LOCUS       NM_013711               1922 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus thioredoxin reductase 2 (Txnrd2), transcript variant
            1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_013711 XM_289797
VERSION     NM_013711.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Li,C., Lai,H., Cai,X., Liu,Y., Hong,L., Huang,X. and Shao,L.
  TITLE     Thioredoxin Reductase 2 Synergizes with Cytochrome c, Somatic to
            Alleviate Doxorubicin-Induced Oxidative Stress in Cardiomyocytes
            and Mouse Myocardium
  JOURNAL   Int Heart J 64 (1), 60-70 (2023)
   PUBMED   36725079
  REMARK    GeneRIF: Thioredoxin Reductase 2 Synergizes with Cytochrome c,
            Somatic to Alleviate Doxorubicin-Induced Oxidative Stress in
            Cardiomyocytes and Mouse Myocardium.
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Chocron,E.S., Mdaki,K., Jiang,N., Cropper,J. and Pickering,A.M.
  TITLE     Mitochondrial TrxR2 regulates metabolism and protects from
            metabolic disease through enhanced TCA and ETC function
  JOURNAL   Commun Biol 5 (1), 467 (2022)
   PUBMED   35577894
  REMARK    GeneRIF: Mitochondrial TrxR2 regulates metabolism and protects from
            metabolic disease through enhanced TCA and ETC function.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1922)
  AUTHORS   Kameritsch,P., Singer,M., Nuernbergk,C., Rios,N., Reyes,A.M.,
            Schmidt,K., Kirsch,J., Schneider,H., Muller,S., Pogoda,K., Cui,R.,
            Kirchner,T., de Wit,C., Lange-Sperandio,B., Pohl,U., Conrad,M.,
            Radi,R. and Beck,H.
  TITLE     The mitochondrial thioredoxin reductase system (TrxR2) in vascular
            endothelium controls peroxynitrite levels and tissue integrity
  JOURNAL   Proc Natl Acad Sci U S A 118 (7) (2021)
   PUBMED   33579817
  REMARK    GeneRIF: The mitochondrial thioredoxin reductase system (TrxR2) in
            vascular endothelium controls peroxynitrite levels and tissue
            integrity.
REFERENCE   4  (bases 1 to 1922)
  AUTHORS   Yang,D., Guo,Q., Liang,Y., Zhao,Y., Tian,X., Ye,Y., Tian,J., Wu,T.
            and Lu,N.
  TITLE     Wogonin induces cellular senescence in breast cancer via
            suppressing TXNRD2 expression
  JOURNAL   Arch Toxicol 94 (10), 3433-3447 (2020)
   PUBMED   32671444
  REMARK    GeneRIF: Wogonin induces cellular senescence in breast cancer via
            suppressing TXNRD2 expression.
REFERENCE   5  (bases 1 to 1922)
  AUTHORS   Miranda-Vizuete,A. and Spyrou,G.
  TITLE     Genomic organization and identification of a novel alternative
            splicing variant of mouse mitochondrial thioredoxin reductase
            (TrxR2) gene
  JOURNAL   Mol Cells 13 (3), 488-492 (2002)
   PUBMED   12132591
  REMARK    GeneRIF: Genomic organization and identification of a novel
            alternative splicing variant of mouse mitochondrial TrxR2 gene
REFERENCE   6  (bases 1 to 1922)
  AUTHORS   Lund,J., Chen,F., Hua,A., Roe,B., Budarf,M., Emanuel,B.S. and
            Reeves,R.H.
  TITLE     Comparative sequence analysis of 634 kb of the mouse chromosome 16
            region of conserved synteny with the human velocardiofacial
            syndrome region on chromosome 22q11.2
  JOURNAL   Genomics 63 (3), 374-383 (2000)
   PUBMED   10704284
REFERENCE   7  (bases 1 to 1922)
  AUTHORS   Kawai,H., Ota,T., Suzuki,F. and Tatsuka,M.
  TITLE     Molecular cloning of mouse thioredoxin reductases
  JOURNAL   Gene 242 (1-2), 321-330 (2000)
   PUBMED   10721726
REFERENCE   8  (bases 1 to 1922)
  AUTHORS   Miranda-Vizuete,A., Damdimopoulos,A.E. and Spyrou,G.
