LOCUS       NM_009458               2158 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2B (Ube2b), transcript
            variant 2, mRNA.
ACCESSION   NM_009458
VERSION     NM_009458.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Ye,Q., Wang,J., Liu,X., Liu,Z., BaZong,L., Ma,J., Shen,R., Ye,W.,
            Zhang,W. and Wang,D.
  TITLE     The Role of RAD6B and PEDF in Retinal Degeneration
  JOURNAL   Neuroscience 480, 19-31 (2022)
   PUBMED   34774969
  REMARK    GeneRIF: The Role of RAD6B and PEDF in Retinal Degeneration.
REFERENCE   3  (bases 1 to 2158)
  AUTHORS   Ma,Y., Song,Y., Shen,R., Li,P., Ding,H., Guo,Z., Liu,X. and Wang,D.
  TITLE     Loss of RAD6B induces degeneration of the cochlea in mice
  JOURNAL   Biochem Biophys Res Commun 531 (3), 402-408 (2020)
   PUBMED   32868078
  REMARK    GeneRIF: Loss of RAD6B induces degeneration of the cochlea in mice.
REFERENCE   4  (bases 1 to 2158)
  AUTHORS   Guo,Z., Tian,Y., Guo,Y., Li,B., Liu,X., Xie,K., Song,Y. and Wang,D.
  TITLE     RAD6B Plays a Critical Role in Neuronal DNA Damage Response to
            Resist Neurodegeneration
  JOURNAL   Front Cell Neurosci 13, 392 (2019)
   PUBMED   31507381
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2158)
  AUTHORS   Zhou,J., Wang,R., Zhang,J., Zhu,L., Liu,W., Lu,S., Chen,P., Li,H.,
            Yin,B., Yuan,J., Qiang,B., Shu,P. and Peng,X.
  TITLE     Conserved expression of ultra-conserved noncoding RNA in mammalian
            nervous system
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1860 (12), 1159-1168 (2017)
   PUBMED   29055695
REFERENCE   6  (bases 1 to 2158)
  AUTHORS   Roest,H.P., van Klaveren,J., de Wit,J., van Gurp,C.G., Koken,M.H.,
            Vermey,M., van Roijen,J.H., Hoogerbrugge,J.W., Vreeburg,J.T.,
            Baarends,W.M., Bootsma,D., Grootegoed,J.A. and Hoeijmakers,J.H.
  TITLE     Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in
            mice causes male sterility associated with chromatin modification
  JOURNAL   Cell 86 (5), 799-810 (1996)
   PUBMED   8797826
REFERENCE   7  (bases 1 to 2158)
  AUTHORS   Wefes,I., Kaiser,P., Schneider,R., Pickart,C.M. and Finley,D.
  TITLE     Characterization of a cDNA clone encoding E2-20K, a murine
            ubiquitin-conjugating enzyme
  JOURNAL   Gene 163 (2), 321-322 (1995)
   PUBMED   7590289
REFERENCE   8  (bases 1 to 2158)
  AUTHORS   Roller,M.L., Lossie,A.C., Koken,M.H., Smit,E.M., Hagemeijer,A. and
            Camper,S.A.
  TITLE     Localization of sequences related to the human RAD6 DNA repair gene
            on mouse chromosomes 11 and 13
  JOURNAL   Mamm Genome 6 (4), 305-306 (1995)
   PUBMED   7613042
REFERENCE   9  (bases 1 to 2158)
  AUTHORS   Koken,M.H., Smit,E.M., Jaspers-Dekker,I., Oostra,B.A.,
            Hagemeijer,A., Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Localization of two human homologs, HHR6A and HHR6B, of the yeast
            DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31
  JOURNAL   Genomics 12 (3), 447-453 (1992)
   PUBMED   1559696
REFERENCE   10 (bases 1 to 2158)
  AUTHORS   Chau,V., Tobias,J.W., Bachmair,A., Marriott,D., Ecker,D.J.,
            Gonda,D.K. and Varshavsky,A.
  TITLE     A multiubiquitin chain is confined to specific lysine in a targeted
            short-lived protein
  JOURNAL   Science 243 (4898), 1576-1583 (1989)
   PUBMED   2538923
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669920.15, AK147785.1, AK169229.1 and BE944184.1.
