LOCUS       NM_145578               1295 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript
            variant 1, mRNA.
ACCESSION   NM_145578
VERSION     NM_145578.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Lu,X., Kong,X., Wu,H., Hao,J., Li,S., Gu,Z., Zeng,X., Shen,Y.,
            Wang,S., Chen,J., Fei,X., Sun,Y., Li,X., Jiang,L., Yang,F., Wang,J.
            and Cai,Z.
  TITLE     UBE2M-mediated neddylation of TRIM21 regulates obesity-induced
            inflammation and metabolic disorders
  JOURNAL   Cell Metab 35 (8), 1390-1405 (2023)
   PUBMED   37343564
  REMARK    GeneRIF: UBE2M-mediated neddylation of TRIM21 regulates
            obesity-induced inflammation and metabolic disorders.
REFERENCE   3  (bases 1 to 1295)
  AUTHORS   Wu,D., Li,H., Liu,M., Qin,J. and Sun,Y.
  TITLE     The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are
            essential for the maintenance of Regulatory T cell fitness
  JOURNAL   Nat Commun 13 (1), 3021 (2022)
   PUBMED   35641500
  REMARK    GeneRIF: The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are
            essential for the maintenance of Regulatory T cell fitness.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1295)
  AUTHORS   Zou,J., Ma,W., Li,J., Littlejohn,R., Zhou,H., Kim,I.M.,
            Fulton,D.J.R., Chen,W., Weintraub,N.L., Zhou,J. and Su,H.
  TITLE     Neddylation mediates ventricular chamber maturation through
            repression of Hippo signaling
  JOURNAL   Proc Natl Acad Sci U S A 115 (17), E4101-E4110 (2018)
   PUBMED   29632206
REFERENCE   5  (bases 1 to 1295)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1295)
  AUTHORS   Werber,M., Wittler,L., Timmermann,B., Grote,P. and Herrmann,B.G.
  TITLE     The tissue-specific transcriptomic landscape of the mid-gestational
            mouse embryo
  JOURNAL   Development 141 (11), 2325-2330 (2014)
   PUBMED   24803591
REFERENCE   7  (bases 1 to 1295)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 1295)
  AUTHORS   Pawlowski,T.L., Heringer-Walther,S., Cheng,C.H., Archie,J.G.,
            Chen,C.F., Walther,T. and Srivastava,A.K.
  TITLE     Candidate Agtr2 influenced genes and pathways identified by
            expression profiling in the developing brain of Agtr2(-/y) mice
  JOURNAL   Genomics 94 (3), 188-195 (2009)
   PUBMED   19501643
REFERENCE   9  (bases 1 to 1295)
  AUTHORS   Huang,D.T., Ayrault,O., Hunt,H.W., Taherbhoy,A.M., Duda,D.M.,
            Scott,D.C., Borg,L.A., Neale,G., Murray,P.J., Roussel,M.F. and
            Schulman,B.A.
  TITLE     E2-RING expansion of the NEDD8 cascade confers specificity to
            cullin modification
  JOURNAL   Mol Cell 33 (4), 483-495 (2009)
   PUBMED   19250909
REFERENCE   10 (bases 1 to 1295)
  AUTHORS   Kim,A.Y., Bommelje,C.C., Lee,B.E., Yonekawa,Y., Choi,L.,
            Morris,L.G., Huang,G., Kaufman,A., Ryan,R.J., Hao,B., Ramanathan,Y.
            and Singh,B.
  TITLE     SCCRO (DCUN1D1) is an essential component of the E3 complex for
            neddylation
  JOURNAL   J Biol Chem 283 (48), 33211-33220 (2008)
   PUBMED   18826954
  REMARK    GeneRIF: SCCRO recruits Ubc12 approximately NEDD8 to the
            CAND1-Cul1-ROC1 complex but that this is not sufficient to
            dissociate or overcome the inhibitory effects of CAND1 on cullin
            neddylation
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121301.8.
