LOCUS       NM_009459               4657 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript
            variant 1, mRNA.
ACCESSION   NM_009459 XM_913498
VERSION     NM_009459.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 4657)
  AUTHORS   Yi,J.S., Park,J.S., Ham,Y.M., Nguyen,N., Lee,N.R., Hong,J.,
            Kim,B.W., Lee,H., Lee,C.S., Jeong,B.C., Song,H.K., Cho,H.,
            Kim,Y.K., Lee,J.S., Park,K.S., Shin,H., Choi,I., Lee,S.H.,
            Park,W.J., Park,S.Y., Choi,C.S., Lin,P., Karunasiri,M., Tan,T.,
            Duann,P., Zhu,H., Ma,J. and Ko,Y.G.
  TITLE     MG53-induced IRS-1 ubiquitination negatively regulates skeletal
            myogenesis and insulin signalling
  JOURNAL   Nat Commun 4, 2354 (2013)
   PUBMED   23965929
  REMARK    GeneRIF: MG53 is an ubiquitin E3 ligase that induces IRS-1
            ubiquitination with the help of an E2-conjugating enzyme, UBE2H.
REFERENCE   4  (bases 1 to 4657)
  AUTHORS   Kim,K.I., Giannakopoulos,N.V., Virgin,H.W. and Zhang,D.E.
  TITLE     Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme
            for protein ISGylation
  JOURNAL   Mol Cell Biol 24 (21), 9592-9600 (2004)
   PUBMED   15485925
REFERENCE   5  (bases 1 to 4657)
  AUTHORS   Thomas,J.W., Lee-Lin,S.Q. and Green,E.D.
  TITLE     Human-mouse comparative mapping of the genomic region containing
            CDK6: localization of an evolutionary breakpoint
  JOURNAL   Mamm Genome 10 (7), 764-767 (1999)
   PUBMED   10384057
REFERENCE   6  (bases 1 to 4657)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   7  (bases 1 to 4657)
  AUTHORS   Wefes,I., Kaiser,P., Schneider,R., Pickart,C.M. and Finley,D.
  TITLE     Characterization of a cDNA clone encoding E2-20K, a murine
            ubiquitin-conjugating enzyme
  JOURNAL   Gene 163 (2), 321-322 (1995)
   PUBMED   7590289
REFERENCE   8  (bases 1 to 4657)
  AUTHORS   Wefes,I., Mastrandrea,L.D., Haldeman,M., Koury,S.T., Tamburlin,J.,
            Pickart,C.M. and Finley,D.
  TITLE     Induction of ubiquitin-conjugating enzymes during terminal
            erythroid differentiation
  JOURNAL   Proc Natl Acad Sci U S A 92 (11), 4982-4986 (1995)
   PUBMED   7761435
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF539343.1, AK131688.1 and AC161538.7.
            
            On Dec 17, 2009 this sequence version replaced NM_009459.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008517.1, U19854.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                CF539343.1         1-22
            23-1134             AK131688.1         1-1112
            1135-4657           AC161538.7         87334-90856
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.52 cM"
     gene            1..4657
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /note="ubiquitin-conjugating enzyme E2H"
                     /db_xref="GeneID:22214"
                     /db_xref="MGI:MGI:104632"
     exon            1..488
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     CDS             436..987
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UBCH2; ubiquitin-protein ligase H; ubiquitin carrier
                     protein H; ubiquitin-conjugating enzyme E2-20K; E2
                     ubiquitin-conjugating enzyme H; (E3-independent) E2
                     ubiquitin-conjugating enzyme H"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 H isoform 1"
                     /protein_id="NP_033485.1"
                     /db_xref="CCDS:CCDS19969.1"
                     /db_xref="GeneID:22214"
                     /db_xref="MGI:MGI:104632"
