LOCUS       NM_021402               3520 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2J 2 (Ube2j2),
            transcript variant 1, mRNA.
ACCESSION   NM_021402
VERSION     NM_021402.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wang,X., Herr,R.A., Rabelink,M., Hoeben,R.C., Wiertz,E.J. and
            Hansen,T.H.
  TITLE     Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated
            degradation substrates
  JOURNAL   J Cell Biol 187 (5), 655-668 (2009)
   PUBMED   19951915
  REMARK    GeneRIF: Ube2j2 is the primary cellular E2 recruited by the mK3
            ligase, and this E2-E3 pair is capable of conjugating Ub on lysine
            or serine residues of substrates.
REFERENCE   3  (bases 1 to 3520)
  AUTHORS   Tabakoff,B., Saba,L., Kechris,K., Hu,W., Bhave,S.V., Finn,D.A.,
            Grahame,N.J. and Hoffman,P.L.
  TITLE     The genomic determinants of alcohol preference in mice
  JOURNAL   Mamm Genome 19 (5), 352-365 (2008)
   PUBMED   18563486
REFERENCE   4  (bases 1 to 3520)
  AUTHORS   Wu,C.J., Conze,D.B., Li,X., Ying,S.X., Hanover,J.A. and
            Ashwell,J.D.
  TITLE     TNF-alpha induced c-IAP1/TRAF2 complex translocation to a
            Ubc6-containing compartment and TRAF2 ubiquitination
  JOURNAL   EMBO J 24 (10), 1886-1898 (2005)
   PUBMED   15861135
REFERENCE   5  (bases 1 to 3520)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 3520)
  AUTHORS   Lenk,U., Yu,H., Walter,J., Gelman,M.S., Hartmann,E., Kopito,R.R.
            and Sommer,T.
  TITLE     A role for mammalian Ubc6 homologues in ER-associated protein
            degradation
  JOURNAL   J Cell Sci 115 (Pt 14), 3007-3014 (2002)
   PUBMED   12082160
REFERENCE   7  (bases 1 to 3520)
  AUTHORS   Bachmanov,A.A., Li,X., Reed,D.R., Ohmen,J.D., Li,S., Chen,Z.,
            Tordoff,M.G., de Jong,P.J., Wu,C., West,D.B., Chatterjee,A.,
            Ross,D.A. and Beauchamp,G.K.
  TITLE     Positional cloning of the mouse saccharin preference (Sac) locus
  JOURNAL   Chem Senses 26 (7), 925-933 (2001)
   PUBMED   11555487
REFERENCE   8  (bases 1 to 3520)
  AUTHORS   Tiwari,S. and Weissman,A.M.
  TITLE     Endoplasmic reticulum (ER)-associated degradation of T cell
            receptor subunits. Involvement of ER-associated
            ubiquitin-conjugating enzymes (E2s)
  JOURNAL   J Biol Chem 276 (19), 16193-16200 (2001)
   PUBMED   11278356
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627204.7, AK004626.1, AK010302.1 and BC065779.1.
