LOCUS       NM_016786               4882 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2K (Ube2k), transcript
            variant 1, mRNA.
ACCESSION   NM_016786
VERSION     NM_016786.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Fu,W., Lei,X., Lu,Q., Zhang,J., Guo,J., Zhao,J., Tong,X. and Hu,X.
  TITLE     UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness
            in pancreatic ductal adenocarcinoma
  JOURNAL   Int J Oncol 62 (4) (2023)
   PUBMED   36896783
  REMARK    GeneRIF: UBE2K regulated by IGF2BP3 promotes cell proliferation and
            stemness in pancreatic ductal adenocarcinoma.
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Kelam,N., Racetin,A., Katsuyama,Y., Vukojevic,K. and Kostic,S.
  TITLE     Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and
            HIP2 in Developing and Postnatal Kidneys of Dab1-/- (yotari) Mice
  JOURNAL   Int J Mol Sci 23 (4), 2025 (2022)
   PUBMED   35216141
  REMARK    GeneRIF: Immunohistochemical Expression Pattern of FGFR1, FGFR2,
            RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-)
            (yotari) Mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4882)
  AUTHORS   Su,J., Huang,P., Qin,M., Lu,Q., Sang,X., Cai,Y., Wang,Y., Liu,F.,
            Wu,R., Wang,X., Jiang,X., Wang,J., Sun,Q., Chen,S. and Xu,J.
  TITLE     Reduction of HIP2 expression causes motor function impairment and
            increased vulnerability to dopaminergic degeneration in Parkinson's
            disease models
  JOURNAL   Cell Death Dis 9 (10), 1020 (2018)
   PUBMED   30282965
  REMARK    GeneRIF: HIP2 insufficiency as a contributing factor for PD.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4882)
  AUTHORS   Jeong,E.I., Chung,H.W., Lee,W.J., Kim,S.H., Kim,H., Choi,S.G. and
            Jung,Y.K.
  TITLE     E2-25K SUMOylation inhibits proteasome for cell death during
            cerebral ischemia/reperfusion
  JOURNAL   Cell Death Dis 7 (12), e2573 (2016)
   PUBMED   28032866
  REMARK    GeneRIF: E2-25K has a crucial role in oxidative stress and cerebral
            Ischemia/Reperfusion-induced damage through inhibiting proteasome
            via its SUMOylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4882)
  AUTHORS   Rock,J.R. and Harfe,B.D.
  TITLE     Expression of TMEM16 paralogs during murine embryogenesis
  JOURNAL   Dev Dyn 237 (9), 2566-2574 (2008)
   PUBMED   18729231
REFERENCE   6  (bases 1 to 4882)
  AUTHORS   Song,S., Lee,H., Kam,T.I., Tai,M.L., Lee,J.Y., Noh,J.Y., Shim,S.M.,
            Seo,S.J., Kong,Y.Y., Nakagawa,T., Chung,C.W., Choi,D.Y.,
            Oubrahim,H. and Jung,Y.K.
  TITLE     E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta
            neurotoxicity
  JOURNAL   J Cell Biol 182 (4), 675-684 (2008)
   PUBMED   18710920
  REMARK    GeneRIF: E2-25K/Hip-2 is thus an essential upstream regulator of
            the expression and activation of caspase-12 in endoplasmic
            reticulum stress-mediated Abeta neurotoxicity.
REFERENCE   7  (bases 1 to 4882)
  AUTHORS   Wassler,M.J., Shur,B.D., Zhou,W. and Geng,Y.J.
  TITLE     Characterization of a novel ubiquitin-conjugating enzyme that
            regulates beta1,4-galactosyltransferase-1 in embryonic stem cells
  JOURNAL   Stem Cells 26 (8), 2006-2018 (2008)
   PUBMED   18511602
REFERENCE   8  (bases 1 to 4882)
  AUTHORS   Wang,Y., Saigoh,K., Osaka,H., Yamanishi,T., Suh,J., Kiyosawa,H.,
            Sakai,Y., Wakana,S. and Wada,K.