            
            On Apr 25, 2018 this sequence version replaced NM_009458.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147785.1, AK169229.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AL669920.15        166179-166203       c
            26-43               AK147785.1         2-19
            44-1738             AK169229.1         2-1696
            1739-2150           BE944184.1         17-428              c
            2151-2158           AL669920.15        150850-150857       c
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 31.82 cM"
     gene            1..2158
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /note="ubiquitin-conjugating enzyme E2B"
                     /db_xref="GeneID:22210"
                     /db_xref="MGI:MGI:102944"
     exon            1..199
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..614
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 2;
                     ubiquitin-protein ligase B; ubiquitin carrier protein B;
                     RAD6 homolog B; ubiquitin-conjugating enzyme E2B, RAD6
                     homology; E2 ubiquitin-conjugating enzyme B"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 B isoform 1"
                     /protein_id="NP_033484.3"
                     /db_xref="CCDS:CCDS24664.1"
                     /db_xref="GeneID:22210"
                     /db_xref="MGI:MGI:102944"
                     /translation="MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPE
                     GTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDV
                     SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS"
     exon            200..280
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     exon            281..306
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     exon            307..396
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     exon            397..485
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     exon            486..2158
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1019..1024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /note="hexamer: AATACA"
     polyA_site      1038
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /note="major polyA site"
     regulatory      1721..1726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /note="hexamer: AGTAAA"
     polyA_site      1741
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
     regulatory      2124..2129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
                     /note="hexamer: AATAAA"
     polyA_site      2150
                     /gene="Ube2b"
                     /gene_synonym="E2-14k; E214K; HR6B; mHR6B; Rad6b"
ORIGIN      
        1 ggatctggag gcggcggagg gagacgtcat tgcagggttg tttgtgcagt ctcggtggcg
       61 gcggcgaccc acagtgattc ggccgctgcg ccggggggtg ggggggctga acgggactct
      121 tttctttcag actgaccgcg gggcagctgc ggagcatgtc gaccccggcc cgtaggaggc
      181 tcatgcggga tttcaagcga ttgcaagagg acccacctgt gggggtcagt ggcgccccat
      241 ctgaaaacaa catcatgcag tggaatgcag ttatatttgg accagaaggg acaccctttg
      301 aagatggtac ttttaaacta gtaatagaat tttctgaaga atatccaaat aaaccaccaa
      361 ccgttaggtt tttatccaaa atgtttcatc caaatgtgta tgctgatggt agcatatgtt
      421 tagatatcct gcagaatcga tggagtccca catatgatgt ctcttccatc ttaacttcca
      481 ttcagtctct gctggatgaa ccgaatccaa acagtccagc caacagccaa gcagcacagc
      541 tttatcagga aaacaaacgg gagtatgaga agagagtttc ggccattgtt gagcaaagct
      601 ggaatgattc ataatagaca cctggtctgc tcatccttca tcgttgttgt gtataattta
      661 cctcccagta gaaaggctag caagttttaa gtgccacggg ttttaaggat tctgcagaga
      721 aaaaaagaaa aagtccttca gtttagaacc tacaaaagct tgtgtatctt gattaatgta
      781 ctttttattg catggtgtga actaagttat tgctgcataa atttgtaata tatcctgttt
      841 gtattttttt ccaagtgtat aatgttggtg tggagttttc atgacagaat atacacattt
      901 tgtaaatctg tacttttttc aaatattgaa tgccttattt ttgaattctt tagattttta
      961 aattggagaa aagcacttaa agttttttat atatgaatat tacgtgtaaa gctgttaaaa
     1021 tacataactt cagtgcaaga gactttgtca cttatttcct cacctgtgca agagggactg
     1081 cgaagtctct gccaggtgag agcctcattt ccaactgcag aaggtcactc ttctgtttat
     1141 caagaaagtc tgcaaattcg attctaaaac caattctgat ctccggcttt attttgaatg
     1201 tacttctgta agttcagttg agtaactata gacttactgg tctggttctg tccagctctg
     1261 tatttgggcc acttgttaat gtgcgtttct catgtaaact gtaccttttg tgacttcaca
     1321 attggcacct ccaccacgag cagagctgac ttcctctgct gtgtggcagc accttcacag
     1381 gcttttgatt aaaaagaaag taaaaccatc accatttacc aaacaccgtg ccgcagtatt
     1441 agcaatggcc ggttgcgctg gtttgagcaa ggcagagatt tggaaggaat cttggtgtaa
     1501 cttgaatatt tgcaaactac cttttaatgc agctgcgcgc tacctgttta ttctggaact
     1561 gttgagcacc agggcctgcc gagttgcttc tcacctcctc agttgtatcc aagttgtact
     1621 gcatcttagt ttactggata gatttaaaac acagtattgt agaaagctaa tacaaagcta
     1681 tcctatgcct tcaaatagta tagaaaatgg aaaatataca agtaaattct gttgaaccac
     1741 ttgtgcagtc tctctagtag ttgaaacagc aattgcagtg agcctgagtt tcttgtcatc
     1801 cacaccagcc tcctagagat gtgctctggt agcctctctg ctggaacgtt gggttgcaga
     1861 gtgaccatgt cttctgttta caagcaaagt tgaaaactgg gatggataag aactgatact
     1921 tccctttaga agtcgttact ccagagggct gaaaagatgg ttcagtggtt actgcatgta
     1981 ctgtttctgc aagaacccag tctctgttcc cagctctcac ttcagacagt tcccagctgc
     2041 ctgtaacttc agctcctggg gttccaaagc ttctgactaa atcagacact gacagtcaca
     2101 caaacagacg cttacataat taaaataaaa atactaattt attaagctta gcatggtg
//