            
            On Sep 6, 2011 this sequence version replaced NM_145578.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021792.1, BQ899397.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               AC121301.8         113151-113508       c
            359-453             AC121301.8         112096-112190       c
            454-492             AC121301.8         111964-112002       c
            493-596             AC121301.8         111742-111845       c
            597-660             AC121301.8         111372-111435       c
            661-1295            AC121301.8         110650-111284       c
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 7.73 cM"
     gene            1..1295
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /note="ubiquitin-conjugating enzyme E2M"
                     /db_xref="GeneID:22192"
                     /db_xref="MGI:MGI:108278"
     exon            1..358
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..801
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /EC_number="2.3.2.34"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin C, related sequence 2; UBC12 homolog, yeast;
                     NEDD8-conjugating enzyme Ubc12; NEDD8 protein ligase;
                     NEDD8 carrier protein"
                     /codon_start=1
                     /product="NEDD8-conjugating enzyme Ubc12 isoform 1"
                     /protein_id="NP_663553.1"
                     /db_xref="CCDS:CCDS20825.1"
                     /db_xref="GeneID:22192"
                     /db_xref="MGI:MGI:108278"
                     /translation="MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLP
                     KTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHP
                     NIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLF
                     EQNVQRSMRGGYIGSTYFERCLK"
     exon            359..453
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            454..492
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            493..596
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            597..660
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            661..1295
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1156..1161
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /note="hexamer: ATTAAA"
     polyA_site      1177
                     /gene="Ube2m"
                     /gene_synonym="2510040H03Rik; Ubc-rs2; UBC12"
                     /note="major polyA site"
ORIGIN      
        1 ccgaggatcg ccgagtcctc ccgagcacct ccgaacacac ccgaggtgct tagccaggcc
       61 agagccggac tacgggagcc gaggcgggcc gtgcggtggg cgcggagcag cgcggcaggc
      121 cgggcgagcg gcagcggagg aggccgcggt agcgggtccg aggagcggaa gcggcgcagg
      181 cggcggcggg gggccgggtg gccggggtcc cgggcccccg cggcggcggg ctgcggcggc
      241 ggcggcagga tgatcaagct gttctcgctg aagcagcaga agaaggagga ggagtcggcc
      301 ggcggcacca agggcagcag caagaaggcg tcggcggcgc agctccggat tcagaaggac
      361 attaacgagc tgaacctgcc caagacgtgt gacatcagct tctcagaccc agacgacctc
      421 ctcaacttca agctggtgat ctgtcctgat gaaggcttct acaaaagtgg caagtttgta
      481 ttcagcttta aggtgggaca gggttaccca catgaccctc ccaaggtgaa gtgtgaaaca
      541 atggtttatc atcccaacat tgacctcgag ggcaacgtct gcctcaacat cctcagagag
      601 gactggaagc cagtccttac gataaactcc ataatttatg gcctgcagta tctcttcttg
      661 gagccgaacc ctgaggaccc actgaacaag gaggctgctg aggtcctgca gaacaaccgg
      721 cggctgtttg aacaaaatgt gcagcgctcc atgagaggtg gttacatcgg gtccacctac
      781 ttcgagcgct gcctgaaata gggttagcaa ttacccatcc ctgccagggt taccggccca
      841 ggcatcccct gcaaatattt attgggggcc cgggtaaggt gtgtggggta gccttggccc
      901 ctgccttggc cttgcctctc ttccctgtca cctgccccta gttatttttt ttttgaccac
      961 cacgtgatta tggtcggtgc tgcctccccc ccgacctgct cagcgatggg aaatgaattg
     1021 gcttgtttag cgcccccccc ctcccgctgg gtgctgtcca actccccact cttgactgtg
     1081 gggtaagtgg gcaatgggcc tgggtcgcta ggcccaagca acccacccca ccaccactgg
     1141 aggtcccacc aggctattaa aggggaatgt tactgcatgg cctctgcctc ttccttctgt
     1201 ggggtgggag gtacaagtga ggtccaccag cgtctataat gaagataaat gtggccgtgg
     1261 attgggtggg accactcact gggatgaggt gcaag
//
