LOCUS       NM_011666               2632 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ubiquitin-like modifier activating enzyme 3 (Uba3),
            transcript variant 1, mRNA.
ACCESSION   NM_011666
VERSION     NM_011666.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Zhao,M., Zhang,Y., Yang,X., Jin,J., Shen,Z., Feng,X., Zou,T.,
            Deng,L., Cheng,D., Zhang,X., Qin,C., Niu,C., Ye,Z., Zhang,X.,
            He,J., Hou,C., Li,G., Han,G., Cheng,Q., Wang,Q., Wei,L., Dong,J.
            and Zhang,J.
  TITLE     Myeloid neddylation targets IRF7 and promotes host innate immunity
            against RNA viruses
  JOURNAL   PLoS Pathog 17 (9), e1009901 (2021)
   PUBMED   34506605
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Zhang,X., Zhang,Y.L., Qiu,G., Pian,L., Guo,L., Cao,H., Liu,J.,
            Zhao,Y., Li,X., Xu,Z., Huang,X., Huang,J., Dong,J., Shen,B.,
            Wang,H.X., Ying,X., Zhang,W.J., Cao,X. and Zhang,J.
  TITLE     Hepatic neddylation targets and stabilizes electron transfer
            flavoproteins to facilitate fatty acid beta-oxidation
  JOURNAL   Proc Natl Acad Sci U S A 117 (5), 2473-2483 (2020)
   PUBMED   31941714
REFERENCE   3  (bases 1 to 2632)
  AUTHORS   Cheng,Q., Liu,J., Pei,Y., Zhang,Y., Zhou,D., Pan,W. and Zhang,J.
  TITLE     Neddylation contributes to CD4+ T cell-mediated protective immunity
            against blood-stage Plasmodium infection
  JOURNAL   PLoS Pathog 14 (11), e1007440 (2018)
   PUBMED   30462731
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2632)
  AUTHORS   Zhang,X., Ye,Z., Pei,Y., Qiu,G., Wang,Q., Xu,Y., Shen,B. and
            Zhang,J.
  TITLE     Neddylation is required for herpes simplex virus type I
            (HSV-1)-induced early phase interferon-beta production
  JOURNAL   Cell Mol Immunol 13 (5), 578-583 (2016)
   PUBMED   27593482
REFERENCE   5  (bases 1 to 2632)
  AUTHORS   Flomerfelt,F.A., El Kassar,N., Gurunathan,C., Chua,K.S.,
            League,S.C., Schmitz,S., Gershon,T.R., Kapoor,V., Yan,X.Y.,
            Schwartz,R.H. and Gress,R.E.
  TITLE     Tbata modulates thymic stromal cell proliferation and thymus
            function
  JOURNAL   J Exp Med 207 (11), 2521-2532 (2010)
   PUBMED   20937703
REFERENCE   6  (bases 1 to 2632)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 2632)
  AUTHORS   Ohki,Y., Funatsu,N., Konishi,N. and Chiba,T.
  TITLE     The mechanism of poly-NEDD8 chain formation in vitro
  JOURNAL   Biochem Biophys Res Commun 381 (3), 443-447 (2009)
   PUBMED   19245792
REFERENCE   8  (bases 1 to 2632)
  AUTHORS   Artus,J., Babinet,C. and Cohen-Tannoudji,M.
  TITLE     The cell cycle of early mammalian embryos: lessons from genetic
            mouse models
  JOURNAL   Cell Cycle 5 (5), 499-502 (2006)
   PUBMED   16481745
  REMARK    Review article
REFERENCE   9  (bases 1 to 2632)
  AUTHORS   Tateishi,K., Omata,M., Tanaka,K. and Chiba,T.
  TITLE     The NEDD8 system is essential for cell cycle progression and
            morphogenetic pathway in mice
  JOURNAL   J Cell Biol 155 (4), 571-579 (2001)
   PUBMED   11696557
REFERENCE   10 (bases 1 to 2632)
  AUTHORS   Gong,L. and Yeh,E.T.
