LOCUS       NM_019912               2483 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2D 2A (Ube2d2a), mRNA.
ACCESSION   NM_019912
VERSION     NM_019912.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   McKelvey,A.C., Lear,T.B., Dunn,S.R., Evankovich,J., Londino,J.D.,
            Bednash,J.S., Zhang,Y., McVerry,B.J., Liu,Y. and Chen,B.B.
  TITLE     RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate
            immunity
  JOURNAL   Elife 5, e18496 (2016)
   PUBMED   27805901
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Starita,L.M., Pruneda,J.N., Lo,R.S., Fowler,D.M., Kim,H.J.,
            Hiatt,J.B., Shendure,J., Brzovic,P.S., Fields,S. and Klevit,R.E.
  TITLE     Activity-enhancing mutations in an E3 ubiquitin ligase identified
            by high-throughput mutagenesis
  JOURNAL   Proc Natl Acad Sci U S A 110 (14), E1263-E1272 (2013)
   PUBMED   23509263
REFERENCE   3  (bases 1 to 2483)
  AUTHORS   Magiera,M.M., Mora,S., Mojsa,B., Robbins,I., Lassot,I. and
            Desagher,S.
  TITLE     Trim17-mediated ubiquitination and degradation of Mcl-1 initiate
            apoptosis in neurons
  JOURNAL   Cell Death Differ 20 (2), 281-292 (2013)
   PUBMED   22976837
REFERENCE   4  (bases 1 to 2483)
  AUTHORS   Hoxhaj,G., Najafov,A., Toth,R., Campbell,D.G., Prescott,A.R. and
            MacKintosh,C.
  TITLE     ZNRF2 is released from membranes by growth factors and, together
            with ZNRF1, regulates the Na+/K+ATPase
  JOURNAL   J Cell Sci 125 (Pt 19), 4662-4675 (2012)
   PUBMED   22797923
  REMARK    Erratum:[J Cell Sci. 2022 Mar 15;135(6):jcs259936. doi:
            10.1242/jcs.259936.. PMID: 35267011]
REFERENCE   5  (bases 1 to 2483)
  AUTHORS   Campos,Y., Qiu,X., Zanoteli,E., Moshiach,S., Vergani,N.,
            Bongiovanni,A., Harris,A.J. and d'Azzo,A.
  TITLE     Ozz-E3 ubiquitin ligase targets sarcomeric embryonic myosin heavy
            chain during muscle development
  JOURNAL   PLoS One 5 (3), e9866 (2010)
   PUBMED   20352047
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2483)
  AUTHORS   Lu,Z., Je,H.S., Young,P., Gross,J., Lu,B. and Feng,G.
  TITLE     Regulation of synaptic growth and maturation by a
            synapse-associated E3 ubiquitin ligase at the neuromuscular
            junction
  JOURNAL   J Cell Biol 177 (6), 1077-1089 (2007)
   PUBMED   17576800
REFERENCE   7  (bases 1 to 2483)
  AUTHORS   Bedard,N., Hingamp,P., Pang,Z., Karaplis,A., Morales,C.,
            Trasler,J., Cyr,D., Gagnon,C. and Wing,S.S.
  TITLE     Mice lacking the UBC4-testis gene have a delay in postnatal testis
            development but normal spermatogenesis and fertility
  JOURNAL   Mol Cell Biol 25 (15), 6346-6354 (2005)
   PUBMED   16024774
REFERENCE   8  (bases 1 to 2483)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2483)
  AUTHORS   Lorick,K.L., Jensen,J.P., Fang,S., Ong,A.M., Hatakeyama,S. and
            Weissman,A.M.
  TITLE     RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent
            ubiquitination
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11364-11369 (1999)
   PUBMED   10500182
REFERENCE   10 (bases 1 to 2483)
  AUTHORS   Hatakeyama,S., Jensen,J.P. and Weissman,A.M.
  TITLE     Subcellular localization and ubiquitin-conjugating enzyme (E2)
            interactions of mammalian HECT family ubiquitin protein ligases
  JOURNAL   J Biol Chem 272 (24), 15085-15092 (1997)
   PUBMED   9182527
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY136374.1, AK148089.1, AK147568.1 and BE688817.1.
