LOCUS       NM_019586               3570 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1),
            transcript variant 1, mRNA.
ACCESSION   NM_019586
VERSION     NM_019586.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Nozawa,K., Fujihara,Y., Devlin,D.J., Deras,R.E., Kent,K.,
            Larina,I.V., Umezu,K., Yu,Z., Sutton,C.M., Ye,Q., Dean,L.K.,
            Emori,C., Ikawa,M., Garcia,T.X. and Matzuk,M.M.
  TITLE     The testis-specific E3 ubiquitin ligase RNF133 is required for
            fecundity in mice
  JOURNAL   BMC Biol 20 (1), 161 (2022)
   PUBMED   35831855
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3570)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3570)
  AUTHORS   Hagiwara,M., Ling,J., Koenig,P.A. and Ploegh,H.L.
  TITLE     Posttranscriptional Regulation of Glycoprotein Quality Control in
            the Endoplasmic Reticulum Is Controlled by the E2 Ub-Conjugating
            Enzyme UBC6e
  JOURNAL   Mol Cell 63 (5), 753-767 (2016)
   PUBMED   27570074
REFERENCE   5  (bases 1 to 3570)
  AUTHORS   Koenig,P.A., Nicholls,P.K., Schmidt,F.I., Hagiwara,M., Maruyama,T.,
            Frydman,G.H., Watson,N., Page,D.C. and Ploegh,H.L.
  TITLE     The E2 ubiquitin-conjugating enzyme UBE2J1 is required for
            spermiogenesis in mice
  JOURNAL   J Biol Chem 289 (50), 34490-34502 (2014)
   PUBMED   25320092
  REMARK    GeneRIF: UBE2J1 is required for spermiogenesis in mice
REFERENCE   6  (bases 1 to 3570)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 3570)
  AUTHORS   Menon,M.B., Tiedje,C., Lafera,J., Ronkina,N., Konen,T.,
            Kotlyarov,A. and Gaestel,M.
  TITLE     Endoplasmic reticulum-associated ubiquitin-conjugating enzyme
            Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in
            MK2-mediated TNFalpha production
  JOURNAL   Biochem J 456 (2), 163-172 (2013)
   PUBMED   24020373
  REMARK    GeneRIF: Ube2j1 is phosphorylated in response to
            lipopolysaccharides in macrophages and contributes to MK2-dependent
            TNFalpha biosynthesis by an unknown mechanism.
            Erratum:[Biochem J. 2014 Jan 1;457(1):229]
REFERENCE   8  (bases 1 to 3570)
  AUTHORS   Lee,J., Xu,Y., Chen,Y., Sprung,R., Kim,S.C., Xie,S. and Zhao,Y.
  TITLE     Mitochondrial phosphoproteome revealed by an improved IMAC method
            and MS/MS/MS
  JOURNAL   Mol Cell Proteomics 6 (4), 669-676 (2007)
   PUBMED   17208939
REFERENCE   9  (bases 1 to 3570)
  AUTHORS   Lester,D., Farquharson,C., Russell,G. and Houston,B.
  TITLE     Identification of a family of noncanonical ubiquitin-conjugating
            enzymes structurally related to yeast UBC6
  JOURNAL   Biochem Biophys Res Commun 269 (2), 474-480 (2000)
   PUBMED   10708578
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772288.12, BY707310.1, AK149985.1, AL670464.12, AW457749.1,
            BY416821.1 and CJ279553.1.