            
            On Sep 27, 2013 this sequence version replaced NM_021402.5.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 5 encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076265.1, SRR7974084.26321.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AL627204.7         19453-19470
            19-201              AK004626.1         3-185
            202-338             AL627204.7         19654-19790
            339-501             AK010302.1         147-309
            502-888             AK004626.1         186-572
            889-1630            BC065779.1         653-1394
            1631-3520           AL627204.7         33355-35244
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 87.66 cM"
     gene            1..3520
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /note="ubiquitin-conjugating enzyme E2J 2"
                     /db_xref="GeneID:140499"
                     /db_xref="MGI:MGI:2153608"
     exon            1..338
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            339..501
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     CDS             466..1281
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /EC_number="2.3.2.23"
                     /note="isoform a is encoded by transcript variant 1;
                     non-canonical ubiquitin-conjugating enzyme 2; ubiquitin
                     conjugating enzyme 6; Ubc6p homolog; ubiquitin-conjugating
                     enzyme E2, J2 homolog"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 J2 isoform a"
                     /protein_id="NP_067377.4"
                     /db_xref="CCDS:CCDS19050.1"
                     /db_xref="GeneID:140499"
                     /db_xref="MGI:MGI:2153608"
                     /translation="MHQAKALDSARKMSNNSNKRAPTTATQRLKQDYLRIKKDPVPYI
                     CAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCN
                     TRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKKQLAAQSLV
                     FNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGELHRGQHGIQLLN
                     GHAPAAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE"
     exon            502..632
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            633..673
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            674..776
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            777..915
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            916..996
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            997..3520
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1541..1546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /note="hexamer: AGTAAA"
     polyA_site      1560
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /note="major polyA site"
     regulatory      1607..1612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /note="hexamer: TATAAA"
     polyA_site      1625
                     /gene="Ube2j2"
                     /gene_synonym="1200007B18Rik; 2400008G19Rik;
                     5730472G04Rik; NCUBE-2; Ubc6; Ubc6p"
                     /note="major polyA site"
ORIGIN      
        1 gaagcggaag cgggcctagg ccgtccccgg agcggccatc ttggaggctg agcggcggta
       61 gcggcggcga ggctgcttgc ggcgggtgcg gtgggccgag tcccagcgcg ggtgcggctc
      121 acggcgcggg ccccactctt ccgcaccctg ctctggcctc gaccacggcg aacctgaacg
      181 cggccgcggc ctcagggaga ggtgagcgct cgcgggctgc gcggggcctc gggccgctcg
      241 ttcgccccgc cggagccggc tgcagcggcg ggcggggacg cggggacgcc acacgtcggg
      301 tcccgttggt tacgtcgccg gctgtgcccg gacccgaggt gctgggcaag ttgagctctc
      361 tggcccacat ggcttctgaa gctaagaact tggggacaat gctttctgga agcatttctt
      421 ggccatgctg gactgtaacc ttgttcctgg tgtgagcatt cttggatgca tcaggcaaaa
      481 gcccttgatt ctgccagaaa gatgagcaat aacagcaata agagagctcc aacaacagca
