LOCUS       NM_021288               3798 bp    mRNA    linear   ROD 11-SEP-2024
DEFINITION  Mus musculus thymidylate synthase (Tyms), transcript variant 1,
            mRNA.
ACCESSION   NM_021288 XM_131960
VERSION     NM_021288.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Guijarro,M.V., Nawab,A., Dib,P., Burkett,S., Luo,X., Feely,M.,
            Nasri,E., Seifert,R.P., Kaye,F.J. and Zajac-Kaye,M.
  TITLE     TYMS promotes genomic instability and tumor progression in
            Ink4a/Arf null background
  JOURNAL   Oncogene 42 (23), 1926-1939 (2023)
   PUBMED   37106126
  REMARK    GeneRIF: TYMS promotes genomic instability and tumor progression in
            Ink4a/Arf null background.
REFERENCE   3  (bases 1 to 3798)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3798)
  AUTHORS   Prokopowicz,M., Jarmula,A., Casamayou-Boucau,Y., Gordon,F.,
            Ryder,A., Sobich,J., Maj,P., Ciesla,J., Zielinski,Z., Fita,P. and
            Rode,W.
  TITLE     Advanced Spectroscopy and APBS Modeling for Determination of the
            Role of His190 and Trp103 in Mouse Thymidylate Synthase Interaction
            with Selected dUMP Analogues
  JOURNAL   Int J Mol Sci 22 (5), 2661 (2021)
   PUBMED   33800923
  REMARK    GeneRIF: Advanced Spectroscopy and APBS Modeling for Determination
            of the Role of His190 and Trp103 in Mouse Thymidylate Synthase
            Interaction with Selected dUMP Analogues.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3798)
  AUTHORS   Harendza,C.J. and Johnson,L.F.
  TITLE     Polyadenylylation signal of the mouse thymidylate synthase gene was
            created by insertion of an L1 repetitive element downstream of the
            open reading frame
  JOURNAL   Proc Natl Acad Sci U S A 87 (7), 2531-2535 (1990)
   PUBMED   2157203
REFERENCE   6  (bases 1 to 3798)
  AUTHORS   Li,D.W. and Johnson,L.F.
  TITLE     A mouse thymidylate synthase pseudogene derived from an aberrantly
            processed RNA molecule
  JOURNAL   Gene 82 (2), 363-370 (1989)
   PUBMED   2573561
REFERENCE   7  (bases 1 to 3798)
  AUTHORS   Deng,T.L., Li,Y. and Johnson,L.F.
  TITLE     Thymidylate synthase gene expression is stimulated by some (but not
            all) introns
  JOURNAL   Nucleic Acids Res 17 (2), 645-658 (1989)
   PUBMED   2915925
REFERENCE   8  (bases 1 to 3798)
  AUTHORS   DeWille,J.W., Jenh,C.H., Deng,T., Harendza,C.J. and Johnson,L.F.
  TITLE     Construction and expression of mouse thymidylate synthase minigenes
  JOURNAL   J Biol Chem 263 (1), 84-91 (1988)
   PUBMED   2826452
REFERENCE   9  (bases 1 to 3798)
  AUTHORS   Deng,T.L., Li,D.W., Jenh,C.H. and Johnson,L.F.
  TITLE     Structure of the gene for mouse thymidylate synthase. Locations of
            introns and multiple transcriptional start sites
  JOURNAL   J Biol Chem 261 (34), 16000-16005 (1986)
   PUBMED   3782103
REFERENCE   10 (bases 1 to 3798)
  AUTHORS   Jenh,C.H., Deng,T.L., Li,D.W., DeWille,J. and Johnson,L.F.
  TITLE     Mouse thymidylate synthase messenger RNA lacks a 3' untranslated
            region
  JOURNAL   Proc Natl Acad Sci U S A 83 (22), 8482-8486 (1986)
   PUBMED   3022294
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC112933.16 and AK035663.1.
            
            On Mar 30, 2010 this sequence version replaced NM_021288.3.
            
            Summary: This gene encodes an enzyme that catalyzes the methylation
            of deoxyuridylate to deoxythymidylate using
            5,10-methylenetetrahydrofolate as a cofactor. This function
            maintains the thymidine-5-prime monophosphate concentration
            critical for DNA replication and repair. The encoded enzyme is a
            target for cancer chemotherapeutic agents. The majority of
            transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407).