  TITLE     YAC/BAC-based physical and transcript mapping around the gracile
            axonal dystrophy (gad) locus identifies Uchl1, Pmx2b, Atp3a2, and
            Hip2 genes
  JOURNAL   Genomics 66 (3), 333-336 (2000)
   PUBMED   10873389
REFERENCE   9  (bases 1 to 4882)
  AUTHORS   Tanno,Y., Mori,T., Yokoya,S., Kanazawa,K., Honma,Y., Nikaido,T.,
            Takeda,J., Tojo,M., Yamamoto,T. and Wanaka,A.
  TITLE     Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in
            the developing mouse brain
  JOURNAL   J Chem Neuroanat 17 (2), 99-107 (1999)
   PUBMED   10585161
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK139564.1, AK036068.1 and AK149463.1.
            
            On Jul 16, 2015 this sequence version replaced NM_016786.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.69688.1, AK048194.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AK139564.1         2-157
            157-2135            AK036068.1         82-2060
            2136-3969           AK149463.1         46-1879
            3970-4882           AK149463.1         1881-2793
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 33.72 cM"
     gene            1..4882
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /note="ubiquitin-conjugating enzyme E2K"
                     /db_xref="GeneID:53323"
                     /db_xref="MGI:MGI:1858216"
     exon            1..302
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     CDS             240..842
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-protein ligase; ubiquitin carrier protein;
                     ubiquitin-conjugating enzyme E2-25 kDa;
                     ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast);
                     huntingtin interacting protein 2; E2 ubiquitin-conjugating
                     enzyme K"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 K isoform 1"
                     /protein_id="NP_058066.2"
                     /db_xref="CCDS:CCDS39098.1"
                     /db_xref="GeneID:53323"
                     /db_xref="MGI:MGI:1858216"
                     /translation="MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIA
                     GPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAA
                     AMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSP
                     EYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN"
     exon            303..396
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     exon            397..455
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     exon            456..538
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     exon            539..638
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     exon            639..767
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     exon            768..4882
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1013..1018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /note="hexamer: ATTAAA"
     polyA_site      1038
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /note="major polyA site"
     regulatory      1617..1622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /note="hexamer: AATAAA"
     polyA_site      1648
