LOCUS       NM_009457               4068 bp    mRNA    linear   ROD 14-DEC-2022
DEFINITION  Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1),
            transcript variant 1, mRNA.
ACCESSION   NM_009457
VERSION     NM_009457.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Liu H, He L, Cheng Q, Luo W, Zhao T and Yang D.
  TITLE     Validation of superior reference genes in mouse submandibular
            glands under developmental and functional regeneration states
  JOURNAL   Int J Mol Med 50 (5) (2022)
   PUBMED   36069228
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Chomiak AA, Guo Y, Kopsidas CA, McDaniel DP, Lowe CC, Pan H, Zhou
            X, Zhou Q, Doughty ML and Feng Y.
  TITLE     Nde1 is required for heterochromatin compaction and stability in
            neocortical neurons
  JOURNAL   iScience 25 (6), 104354 (2022)
   PUBMED   35601919
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4068)
  AUTHORS   Kimura-Yoshida C, Mochida K, Kanno SI and Matsuo I.
  TITLE     USP39 is essential for mammalian epithelial morphogenesis through
            upregulation of planar cell polarity components
  JOURNAL   Commun Biol 5 (1), 378 (2022)
   PUBMED   35440748
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4068)
  AUTHORS   Liao J, Yang X, Lin Q, Liu S, Xie Y, Xia Y and Li HH.
  TITLE     Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses
            Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice
  JOURNAL   J Immunol Res 2020, 7812709 (2020)
   PUBMED   32258175
  REMARK    GeneRIF: Inhibition of the Ubiquitin-Activating Enzyme UBA1
            Suppresses Diet-Induced Atherosclerosis in Apolipoprotein
            E-Knockout Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4068)
  AUTHORS   Alvarez-Dominguez JR, Zhang X and Hu W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   6  (bases 1 to 4068)
  AUTHORS   Nagai Y, Kaneda S, Nomura K, Yasuda H, Seno T and Yamao F.
  TITLE     Ubiquitin-activating enzyme, E1, is phosphorylated in mammalian
            cells by the protein kinase Cdc2
  JOURNAL   J Cell Sci 108 (Pt 6), 2145-2152 (1995)
   PUBMED   7673335
REFERENCE   7  (bases 1 to 4068)
  AUTHORS   Imai N, Kaneda S, Nagai Y, Seno T, Ayusawa D, Hanaoka F and Yamao
            F.
  TITLE     Cloning and sequence of a functionally active cDNA encoding the
            mouse ubiquitin-activating enzyme E1
  JOURNAL   Gene 118 (2), 279-282 (1992)
   PUBMED   1511901
REFERENCE   8  (bases 1 to 4068)
  AUTHORS   Disteche CM, Zacksenhaus E, Adler DA, Bressler SL, Keitz BT and
            Chapman VM.
  TITLE     Mapping and expression of the ubiquitin-activating enzyme E1 (Ube1)
            gene in the mouse
  JOURNAL   Mamm Genome 3 (3), 156-161 (1992)
   PUBMED   1617221
REFERENCE   9  (bases 1 to 4068)
  AUTHORS   Kay GF, Ashworth A, Penny GD, Dunlop M, Swift S, Brockdorff N and
            Rastan S.
  TITLE     A candidate spermatogenesis gene on the mouse Y chromosome is
            homologous to ubiquitin-activating enzyme E1
  JOURNAL   Nature 354 (6353), 486-489 (1991)
   PUBMED   1749428
REFERENCE   10 (bases 1 to 4068)
  AUTHORS   Mitchell MJ, Woods DR, Tucker PK, Opp JS and Bishop CE.
  TITLE     Homology of a candidate spermatogenic gene from the mouse Y
            chromosome to the ubiquitin-activating enzyme E1
  JOURNAL   Nature 354 (6353), 483-486 (1991)
   PUBMED   1684224
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY004827.1, BC145984.1 and
            AK143416.1.
            
            [WARNING] On Dec 4, 2023 this sequence was replaced by NM_009457.5.
            
            On Jan 29, 2013 this sequence version replaced NM_009457.3.
            
            Summary: This gene encodes a member of the ubiquitin-activating E1
            family. The encoded protein initiates the ubiquitin activation and
            transfer cascade, catalyzing the first step in ubiquitin
            conjugation to mark cellular proteins for proteasome degradation.