            The stop codon in these transcripts is UAA, compared to the UAG
            found in the genome and longer transcripts, as the polyA site is
            located within the stop codon (PMID: 3444407, 2157203). A related
            pseudogene has been identified on chromosome 10. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035663.1, SRR9219380.3980.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC112933.16        129696-129739       c
            45-3171             AK035663.1         2-3128
            3172-3798           AC112933.16        114314-114940       c
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 15.81 cM"
     gene            1..3798
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="thymidylate synthase"
                     /db_xref="GeneID:22171"
                     /db_xref="MGI:MGI:98878"
     exon            1..265
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            266..339
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            340..514
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            515..616
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            617..792
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            793..864
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     exon            865..3798
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /inference="alignment:Splign:2.1.0"
     regulatory      979..984
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="hexamer: ATTAAA"
     polyA_site      1001
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="major polyA site"
     polyA_site      1025
                     /gene="Tyms"
                     /gene_synonym="Ts"
     polyA_site      1048
                     /gene="Tyms"
                     /gene_synonym="Ts"
     regulatory      2414..2419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="hexamer: CATAAA"
     polyA_site      2424
                     /gene="Tyms"
                     /gene_synonym="Ts"
     regulatory      2569..2574
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="hexamer: AGTAAA"
     polyA_site      2588
                     /gene="Tyms"
                     /gene_synonym="Ts"
     polyA_site      3206
                     /gene="Tyms"
                     /gene_synonym="Ts"
     polyA_site      3219
                     /gene="Tyms"
                     /gene_synonym="Ts"
     regulatory      3250..3255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tyms"
                     /gene_synonym="Ts"
                     /note="hexamer: AGTAAA"
     polyA_site      3291
                     /gene="Tyms"
                     /gene_synonym="Ts"
ORIGIN      
        1 gggctggtgt tggaggaaaa gagcgccagg aaggtcctgg ttttgtcgct gactacactg
       61 ctgccagact gctccgttat gctggtggtt ggctccgagc tgcagtccga tgctcagcag
      121 ctgagcgcgg aagccccacg gcatggagaa ctccagtacc tgaggcaggt ggagcacatt
      181 ttgcgctgcg gcttcaagaa ggaggaccgc acgggcactg gcaccctgtc ggtgttcggc
      241 atgcaggcac gatacagcct gagagatgaa tttcctctgc tcacaaccaa acgagtgttc
      301 tggaagggtg ttttggagga gttgttgtgg tttatcaagg gatccacaaa tgctaaagaa
      361 ttgtcctcaa agggagtgag aatctgggat gccaatggat cccgagattt tctggacagc
      421 ttgggatttt ctgcccgaca ggaaggggac ctgggcccag tttatggttt ccaatggagg
      481 cattttggag cagagtacaa agatatggat tcagattact cgggacaagg agtagaccag
      541 ctgcaaaaag tgattgacac catcaaaacc aaccctgatg acagaagaat catcatgtgt
      601 gcctggaacc caaaagatct tcccctgatg gcactgcctc cttgccatgc cctctgtcag
      661 ttctatgtgg tgaatgggga actgtcttgc cagctttacc agaggtcagg agatatgggt
      721 ctgggcgtgc ccttcaacat tgccagctat gctctgctca cctacatgat tgcacatatc
      781 acaggcctgc agccaggtga ttttgtccac actttgggag atgcacatat ttacctgaat
