LOCUS       NM_019668               1746 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript
            variant 1, mRNA.
ACCESSION   NM_019668
VERSION     NM_019668.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Hildebrandt,M.R., Wang,Y., Li,L., Yasmin,L., Glubrecht,D.D. and
            Godbout,R.
  TITLE     Cytoplasmic aggregation of DDX1 in developing embryos: Early
            embryonic lethality associated with Ddx1 knockout
  JOURNAL   Dev Biol 455 (2), 420-433 (2019)
   PUBMED   31330130
REFERENCE   3  (bases 1 to 1746)
  AUTHORS   Cao,Y., Li,M., Liu,F., Ni,X., Wang,S., Zhang,H., Sui,X. and Huo,R.
  TITLE     Deletion of maternal UHRF1 severely reduces mouse oocyte quality
            and causes developmental defects in preimplantation embryos
  JOURNAL   FASEB J 33 (7), 8294-8305 (2019)
   PUBMED   30995416
REFERENCE   4  (bases 1 to 1746)
  AUTHORS   Cui,W., Dai,X., Marcho,C., Han,Z., Zhang,K., Tremblay,K.D. and
            Mager,J.
  TITLE     Towards Functional Annotation of the Preimplantation Transcriptome:
            An RNAi Screen in Mammalian Embryos
  JOURNAL   Sci Rep 6, 37396 (2016)
   PUBMED   27869233
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1746)
  AUTHORS   Bruinsma,C.F., Savelberg,S.M., Kool,M.J., Jolfaei,M.A., Van
            Woerden,G.M., Baarends,W.M. and Elgersma,Y.
  TITLE     An essential role for UBE2A/HR6A in learning and memory and
            mGLUR-dependent long-term depression
  JOURNAL   Hum Mol Genet 25 (1), 1-8 (2016)
   PUBMED   26476408
  REMARK    GeneRIF: results indicate a causal role of UBE2A in learning and
            mGLUR-dependent long-term depression
REFERENCE   6  (bases 1 to 1746)
  AUTHORS   Tateishi,S., Niwa,H., Miyazaki,J., Fujimoto,S., Inoue,H. and
            Yamaizumi,M.
  TITLE     Enhanced genomic instability and defective postreplication repair
            in RAD18 knockout mouse embryonic stem cells
  JOURNAL   Mol Cell Biol 23 (2), 474-481 (2003)
   PUBMED   12509447
REFERENCE   7  (bases 1 to 1746)
  AUTHORS   Engel,I. and Murre,C.
  TITLE     Disruption of pre-TCR expression accelerates lymphomagenesis in
            E2A-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 99 (17), 11322-11327 (2002)
   PUBMED   12172006
  REMARK    GeneRIF: Disruption of pre-TCR expression accelerates
            lymphomagenesis in E2A-deficient mice.
REFERENCE   8  (bases 1 to 1746)
  AUTHORS   Kwon,Y.T., Xia,Z., Davydov,I.V., Lecker,S.H. and Varshavsky,A.
  TITLE     Construction and analysis of mouse strains lacking the ubiquitin
            ligase UBR1 (E3alpha) of the N-end rule pathway
  JOURNAL   Mol Cell Biol 21 (23), 8007-8021 (2001)
   PUBMED   11689692
REFERENCE   9  (bases 1 to 1746)
  AUTHORS   Grootegoed,J.A., Baarends,W.M., Roest,H.P. and Hoeijmakers,J.H.
  TITLE     Knockout mouse model and gametogenic failure
  JOURNAL   Mol Cell Endocrinol 145 (1-2), 161-166 (1998)
   PUBMED   9922113
  REMARK    Review article
REFERENCE   10 (bases 1 to 1746)
  AUTHORS   Koken,M.H., Smit,E.M., Jaspers-Dekker,I., Oostra,B.A.,
            Hagemeijer,A., Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Localization of two human homologs, HHR6A and HHR6B, of the yeast
            DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31
  JOURNAL   Genomics 12 (3), 447-453 (1992)
   PUBMED   1559696
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA013850.1, AK164074.1 and BE994531.1.