      541 acacaaaggt tgaagcagga ctaccttcga attaagaaag acccagtgcc ttacatctgt
      601 gcagagcccc tcccttcaaa tattcttgaa tggcattatg ttgtccgagg ccctgagatg
      661 actccttatg aaggtggcta ttatcatgga aaactaattt ttcccagaga atttccattt
      721 aagcctccta gtatttacat gataaccccc aatggaagat ttaagtgcaa cacaaggctg
      781 tgtctctcca tcacagattt ccacccagat acatggaatc cagcgtggtc cgtctccacc
      841 attctaacag ggctcctgag ctttatggtg gagaaaggcc ccaccctggg gagcatagag
      901 acgtcggact tcacgaaaaa acagctggca gcacagagtc tagtgtttaa tttaaaagat
      961 aaagtctttt gtgaattatt tcctgaagtt gtggaggaaa ttaaacagaa acagaaagca
     1021 caagatgaac taagtaacag gccccagaat cttcctttgc cagacgtggt tcctgatggg
     1081 gagctgcacc gtggccagca tgggatccag ctcctcaatg ggcacgcacc cgctgctggc
     1141 cccaacctcg ctgggctccc acaggccaac aggcatcacg gactcctggg cggcgcgttg
     1201 gcgaacttgt ttgttatagt tgggtttgca gccttcgcct acacggtcaa gtacgtgctg
     1261 agaagcatag cacaggagtg agggtcacaa gccacagcct acagttgctg ccgagggaca
     1321 cagggccaga gcttgatgcc ggcgtgaagg acgtgctggc acagggtggc ctgccggact
     1381 cccgggcttc actttttaca aaccttaaag agaaagcaaa aaccaaaatg tgatctggag
     1441 gagactggtg gttctcaact ccctcttgag gttgactgga cggagccact cgcgctctgg
     1501 ttttactgct tatctcctgt gttgtctttt gaccctgttt agtaaacctg ttttgttggt
     1561 tttgttttgt tttgtttttc attttattag atttggtcac ttaaaatata aaactttaat
     1621 gcctatcaaa tactctgtgt gtgttcttag tggaggagct gaaagcctcc tggtggggct
     1681 aagtgggccg gctggtctag gagaggactg atttgatctc attgtaaaat tgcttctgag
     1741 tgagagaaga ctcacacaaa gtgccggttc tgctttctgt taaggtagaa gcagcagctt
     1801 gctctctgac cacaaagtgc tgcacttaga accgtggctg aggactgtgg tgaaggactg
     1861 tgggtagccc aggtactgct gtcaacctta gtacctctgc tttcagctcc cttgagtgat
     1921 ggagaagtag gagccttggt aaccctggtt ccttatgtcc agttggccag aagccggccc
     1981 tcctatgttg gcagacagtg cactccaagt tctttcatat cttacggtag gctacaccat
     2041 cctttccggg tgacctatgt aagatgtcgc tctctacaga tcatggagag ggaaactgcc
     2101 cagaacacgt caccaggcca ccagtaagct ggcaagctcc cttatggatg tcatctcttt
     2161 gagattttgc ttaatgtacc tgcagtagtt gtttgggaag gctaacatcg tctgtgttgg
     2221 gcatcaatca tcaccagggg ctcctaaaag ccttctaagc ccacctcctt tcaggggcca
     2281 tggtaaagct gaagtgctcc catgtgatga ttacctgtaa ttcagctctg tggtttacaa
     2341 ctagtatgta tataccatgt agtcctctcc taaaacggtc tttagttacc cctttgctct
     2401 gaactccacg atgtctgtgt taatgtccct tccctggtag gctgtgacca agtgcaatgc
     2461 ctggcttgtg agaagggaat gttgcagctg ccaaaaggta gattgacaac ccttggttgg
     2521 gcctgtgcaa cctgtgcagc cttcggtggg tggagcccta cagacatgtg cttcctacta
     2581 ttggtagatg gtaatttttc cctggtccca gagtgctccg ttgcaaatta cctcaattcc
     2641 aaattggtgt ttcctgaagt tcttgaaaga cagaacatta tcgaatttag tcctctgaaa
     2701 tctcagcacc acccccagcc ccctggcccc agcatggcct ttatgggagt ttagggattt
     2761 caagacaggg tttctctgtg tgtccccagc tgtcctagaa ctcactctat aaatcaagct
     2821 gtccttcatc tcagatccac gtgcctttgc ctcccaagag ctaggattca aggtgtgggc
     2881 ctccattgcc caactgaaag ctcaactttg attttaaaaa atttttaaga tttatttatt
     2941 ttattatatg taagtacact agctgtcttc agacacttca gaagagggtg tcagatggtt
     3001 gtgaaccacc atgtggttgc tgggatttga actcaggacc tctggaagag cagtcagtac
     3061 tcttaaccac tgagccatct caccagccct tttttaaaat ttttaattgg ttattttgtt
     3121 tatttacatt tcaattgtta tcccccttcc cggttttctc tccaaagaac ccccatccca
     3181 tcctcctgcc tccatgaggg tgctccccca cccagtcctg ccccaccacc ctactgccct
     3241 tcactggggc atcaagcctt cacaggacca aactcttaac agctcagaag gaaagaaaat
     3301 tttaggaagt gcttgttctt agggtagaag taaaaagcag cctgctctct ggccacaagg
     3361 tgtcgcactt agtgtacgac tgaagagtgc tgtgccggct tggcaggtta gccccaacac
     3421 ttctcccagc tttgagtgct acaggaccct agagtgtcca ctgtgtgttc cgtgctctga
     3481 cccccgaata aaaggtggcc tttttaacca ttggctcaag
//
