LOCUS       NM_025985               3837 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2G 1 (Ube2g1), mRNA.
ACCESSION   NM_025985
VERSION     NM_025985.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Neupane,S., Berardinelli,S.J., Cameron,D.C., Grady,R.C.,
            Komatsu,D.E., Percival,C.J., Takeuchi,M., Ito,A., Liu,T.W.,
            Nairn,A.V., Moremen,K.W., Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type 1 repeats is essential for
            ECM remodeling and signaling during bone development
  JOURNAL   Matrix Biol 107, 77-96 (2022)
   PUBMED   35167946
REFERENCE   3  (bases 1 to 3837)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3837)
  AUTHORS   Steinmetz,C.C., Tatavarty,V., Sugino,K., Shima,Y., Joseph,A.,
            Lin,H., Rutlin,M., Lambo,M., Hempel,C.M., Okaty,B.W., Paradis,S.,
            Nelson,S.B. and Turrigiano,G.G.
  TITLE     Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting
            AMPAR into the Recycling Endosomal Pathway
  JOURNAL   Cell Rep 16 (10), 2711-2722 (2016)
   PUBMED   27568566
REFERENCE   5  (bases 1 to 3837)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3837)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   7  (bases 1 to 3837)
  AUTHORS   Ye,Y., Meyer,H.H. and Rapoport,T.A.
  TITLE     Function of the p97-Ufd1-Npl4 complex in retrotranslocation from
            the ER to the cytosol: dual recognition of nonubiquitinated
            polypeptide segments and polyubiquitin chains
  JOURNAL   J Cell Biol 162 (1), 71-84 (2003)
   PUBMED   12847084
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV657669.1, AK167472.1 and AL808023.3.
            
            On Sep 22, 2006 this sequence version replaced NM_025985.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.48353.1,
                                           SRR1660817.281194.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               DV657669.1         1-137
            138-1963            AK167472.1         4-1829
            1964-3837           AL808023.3         45778-47651
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 44.42 cM"
     gene            1..3837
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /note="ubiquitin-conjugating enzyme E2G 1"
                     /db_xref="GeneID:67128"
                     /db_xref="MGI:MGI:1914378"
     exon            1..375
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             330..842
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /EC_number="2.3.2.23"
                     /note="UBC7; E217K; ubiquitin-protein ligase G1; ubiquitin
                     carrier protein G1; ubiquitin-conjugating enzyme E2G 1
                     (UBC7 homolog, C. elegans); E2 ubiquitin-conjugating
                     enzyme G1"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 G1"
                     /protein_id="NP_080261.2"
                     /db_xref="CCDS:CCDS36217.1"
                     /db_xref="GeneID:67128"
                     /db_xref="MGI:MGI:1914378"
                     /translation="MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIG
                     PPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKY
                     GYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARC
                     VRKSQETAFE"
     exon            376..478
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            479..576
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            577..755
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            756..879
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            880..3837
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1948..1953
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /note="hexamer: AATATA"
     polyA_site      1968
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /note="major polyA site"
     regulatory      3813..3818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3837
                     /gene="Ube2g1"
                     /gene_synonym="2700059C12Rik; D130023C12Rik"
ORIGIN      
        1 gccctgcgcg cagtgaggca gtggcggggg aaggcaccgg tggggccgac gggcgggcag
       61 aggagggaag cgggcgagcg aagtcccagt gcgcgccgcg gcggcccggg taccctcccc
      121 ttccgggcgt gaggcgctga gcgaggaaag ccgagcggag cggactcgca taggcggcga
      181 ggtgcttctc gggtctccca agaccggcct ccccgcggct tcggcgctcc ggtccgggtt
      241 cctcaacaca catctagctc caggcaccgg cagagcttgt cgccagtccg tcgaagtccc
