LOCUS       NM_001042513            3417 bp    mRNA    linear   ROD 09-OCT-2024
DEFINITION  Mus musculus thioredoxin reductase 1 (Txnrd1), transcript variant
            1, mRNA.
ACCESSION   NM_001042513
VERSION     NM_001042513.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Bai,D., Zhou,C., Du,J., Zhao,J., Gu,C., Wang,Y., Zhang,L., Lu,N.
            and Zhao,Y.
  TITLE     TrxR1 is involved in the activation of Caspase-11 by regulating the
            oxidative-reductive status of Trx-1
  JOURNAL   Redox Biol 75, 103277 (2024)
   PUBMED   39059206
  REMARK    GeneRIF: TrxR1 is involved in the activation of Caspase-11 by
            regulating the oxidative-reductive status of Trx-1.
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   O'Connor,C., Keele,G.R., Martin,W., Stodola,T., Gatti,D.,
            Hoffman,B.R., Korstanje,R., Churchill,G.A. and Reinholdt,L.G.
  TITLE     Unraveling the genetics of arsenic toxicity with cellular
            morphology QTL
  JOURNAL   PLoS Genet 20 (4), e1011248 (2024)
   PUBMED   38662777
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3417)
  AUTHORS   Wang,X., Zhang,Y., Yu,J., Ma,Y., Xu,Y., Shi,J., Qi,Z. and Liu,X.
  TITLE     Identification and analysis of key circRNAs in the mouse embryonic
            ovary provides insight into primordial follicle development
  JOURNAL   BMC Genomics 25 (1), 139 (2024)
   PUBMED   38310234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   Hao,X., Zhao,B., Towers,M., Liao,L., Monteiro,E.L., Xu,X.,
            Freeman,C., Peng,H., Tang,H.Y., Havas,A., Kossenkov,A.V.,
            Berger,S.L., Adams,P.D., Speicher,D.W., Schultz,D., Marmorstein,R.,
            Zaret,K.S. and Zhang,R.
  TITLE     TXNRD1 drives the innate immune response in senescent cells with
            implications for age-associated inflammation
  JOURNAL   Nat Aging 4 (2), 185-197 (2024)
   PUBMED   38267705
  REMARK    GeneRIF: TXNRD1 drives the innate immune response in senescent
            cells with implications for age-associated inflammation.
REFERENCE   5  (bases 1 to 3417)
  AUTHORS   Sun,Q.A., Kirnarsky,L., Sherman,S. and Gladyshev,V.N.
  TITLE     Selenoprotein oxidoreductase with specificity for thioredoxin and
            glutathione systems
  JOURNAL   Proc Natl Acad Sci U S A 98 (7), 3673-3678 (2001)
   PUBMED   11259642
REFERENCE   6  (bases 1 to 3417)
  AUTHORS   Sun,Q.A., Zappacosta,F., Factor,V.M., Wirth,P.J., Hatfield,D.L. and
            Gladyshev,V.N.
  TITLE     Heterogeneity within animal thioredoxin reductases. Evidence for
            alternative first exon splicing
  JOURNAL   J Biol Chem 276 (5), 3106-3114 (2001)
   PUBMED   11060283
REFERENCE   7  (bases 1 to 3417)
  AUTHORS   Osborne,S.A. and Tonissen,K.F.
  TITLE     Genomic organisation and alternative splicing of mouse and human
            thioredoxin reductase 1 genes
  JOURNAL   BMC Genomics 2, 10 (2001)
   PUBMED   11737861
REFERENCE   8  (bases 1 to 3417)
  AUTHORS   Rundlof,A.K., Carlsten,M., Giacobini,M.M. and Arner,E.S.
  TITLE     Prominent expression of the selenoprotein thioredoxin reductase in
            the medullary rays of the rat kidney and thioredoxin reductase mRNA
            variants differing at the 5' untranslated region
  JOURNAL   Biochem J 347 Pt 3 (Pt 3), 661-668 (2000)
   PUBMED   10769168
REFERENCE   9  (bases 1 to 3417)
  AUTHORS   Kawai,H., Ota,T., Suzuki,F. and Tatsuka,M.
  TITLE     Molecular cloning of mouse thioredoxin reductases
  JOURNAL   Gene 242 (1-2), 321-330 (2000)
   PUBMED   10721726
REFERENCE   10 (bases 1 to 3417)
  AUTHORS   Sun,Q.A., Wu,Y., Zappacosta,F., Jeang,K.T., Lee,B.J., Hatfield,D.L.
            and Gladyshev,V.N.