            
            On Aug 14, 2009 this sequence version replaced NM_019912.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U62483.1, AK136197.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-179               BY136374.1         1-179
            180-971             AK148089.1         2-793
            972-2472            AK147568.1         6658-8158
            2473-2483           BE688817.1         3-13                c
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 19.24 cM"
     gene            1..2483
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /note="ubiquitin-conjugating enzyme E2D 2A"
                     /db_xref="GeneID:56550"
                     /db_xref="MGI:MGI:1930715"
     exon            1..454
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     CDS             431..874
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="ubiquitin conjugating enzyme 2e; E2(17)KB 2;
                     ubiquitin-protein ligase D2; ubiquitin carrier protein D2;
                     ubiquitin-conjugating enzyme E2-17 kDa 2;
                     ubiquitin-conjugating enzyme E2(17)KB 2;
                     ubiquitin-conjugating enzyme E2D 2; E2
                     ubiquitin-conjugating enzyme D2; (E3-independent) E2
                     ubiquitin-conjugating enzyme D2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 D2"
                     /protein_id="NP_064296.1"
                     /db_xref="CCDS:CCDS50254.1"
                     /db_xref="GeneID:56550"
                     /db_xref="MGI:MGI:1930715"
                     /translation="MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSP
                     YQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKV
                     LLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM"
     exon            455..518
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     exon            519..550
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     exon            551..628
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     exon            629..734
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     exon            735..828
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     exon            829..2483
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1717..1722
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /note="hexamer: AATAAA"
     polyA_site      1748
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /note="major polyA site"
     regulatory      2447..2452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /note="hexamer: AATAAA"
     polyA_site      2475
                     /gene="Ube2d2a"
                     /gene_synonym="1500034D03Rik; Ubc2e; ubc4; Ube2d2"
                     /note="major polyA site"
ORIGIN      
        1 ggcggcgccg cccgcccgcg cgtcccttag tccacctgca ccaggcagga gagcaggctc
       61 cccccggctc cgccaccgta aggcgctggg cgagcccgga gcttgggggc tcttcctccc
      121 aggcggaggg atccacggcg gccgaggagg cggcgctgag cgtacgagga agaggcagct
      181 acggcggcgg cggcggctgt ggcagcagcg cataaagggg ccgccgccgg gtgatgcggt
      241 gaccgctgcg gcaggcccag aagctgagtg gggcctcggc cctcagctcg tctgatccgc
      301 actcccggga gctcccggtc ttctccggtc gactcgcgac cgctcgggcg gtctcaggcc
      361 ccctcgcccc ttccccgtcc ctcccccgtc cccgtccccg ccccgggggc cgtcgccacc
      421 gtttcccacc atggctctga agagaatcca caaggaattg aatgacctgg ctcgagatcc
      481 cccagcacag tgttcagcag gtcctgttgg agatgatatg tttcattggc aggctacaat
      541 aatggggcca aatgacagcc cctatcaggg tggagtattt ttcttgacaa ttcatttccc
      601 aacagattac cccttcaaac cgcctaaggt tgcatttaca acaagaattt atcacccaaa
      661 tattaacagt aatggcagca tttgtcttga tattctacgg tcacagtggt ctccagcact
      721 aactatttca aaagtacttt tgtccatctg ttctctgttg tgtgatccca atccagatga
      781 tcctttagtg cctgagattg ctcggatcta caaaacagat agagaaaagt acaacagaat
      841 agctcgggaa tggactcaga agtatgcgat gtaattaaag aaattattgg ataacctcta
      901 caaataaagc taggggaact ctgagagaga gaaagtcctt ttgatttcca tttgactgct
      961 ttctatgagc ccacgcctca tcttcccctg tgcacatgtt tacctgacac agcagtgctg
     1021 cgtgttgtac atacttggaa caacaaacta gaaatactgt acttctgtac caacattgcc
     1081 tcctagcaga gaagtgtgtg tgtgagaagc cagttctccg ggcataacct aggtgtgaga
     1141 ctaaaagctt tccttattga cttaactttg gataatggca agatgtgagg ggtagtaggt
     1201 acacagtgtg tgcttggatg ggaggaaaag ctccagtgac ccataggaga ttgtccttaa
     1261 gtggagtcca tccaatctca aacagtcata aaaagcaagg tgaagaacgt gaagctgttt
     1321 ctatattcat ttcatcctga aggacctaca acttaggtga aagttatgac caaacagatt
     1381 aaactacatg ctgtgcttta aggtctaagg ttaacggatc aacatttaaa tggactggag
     1441 ctgttagtac ataaagtgtg gtgggctttg ttcccaacac ttcacatctc cctgcccttt
     1501 cagccttcct ccttttagcc cctttccata atctttggtt tgtatgtggt ttctcagtta
     1561 atacatagct aatagctctt atttttctta tgtttttaac tgctttaggc ctatttcgat
     1621 gtaagggtgg agatgtattt gatggaaatc cgtgtgtata tttaagaccc tgttgctcct
     1681 ctggagcttg tacgttcacg aatgattcat ctgtgtaata aactgattac tacagtcatt
     1741 acagataatt ttgtgtgaat aggctttttg atttaaaaac ttggtctagc taagattagt
     1801 ggtggatttt tcctcacccc tgaaatgttt tcatctatat ttgttgcatt tcaaaatcat
     1861 gaaacaattc cagtttacat actaaaaaag atataaaagt aattttatta aacaaggtag
     1921 aggcataagc ttaaaggcat tttcatgaaa cttatatgtg caccattcca ggaacatgac
     1981 tgttaaacta aataaatttg ctttgttaat gaaactgagc agcatttcac caaaaccttg
     2041 tgacagtctc ttggtacata gggcatagaa tgcaaaggtg ttggttgctg tttgttaagt
     2101 taattgattg taatcataaa gagtagctga ccgaacctgg gaaacatgag cacaatcttg
     2161 tattggagag tctgtgttgt tactttgcac taacatttgc aggatgtccc ttggttttaa
     2221 attgtactgt atgtggcttt ttgaagtctt cccttgactc tagtaaaatg tagtttgaaa
     2281 tttgtaaaca actgttaatc cagaaacatt catgtgaact aggctagtta cctcccctcg
     2341 tcccctttcc taacctaaat gtaagccagg ccagcttgaa ggccaatgct ctttagtcac
     2401 caagatcttt gaaatgcatc tttacactct tgcacaaaaa tcgaggaata aatgaccact
     2461 gcttttggtt ttaaacttgt tca
//