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
     regulatory      4803..4808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
                     /note="hexamer: AATAAA"
     polyA_site      4822
                     /gene="Ube2k"
                     /gene_synonym="D5Ertd601e; E2-25k; HIP-2; Hip2; Hypg; Lig"
ORIGIN      
        1 gccgcccggt cgggtcagcg tggtgcgcat gcgcggaccc ggcgccattt tggtggccgg
       61 gcgcggaggt gattctacag tgaggaggaa cgcgacggcg agggtggcag tggcagcgtt
      121 cgtgtgctcg ggtctgaatc gctgagggag gaggcggcgg cggaggaaga ggtggcagcg
      181 gtggcggtgg tcgtagcggt ggcggaggag gcgggtacga atcagctgcg ggcagagaca
      241 tggccaacat cgcggtgcag cgaatcaagc gggagttcaa ggaggtgctg aagagcgagg
      301 agacgagcaa aaatcaaatt aaagtagatc ttgtagatga gaattttaca gaattaagag
      361 gagaaatagc aggacctcca gacacaccgt atgaaggtgg aagatatcaa ctagagataa
      421 aaataccaga aacatatcca tttaaccccc ctaaggtccg gtttatcact aaaatatggc
      481 accctaatat tagttccgtc acaggggcta tttgtttgga tatcctgaaa gatcaatggg
      541 cagcagcaat gactctgcgc acggtattat tgtcattgca agcgctgttg gcggctgcag
      601 aaccagatga cccccaagat gcagtagtag cgaatcagta caaacagaat cctgaaatgt
      661 tcaagcagac agctcgactt tgggcacacg tgtacgctgg agcaccagtt tctagtccag
      721 aatacaccaa aaaaatagaa aacctctgtg ctatgggttt tgataggaac gcagtaatag
      781 tggccttgtc ttcaaaatca tgggatgtag agactgcaac agaactgctt ctgagtaact
      841 gaggcacagg agctgcgcca gcatcgctct cgcctcttga ggagcatcaa cacctgttat
      901 ttttaggatt ctgcataaag ttcttttaaa ctggcattct tgcctaatga tgttacctag
      961 gcaccattgg agactgcaaa aaaaaaaatt ccctgctctg taaataaagc taattaaacg
     1021 tctgtgtaaa tttaaaaagg ggaaatactt ttaatttttt ttcttaatag tgtaaaaatt
     1081 ctttgagcta agctaaaacc atgggaaaac atgctacttt agtgtttagc agtgtaacag
     1141 actcgagagt ttgcttcagg gttctgttga ctaactagga taacactcga gtgtcggccc
     1201 ttcacattgt ggtgtagaat cacagctccc ttaagtctga acacagatcc tctgtgcctg
     1261 tgaatttact tgcatgcttg tacttgactt cttagaacgg gtagccgaaa agaccaccct
     1321 tttaagcatt aggacattct tgaattatga gatttatcct gatttaaagg tggtgaccta
     1381 atcagagctt tttagtccct gtcaccatcc agagcggaga ttctcatggt caatttattt
     1441 tgtgtcttgt gggaaacgtg ctgaggaaca gtgtcccttg ttccactttt agctcatagg
     1501 tttggaatgt tttgtccgag ttcttcactt tgtaagtagt aatgtaaaaa ggggctgcca
     1561 attttatttc tctcacaaaa ggaataaata cttgatgttg cagtatttct agatttaata
     1621 aaaggacact gcttagtttt tcagtgaatt tgacattaat ttttaaaatg ataaagtctt
     1681 tggaaacttg aagggtgcag tacctgttcc ctagtgctca gcctgacagt ttcctttgtc
     1741 agacacttga cttactcctc ctgctcccca tggtgaccca tgaaagctgt gctgtctcac
     1801 tgtatatacc agatgcacat aggaacaggg gtgtattcta aatctagctt tctttatgat
     1861 gtttctgata atgtgagaat tgaaacttta ccttctcttg tacataaact tcctgtcctt
     1921 agcaactgtc gcatctcatt ctatcttcca aagcttgaca attataagtt ttacaaactc
     1981 aaatacttcc tttaaccatc ctaattaatg ttcaagtgaa cttaggcagt tgttacatag
     2041 gagatacaat taaaaatttt gaggaccaat gcatagatta cagaaatgta atggaatcag
     2101 actaaattaa agtaaggttg tatgtatatt gaaagtcata ttctaaaagg tttgttttct
     2161 tgaagtattg tttcacctta cagtcatgaa gtgtttcaaa aatacttttt gaacctatta
     2221 tctcagcata acttcacttt acttttaata atcttgatac tacaagcaat aaaagaagca
     2281 ttaatatatt ataacattca agatgtgacg gctcttccag cgacttgtaa ggctgtcaca
