LOCUS       NM_026454               1892 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2F (putative) (Ube2f),
            transcript variant 1, mRNA.
ACCESSION   NM_026454
VERSION     NM_026454.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Zhang,F., Xiong,X., Li,Z., Wang,H., Wang,W., Zhao,Y. and Sun,Y.
  TITLE     RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and
            aggravates liver tumorigenesis
  JOURNAL   EMBO J 44 (4), 1185-1219 (2025)
   PUBMED   39762645
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Chang,Y., Chen,Q., Li,H., Xu,J., Tan,M., Xiong,X. and Sun,Y.
  TITLE     The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor
            cascade in pancreatic cancer cells
  JOURNAL   Dev Cell 59 (10), 1317-1332 (2024)
   PUBMED   38574733
REFERENCE   3  (bases 1 to 1892)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1892)
  AUTHORS   Wu,D., Li,H., Liu,M., Qin,J. and Sun,Y.
  TITLE     The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are
            essential for the maintenance of Regulatory T cell fitness
  JOURNAL   Nat Commun 13 (1), 3021 (2022)
   PUBMED   35641500
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1892)
  AUTHORS   Huang,D.T., Ayrault,O., Hunt,H.W., Taherbhoy,A.M., Duda,D.M.,
            Scott,D.C., Borg,L.A., Neale,G., Murray,P.J., Roussel,M.F. and
            Schulman,B.A.
  TITLE     E2-RING expansion of the NEDD8 cascade confers specificity to
            cullin modification
  JOURNAL   Mol Cell 33 (4), 483-495 (2009)
   PUBMED   19250909
REFERENCE   6  (bases 1 to 1892)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB621519.1, AK153481.1, AK030594.1 and BQ032120.1.
            
            On Sep 27, 2017 this sequence version replaced NM_026454.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.210779.1,
                                           SRR1660817.303522.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BB621519.1         1-15
            16-1378             AK153481.1         2-1364
            1379-1594           AK030594.1         1344-1559
            1595-1892           BQ032120.1         1-298               c
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 46.08 cM"
     gene            1..1892
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="ubiquitin-conjugating enzyme E2F (putative)"
                     /db_xref="GeneID:67921"
                     /db_xref="MGI:MGI:1915171"
     exon            1..134
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            135..268
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..708
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /EC_number="2.3.2.34"
                     /note="NEDD8 protein ligase UBE2F; NEDD8-conjugating
                     enzyme 2; NEDD8 carrier protein UBE2F;
                     ubiquitin-conjugating enzyme E2 F; RING-type E3 NEDD8
                     transferase UBE2F"
                     /codon_start=1
                     /product="NEDD8-conjugating enzyme UBE2F"
                     /protein_id="NP_080730.1"
                     /db_xref="CCDS:CCDS48322.1"
                     /db_xref="GeneID:67921"
                     /db_xref="MGI:MGI:1915171"
                     /translation="MLTLASKLKRDDGLKGSRTSASTSDSTRRVSVRDKLLVKEVAEL
                     EANLPCTCKVHFPDPNKLHCFQLTVSPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLT
                     KIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIE
                     AAEHHLRDKEDFRDKVDEYIKRYAR"
     exon            269..298
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            299..364
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            365..432
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            433..503
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            504..561
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            562..594
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            595..657
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            658..1874
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1116..1121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="hexamer: TATAAA"
     polyA_site      1136
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="major polyA site"
     polyA_site      1311
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="major polyA site"
     regulatory      1848..1853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1874
                     /gene="Ube2f"
                     /gene_synonym="2510010F15Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtccccgcc ccgccacttc cggtctcgcc gccgggagcc ggtgcggctg tgaaggccag
       61 cgcgactcag tgccgctgct ccgccgggca tggttttggg tgccggctcg cctcgcctga
      121 ctcgcggtgc tcagaagaaa ggtggcagga atgctaacgc tggcaagcaa gttgaagcgg
      181 gatgatggtc tcaaaggatc ccggacatca gcctccacat ctgactctac ccggagggtt
      241 tctgtgagag acaagttgct tgttaaagag gttgcagaac ttgaagctaa tttaccttgt
      301 acatgtaaag tacattttcc tgatccaaac aagcttcatt gctttcagct gactgtaagc
      361 ccagatgagg gttactacca gggtggaaaa tttcagtttg aaactgaagt tcccgatgcc
      421 tacaacatgg tgcctcccaa ggtgaaatgc ttgactaaaa tctggcaccc caacatcaca
      481 gaaacggggg aaatatgtct aagtttacta agagaacatt caattgatgg cactggctgg
      541 gctcccacta gaacattaaa ggatgttgtt tggggattaa actctttatt taccgatctc
      601 ttgaattttg atgatccact gaatattgaa gctgcagaac atcatttgcg ggacaaggag
      661 gattttcggg acaaagtgga tgaatacatc aaacgctatg ccagatgata agaggagaga
      721 tcgcaggtct gaggactttg tcacagttgt ctctgatgtg aaacagctcg aggtagccct
      781 cctccccatc ctcatactcc ctgtcagccc cttgggattg tcccagtcct gcgaccatgc
      841 tgtcctgaag aagaccctcc tgactgccca ctataggtgg cgaacaacat aatacagcaa
      901 gaaagtgtgt ctgggcctca agagagttgt ctgcttccct taacatgttt actgaaacta
      961 ctgtctaggc tgtctggtgg aatccctctg aagttgtaat gaactcacca ctgaggatat
     1021 agcgcttgct tcctttcccc caacaatcgg tgtcctgcac aagtggtgta atgacatgtt
     1081 ccatgtgact ggcagattgg caataagaaa cccgctataa actgtgattg gatgcacact
     1141 ctcttagctt cttccacgaa tgttgaccct acctagatgt gaagcttccc agaatgcata
     1201 gtcattcact gtagatctta ctgaaatgcg tattttattt aatgtaagta tattttggaa
     1261 cagatttgta atttgtacaa tttgatgctt taattatttt ttctattctt atttactttg
     1321 tattcattgt atagagcaaa cacaaagaca ttgagtcaag aaactactga agagaaatac
     1381 aaagagacca tgctaggcac ataattcagt ttctccagac gcagcagtag tctggctcct
     1441 agacagcagc tcttccttca ggtctacatg tggatgtctt agagccttag aacctacacc
     1501 tggacgatca gaaccaaagg cagggaggct tccctcgatt gctacccaga cacagcaggc
     1561 tagggctctc taagcaaggc tggtaggtga ttatgccatg gtcatagcat gagccagggc
     1621 tccttaacag actctgtcat tgccatcctg cctggggagt cagcccagtc taagactctg
     1681 ccacaactcc attatgactg tcttccaatt tgccagtggt tccaagaggg ggagtttcat
     1741 agctgcagca tacgccataa tcagggtagt cacttcaggg tcagccctgg agagacaggt
     1801 gggcttattc tggctttaga catctgaacc agtgaaatct aatgcaaaat aaatgcatgt
     1861 ctggttttac tcacaaaaaa aaaaaaaaaa aa
//