  TITLE     Redox regulation of cell signaling by selenocysteine in mammalian
            thioredoxin reductases
  JOURNAL   J Biol Chem 274 (35), 24522-24530 (1999)
   PUBMED   10455115
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ136679.1, AK149625.1,
            AB027565.1 and CJ235434.1.
            
            Summary: The protein encoded by this gene belongs to the pyridine
            nucleotide-disulfide oxidoreductase family, and is a member of the
            thioredoxin (Trx) system. Three thioredoxin reductase (TrxR)
            isozymes are found in mammals. TrxRs are selenocysteine-containing
            flavoenzymes, which reduce thioredoxins, as well as other
            substrates, and play a key role in redox homoeostasis. This gene
            encodes an ubiquitously expressed, cytosolic form of TrxR, which
            functions as a homodimer containing FAD, and selenocysteine (Sec)
            at the active site. Sec is encoded by UGA codon that normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, the Sec insertion sequence
            (SECIS) element, which is necessary for the recognition of UGA as a
            Sec codon rather than as a stop signal. Alternative splicing,
            primarily at the 5' end, results in transcript variants encoding
            same or different isoforms. [provided by RefSeq, May 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes isoform 1. Variants 1-3 encode the same
            isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.755478.1,
                                           SRR7652917.723732.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 10721726
            RefSeq Select criteria          :: based on conservation,
                                               expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               CJ136679.1         2-170
            170-607             AK149625.1         171-608
            608-2110            AB027565.1         528-2030
            2111-3405           AB027565.1         2032-3326
            3406-3417           CJ235434.1         433-444
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 40.66 cM"
     gene            1..3417
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /note="thioredoxin reductase 1"
                     /db_xref="GeneID:50493"
                     /db_xref="MGI:MGI:1354175"
     exon            1..95
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            96..202
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            203..325
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..1738
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /EC_number="1.8.1.9"
                     /EC_number="1.11.1.2"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     is encoded by transcript variant 1; thioredoxin reductase
                     1, cytoplasmic; thioredoxin reductase TR1; TR alpha;
                     peroxidase TXNRD1"
                     /codon_start=1
                     /transl_except=(pos:1730..1732,aa:Sec)
                     /product="thioredoxin reductase 1, cytoplasmic isoform 1"
                     /protein_id="NP_001035978.1"
                     /db_xref="CCDS:CCDS24072.1"
                     /db_xref="GeneID:50493"
                     /db_xref="MGI:MGI:1354175"
                     /translation="MNGSKDPPGSYDFDLIIIGGGSGGLAAAKEAAKFDKKVLVLDFV
                     TPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKVEDTVKHDWEKMT
                     ESVQSHIGSLNWGYRVALREKKVVYENAYGRFIGPHRIVATNNKGKEKIYSAERFLIA
                     TGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTV
                     MVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLRVTAQSTNS
                     EETIEGEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGD
                     ILEGKLELTPVAIQAGRLLAQRLYGGSNVKCDYDNVPTTVFTPLEYGCCGLSEEKAVE
                     KFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKIICNLKDDERVVGFHVLGPNAGEVTQ
                     GFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGCUG"
     exon            326..398
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            399..518
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            519..661
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..777
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            778..1003
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1096
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1173
