LOCUS       NM_080560               3851 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2N (Ube2n), mRNA.
ACCESSION   NM_080560
VERSION     NM_080560.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Zhang,C., Jia,Q., Zhu,L., Hou,J., Wang,X., Li,D., Zhang,J.,
            Zhang,Y., Yang,S., Tu,Z., Yan,X.X., Yang,W., Li,S., Li,X.J. and
            Yin,P.
  TITLE     Suppressing UBE2N ameliorates Alzheimer's disease pathology through
            the clearance of amyloid beta
  JOURNAL   Alzheimers Dement 20 (9), 6287-6304 (2024)
   PUBMED   39015037
  REMARK    GeneRIF: Suppressing UBE2N ameliorates Alzheimer's disease
            pathology through the clearance of amyloid beta.
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3851)
  AUTHORS   Li,Z., Li,G., Li,Y., Luo,Y., Jiang,Y., Zhang,Z., Zhou,Z., Liu,S.,
            Wu,C. and You,F.
  TITLE     Deubiquitinase OTUD6A Regulates Innate Immune Response via
            Targeting UBC13
  JOURNAL   Viruses 15 (8), 1761 (2023)
   PUBMED   37632103
  REMARK    GeneRIF: Deubiquitinase OTUD6A Regulates Innate Immune Response via
            Targeting UBC13.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3851)
  AUTHORS   Babcock,R.L., Zhou,Y., Patel,B., Chrisikos,T.T., Kahn,L.M.,
            Dyevoich,A.M., Medik,Y.B. and Watowich,S.S.
  TITLE     Regulation and function of Id2 in plasmacytoid dendritic cells
  JOURNAL   Mol Immunol 148, 6-17 (2022)
   PUBMED   35640521
REFERENCE   5  (bases 1 to 3851)
  AUTHORS   Kumari,R., Kumar,R., Dey,A.K., Saha,S. and Maiti,T.K.
  TITLE     S-Nitrosylation of OTUB1 Alters Its Stability and Ubc13 Binding
  JOURNAL   ACS Chem Neurosci 13 (10), 1517-1525 (2022)
   PUBMED   35500217
  REMARK    GeneRIF: S-Nitrosylation of OTUB1 Alters Its Stability and Ubc13
            Binding.
REFERENCE   6  (bases 1 to 3851)
  AUTHORS   Zhou,H., Wertz,I., O'Rourke,K., Ultsch,M., Seshagiri,S., Eby,M.,
            Xiao,W. and Dixit,V.M.
  TITLE     Bcl10 activates the NF-kappaB pathway through ubiquitination of
            NEMO
  JOURNAL   Nature 427 (6970), 167-171 (2004)
   PUBMED   14695475
REFERENCE   7  (bases 1 to 3851)
  AUTHORS   Ashley,C., Pastushok,L., McKenna,S., Ellison,M.J. and Xiao,W.
  TITLE     Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked
            ubiquitination
  JOURNAL   Gene 285 (1-2), 183-191 (2002)
   PUBMED   12039045
REFERENCE   8  (bases 1 to 3851)
  AUTHORS   Franko,J., Ashley,C. and Xiao,W.
  TITLE     Molecular cloning and functional characterization of two murine
            cDNAs which encode Ubc variants involved in DNA repair and
            mutagenesis
  JOURNAL   Biochim Biophys Acta 1519 (1-2), 70-77 (2001)
   PUBMED   11406273
REFERENCE   9  (bases 1 to 3851)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 3851)
  AUTHORS   JACKSON,B.R. and DANIEL,W.H.
  TITLE     Recurrent carcinoma of the rectum, surgical treatment
  JOURNAL   Calif Med 91 (3), 149-150 (1959)
   PUBMED   14406273
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159135.11.