  TITLE     Identification of the activating and conjugating enzymes of the
            NEDD8 conjugation pathway
  JOURNAL   J Biol Chem 274 (17), 12036-12042 (1999)
   PUBMED   10207026
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK159381.1, AK166655.1 and
            BY639644.1.
            
            On Sep 23, 2014 this sequence version replaced NM_011666.2.
            
            Summary: The protein encoded by this gene is the catalytic subunit
            of the enzyme that activates NEDD8, a ubiquitin-like molecule that
            binds to its target proteins through an enzymatic reaction
            analagous to ubiquitylation. Embryonic mice deficient for this
            protein die prior to implantation and display apoptosis of the
            inner cell mass. Trophoblastic cells cannot enter S phase,
            demonstrating that this gene is required for cell cycle progression
            during embryogenesis. Two pseudogenes have been found for this
            gene. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Sep 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048148.1, SRR1660813.230357.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1512              AK159381.1         2-1513
            1513-2594           AK166655.1         1384-2465
            2595-2632           BY639644.1         369-406
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 44.9 cM"
     gene            1..2632
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /note="ubiquitin-like modifier activating enzyme 3"
                     /db_xref="GeneID:22200"
                     /db_xref="MGI:MGI:1341217"
     exon            1..115
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1484
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /EC_number="6.2.1.64"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NEDD8-activating enzyme E1 catalytic subunit;
                     NEDD8-activating enzyme E1C; ubiquitin-activating enzyme
                     3; ubiquitin-activating enzyme E1C"
                     /codon_start=1
                     /product="NEDD8-activating enzyme E1 catalytic subunit
                     isoform 1"
                     /protein_id="NP_035796.2"
                     /db_xref="CCDS:CCDS51860.1"
                     /db_xref="GeneID:22200"
                     /db_xref="MGI:MGI:1341217"
                     /translation="MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKF
                     LERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMD
                     TIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFH
                     IIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMT
                     ACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDDPE
                     HIQWIFQKSIERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS
                     AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNS
                     ASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT
                     TPQTVLFKLHFT"
     exon            116..157
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            158..278
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            279..359
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            360..442
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            443..523
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            524..567
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            568..632
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            633..788
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            789..891
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1005
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1059
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1096
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1178
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1279
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1343
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1398
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..2632