  TITLE     cDNA cloning, expression and chromosomal localization of the mouse
            mitochondrial thioredoxin reductase gene(1)
  JOURNAL   Biochim Biophys Acta 1447 (1), 113-118 (1999)
   PUBMED   10500251
REFERENCE   9  (bases 1 to 1922)
  AUTHORS   Sun,Q.A., Wu,Y., Zappacosta,F., Jeang,K.T., Lee,B.J., Hatfield,D.L.
            and Gladyshev,V.N.
  TITLE     Redox regulation of cell signaling by selenocysteine in mammalian
            thioredoxin reductases
  JOURNAL   J Biol Chem 274 (35), 24522-24530 (1999)
   PUBMED   10455115
REFERENCE   10 (bases 1 to 1922)
  AUTHORS   Lund,J., Roe,B., Chen,F., Budarf,M., Galili,N., Riblet,R.,
            Miller,R.D., Emanuel,B.S. and Reeves,R.H.
  TITLE     Sequence-ready physical map of the mouse chromosome 16 region with
            conserved synteny to the human velocardiofacial syndrome region on
            22q11.2
  JOURNAL   Mamm Genome 10 (5), 438-443 (1999)
   PUBMED   10337614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB027566.1 and BF465261.1.
            
            On Apr 17, 2007 this sequence version replaced NM_013711.2.
            
            Summary: The protein encoded by this gene belongs to the pyridine
            nucleotide-disulfide oxidoreductase family, and is a member of the
            thioredoxin (Trx) system. Three thioredoxin reductase (TrxR)
            isozymes are found in mammals. TrxRs are selenocysteine-containing
            flavoenzymes, which reduce thioredoxins, as well as other
            substrates, and play a key role in redox homoeostasis. This gene
            encodes a mitochondrial form important for scavenging reactive
            oxygen species in mitochondria. It functions as a homodimer
            containing FAD, and selenocysteine (Sec) at the active site. Sec is
            encoded by UGA codon that normally signals translation termination.
            The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop
            structure, the Sec insertion sequence (SECIS) element, which is
            necessary for the recognition of UGA as a Sec codon rather than as
            a stop signal. Alternatively spliced transcript variants encoding
            different isoforms have been described for this gene. [provided by
            RefSeq, Jun 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF136399.1, BC029344.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            NMD candidate                      :: translation inferred from
                                                  conservation
            protein contains selenocysteine    :: PMID: 10721726
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1902              AB027566.1         1-1902
            1903-1922           BF465261.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 11.41 cM"
     gene            1..1922
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /note="thioredoxin reductase 2"
                     /db_xref="GeneID:26462"
                     /db_xref="MGI:MGI:1347023"
     exon            1..143
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1612
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /EC_number="1.8.1.9"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     precursor is encoded by transcript variant 1; thioredoxin
                     reductase 2, mitochondrial; thioredoxin reductase TR3; TR
                     beta"
                     /codon_start=1
                     /transl_except=(pos:1604..1606,aa:Sec)
                     /product="thioredoxin reductase 2, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_038739.2"
                     /db_xref="CCDS:CCDS79434.1"
                     /db_xref="GeneID:26462"
                     /db_xref="MGI:MGI:1347023"
                     /translation="MAAMVAAMVAALRGPSRRFRPRTRALTRGTRGAASAAGGQQSFD
                     LLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSPRGTKWGLGGTCVNVGCIPKKLMHQ
                     AALLGGMIRDAHHYGWEVAQPVQHNWKTMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI
                     KASFVDEHTVRGVDKGGKATLLSAEHIVIATGGRPRYPTQVKGALEYGITSDDIFWLK
                     ESPGKTLVVGASYVALECAGFLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHMESHGT
                     QFLKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAG
                     ISTNPKNQKIIVDAQEATSVPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTL
                     MDYSNVPTTVFTPLEYGCVGLSEEEAVALHGQEHVEVYHAYYKPLEFTVADRDASQCY
                     IKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMQTVGIHPTCSEEVV
                     KLHISKRSGLEPTVTGCUG"
     exon            144..209
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            210..266
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            267..411
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            412..486
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..565
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            566..628
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            629..699
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            700..719
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..811
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            812..986
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1123
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1219
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1312
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1384
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1482
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1689
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1905
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1800..1870
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1880..1885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /note="hexamer: AATAAA"
     polyA_site      1902
                     /gene="Txnrd2"
                     /gene_synonym="ESTM573010; TGR; Tr3; Trxr2; Trxrd2"
                     /note="major polyA site"
ORIGIN      
        1 agccctgacc gcctgaggtc cccggaccat ggcggcgatg gtggcggcga tggtggcggc
       61 gctgcgtgga cccagcaggc gcttccggcc gcggacacgg gctctgacac gcgggacaag
      121 gggcgcggcg agtgcagcgg gagggcagca gagctttgat ctcttggtga tcggtggggg
      181 atccggtggc ctagcttgtg ccaaggaagc tgctcagctg ggaaagaagg tggctgtggc
      241 tgactatgtg gaaccttctc cccgaggcac caagtggggc cttggtggca cctgtgtcaa
      301 cgtgggttgc atacccaaga agctgatgca tcaggctgca ctgctggggg gcatgatcag
      361 agatgctcac cactatggct gggaggtggc ccagcctgtc caacacaact ggaagacaat
      421 ggcagaagcc gtgcaaaacc atgtgaaatc cttgaactgg ggtcatcgcg tccaactgca
      481 ggacaggaaa gtcaagtact ttaacatcaa agccagcttt gtggatgagc acacagttcg
      541 cggtgtggac aaaggcggga aggcgactct gctttcagct gagcacattg tcattgctac
      601 aggaggacgg ccaaggtacc ccacacaagt caaaggagcc ctggaatatg gaatcacaag
      661 tgacgacatc ttctggctga aggagtcccc tgggaaaacg ttggtggttg gagccagcta
      721 tgtggcccta gagtgtgctg gcttcctcac tggaattgga ctggatacca ctgtcatgat
      781 gcgcagcatc cctctccgag gctttgacca gcaaatgtca tctttggtca cagagcacat
      841 ggagtctcat ggcacccagt tcctgaaagg ctgtgtcccc tcccacatca aaaaactccc
      901 aactaaccag ctgcaggtca cttgggagga tcatgcttct ggcaaggaag acacaggcac
      961 ctttgacact gtcctgtggg ccatagggcg agttccagaa accaggactt tgaatctgga
     1021 gaaggctggc atcagtacca accctaagaa tcagaagatt attgtggatg cccaggaggc
     1081 tacctctgtt ccccacatct atgccattgg agatgttgct gaggggcggc ctgagctgac
     1141 gcccacagct atcaaggcag gaaagcttct ggctcagcgg ctctttggga aatcctcaac
     1201 cttaatggat tacagcaatg ttcccacaac tgtctttaca ccactggagt atggctgtgt
     1261 ggggctgtct gaggaggagg ctgtggctct ccatggccag gagcatgtag aggtttacca
     1321 tgcatattat aagcccctag agttcacggt ggcggatagg gatgcatcac agtgctacat
     1381 aaagatggta tgcatgaggg agcccccaca actggtgctg ggcctgcact tccttggccc
     1441 caacgctgga gaagtcaccc aaggatttgc tcttgggatc aagtgtgggg cttcatatgc
     1501 acaggtgatg cagacagtag ggatccatcc cacctgctct gaggaggtgg tcaagctgca
     1561 catctccaag cgctccggcc tggagcctac tgtgactggt tgctgaggct aagtcaccat
     1621 ccctgctgag ctaaggacac acagtgtgcc atgtgcccaa tagaaggctc ctcagacacc
     1681 tggatccagg actggaaggc cttgctgcct ctcagagatc tgagaagatg tggatggaac
     1741 atcgtgtgtc ctgcagggat gactataccc ctacattctc tggccagcct ctgacactcc
     1801 cagcgtcaga tgatgatggc ctgggcagaa accccatgtg ggccgcccag gtttgaaccc
     1861 ctggcatttc tagagcacta ataaagagga tgttttacta aagcaaaaaa aaaaaaaaaa
     1921 aa
//