            
            On Sep 20, 2017 this sequence version replaced NM_019586.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149985.1, SRR7974084.3323.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AL772288.12        115678-115722
            46-137              BY707310.1         1-92
            138-1029            AK149985.1         4-895
            1030-1030           AL670464.12        199449-199449       c
            1031-1245           AK149985.1         897-1111
            1246-1246           AL670464.12        199233-199233       c
            1247-3405           AK149985.1         1113-3271
            3406-3566           AW457749.1         16-176              c
            3567-3567           BY416821.1         368-368
            3568-3570           CJ279553.1         409-411
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 14.61 cM"
     gene            1..3570
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /note="ubiquitin-conjugating enzyme E2J 1"
                     /db_xref="GeneID:56228"
                     /db_xref="MGI:MGI:1926245"
     exon            1..237
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1163
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     non-canonical ubiquitin-conjugating enzyme 1;
                     ubiquitin-conjugating enzyme E2, J1; E2
                     ubiquitin-conjugating enzyme J1"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 J1 isoform 1"
                     /protein_id="NP_062532.2"
                     /db_xref="CCDS:CCDS18019.1"
                     /db_xref="GeneID:56228"
                     /db_xref="MGI:MGI:1926245"
                     /translation="METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFT
                     VRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETW
                     QPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVL
                     LPLKSGSGSSQADQEAKELARQISFKAEVNSSGKTIAESDLNQCFSLNDSQDDLPTTF
                     QGATASTSYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQP
                     PRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL"
     exon            238..311
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            312..443
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            444..528
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            529..634
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            635..764
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            765..884
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     exon            885..3570
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1203..1208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /note="hexamer: ATTAAA"
     polyA_site      1232
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /note="major polyA site"
     regulatory      3524..3529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /note="hexamer: AATAAA"
     polyA_site      3566
                     /gene="Ube2j1"
                     /gene_synonym="0710008M05Rik; 1110030I22Rik; Ncube;
                     NCUBE-1; Ncube1; Ubc6p"
                     /note="major polyA site"
ORIGIN      
        1 tcccgcaggc cgtccgcagc ccggcgcccc gttcggcgac gccccggcgc ccggccggcc
       61 ctcagggagg cggaccccgc ggcacgcacc tcgcccgcgg tcgggctgga ctgggcggtg
      121 tcagcggagg ccctcgctag gcgcgtccgc cgcgcgagac ccgggcagct gcggcgccga
      181 cgagagcggc agcggggacg tccgccatgg agacccgcta caacctgaag agcccggccg
      241 ttaagcgatt gatgaaagaa gctgcagaat tgaaagatcc aacagatcat taccacgctc
      301 agcctctgga ggataacctt tttgaatggc acttcacagt tagagggccc ccagattccg
      361 attttgatgg aggagtttac catggacgca tagtactgcc accagagtac cccatgaaac
      421 caccaagcat tatcctgcta acagctaatg gacgatttga agtaggcaag aagatctgtt
      481 taagcatctc aggacatcat cctgaaacct ggcagccttc atggagcata aggacagcct
