LOCUS       NM_144839               1545 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2E 2 (Ube2e2),
            transcript variant 1, mRNA.
ACCESSION   NM_144839
VERSION     NM_144839.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Sakurai,Y., Kubota,N., Takamoto,I., Wada,N., Aihara,M., Hayashi,T.,
            Kubota,T., Hiraike,Y., Sasako,T., Nakao,H., Aiba,A., Chikaoka,Y.,
            Kawamura,T., Kadowaki,T. and Yamauchi,T.
  TITLE     Overexpression of UBE2E2 in Mouse Pancreatic beta-Cells Leads to
            Glucose Intolerance via Reduction of beta-Cell Mass
  JOURNAL   Diabetes 73 (3), 474-489 (2024)
   PUBMED   38064504
  REMARK    GeneRIF: Overexpression of UBE2E2 in Mouse Pancreatic beta-Cells
            Leads to Glucose Intolerance via Reduction of beta-Cell Mass.
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1545)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1545)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1545)
  AUTHORS   West,D.B., Engelhard,E.K., Adkisson,M., Nava,A.J., Kirov,J.V.,
            Cipollone,A., Willis,B., Rapp,J., de Jong,P.J. and Lloyd,K.C.
  TITLE     Transcriptome Analysis of Targeted Mouse Mutations Reveals the
            Topography of Local Changes in Gene Expression
  JOURNAL   PLoS Genet 12 (2), e1005691 (2016)
   PUBMED   26839965
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1545)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118006.9, BY752670.1, DN172861.1, CF732742.1 and AW495303.1.
            
            On Jan 31, 2018 this sequence version replaced NM_144839.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016265.1, BI415484.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC118006.9         147434-147511
            79-117              BY752670.1         1-39
            118-569             DN172861.1         1-452
            570-960             CF732742.1         67-457
            961-1545            AW495303.1         1-585               c
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 7.08 cM"
     gene            1..1545
                     /gene="Ube2e2"
                     /note="ubiquitin-conjugating enzyme E2E 2"
                     /db_xref="GeneID:218793"
                     /db_xref="MGI:MGI:2384997"
     exon            1..187
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            188..371
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..801
                     /gene="Ube2e2"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1; TBC1
                     domain family, member 12; ubiquitin-conjugating enzyme E2E
                     2 (UBC4/5 homolog, yeast); ubiquitin-protein ligase E2;
                     ubiquitin carrier protein E2; E2 ubiquitin-conjugating
                     enzyme E2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 E2 isoform 1"
                     /protein_id="NP_659088.1"
                     /db_xref="CCDS:CCDS26838.1"
                     /db_xref="GeneID:218793"
                     /db_xref="MGI:MGI:2384997"
                     /translation="MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPDREQVQPKKKEGK
                     ISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGG
                     VFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSI
                     CSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT"
     exon            372..422
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            423..555
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            556..703
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..1529
                     /gene="Ube2e2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1495..1500
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2e2"
                     /note="hexamer: AATAAA"
     polyA_site      1517
                     /gene="Ube2e2"
                     /note="major polyA site"
ORIGIN      
        1 gaggccgacg tggggctcgc gctgtcgcgg gctctcaggg caggcagggc gagcgcgcgc
       61 ttgggagggg gcgcgcgcgg acggcgggcg gcggcggcgg tggcggcggc ggcggctcgc
      121 ggaggcggcg gtttcactcc cggactcggc gtcggagccg cggctggagc gcgggcgacg
      181 ccgcgaggat ctaagatgtc cactgaggcg cagagagttg atgacagtcc aagcactagt
      241 ggaggaagtt ctgatggaga tcagcgggag agcgttcagc aggaacctga tcgagaacaa
      301 gttcagccta agaaaaagga ggggaagata tccagcaaga cagccgcgaa actgtcaacc
      361 agtgccaaaa gaattcagaa ggagcttgca gaaatcacgc tggatccccc tcccaactgt
      421 agtgctggac ccaaaggaga caacatttat gaatggaggt caactatatt gggaccccca
      481 ggatctgtct atgaaggagg ggtattcttt cttgacatta ccttttcacc agattatccg
      541 tttaaacccc ctaaggtcac cttccgaacg agaatctatc actgtaatat caacagccag
      601 ggtgtaatct gtctggacat cctcaaggac aactggagtc cggccctgac catctctaaa
      661 gtcctcctgt ccatctgctc actacttaca gactgcaacc ctgccgaccc tctggtgggc
      721 agtattgcca cacagtacat gaccaacaga gcggagcatg accggatggc cagacagtgg
      781 accaagcggt acgccaccta ggggcctgcg tgtgccagct gtgtgccagc acattcacca
      841 agtgcatcag tacccacctg ctcctgcagc cctccgttct gtcgcctgtt gttattacat
      901 tttaaactgt ttggggatgg tatgtcatct gtccctcctc cttcctaccg ccacctcctc
      961 cccttctctc tgtccctctt tttcctttta ttccattttc ggtttctgtt aacttgaaag
     1021 atttgggatt cccccactgc agtgtggatg ggagtttctg ttttaagtac aaaccatgtt
     1081 ggagctatac tagtaagacc tttcatacag agctttgtgt tactgtgtgg tttgggtttt
     1141 tgttgttgtt gtttgtttta tctttttttt ttcttttatg tgctcttcgg ggaaaacaaa
     1201 cattcaggag tatatctcgt ttatacttaa atgtagtgct tagggttaat tttttgtact
     1261 gaagtctatt ggtgggtgca tgctactggg aacaaggttt tgtacaaaag gtccaatcag
     1321 aatcactgtg cattactgag accctgtcac aagccttctg tcccccacac agaagactgt
     1381 cagattgaac aaaataatat gtattttgat ttacttaagt gcttgtaaat ttcttaggga
     1441 cctgccactt ttgactgtgg atcagttgat gtatacttgt attattaatg cctcaataaa
     1501 tcactgtggc ggatcaatgc ccttctgtaa aaaaaaaaaa aaaaa
//