                     /translation="MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGT
                     PYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLT
                     NIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEG
                     TGDSSSESSMSDFSEDEAQDMEL"
     exon            489..565
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     exon            566..640
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     exon            641..680
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     exon            681..733
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     exon            734..862
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     exon            863..4657
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2960..2965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /note="hexamer: AATAAA"
     polyA_site      2981
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /note="major polyA site"
     regulatory      4631..4636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /note="hexamer: AATAAA"
     polyA_site      4657
                     /gene="Ube2h"
                     /gene_synonym="1500009C23Rik; E2-20K; Gid3; Ubc8"
                     /note="major polyA site"
ORIGIN      
        1 gcgcctagag cgaggctagg gtagagaggc cgaccgagct ggagtcgtcg gcttgtggag
       61 gaagcagctg ggagctggcg agagcgtgag cgagcgggcg ggcaggcgaa cgagctagcg
      121 caggcgggag ggagcggcgc acggcgcgga gcagcggcag cccgggccgc ggcggagcct
      181 cagtcggagg gcagcccgag cgggcgcctc ggtccccgcc cccactcggc ggggcagtgc
      241 ccggtgttcc cccgtgcggg gggcggcggc tgagcggacc ccacgggacc gcgcgggtgt
      301 tgccacctcg gccgaggagc cggcgaggcc gcggggccct gcgagctggg cccggagccc
      361 gtgacagacg ggccaaggag gggagagagg cggcggcggc gcaggcgacg gggaggcagc
      421 ggcagcggcg acaccatgtc atcccccagt cccggcaaga ggcggatgga cacggacgta
      481 gtcaagctca ttgaaagcaa acatgaggtt acaatcctag gaggactcaa tgaattcgtt
      541 gtcaagtttt acggaccaca aggaacacca tatgaaggcg gggtgtggaa agtgagagtg
      601 gaccttcctg ataaataccc tttcaagtct ccatctatag gattcatgaa taaaattttc
      661 catcccaaca ttgatgaagc gtcaggaact gtgtgtctag atgtaattaa tcaaacttgg
      721 acagctctct atgatcttac caatatattt gagtccttcc tgccccagtt gctggcctat
      781 cctaacccca tagatcctct caatggtgac gctgcagcca tgtacctcca ccgaccagaa
      841 gagtacaagc agaaaattaa agagtacatc cagaagtacg caacggaaga ggccctgaag
      901 gaacaggaag agggcactgg cgacagctca tcggagagtt ctatgtctga cttctcagaa
      961 gatgaggccc aggacatgga gttgtagtag aaaaggcatc tgcttttcag aaagactatt
     1021 atttcctaac catgagaagc agactctaat attcatattt aaacaaagca atttttttta
     1081 ttactaaaca aggtttttat gaataatagc attgatatat atatattata tatcaccctt
     1141 tagatcttga tttcttggtc atttctcaac ctgaggtgca tagcatattc ccacattcca
     1201 ttttggtagc aatatgcagt ccgaatgcat gcattcataa gtccatttgg ccaagtcagc
     1261 ctgtgtgcta ctgaactgtc aagagattgc cactctcata ggtaaacatg aggaaaacag
     1321 gaaaagtttg cttcttttta ttgatggttc caaagaaaca aaccaaatct accagctctt
     1381 ggatgaagat agaacagtgc ttttccagag tgggaaggaa ggggttgaga cgcgtgcttt
     1441 gagagcctgt gaggctgctc cgtccttgcc agctgctccc tagtcctgag tggtccttgg
     1501 tgaggagcag caagatgcag cgagttgagt gagacttagg gcttttgatt tttttttttt
     1561 ttttttttga ctcggattta agtgcatttt tctaggtaga ctcctgccct ccttgctacc
     1621 agacctgcag ccaccttggt tttgccttgg agaacatttg gggagcattt cctgaaccca
     1681 tgtcttttgg gggtacagct tgtcatccag caggaattat tgagcgagca ggactggagg
     1741 agacgggaat gaagacacaa gagtgacatc tgatcctagc acggggcctg ttgatccatt
