LOCUS       NM_001418476            2553 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2),
            transcript variant 5, mRNA.
ACCESSION   NM_001418476
VERSION     NM_001418476.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2553)
  AUTHORS   Zhao,Y., Zheng,D. and Cvekl,A.
  TITLE     A comprehensive spatial-temporal transcriptomic analysis of
            differentiating nascent mouse lens epithelial and fiber cells
  JOURNAL   Exp Eye Res 175, 56-72 (2018)
   PUBMED   29883638
REFERENCE   4  (bases 1 to 2553)
  AUTHORS   Kanemaru,A. and Saitoh,H.
  TITLE     High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating
            enzyme, mAU, in a baculovirus-insect cell system
  JOURNAL   Biosci Biotechnol Biochem 77 (7), 1575-1578 (2013)
   PUBMED   23832333
REFERENCE   5  (bases 1 to 2553)
  AUTHORS   Costa,M.W., Lee,S., Furtado,M.B., Xin,L., Sparrow,D.B.,
            Martinez,C.G., Dunwoodie,S.L., Kurtenbach,E., Mohun,T.,
            Rosenthal,N. and Harvey,R.P.
  TITLE     Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5
  JOURNAL   PLoS One 6 (9), e24812 (2011)
   PUBMED   21931855
REFERENCE   6  (bases 1 to 2553)
  AUTHORS   Takahashi,H., Hatakeyama,S., Saitoh,H. and Nakayama,K.I.
  TITLE     Noncovalent SUMO-1 binding activity of thymine DNA glycosylase
            (TDG) is required for its SUMO-1 modification and colocalization
            with the promyelocytic leukemia protein
  JOURNAL   J Biol Chem 280 (7), 5611-5621 (2005)
   PUBMED   15569683
REFERENCE   7  (bases 1 to 2553)
  AUTHORS   Uchimura,Y., Nakao,M. and Saitoh,H.
  TITLE     Generation of SUMO-1 modified proteins in E. coli: towards
            understanding the biochemistry/structural biology of the SUMO-1
            pathway
  JOURNAL   FEBS Lett 564 (1-2), 85-90 (2004)
   PUBMED   15094046
REFERENCE   8  (bases 1 to 2553)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2553)
  AUTHORS   Azuma,Y., Tan,S.H., Cavenagh,M.M., Ainsztein,A.M., Saitoh,H. and
            Dasso,M.
  TITLE     Expression and regulation of the mammalian SUMO-1 E1 enzyme
  JOURNAL   FASEB J 15 (10), 1825-1827 (2001)
   PUBMED   11481243
REFERENCE   10 (bases 1 to 2553)
  AUTHORS   Schwienhorst,I., Johnson,E.S. and Dohmen,R.J.
  TITLE     SUMO conjugation and deconjugation
  JOURNAL   Mol Gen Genet 263 (5), 771-786 (2000)
   PUBMED   10905345
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX537302.12.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422597.1094642.1,
                                           SRR17784644.440658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               BX537302.12        31714-31898         c
            186-269             BX537302.12        28743-28826         c
            270-340             BX537302.12        27726-27796         c
            341-405             BX537302.12        26495-26559         c
            406-506             BX537302.12        24878-24978         c
            507-628             BX537302.12        22159-22280         c
            629-696             BX537302.12        21320-21387         c
            697-818             BX537302.12        20584-20705         c
            819-918             BX537302.12        19226-19325         c
            919-1079            BX537302.12        17825-17985         c
            1080-1173           BX537302.12        14336-14429         c
            1174-1286           BX537302.12        14129-14241         c
            1287-1446           BX537302.12        10857-11016         c
            1447-1539           BX537302.12        9546-9638           c
            1540-1645           BX537302.12        7831-7936           c
            1646-1782           BX537302.12        6079-6215           c
            1783-2553           BX537302.12        4037-4807           c
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 19.41 cM"
     gene            1..2553
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /note="ubiquitin-like modifier activating enzyme 2"
                     /db_xref="GeneID:50995"
                     /db_xref="MGI:MGI:1858313"
     exon            1..185
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1964
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /EC_number="6.3.2.-"
                     /note="isoform 5 is encoded by transcript variant 5;
                     ubiquitin-like 1 (sentrin) activating enzyme E1B;
                     ubiquitin-like 1 (sentrin) activating enzyme subunit 2;
                     SUMO-activating enzyme subunit 2; ubiquitin-like
                     1-activating enzyme E1B; anthracycline-associated
                     resistance ARX; Sumo-1 activating enzyme subunit 2; SUMO1
                     activating enzyme subunit 2"
                     /codon_start=1
                     /product="SUMO-activating enzyme subunit 2 isoform 5"
                     /protein_id="NP_001405405.1"
                     /db_xref="GeneID:50995"
                     /db_xref="MGI:MGI:1858313"
                     /translation="MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHI
                     DLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFHPQANIEAHHDSIMNPDYN
                     VEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYE
                     CHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWE
                     PTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKR
                     KPPVPLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEK
                     GDGAELIWDKDDPPAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVI
                     AGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNTNCYVCASKPEVTV
                     RLNVHKVTVLTLQDKAMKEKFAMVAPDVQIEDGKGTILISSEEGETEANNPKKLSDFG
                     IRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDAAKSIAN
                     GSDDGAQPSTSTAQEQDDVLIVDSDEEGPSNSTDCSGDDKARKRKLEENEAASTKKCR
                     LEQMEDPDDVIALD"
     exon            186..269
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            270..340
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            341..405
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            406..506
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            507..628
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            629..696
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            697..818
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            819..918
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1079
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1173
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1286
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1446
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1539
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1645
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1782
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..2553
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2530..2535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /note="hexamer: AATAAA"
     polyA_site      2553
                     /gene="Uba2"
                     /gene_synonym="Arx; Sae2; UBA1; Ubl1a2; Uble1b"
                     /note="major polyA site"
ORIGIN      
        1 ctggttctcc cgcctccgac tgtgaggcgg ctcgtagacg tcccgccatg gcactgtcgc
       61 gggggttgcc ccgggagctg gccgaggcgg tgtccggcgg ccgggtgctg gtggttggcg
      121 cgggtgggat cggctgtgag ctgctcaaga acctggtgct cactggcttc tcccacatcg
      181 acctgattga tctggacact atcgatgtca gcaacctcaa caggcagttc ttgtttcaaa
      241 agaaacatgt gggaagatca aaggctcagg ttgccaaaga aagtgtgctc cagttccacc
      301 cccaagccaa cattgaggct caccatgaca gcatcatgaa cccagactat aatgtggagt
      361 tttttcgaca gtttatattg gtcatgaatg cattagataa cagagctgca cgaaaccacg
      421 tgaataggat gtgtctggcc gctgatgtgc ctctcattga gagcgggact gctgggtatc
      481 tcggacaggt gaccactatc aagaagggtg tgactgagtg ttacgaatgt caccctaagc
      541 ctacccagag gacgttccct ggctgtacga ttcggaacac accttcagaa cctatccatt
      601 gcatcgtatg ggccaagtat ttgttcaacc agctgtttgg agaggaagat gctgatcaag
      661 aagtgtctcc tgacagagct gaccctgagg ctgcttggga accaacagag gctgaagcta
      721 gagctcgagc atctaatgaa gacggagaca ttaagcgtat ttccactaag gaatgggcta
      781 agtcaactgg atatgatcca gttaaacttt ttaccaagct ttttaaagat gatattagat
      841 atctgctgac aatggacaaa ctctggcgca agaggaaacc tccagttcca ctggactggg
      901 ctgaagtgca gagtcaagga gaagccaatg ccgatcaaca aaatgagccc cagttgggtt
      961 tgaaagacca gcaggttctg gatgtaaaga gctatgcgag tctgttttca aagagcatcg
     1021 agactctgcg agtccattta gcggagaagg gggatggagc tgagctcatc tgggataagg
     1081 acgacccacc tgcaatggat tttgttacat ctgctgcaaa cctcaggatg cacattttca
     1141 gcatgaatat gaagagcagg tttgatataa aatcaatggc agggaatatc attcctgcta
     1201 tagctactac taatgcagtg atcgccggct tgatagtgtt ggaaggatta aagattttat
     1261 ctggaaaaat agaccagtgt agaactattt ttttgaataa gcagccaaac ccaagaaaga
     1321 agctcttggt gccctgtgca ctggatcctc ccaacaccaa ctgttacgtt tgtgccagca
     1381 agccagaggt gactgtgcgg ctgaatgtcc acaaagtgac tgtcctcacc ttacaggata
     1441 aggcaatgaa agaaaagttt gctatggtgg caccagatgt ccaaattgaa gatgggaaag
     1501 gcacaatcct gatatcttca gaagagggag agacagaagc caataacccc aagaagttgt
     1561 ctgattttgg aattagaaac ggcagccggc ttcaagcaga cgacttcctc caggactata
     1621 ctttgctgat caacattctt catagtgaag atcttggaaa ggatgttgaa tttgaagtag
     1681 ttggtgacag ccctgaaaaa gtggggccaa aacaagctga agatgctgcc aaaagcattg
     1741 ccaacggcag cgatgacggt gcccagcctt ccacctccac agcacaagag caagatgatg
     1801 tgctcatagt tgattcagac gaagaaggcc cttcaaatag caccgactgc agcggggacg
     1861 acaaggcccg caagcggaag ctggaagaga atgaggctgc cagtaccaag aagtgccgct
     1921 tggagcagat ggaagatcca gatgacgtca tagcgttaga ctgagcaggc tcctgccctt
     1981 ggtctggtcg gaaccaaaag ttagatcctt tgtgtggctt tgatggttcc gttcagcgtt
     2041 catttgctga gactgttcga gactgttgct gtgttgaagt aggaatagaa tttgcaaccc
     2101 tttgagcaaa gctgggtgat gagctgaaag aacgcgggcg tgcatgttgc cttccctgta
     2161 aatagcctta gctatcattt cctagtggaa aacactgtgg ggtaggaacg ctgcaacctg
     2221 ggtataaata ttatgccttt atttttggct agagaaaagt tatttttagc ctagatctaa
     2281 ccattttcat actcttacct gaaagattca cagaagtgtc aagtgctatg cattgaaact
     2341 tgttttttaa acattacctg gcactgtgta tattaatgta aaacaatgtt aatttaccca
     2401 gattttcagt ttgtactgcc tggtatgtct gtgtaagaag ccaatttttg tgtatttgtt
     2461 acagtttcaa gttatttata tttgatgttt tgtaaactca aacaacaaat atactgtact
     2521 tatggaccaa ataaatgatg tctgcatact tgt
//