            Ubiquitin activating enzymes use ATP to form a thioester between a
            conserved catalytic cysteine of the enzyme and the C-terminal
            carboxylate of ubiquitin. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (1) uses an alternate 5' exon
            structure and an upstream start codon, compared to variant 3. The
            encoded isoform (1) has a longer N-terminus, compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088057.1, SRR1660815.163696.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               BY004827.1         3-138
            137-3443            BC145984.1         1-3307
            3444-4068           AK143416.1         3350-3974
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 16.15 cM"
     gene            1..4068
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /note="ubiquitin-like modifier activating enzyme 1"
                     /db_xref="GeneID:22201"
                     /db_xref="MGI:MGI:98890"
     exon            1..225
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..3402
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /EC_number="6.2.1.45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-activating enzyme E1, Chr X"
                     /codon_start=1
                     /product="ubiquitin-like modifier-activating enzyme 1
                     isoform 1"
                     /protein_id="NP_033483.2"
                     /db_xref="GeneID:22201"
                     /db_xref="MGI:MGI:98890"
                     /translation="MPLYSGVYGIPQTRPSKAPALPSSAQTLVLFARSRWFALPLPCG
                     FGLSPEGRRGFRPALGMSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMA
                     KNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVT
                     LHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSS
                     FQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQP
                     LSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYT
                     FSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIG
                     FQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVA
                     AGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQ
                     NRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMD
                     TIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDF
                     FQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQ
                     DPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLA
                     GTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSG
                     APFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQV
                     PEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDD
                     SNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKV
                     VQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEM
                     TLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLG
                     RHVRALVLELCCNDESGEDVEVPYVRYTIR"
     exon            226..342
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            343..401
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            402..570
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            571..705
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            706..812
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            813..903
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1036
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1134
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1281
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1458
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1563
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1644
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1800
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1966
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2163
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2228
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2424
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2499
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2689
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2778
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2871
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3063
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3165
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..3266
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..4068
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3574..3579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
     polyA_site      3593
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
     regulatory      4046..4051
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
     polyA_site      4065
                     /gene="Uba1"
                     /gene_synonym="A1S9; Sbx; Ube-1; Ube1x"
ORIGIN      
        1 tttttgtccc tgaaacccgg gccagaggcg cagaagtaga gagaaatgcc cctctattca