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2229..2234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /note="hexamer: AATAAA"
     polyA_site      2261
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /note="major polyA site"
     regulatory      2281..2286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /note="hexamer: AGTAAA"
     polyA_site      2311
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
     regulatory      2429..2434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
                     /note="hexamer: AATGAA"
     polyA_site      2448
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
     polyA_site      2581
                     /gene="Uba3"
                     /gene_synonym="A830034N06Rik; Ube1c"
ORIGIN      
        1 ggctcggctc tccctccttc cattcacctg cagtcccgcc cctcccgggc ggacccgctc
       61 ggcgcgaggg cctctgggaa gaggcgggga acaatatggc ggatggcgag gagccggaga
      121 agaaaagaag gagaatagag gagctgctgg ctgagaaaat ggctgttgat ggtgggtgtg
      181 gggacactgg agactgggaa ggtcgctgga accatgtaaa gaagttcctc gagcggtctg
      241 gacccttcac acaccccgat ttcgaaccaa gcactgaatc actccagttc ttgttagata
      301 catgtaaagt tctagtcatt ggagctggtg gcttaggatg tgagcttctg aaaaatctgg
      361 cattatctgg ttttagacag attcatgtta tagacatgga cactatagat gtttccaatt
      421 taaatagaca gtttttattt aggcctaaag atgtcggaag acccaaggct gaagttgctg
      481 cagaattcct aaatgacaga gttcctaact gcaacgtggt accacatttc aacaagatac
      541 aagattttaa cgacactttc taccgacaat ttcatatcat tgtatgtggc ctggactcta
      601 tcatagcgag aagatggatc aatggaatgc tgatatctct tctaaattat gaagatggtg
      661 tgttggatcc aagctccatt gtacctttga tagatggggg gacagaaggc tttaaaggga
      721 atgcccgagt gattttgcct ggaatgaccg cttgtattga gtgcactctg gaactttacc
      781 caccacaggt caatttcccc atgtgtacca ttgcatctat gcccaggctc ccagaacact
      841 gtatcgagta tgtgaggatg ttgcaatggc ctaaagagca gccttttgga gatggggttc
      901 cattagatgg agatgaccct gaacatattc agtggatttt ccaaaagtcc atagagagag
      961 catcacaata taatattaga ggcgttacct acagactcac tcaaggggtg gtaaaacgaa
     1021 tcattcctgc agtagcttct acaaatgcag tcattgcagc tgtgtgtgcc actgaggttt
     1081 tcaagatagc tacaagtgcg tacattcccc ttaataacta cctggtattc aatgatgtag
     1141 atgggctgta cacttacacg tttgaagcag agagaaagga aaactgtcca gcatgtagcc
     1201 aacttcctca aaacattcag ttttccccat cagctaaact acaggaggtc ttagactacc
     1261 taaccaacag tgcttctctg caaatgaagt ctccggctat cacagccaca ttagagggga
     1321 agaacaggac actttactta cagtcagtaa cgtctattga agaacgaacc aggcccaatc
     1381 tttccaaaac attaaaagaa ctgggactag ttgatggaca agaactggct gttgctgatg
     1441 tcactacacc acagactgta ctattcaaac ttcattttac ttaaggaaaa taaatctgca
     1501 cataatagaa aattcataga aataatatac tttataaatg atatgaaatt gaagagcctg
     1561 gaagatgagg cagaggggaa catccaagaa aggaaattta attggtgtca tttttagcat
     1621 tagtgtggct agaatttgac ttttatatat atacatatat ataaaaaagg actgactctt
     1681 ttttaacttt ataagtttct cttgaagact gaactttggg gttgggctag caagcatttt
     1741 cattttatta ctatggaaag ctatgccttc aggagagatt atgaacaagt gtgttgcttc
     1801 tttaaagcag gacaaacact gtcttgtgtg tgagtttgtt gtggtcaaag agcatattcc
     1861 tcagcgtgta tctgaaatcc acatgtgtag aaatgtctcc tgggatggaa atgaggagct
     1921 atgtctgaag aatagtaaat attcacagcc tgacatctag agtatatcaa acataggcag
     1981 tgtcttcatt gctactcata taattgtgac tatccatgtg tgtattaatt attgcagaat
     2041 ttaacttgtc catgataatt tgtaaacagt attatagatt catacctgtg catgaaaata
     2101 caaaatattt tcatgtattt gtttgcaatg ccacagagac cagtatgcac aaatttaaac
     2161 caagacatgg ctgttcaaag aaaattaatg tttaaacagt tatcattgat gcttttgcac
     2221 tatttattaa taaaattgta cattgtcttt ttattggaaa acttaaagta tatcaataat
     2281 agtaaataac tttatagtga tgtagaaata atttttaaat gtttaccagt tattatttcc
     2341 agacttttat tgcgcctgag ttaaattttt aaaattttat tatagtgatt tccatagtac
     2401 agactaggtt cactcattct aactagtaaa tgaaatggaa tgacttaatt tttatagtca
     2461 agtctctctc ttagcatgcc tttttttttc ttcaaaaatg ctaaaatcaa agtggagcac
     2521 gaatactaaa aaaatgtagt tgtaatattt taatgtgggg acaaatagta cctgatttta
     2581 attctttgtt tttcatttaa ttgcaatttt ttattcagac tgttagtggg tg
//