     1801 gcctggactg agctgtgaaa ctggacgcgg ttttggtttt ttgttttgtg tttctttttt
     1861 cttaatttgt gtgaattgta cctagacagg ttacagattt aattttcacc taccaaggga
     1921 cagtctcctc cctggacaag gctcagtgga ccatcagatg gaggaagact ggccccattg
     1981 cttgagcccc tgtgcgaccc ttgacctcca gccttagttc tcctggaagc tgccaactgc
     2041 ttacgggagt gggtagattg ggcgtctgag acccagaaca tcctaaattt gcaatgggta
     2101 acagctgaaa caaacggaag tttttgtacc aaagtggccg gaggtgggga agaactactt
     2161 tcgagaattg ttacggaggc aggttgaggg gacactttga ccctccaagg taacagccag
     2221 tcagatggct tgaggtgcct ggcccatgct gccggctaaa tgccatccag tgtgcccagc
     2281 agtttcagac ctgactctgg agggaatttg gagacagtgt agacagcagc tctgctctgt
     2341 attctttttg acaaaaccag aacagtagaa gtagccaatc gtaagacctg aagacatttt
     2401 gaatgtccca ctggactaac ctttcttttg atgtgtgttt ttagtttaga agtgctatat
     2461 tccacaaggg ttaagtcaat gaggttggaa gacacctatc ttttttttta attcaaaaag
     2521 cacaatcagt ctttcctttg aaaatccata taaagtacaa cttttagcgt gattattttc
     2581 ctaaatacaa agacaaataa tttacttatt ttatgttaat tacgggcatg aagcaaaagg
     2641 tttctttggg gaacatgcag ttttgtagtg ctggggttgg ttcatgtcct ctggcaccct
     2701 ggcttgttga aaggtggctt tagctgtgtg ctgtgtacct gcttacgtgg cctgctgtcc
     2761 tctgtgtgat taggagggtt tctgctggcc ctgcagcttg cttccctgcg gattaggcct
     2821 ttgctcatgc tatagccttc atgtgggtgc tacctagagc aggcgtgaac tgctgggtgg
     2881 tgattgtgag ttcagacaat aaaaaaaatg tatttttttc aatactgtat aaaaatagta
     2941 ttctgattac ctccccctca ataaaaaaaa agtccagata attgcttttg atattgatgg
     3001 aaaaactaca aaacagacac aacaaaccag agccaagttt ggggaacggg tgtgagcagc
     3061 tctggctcct gaccctcacc gggtccgctt tgccccagtg aagagacagc attccttcct
     3121 gtgcagacct gagtgcagag tgtgtccatc tcggcgtatg gtgaaggtgt cactgggttc
     3181 tgcagcactg gagtttccat gaccgctccc acatcacccc accctcgact ctggcaccag
     3241 tatggttcta tgcatgttgc agctctctgg gctgtgactc tgttaagaag gtttgccagc
     3301 tttctggccc ttccccgctc ctagttacta gatgaccata ctcatacttg caaaggagag
     3361 gagagagtgc taaagcctgg ttctgactga gggctctgcg tccctgcagt aaacaggtga
     3421 gatctgcagc agggcagggc tgctttaaca ggcaggggtg gcagtaagtc acagctgctg
     3481 ctgctgtcgt ctgcccgcca ttcagctaac cgttctgttg aggtcctgcc ttgtgctttc
     3541 ctagtggaag ccgtcctttg cccccgttga tgacttgagc ccttgccaaa caaacaccga
     3601 tgagtgaagt ggctgcaact tggcagcccc aaaacctgcc acacccctgc acccctggaa
     3661 gcccagcacc ggggccagca ggctgtgttc cgtgcttgcc aacctagacc ctggcattag
     3721 aaattgtttt gtcagcagtc tctgccctct cgagagagat gagcagcctc aactgacgtt
     3781 tctattaatg agttatcaga agttgcaaca tctctccagc actaatgtgt tttgtccact
     3841 tcaaacgctg ttctaattgc caaccggctg ctctaggctc ctgaggagtg tcccttctca
     3901 aaggaactgc gccctggaaa gggctccccg aagttctagg tggccttgac ctgacacttg
     3961 cattttctag gccactcttt ggggtggctg agaatcatga cagaccctct gccagtctct
     4021 agtttctctg ggtcaggttt ctgagtactg gcattggatt ttaagcggca tgtgaccctt
     4081 tgtgttttag tgactcagaa aagtcaagcc atttgcaaaa gttagcagtt tgttttgtca
     4141 acttgtaact gggtcatagc cttgctgagt gaaaccaact catttctgat tgggcacaaa
     4201 gaactgcatt ctttggacgt gtgaatccat gttcctcttc ctctggccac tgaacacctc
     4261 caccagctct ttgggaattg gatggaaagg cggcccctgg gggccagggg tgtgcagctt
     4321 cccactcacg ggaggagtgg ccagggctct tggtttagca attacacagc tctaggtgcg
     4381 ggaagaaatg gcctttaatt actgattgta tttggtacat attgtgtgat acttgaagag
     4441 ataaaaggga cttaccagcc cttaattgaa atctaagctt tttaccgctt tcccctcctt
     4501 gccctctctc ctcccttctg ccctccttgc attgtgatga tctagtgggc atgtctgtca
     4561 cctggacaat tgcctcttct gactataacc tcttaatagt tgtgtataat gaaaactgta
     4621 aactttttta aataaaatgt gtatatacct tggcaaa
//