       61 ggcgtctatg gtatacccca gacccggccc tctaaggccc ccgcgcttcc gagctccgcg
      121 caaactctgg ttctttttgc acgatcgagg tggtttgctc ttccgttgcc ctgtggcttc
      181 ggcttgtctc cagaaggaag gcgaggcttc cgcccagcac tggggatgtc cagctcgccg
      241 ctgtccaaga aacgtcgcgt gtccgggcct gatccaaagc cgggttctaa ctgctcccct
      301 gcacagtctg cgctgtccga agtgtcctca gtgccaacca acggaatggc gaagaacggc
      361 agtgaagcag acatagacga gagcctttac tcccggcagc tgtacgtttt gggccatgag
      421 gcaatgaaaa tgctccagac atccagcgtc cttgtctcag gcttgcgggg cttgggtgta
      481 gaaattgcta agaacatcat ccttggtggg gtcaaggctg tcaccctaca tgaccaagga
      541 actacccagt gggctgatct ctcctcccag ttttaccttc gggaggagga cattggtaaa
      601 aatcgagcgg aggtatccca gccccgactt gctgaactca acagctatgt acctgtcact
      661 gcctacactg ggcctcttgt cgaggacttc cttagtagct tccaggtggt ggtcctcacc
      721 aacagccccc tggaagccca gctgcgagtg ggggagttct gtcatagccg tggtatcaag
      781 ctagtggtgg cagatacaag aggcctgttt gggcaacttt tctgtgattt tggagaggaa
      841 atggtcctca cagattccaa tggggagcag ccactcagtg ctatggtttc aatggtcacc
      901 aaggacaacc ccggtgtggt tacctgcctt gatgaggccc gacatggctt tgagactggt
      961 gacttcgtct cattctcaga agtacagggc atgatccaac tcaatggatg tcagcccatg
     1021 gagatcaaag tgctgggtcc ttataccttt agtatctgtg acacttccaa cttctctgac
     1081 tacatccgtg gaggcatcgt cagtcaggtc aaagtaccga aaaagattag ttttaaatcc
     1141 ttgccagcat cactggtaga gcctgacttt gtgatgactg actttgccaa gtattctcgc
     1201 cctgcccaac tgcacattgg cttccaagct ctgcaccaat tctgtgctct gcacaaccaa
     1261 ccacctcgac cacgaaatga ggaagatgca acagagctgg tgggcctggc tcaggctgta
     1321 aacgctcggt ccccaccttc agtaaaacag aacagcttgg atgaagacct tattcggaag
     1381 ctagcttatg ttgctgctgg ggacctggca cccataaatg ctttcattgg gggccttgct
     1441 gcccaggaag tcatgaaggc ctgctctgga aagtttatgc ccatcatgca gtggttgtac
     1501 tttgatgctc ttgaatgtct cccagaggac aaagaggctc tgacagagga gaagtgcctc
     1561 ccacgtcaga accgttacga tgggcaggta gctgtatttg ggtcagactt tcaggagaag
     1621 ctgagcaagc aaaagtactt cctggtgggt gcaggggcca ttggctgtga attgctcaag
     1681 aactttgcca tgattgggct gggttgtgga gagggtggag aggtcgtggt cacagacatg
     1741 gacaccattg agaaatcaaa tctgaaccga cagtttctct tccggccctg ggatgtcacg
     1801 aagttaaagt ctgacacggc cgctgcagct gtgcgccaga tgaatcctta catccaggtg
     1861 acaagccacc agaaccgtgt aggtcctgac actgagcgca tctatgatga tgatttcttc
     1921 caaaatttgg atggtgtggc caatgctctg gacaacatag atgcccgcat gtacatggat
     1981 cgccgatgtg tgtactatcg gaagccactg ctagagtctg gcacactggg cacaaagggc
     2041 aacgtgcagg tggtaatccc cttcctgaca gaatcctaca gctctagcca ggatccacca
     2101 gagaaatcca tccccatttg taccctgaaa aactttccca atgccatcga acacactctt
     2161 cagtgggccc gggatgaatt tgaaggcctt ttcaagcagc cagcagaaaa tgttaatcag
     2221 tacctcacag actccaaatt tgtggagcgg acattgcggc tggctggtac ccagccattg
     2281 gaggtgctgg aggctgtgca gcgcagcctg gtgttgcagc gaccacagac ttggggagac
     2341 tgtgtgacct gggcctgcca ccactggcac acccagtact gtaacaacat ccggcaactg
     2401 ctgcacaact ttcctcctga ccagctcacc agctcagggg cccctttctg gtctggaccc
     2461 aaacgctgtc cacacccact tacttttgat gttaacaata cattgcatct ggattatgtg
     2521 atggctgctg ccaacctttt tgcccagacc tatgggttga ctggatccca agaccgagct
     2581 gctgtggcct cactcctgca gtcagtacaa gtcccagagt tcacccccaa gtctggtgtc
     2641 aagattcatg tttctgacca ggagttgcag agtgccaatg cctctgttga tgacagccgt
     2701 cttgaggagc tcaaagccac attgcccagc ccagacaagt tacccggatt taagatgtac
     2761 cccattgatt ttgagaagga tgatgacagc aatttccaca tggatttcat tgtggctgca
     2821 tccaatcttc gggccgaaaa ctatgatatt tcccctgcag accgacacaa gagcaagctg
     2881 attgcaggga agatcatccc agccattgcc acaaccacag ctgctgtggt tggccttgtg
     2941 tgtctggagc tctacaaggt agttcaaggg caccaacagc tcgattccta caaaaatggt
     3001 ttcctgaact tggccctgcc cttctttggg ttttctgaac ctcttgctgc acctcgtcac
     3061 cagtactata atcaagagtg gacattgtgg gatcgctttg aagtacaagg gctgcagcct
     3121 aatggtgagg agatgaccct caagcagttc cttgattact ttaagacaga gcacaaattg
     3181 gagatcacca tgctgtccca gggcgtgtcc atgctctatt ctttcttcat gccagctgct
     3241 aagctcaagg aacgattgga tcagccgatg acagagattg tgagccgagt gtcaaagaga
     3301 aagctgggcc gccatgtgcg ggcactggtg cttgagctgt gctgcaacga tgaaagcggc
     3361 gaggacgtcg aggtccctta tgtccgatat accattcgct gatctccgtg cattccccta
     3421 agccccagtc ctccctcctc agatcctttc caatcctggt ttccatttgg cctcaggcag
     3481 tggtccatgt agccaagcct ggccttccct attacccagc aacctcaaga ccctctcacc
     3541 gctgctttgt accttgtttg gaacctaaat cccaataaaa ggataattac ccagatgtag
     3601 ccatcctggc tgtctccaag gaaggaagaa gtatgtctag agttctctgg ttcaaataag
     3661 taccccaatg ggctactcca gctataagct ttctgggcag agaaaactgg gaattgatat
     3721 cttttatata ctctgcataa cagacatgag gtaccagtga agttatgcag accagtacct
     3781 gggtcaatat gatttaagtg acttgaactg ggttcaaact ctgtcttctc catgatcact
     3841 actagctgcc tccatccact atgaagaata cacttaacag tgatcaggtg ggggctggag
     3901 agacggctca gctcttaaga gcactgactg ctcttccagg ggtcctgagt tcaattccca
     3961 gcaaccacat ggtggctcac aaccatctgt aatggtgggt ctgaagggag tgacagtata
     4021 cacacataca atcagtcaat caatcaataa aaacagtgat caggctgc
//