            
            On Aug 27, 2015 this sequence version replaced NM_019668.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026053.1, AK133723.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AA013850.1         1-63
            64-1726             AK164074.1         2-1664
            1727-1746           BE994531.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 21.28 cM"
     gene            1..1746
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /note="ubiquitin-conjugating enzyme E2A"
                     /db_xref="GeneID:22209"
                     /db_xref="MGI:MGI:102959"
     exon            1..235
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..650
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme E2A (RAD6 homolog);
                     ubiquitin-conjugating enzyme HR6A; ubiquitin-protein
                     ligase A; ubiquitin carrier protein A; RAD6 homolog A; E2
                     ubiquitin-conjugating enzyme A"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 A isoform 1"
                     /protein_id="NP_062642.1"
                     /db_xref="CCDS:CCDS30064.1"
                     /db_xref="GeneID:22209"
                     /db_xref="MGI:MGI:102959"
                     /translation="MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPE
                     GTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDV
                     SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC"
     exon            236..316
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     exon            317..342
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     exon            343..432
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     exon            433..521
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     exon            522..1732
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      741..746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /note="hexamer: ATTAAA"
     polyA_site      753
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /note="major polyA site"
     regulatory      1712..1717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
                     /note="hexamer: AATAAA"
     polyA_site      1732
                     /gene="Ube2a"
                     /gene_synonym="HHR6A; HR6A; Mhr6a"
ORIGIN      
        1 ccgcgcgttc tccccaggtt ggttcggccc ccgtctactc tggggtggtg cttagccggc
       61 gccagaccga ccctcggctc cggagcggca gtgccgtccc tctcggcgcc tcctcggctt
      121 ctccggcttc ctcggcttcc tcggggaccg cggaccctgg tatatgcccg actgctgagc
      181 ccgctaaagc catgtcgacc ccggcccggc gccgcctcat gagggacttc aagaggttac
      241 aggaagatcc tccggccgga gtgagcgggg ctccgtcgga gaacaacata atggtttgga
      301 atgcggtcat ttttgggccc gaagggaccc cgtttgagga tggaacattt aagcttacaa
      361 ttgaattcac tgaagagtat cctaataagc cacctactgt tagatttgtc tctaagatgt
      421 tccatcctaa cgtctatgca gatggtagta tatgtctgga tatacttcag aaccgttgga
      481 gtccaaccta tgatgtgtct tccattttaa cgtccataca gtctctattg gatgaaccta
      541 atcccaatag tccagcaaac agccaggctg cccagctgta ccaggagaac aagcgggaat
      601 atgaaaagcg tgtttctgca atagtagaac aaagctggcg cgactgttga ctcagggtag
      661 agcagaagaa gaggctggcc ataagaaaaa tgtatattga tgcatttatc accttattcc
      721 taattcatta catttacttt attaaaaaca aaatagctgt tgtgctgttt ctgtcttccc
      781 ttggcaagtt tttcttcccc tttctgtcct cctgtggagc atcagagaca ttatttgaaa
      841 tccaatgtac tgtacctggg ttacttgcaa acattacaat tgcatatgtc cctttgttgt
      901 atctcatctc cagcctcaaa gcagttatca ggttactgta ctttttatac taagctttta
      961 agataaaata ttatgtatag tctggtgacc aggatgttat ttgtaacaaa atgattgctg
     1021 ctgtgtttca tcctggccat tccttccctt gtatttgatt tggcgaagaa tgtatttaga
     1081 atatgaccta aagaaaacag gaatacattc atataaacaa tatttgaaag gggaaggaaa
     1141 gctgaaaaac cctatactca tttgtaagca atatgaacat gaactacaag atggcactta
     1201 ctggtagcaa ggagactgct aaaggaccca gctaacaaac tgaagcaatt ttaataactg
     1261 acacagtcca ctttgtgcga gatattcata gtgtaaacgc attctgtttc ggaattctgg
     1321 ccttcagagt tggtttttat agaggtgtaa gaacaggact agactttttt ttaaagttag
     1381 gaaaaaccga agatcctcta ttctcttaaa ggagcagtgt tgccctaaat gaactttcct
     1441 ttttatgatt cacggaaggg agtgtatctt gcaaactttt cccattcaaa tcctgagtta
     1501 atgctgcatg ctttcagtgt tttgagaacc ttctattgcc catatctgca attttttctt
     1561 tgaatattta cacttttaaa tatacagtat ggggcaaggc ttgtaaatgt tttgtctaat
     1621 atattttcat tgttaatcta ttatgcattt atagcatttc taactttgca caagtaaccc
     1681 atgtaaaaac tgtacatttt caaaagttgt aaataaaaaa ataaccttaa aaaaaaaaaa
     1741 aaaaaa
//