            
            On Nov 17, 2006 this sequence version replaced NM_080560.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067069.1, SRR9219381.107725.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AC159135.11        35691-35766         c
            77-323              AC159135.11        9477-9723           c
            324-464             AC159135.11        9150-9290           c
            465-3851            AC159135.11        5270-8656           c
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 49.41 cM"
     gene            1..3851
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /note="ubiquitin-conjugating enzyme E2N"
                     /db_xref="GeneID:93765"
                     /db_xref="MGI:MGI:1934835"
     exon            1..76
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..505
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-protein ligase N; ubiquitin carrier
                     protein N; bendless-like ubiquitin-conjugating enzyme; E2
                     ubiquitin-conjugating enzyme N"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 N"
                     /protein_id="NP_542127.1"
                     /db_xref="CCDS:CCDS48677.1"
                     /db_xref="GeneID:93765"
                     /db_xref="MGI:MGI:1934835"
                     /translation="MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQD
                     SPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIR
                     TVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI"
     exon            77..323
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /inference="alignment:Splign:2.1.0"
     exon            324..464
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /inference="alignment:Splign:2.1.0"
     exon            465..3851
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1458..1463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /note="hexamer: AATAAA"
     polyA_site      1478
                     /gene="Ube2n"
                     /gene_synonym="1500026J17Rik; UBC13"
                     /note="major polyA site"
ORIGIN      
        1 gcggagccgg agacaagagc agaggccgaa ctcgggatct gacaagatgg ccgggctgcc
       61 ccgcaggatc atcaaggaaa cccagcgttt gctggcagaa ccagttcctg gcattaaagc
      121 agaaccagat gagagcaacg cccgttattt tcatgtggtc attgctggcc cccaggattc
      181 cccctttgag ggagggactt ttaaacttga actattcctt ccagaagaat acccaatggc
      241 agcacctaaa gtacgtttca tgaccaaaat ttatcatcct aatgtagaca agttgggaag
      301 aatatgttta gatattttga aagataagtg gtccccagca ctgcagatcc gcacagttct
      361 gctatcaatc caggctttgt taagtgctcc taatccagat gatccattag caaatgatgt
      421 agccgagcaa tggaagacca acgaagccca agccatagaa acagcgagag catggactag
      481 gctatatgcc atgaacaata tttaagtcga tcagatcatc gagtgtacac cacttctcct
      541 gttctgccaa gacttcttcc tttttttgtt tgcatttaat ggacacagtc ttagaaacat
      601 tacagaataa aagcccaggc atcttcatct ttggtgatta catgcacatt agcaaatctg
      661 tcttgtcctg attcactgtt gtaaagcatg agcagaggct agaagtacca tccgggttgc
      721 tgagagatgc tgagaagcag gactctctgc tttgactcct tccccatcat ggttaaagta
      781 caaggcactg tgaatgaagg tagttgtcag ggttagctgc caggggtatg ggtgttattt
      841 tatttttagg gggaaagtag ttttatttta attttatggc ccccttcccc ctcccccctc
      901 ttaaaggatc taattgcagt ggttaaatgc agctaaccag tttttttaga atatgctctc
      961 tagccaagtc taactttaga cgctgtagat ggacaagctt gattgtctga accaaaatgg
     1021 gaacattaaa taaacatcac agccctcact aataacattg tgactttgct gtcaagtgta