     2341 gttctcagtg actgagggcc actgtcctcc acactaaact caagctgcta tgtttttctc
     2401 catgtttgcg gatgcacaca tttcctgttg cagctacatc cttttattgc tgtaggttat
     2461 tttataaaga tgccaaagga aacaagtttt tctttattta ttttaagtag ctaaaacttt
     2521 gtactgttct ttatattctc agataagaag gaaatgtgtg aaataaccat gagtttacct
     2581 ccatagttca ttagaacttt taatggaggg tggcttggta acactaatat ttaggtattg
     2641 gaaagtataa cacatgttct tggagtttac tctctgcctg tattgtcagg gtacattgtc
     2701 tagtagatga gagctcagac aacaaaagtc aggcagtacc tactttgtct tggtggcagc
     2761 tgtgttcttt ttttgttttt tgtttttttg tttttttgtt tttttgcata ttggcttcag
     2821 actggctcca ctgaatactt actggagatg tatatataac atgtggtttc atagatttga
     2881 cgtgattatg ccatcgcctc ctgtcagaac cgtgtgctca agactgtgtg ggattcataa
     2941 gcaagactgc ttggggtttc tcatgttcat ttaatgtgct aatcaccctt ttggggtatt
     3001 ttccctcaca cttctcgttt gttttttatt tttcttttgt ttcccaactc gtaggtttag
     3061 aaagaagcag cttggtcctt atcctggtgt ttcatacagt taggaaaaca atgcgaaaaa
     3121 ttaagattta gtcaaaagta ccctgatgga aattattttt tttagtctgc gtttgacagt
     3181 ttttcatttt aacaagtact acacaggttc tgttttggtg taggtgtgat ggcgctttta
     3241 ctaccaggtt cttgaaagag aaaggcaggc agagtctgtg agaggtgtat gcccagtctt
     3301 agccccctgt tcccataatt ttagtgaaat cgcgtttaga gcaggctgac tccctcatcc
     3361 tcgctgatcc tctgatcagc ttttctttct gagcctaggc ctgactttta gtataaaact
     3421 tagagagcat gttgggaact tgggattcaa aaaacatgtc agtgtttgga attgaatatt
     3481 ttacctttga gaagtgtaac taacctatca tatgaattaa accatccttt ttgaaatttt
     3541 tttttatatt tctgattgtt ggtggcattt aacactatgc aaaaaactta agtatctgca
     3601 gaaaggaact gacagtaggt tttgctcacg cggttagaca ttttgcacag cgtgttatac
     3661 gttactggtt aggctgttgg gccagtttac agaggttagg atgttgggta agtggagccc
     3721 ggatgtgatt gtaggtctca tacagctttt aaaactgcag gagaagtaga tgttcttgca
     3781 gcctactgta gcagcaccgg aaatgacgtt ttggtggggt aagggtttga aatgaatttg
     3841 ggaattgagc agtggagtca ggtatattgt ataaagatgg gatcagtgtt tagataagaa
     3901 aaaggtagga aggtgtctgg tcatagttac tttatttcat ggactttgat aagatagttc
     3961 tgtattaatc actttttcta gtttcaagga gttgatgttg gaagttacat tttcatcttt
     4021 cagtacctgt cttagtgtgt acccatcagc ctgttggtgt cactaaggga tagccttttc
     4081 tcaagcaaga cttccagtgt ctacacatgt tgtgacaaac ctacatacag cataaggtag
     4141 cgggtgatgt tggtgtctcc ttacctatct tgtgactgct tccttagact gctagaaact
     4201 ccggcgcctg cagtcatcac tggaaggtgt catgattgtg tcgcctttgg cgtagttatc
     4261 cccccacccc acccctagcc gtctatgatg aggtgtggcc aggtctgttt ggttctgagg
     4321 caagtggatc aggtcatagc actgcttttt ctgatcgtgt ctcctattgt cacctacttt
     4381 ttacaggcat cataacaaaa gctagacaat ttctttgggg gaaaaaaaag acttatttaa
     4441 tgtaatttaa agacttttcc aatagaaaat gaaatattat tgaaaatgat atctattttt
     4501 tagctttcag catttgatgg tgctttgtcg tggtgtctgc tggaagttac tgccattata
     4561 gggattttca ttaacctcac tttgaaagaa ccttgcttgt tggcttcttt agatcttaaa
     4621 cagtttgtta gtgatggtaa ttctgtagga gggtctattc tgagccatta acttcttgta
     4681 aggggaaaat gggctggtta ccagaaatat tgaagcaggg tgggctgtgg gtggggggtt
     4741 gggtgtctgt cttgagaatt aaaaactacg aaacactttt gtatacaact gaatttttaa
     4801 aaaataaaca catttttaaa gatgttgaat tttccccatt attgggaatt cttaaaaata
     4861 aatgcatgca cattccccct gc
//