      841 catatagagc cgctgaaaat tcagctacag cgagaaccaa gacctttccc aaagctcaaa
      901 atccttcgaa aagttgagac aatcgatgat ttcaaagttg aagactttca gattgaaggg
      961 tataatccac atccaacgat taaaatggaa atggctgttt agagtgcttt cagtgatgct
     1021 atgaatatca ccgttttttt ttttttaatt aggtattttc ctcatttaca tttccaatgc
     1081 tataccaaaa gtcccccata ccctcccctc cactccccta cccacccact cccacttctt
     1141 ggccctggca ttcccttgta ctggggcata taaagtttgc aagtccaatg ggcctctctt
     1201 tccagtgatg gccgactagg ccatcttttg atacatatgc agctagagtc aagagctccg
     1261 gggtactggt tagttcataa tgttgttcca cctatagggt tgcagatccc tttagctcct
     1321 tgggtacttt ctctagctcc tccattgggg gccctgtgat ccatccaata gctgacagtg
     1381 agcatccact tctgtgtaca cctccaagag tagccattac agtcactcat ggagtagcgt
     1441 acgaagtact tcaggtagaa gcacttgcag tccctcaagg cagaaccctc gaagtccttc
     1501 aaggatgagc cctcacattc cctcatagag gattctatgc agtccctcaa ggaggagccc
     1561 tcacagtcac tcaatgacca cacacagtcc atcaaggaaa agtcctcgca gtcgttcaag
     1621 gaggaaccct ctaagtcctt caaggatgag ccctcacagt ccctcatgga ggaccccatg
     1681 cagtccctca aggaggagcc ctcacagtta ctcaaggacc caacacagcc catcaaggag
     1741 aagtctttgt agttcctcaa tgtggaaccc acaaagtcct tcaggatgag ccctcacagt
     1801 tcctcatgga ggacctcttg tagtcactca aggaggagcc ctcacagtca cttaagcacc
     1861 ccacacagca catcaaggag aagtccaccc agtccttcca gaaggaaccc tcgaagtcct
     1921 tcaagtatga gccctcacag tccctcatgg aggaccccat gcagtaactt gttgtgagcc
     1981 agctcatggc tcacatggac agggtacacc tgagtggcag gccagggctg aaagagaggg
     2041 gaactaggtg gtcgagagaa aaatggagct aagtcaagat tcctgatcaa agctcaaatg
     2101 tttaatggca catgctctta taaagggggg agggggaggc ccattcccgc caattcattc
     2161 taggagccca gctgcagttg acgacgtgca ggatagggtg aagttccaga atagctcagc
     2221 agcctcccag caggtagcag catcttgaag gggaacagtg gcagtggctg agcaatagag
     2281 tgatctaggg aggaaggctc caccctaggt aatctcctta gtggcagaaa ggtcaatgtc
     2341 tggctcagcc tgcttcaggc tgggggaggt tacaatttcc tccgttatta atattaaata
     2401 agctggactg aggcataaaa tttatgctct atagttctgc ctgatttctt tgggtctagt
     2461 gaagaaacct gtctcagtgg gattccctaa ctttttctca ttgtaaaccc acctattcgc
     2521 taggccatgg tgaataccct tgtctgggta agactggcta ctgtcccaag taaatactct
     2581 gtagactagc cctgagctat tctatctcat tctttgtaat gcctaattag tttcattgtc
     2641 tcttccaagc tcctcggctt caagcatttt ctaggacatt ggaacagtgg ccaaggctta
     2701 gctatgtcag aattcaatct taaaaggcac ttataataag ataatactaa aagagagcac
     2761 gtggagccat acaccagact aacgtgggga tagggtatga gtatacaggt tatgagaaca
     2821 ctaaagttcc aggaggtgag tttctgtgaa aaactctgcc tcgtgagtgc tcccaggcac
     2881 ctctgcctgc caagcaaact tcactgtagt gtgtgtagca ttctccccct ttctttttat
     2941 attcaggagt gctctaaaca caacgattta ctaatagaat agtgccaata atagcaagtt
     3001 gaatatatat atctcccaat ctaggagatt caatgcactt aggttttttt tgtttgtttg
     3061 tttgttttgt tttttatctc tggccaattc atcaatgttc caagtgtcca agtgactttt
     3121 gctaatatct gaaacttctg cctttctagc cttaatatat gctatgtcct gccctgttgt
     3181 taagactgct acttataaga cattaacctt tgcctccaat ttttttacat ggctctgtgg
     3241 tgtattatta gtaaaccacg gcctcttggc atgtgcctct ttttagactg atatgattcc
     3301 gagatctgga tgcccgtctt agactgtctg gccctcatag caaaccttat tcccgattca
     3361 gaccaaaacc acaatatgta cttagaatgc ttcttcatga agatttaata actgccacct
     3421 gtgctgttgg aggtgagggt gcacacctgc tgcagtaagg atgaagggca ggactgacct
     3481 gcttgaaaaa caaagtggag acagtgaaaa gcaacagtac aaaagcttct ttgggggaag
     3541 tccaggtact ccattcaatt gcaaattagc aatggtcatg cttagactta gagctcccgt
     3601 tgtggggagg tgacttcgag aatcacagaa aggctgtaac tttctaggag aggaggctgt
     3661 gctccaaatt aacttttcgg ttaaagagga tttttagctt taataagagt catacttact
     3721 ggaggattca ggatctttat ccagttgaca aactaatctt tcaggcaggc agccatcctg
     3781 ctccatcttc tttttgtg
//