     1081 gattctccac ccccaccccc accccgaaaa gacgaagaaa aagcttgtga ccattttgta
     1141 tggcttgtct ggaaacttct gtaaatctta tgttttagta aaatattttt tgttattcta
     1201 ctttgccttt gtacagttta ttttactgtg tttatttcgc tttcccgatg tgacaatcgt
     1261 atttaaaatt aaaatggatg ggacattctg ttttagtcct ctccatctga caaattggca
     1321 agaggagaat tttaccagtt tgtttcattg atgcagatct gtgttaggat tctactgagt
     1381 gagtatttat aaactggctc tgagcatgca tgaagcatca atatctcttt tttttcttaa
     1441 gcctcctaga aatttgaaat aaatattacc tgattcaatt aggtgatcct tggtgtcctt
     1501 gccatgtttg aaagttgacc atactcagca gtcacagtga agagggcagt tgcatgtcac
     1561 atatggcatt tgagcttcag gtgtaggtta tgacttctct tggttcttgt ctgaacacta
     1621 tatgaccatt ggtttgtgct attaggaaaa ctctagttat ttgattttgc tgaggcagtt
     1681 ttgagtcctc ctgcagacct aatgaacccg gcaagtggtg tcattggctc ttcagttcct
     1741 gtgcatttgc tatgtagccc tggctgtcct ggaactcact attacagacc aggctggcct
     1801 tcaacccaga gatctggctg cctcttctag gatcaaaggc cactgtgccc agctattcct
     1861 tttatccttt aatttgctct tagtagttgt tttttttcct gtgtaaaaca atccctttcc
     1921 acattttcta catggaattt ctgattcttg ctattttaaa gtcgtataca tgtaaatgct
     1981 aaccttatgg tcagaaatct tcatctccat aatcttcatg caaggttatg gatttcttca
     2041 taacttaagt tttttttctc ttttgaatgc agactatttt attgtgttag aatagaattt
     2101 aatagaatta tctattaaaa ttatattttc aacatataag agcttttgac aactgataac
     2161 tggatccttc caaataaatg cattttgtta acaaatgtaa agtttagaat aaaattgcta
     2221 atggccctac ctgatactgt gaaattttaa actctctaac agcagttgtt acaattcttg
     2281 gcctgagtac atctttgtcc atggctaaga cagttctcac tttaaaaaag cagacttgac
     2341 aggacttgaa gttaaccatt caccactcag cattccatcc tgaaagccag agctgctcct
     2401 agtcatagct gatgccttac acattaccca cagtggcctg gtgggcagag gtttcagttt
     2461 tcccagttta cattaggact cctaatttcc tggcatcaca ttttctttga aggttttgat
     2521 gagtcccatt gtttgaaatt tgctctctta gccagttgga agaagcaaga attgttactt
     2581 atgcctgggc tgaaaagaaa caacaggttg aggtggaggt tgaactctgg taactacacc
     2641 tggcttatgg acctgttttc gttggccagg aaaatggttg gttcagagct gttaaatgat
     2701 agtattgcag aaactcggga tagtgaataa gacgtagcac taattagact gatgatcatt
     2761 gtgtggacat ctcctggcag gagctgagta gcagttacca ctggatgaca tgccattctc
     2821 tttttctgtt taaagtaggt cggaatcatt cccagcctac ctgtttctgt atgtgtggtt
     2881 ctgttttctt acaaagtttt gactttgtag ccccagcaaa agaaagacat tcacttaaca
     2941 aaagcatatt aacatttgcc tgctgcttgc tggtttgagg atgtagatgt gaatgaatag
     3001 tttgggcctt tcctttgaag ggacagcata ggcgtgggaa caacacaata gttgcagctc
     3061 ctcttggaaa ggctaatctt ctggactcaa atcagacaaa cgcctccaac ctccagaaat
     3121 ctgcacatgc agagctcaga gagagaagaa aaccaacttc ttccaaattg gcatcttgaa
     3181 gtcagttctg aagccctaga gtataaatgt gggctttgtg gtttgaagtt tttgttctct
     3241 tttgttgtct attttgctgt gattcacttc tactgtgaga ttagttagcc ttggttcact
     3301 cagatcatag gcattctgta tataatactg aatactcaca ctagaccagg aatgtacttg
     3361 taagataaag ttcagtgtaa atcgatatat tcaaaccaca ggggtgctgt gctcagcatt
     3421 tctccggagg gtggggaaga ccatctggtg ttctgtaaga gtgctcccag tcagccaagt
     3481 agacctcagt ggtgtcatgt ggacagttgg ttggctttgt ttccctggtg gtttaggtat
     3541 tgttctcttg actaatgtaa taacatcagt ttttattact gcagaatcgg aagttttagg
     3601 actacatagg agagctatcc acatacaaat tacgaagtga gatgagaaat tgggtaccta
     3661 acccactatt ccgtaagaaa tatgtaatat ttcattggaa tgtattctgg ggctgggagc
     3721 aacatttcat ttagctatga agcatgccgc cttttaagga gctatatcag ggtttatatt
     3781 ttaagtaaaa ggaaaacatg ttgaatgtaa tttcaaagaa gtataaacaa taaaaacgta
     3841 taccgattgg a
//