      301 ggtggcggcc cgggctctcg gtggggagga tgacggagct gcagtcggcg ctgctactgc
      361 gaagacagct ggcagaactc aacaaaaatc cagtggaagg cttttcagca ggtttaatag
      421 acgacaatga tctctatcgt tgggaagtcc ttattattgg tcctccagat acactttatg
      481 aaggtggtgt ttttaaggct catctcactt tcccaaaaga ttatcccctc cggcctccta
      541 aaatgaaatt cattacagag atttggcacc caaatgttga taaaaatggt gatgtttgca
      601 tttctattct tcatgagcct ggggaggata aatatggtta tgagaagcca gaggaacgct
      661 ggttacctat ccatactgtg gaaaccatca tgattagtgt catttctatg ctggcagatc
      721 ctaatggaga ctcacctgca aatgtagatg ctgcgaaaga atggagggaa gacagaaacg
      781 gagaatttaa aaggaaagtt gcccgctgtg taagaaaaag ccaagaaact gcttttgagt
      841 gatgtttatt caatagttag taacttcact tatttcaggg tctccaattg agaaacatgg
      901 cactgttttt cctgcactct acccacctat tgctggactt ctgttgtaat aagttggcaa
      961 gctctggctg gaactgggct gcaataaaac atgccagtca ttaatgctga caagagccta
     1021 acaagtgcca actaaagatg attacgcatt ttgaattcta atgaactgtt ttaactttca
     1081 ggaagaattg taaagacatg tacatagcac aacatgatcc ggataatata tatactgttc
     1141 atgtacatcc acaaacacac cttgtaccaa ataatgcttt cttgtagtag aataagaatc
     1201 gtgtaaattc taaaagactt tagcgggttt tcttttccca ttcattgttt tttatcagtt
     1261 caaaaagatt ccttaaagca tgtcaggtga aaaccaataa ggatgaaaag tttccatata
     1321 atttccttta aaccattgag tctttcatta cattctttcc atttactaat cttttatatt
     1381 ttctttgttt tgtcacaccc ccctttcctt ttatctctga tatctgccag aaagttctca
     1441 aatcatttag ttaaaatact gaaatactag taaattactt gagtttttca cgatgacttc
     1501 agaggagggg tcatcactag gtgctttgtc ctttttgtac tatgattcac ccatctcttg
     1561 gattggatgt agcaataaca ttttctggct gttgagagct cttgatccta gtcattatct
     1621 cctgagaact ttaaaaataa aatgtaaagg ctgttctaag ttcatcttag tgaaatttat
     1681 tccaaacaat acaaaaatcc agatttggtt tttgccctta agttgtctaa tattaaggtt
     1741 ggatgaatat agcatgcgca gctacaggct ttctacatct gggattgcta ttaaaaatgc
     1801 tatttgtcct attacccttc tccttagtcc atatttgttt cttcttacag cagattttcc
     1861 tcacctattg tacaaagaaa ttgcgatgta tattttcatg taatttgatt ttggaatttt
     1921 gtcaccttat gtagtgagtt cttccaaaat ataatttttt tccaataatt gtcaagttgt
     1981 tggcttttat tgtattaaat gaaggttata gtactgaata ccagagaagt gcagtttaga
     2041 aaaatctcag gttgattcct tatgcaaaca aacttaatac ttgaaaatct catggccatg
     2101 gcagtgtact ctcgggttct gtctacagtc tttaaatctg aaggtgttcc agaggagagt
     2161 cctttgctag ctgacattca gactctgtca ctgacatagt gcacttagat gtgatttttg
     2221 tttgagtgta aatatgtttc ctaagcagtt ttataaaaca acttcggtga tgggtaaaat
     2281 tagtacatta aatgttgaag acaggctggc ctaaactcct gtctggtttt agctgagatg
     2341 ggaagttgtg tttggtatac aagagctaga gagcataaaa cagtacttgt cacttgatgc
     2401 tgtttgagtg cgagaatagg tgtggctctg cccttggggt ttggataaat cttttggcag
     2461 aagagggaat gaaaggattc ctgtccgtgt catacctttc aacttatgtc tttttaaagc
     2521 agaggggaaa tggatcaaga tgtggctatg acacagcaac attttaaagt tttcttggaa
     2581 gcattgtaaa atcctataaa cctgaaaacc cgtaactatg gagcaactgt gaacacctca
     2641 gttttgttgg agcccaataa tcttgaagag cttttagata gctgttttcc accccagact
     2701 agaagagtta ttctacagtg ctactgtcct cctataaggg accgtcagaa attgttccca
     2761 cagtggttgc ttccacaaat gtggagttgg atttttgatc tattgttgtg attcaagcag
     2821 tctgttgaat taaaatccag ggtttgaccg ctcaacaagt agtaaattac ctagtaactc
     2881 agggacaact atttctgaac ttataagtgg cactgtaata tacttattta cctttggata
     2941 accttgtgtt tctttgggct agatcaagga ctactgggaa ggtaggagtg gttgcctttc
     3001 tcctgaagct ctttcccttc gtttccctca tggtgccctg accatttgct acacatgaaa
     3061 cgagcctaaa tattagggct tgcttttctg tcagaggtgt catctgtgcc tttcctagtg
     3121 ttcatctgac aatgcctcta catttgacat aaactcatac tcaggtggca ctaaaccagg
     3181 aggcatttct cccagtaata catcatcatt agtgttaggc aattatgtta tagaaaaggt
     3241 cccatcacta ccccaaaatc ctgccaccta tgtccaccca tcagctactc ccacagaatt
     3301 ttttagatta tagaattgtt ggctgttaac taatttgctc aaagttcttg tagaggattg
     3361 caaattttag tctgtttttg taatgggata atattgatgg ctttatgacc tcagagttta
     3421 aaattcacat ggaattgatt ttcttgtcat tagaaggtaa tgcttgaaca tttgattttt
     3481 ctattctgtg atacctgggt gtcatactgt ctaatggcct ttggtcccaa ttgtgaaatt
     3541 ttatatgagc tcattcattc tctacaaacc tgcccccttt gggtcttggt gcctatgtat
     3601 cattgtctgg tctctagttg gtggaattgc ccttttttgt actgccactt ctcaacattt
     3661 ttgaagtttg acataatgtt gcttcattcc agtttttttt ttttttagtt ctgtaatttg
     3721 ttgactgtat ttaactatgt gagctttttt ttttctgtga tgtttactgt attgtaaagc
     3781 accttaatca tgtgatgggt gctctataaa tcaataaatg atgacttaga ggctgta
//