      541 tgctggccat catcgggttt atgccgacta aaggagaggg ggccataggt tctctagatt
      601 acacacccga ggagagaaga gcgcttgcca aaaaatcgca agatttctgt tgtgaaggct
      661 gtggctctgc catgaaggat gtcctgttgc cattaaaatc tggaagtggt tcaagccagg
      721 ctgaccaaga agccaaagaa ctggccaggc agattagttt taaggccgaa gtcaattcat
      781 ctggaaagac tatcgctgaa tcagacttaa accagtgttt ctcactaaat gactcacaag
      841 acgatttacc tacaacattc cagggtgcta cagcaagcac atcgtatggc gcccagaatc
      901 catcaggagc acccttgcca caacctaccc agccagctcc taagaatacc tccatgagcc
      961 ctcggcagcg cagagcccag caacagagtc agagaaggcc atccacttcc cccgatgtgc
     1021 tccaaggcca gccaccaaga gcccaccaca cagagcacgg cgggtccgcc atgctgatca
     1081 tcatcctgac tttagcattg gcggctctta tattccgacg aatatatctg gccaatgagt
     1141 acatatttga ctttgagtta taatgctggt ttgtgactta agagctgtga ctcagttgct
     1201 tcattaaaca ttctgcattg ggtataacct aaggattgtt tacaaaaaga ttattttgta
     1261 tttaccctcc attacttttt tattgttata aattcatttg ctataagtag acattcaggc
     1321 tgtggtccaa aagtttgtcc tgcctaggtt atggactgga agccatagtt ccttgtctta
     1381 ctgtttaatc tacgtcacaa acaagtaact tattgtggtt gctcacagcc aagctgcttt
     1441 tctatgtagt cctttgcatt ttcagataaa agaaggtgtt tccacattgg agtattactg
     1501 ggtactcagt ctcccttgct gagtactgtc tttagttata tagaccaaga gggatgaagg
     1561 atgggggagg ggcactgctc actagcttct tgagaagtgt catttatagt agagacaggg
     1621 gagtgcccac tctgtgaact ttgtatttga aactttgtgt ggtgagaata attttatttt
     1681 agacattcat gaagatcttt gttatatttt tgtttttgga tattacttga agaggaaaga
     1741 ctttgtagcc actttggtat gcaccatctc caatatgcac acaaatgaaa agaaagcggg
     1801 ctttcataac ctgtctcaag gaaggaaggg tccaagccag aagttgtttt atttttcttt
     1861 tacaagtagg taaggagatc ttaaagtttg agtctttact ttcatttgta catggtttga
     1921 attatatgcc cagctcataa tcactatagc ttttcattat tccagaaata tgcttagtat
     1981 tcagatttta atagaaaaac tcagggccca aataacagtg atagattaac cacaacaaac
     2041 ctttacttag aattgttcac tagtcaaggc ctaagaaaaa tttcatgaaa agtctatctt
     2101 tgccttggcg ccagcaagtc cccccatgct gacgcgtgta gctctgtcat ctcagtgact
     2161 agtgagactg cttaggacaa cgtcgtcgtc agcatgctcc ctcagcaacc ttgagggcac
     2221 tgtcgagggc cccacattgc ccatttgact ggaaaatata ggcagtcccc agcttttaaa
     2281 tatatcatgc tctcaaagcc catttagaag tttttgtttt gtttttgttt ttaaggacaa
     2341 ctgaatataa atggtgtttg ggttctagct cagtccacaa cggtcagttc atatgtcttt
     2401 taagtatatg tgggagggga gttgtaaagt cctcatggtg ttttaactaa atataattat
     2461 gggcaagaaa aaaacagctc acaaaatcat tctaaaggtg agcttaattt tcactcctgt
     2521 catgtgatgt tgtggtctgt taccactgaa gataattttc atttgtattt ggtagtcagg
     2581 ttttgttgtt actgttgtta tcccaacacc taaagtttta aaaggcactg agtgctagac
     2641 gataatctcc cagtttaata gagtagattt tatagttatt taaggattgt ttattgatga
     2701 ggttggtaaa atctagaaag gccaaggact ttgtgaagac ttcatgtaag gaagactata
     2761 ggttaccatt ttaaaaataa tgttcttgct ttgaaaaagt atattttgag gtcttagttt
     2821 ataatatgag aaacttgggg tccatgatct agtaagaagt cagtaagcca atgggaagcc
     2881 tgggtggcct tcccacttag gagaagaacc ctgatggtgt gccccaggcc gagatggctt
     2941 gtgtgctaga gcagtcctcc ccaagtcagg cccggctagc cttggggctc tgccatcttg
     3001 ctgtgtccac atggacccct gtgccaggtg gcgagggttg ttccaagatc ttatccaccc
     3061 cctcactttt gctaactggc tacctcctta cgtgtgttgg tattttttag cacctgatac
     3121 acatatcaaa gtggtacatt ttgcttttac ccctgatatg ctatttaaaa attgtcctcc
     3181 aacccccacc ttggagctag tgagctccta gaaagggcct ctgttgtcag agcctcaggt
     3241 taactcccta gatccagtat aaggcacaaa ccagggagaa actggtaggt agtcttcaat
     3301 ccttgtttcc ccagttctca gagttcagaa ctcaaggctt cttcactgat cagcatagca
     3361 catgagtaca catcaaatta gttcaaatag catgcttgaa aaaaatctgc ctgttttgct
     3421 tgtagcagtc tggcccatga gccaaccgaa ccttctgtac gtgggtgggg ctttgatgta
     3481 gcactttaca tttccctgct gctgctgttg tttattctac tgaaataaat gctttcacca
     3541 agctgcttgg tgaatttaaa aactaagtta
//
