth factors and, together
            with ZNRF1, regulates the Na+/K+ATPase
  JOURNAL   J Cell Sci 125 (Pt 19), 4662-4675 (2012)
   PUBMED   22797923
  REMARK    Erratum:[J Cell Sci. 2022 Mar 15;135(6):jcs259936. doi:
            10.1242/jcs.259936.. PMID: 35267011]
REFERENCE   5  (bases 1 to 1950)
  AUTHORS   Wu,J., Chen,Y., Lu,L.Y., Wu,Y., Paulsen,M.T., Ljungman,M.,
            Ferguson,D.O. and Yu,X.
  TITLE     Chfr and RNF8 synergistically regulate ATM activation
  JOURNAL   Nat Struct Mol Biol 18 (7), 761-768 (2011)
   PUBMED   21706008
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1950)
  AUTHORS   Petroski,M.D., Zhou,X., Dong,G., Daniel-Issakani,S., Payan,D.G. and
            Huang,J.
  TITLE     Substrate modification with lysine 63-linked ubiquitin chains
            through the UBC13-UEV1A ubiquitin-conjugating enzyme
  JOURNAL   J Biol Chem 282 (41), 29936-29945 (2007)
   PUBMED   17709375
  REMARK    GeneRIF: modification of proteins with Lys(63)-linked ubiquitin
            chains occurs through a UEV1A-independent substrate modification
            and UEV1A-dependent Lys(63)-linked ubiquitin chain synthesis
            mechanism
REFERENCE   7  (bases 1 to 1950)
  AUTHORS   Ashley,C., Pastushok,L., McKenna,S., Ellison,M.J. and Xiao,W.
  TITLE     Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked
            ubiquitination
  JOURNAL   Gene 285 (1-2), 183-191 (2002)
   PUBMED   12039045
REFERENCE   8  (bases 1 to 1950)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 1950)
  AUTHORS   Franko,J., Ashley,C. and Xiao,W.
  TITLE     Molecular cloning and functional characterization of two murine
            cDNAs which encode Ubc variants involved in DNA repair and
            mutagenesis
  JOURNAL   Biochim Biophys Acta 1519 (1-2), 70-77 (2001)
   PUBMED   11406273
REFERENCE   10 (bases 1 to 1950)
  AUTHORS   JACKSON,B.R. and DANIEL,W.H.
  TITLE     Recurrent carcinoma of the rectum, surgical treatment
  JOURNAL   Calif Med 91 (3), 149-150 (1959)
   PUBMED   14406273
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK158230.1.
            
            On Sep 14, 2006 this sequence version replaced NM_023230.1.
            
            Transcript Variant: This variant (2) lacks an internal exon which
            results in the use of an alternate start codon compared to variant
            1. The encoded isoform (2) is shorter and has a distinct N-terminus
            compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158230.1, SRR1660817.57555.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.44 cM"
     gene            1..1950
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /note="ubiquitin-conjugating enzyme E2 variant 1"
                     /db_xref="GeneID:66589"
                     /db_xref="MGI:MGI:1913839"
     exon            1..66
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..488
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 variant 1
                     isoform 2"
                     /protein_id="NP_075719.1"
                     /db_xref="CCDS:CCDS17103.1"
                     /db_xref="GeneID:66589"
                     /db_xref="MGI:MGI:1913839"
                     /translation="MAATTGSGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMT
                     LTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPSVRFVTRVNMSGVSSSNGVVDP
                     RATAVLAKWQNSHSIKVILQELRRLMMSKENMKLPQPPEGQCYSN"
     exon            67..215
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /inference="alignment:Splign:2.1.0"
     exon            216..341
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /inference="alignment:Splign:2.1.0"
     exon            342..1950
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1719..1724
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /note="hexamer: AATAAA"
     polyA_site      1746
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
                     /note="major polyA site"
     polyA_site      1765
                     /gene="Ube2v1"
                     /gene_synonym="0610011J09Rik; CROC-1; CROC1; D7Bwg1382e;
                     UEV-1"
ORIGIN      
        1 ggggggtgaa gaaggggccg gccttcgagc gacagcgacg caagatggca gccaccacag
       61 gctcgggagt aaaagtccct cgaaatttcc gactgttgga agagctggaa gaaggacaga
      121 aaggagtagg cgacggcaca gttagctggg gtctggagga cgacgaggac atgacactta
      181 caagatggac aggcatgata attggacctc cacgaacaat ctatgaaaac cgaatataca
      241 gccttaagat agagtgtggg cctaagtacc cagaggcacc cccgtctgta agattcgtaa
      301 caagagtcaa tatgagcggc gtgagcagtt cgaatggagt ggtggatccg agagccacgg
      361 cagtgctggc aaagtggcag aactcccaca gcatcaaagt catcctgcag gaactgcggc
      421 gcctgatgat gtcaaaagag aacatgaagc tgccacagcc gccggaagga cagtgttaca
      481 gcaattagtc accaaggccc cggcctcccc tccccatcca acccaagtct tcattttcca
      541 cagtagtgaa ttttctagat acatctgtag acctcaaagt accggagagg aagctccact
      601 cagccttcat cctgagacac tgttctgata ctaacttctc gtccatttga aatacctaag
      661 ttgtgctgtg taacatcaac tgttgagtgt aaccgctgtc tgcctggttg aacttctggg
      721 atcaagaagg tgttgaaatc ggtttcctgt gggagcggtg ggcacagcta acgcaactgt
      781 gaacagacac gtcacacaat cacctgctgc tggcccttgg cctgggtctg cctttgcccg
      841 ccccgccctc cgccacagct gtgtggtggc ccttagaata gatgggaagg cttcaggtag
      901 cagttgtggg actgactact gctgggcttg gggcgctttg gctgcacccc tgcttcttaa
      961 gtcttaagtg atgccccatc caagccatgg tccccactcc tccactccca cccttggcca
     1021 aagcttagat tgtaaccctc ccctccctct gaaattggcc gcgggtgagg aattcagggc
     1081 ttcccgtgtc cccaccttta tcaaggggtg ctgcttcccc tcctcaactc ccctgttgcc
     1141 catcaccacc caacacttgc tgtggccaga agccatcaga tgaggttgga attgcctggc
     1201 ctccctcact tagctccgga ccacactcac ctgccaccag cctgggaagg gagcgccggg
     1261 tcctcagccc tgctgccacc gtcagccctc gagctttgtg ctcaggtcta aggtaaacag
     1321 cagtcacagg gcgactcgga ccggccagcg ctcagggtcc ttggtgaaaa gatgcctaga
     1381 gaaaggccat gcggagtcct cagtgcacac ggttctgagt tggttccttt tcccatcatt
     1441 gctttgcttt tacacattga agaggtttga acagggctct cgtcaccctg gccacccatc
     1501 gggtctgatg tggagcctga cagctggagc acgaggccct gaccctgcag actcaggttt
     1561 cagtgttgct cctcagaccc tctgcaggga ccaggaggag ctctgtgtcc ccatgtttgc
     1621 agatcatctg cagatccgtg ggcctggcct cctggctcac tgccacgctc cccactctgt
     1681 tcaggaccac taaatgttga aatgtggatg cataccgaaa taaaggcaat ccgttgtgtt
     1741 gcagagctcg gtggttttat tttaagcacg tgtgtggctc caccttctga agcagcagct
     1801 atgaggtcta aagagtggct ggtggggcct ttgatctatc tggggcaaac tagctctccg
     1861 gtaggcaccc tgtaagtcaa gcttgactgt ccataagcaa cgttctgtcg tcgtccgagt
     1921 ctactataat aaaaaagtat gctttgatac
//