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1330
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1438
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1534
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1669
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..3417
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1967..2042
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      3385..3390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /note="hexamer: AATAAA"
     polyA_site      3405
                     /gene="Txnrd1"
                     /gene_synonym="TR; TR1; TrxR1"
                     /note="major polyA site"
ORIGIN      
        1 agtttgcttc cgtcaggcct cgcgtccacg cgggaggtgc gggacgccga caccgcgggg
       61 cgagaagagc tggtggtttc accttccttg ttcatagggc ggcggggcct tgcagcggcg
      121 cgggcgagcg gaaaggccgc gggaggcggc gagccagcgg aaggtgcgac cggcggaggg
      181 cggccatggt ccagccctga agccgaacaa aaaaggccaa cttcaaaagc tgccaacaat
      241 gaatggctcc aaagatcccc ctgggtccta tgacttcgac ctgatcatca ttggaggagg
      301 ctcaggagga ctggcagcag ctaaggaggc agccaaattt gacaagaaag tgctggtctt
      361 ggattttgtc acaccgactc ctcttgggac cagatggggt ctcggaggaa cgtgtgtgaa
      421 tgtgggttgc atacctaaga agctgatgca ccaggcagct ttgctcggac aagctctgaa
      481 agactcgcgc aactatggct ggaaagtcga agacacagtg aagcatgact gggagaaaat
      541 gacggaatct gtgcagagtc acatcggctc gctgaactgg ggctaccgcg tagctctccg
      601 ggagaaaaag gtcgtctatg agaatgctta cgggaggttc attggtcctc acaggattgt
      661 ggcgacaaat aacaaaggta aagaaaaaat ctattcagca gagcggttcc tcatcgccac
      721 aggtgagagg ccccgctacc tgggcatccc tggagacaaa gagtactgca tcagcagtga
      781 tgatcttttc tccttgcctt actgcccggg gaagacccta gtagttggtg catcctatgt
      841 cgccttggaa tgtgcaggat ttctggctgg tatcggctta gacgtcactg taatggtgcg
      901 gtccattctc cttagaggat ttgaccaaga catggccaac aaaatcggtg aacacatgga
      961 agaacatggt atcaagttta taaggcagtt cgtcccaacg aaaattgaac agatcgaagc
     1021 aggaacacca ggccgactca gggtgactgc tcaatccaca aacagcgagg agaccataga
     1081 gggcgaattt aacacagtgt tgctggcggt aggaagagat tcttgtacga gaactattgg
     1141 cttagagacc gtgggcgtga agataaacga aaaaaccgga aagatacccg tcacggatga
     1201 agagcagacc aatgtgcctt acatctacgc catcggtgac atcctggagg ggaagctaga
     1261 gctgactccc gtagccatcc aggcggggag attgctggct cagaggctgt atggaggctc
     1321 caatgtcaaa tgtgactatg acaatgtccc aacgactgta tttactcctt tggaatatgg
     1381 ctgttgtggc ctctctgaag aaaaagccgt agagaaattt ggggaagaaa atattgaagt
     1441 ttaccatagt ttcttttggc cattggaatg gacagtccca tcccgggata acaacaaatg
     1501 ttatgcaaaa ataatctgca accttaaaga cgatgaacgt gtcgtgggct tccacgtgct
     1561 gggtccaaac gctggagagg tgacgcaggg ctttgcggct gcgctcaagt gtgggctgac
     1621 taagcagcag ctggacagca ccatcggcat ccacccggtc tgtgcagaga tattcacaac
     1681 gttgtcagtg acgaagcgct ctgggggaga catcctccag tctggctgct gaggttaagc
     1741 cccagtgtgg atgctgttgc caagactaca gaccattgcc ttgcttcctt gcccacgccc
     1801 aggtgaagtt caggaaggct cttgggtcct aggcgccaat tcaaggtgct gtcctaaggc
     1861 caccgggtcc ctgggatctt gtgggtagga ggtggcaggt cgaaggaggc tgcagcatcg
     1921 cactggggtc accatgacag actcagactg acatctggca gagcatcaca ggcatgcgtc
     1981 catgaagtca ctggcctcaa gcccaagtgg tgggcagtga cagaagagct gccgggtctg
     2041 ttgagctcaa ccttttcctg tagattgtct tagtctcact ttcaagctgt ctaatgtcaa
     2101 ttctgttttt cttttttcct ccatggggtt aatgatacta gagataggga atattagcaa
     2161 tcagtttttg tcatggctgg tccatctgca acagtcttta ctgtgtggaa gtgggtgaga
     2221 tggcttatga gagccaaacc aatttatccc cagaaagacg aattaccctg tgactaaaat
     2281 acactgtctg cttttactaa ctggtgtagc attgtctcct ttaataagtc ttgtgtccaa
     2341 aacgagaaaa accattggcc acttttgcaa gtttcctgca gtgtgcttag caagggaggt
     2401 ggcgacttgg ctaatctact tgaactgcat cgcatggctc ttgggtagct tagagcatcg
     2461 cagggtagag gcagaccagc agtgagtgtc tctcctggta caattattgt ctggttctca
     2521 gtggaaaacg cttaatttgc tttaaacttg gtgtttttgt gaggtggatt tagtcttaag
     2581 ctgtgtccca taagaactac attcacaggc aagtggctct tcctccacac agcctataca
     2641 tcttctgagg taattacttt cataaggaag ctgttcataa cgtaagttat tattattatt
     2701 gaacacaggt ggatgtgaag gatttttcat tgaaaaacca aatggttttt ctttttttct
     2761 gttcagtgag cccacaggaa ctctgtcagg acagccagta ctctgccggc atggctgctg
     2821 gggcgtttac ggtgtagttt agctcctagg ttacatgacc gtgaacatgc tggctgagga
     2881 ctacacaaac caggtttccc accatacacg gcctggccct gcagcttctt ttcttgccct
     2941 cccctttgcc tgtccccacc tgcagtactg agtggcgttt cacagtaccc ttctgggcca
     3001 cctcagggaa gggatttgcc tggtgtccag ccagcagcac ccaccctgcc ccacgaggct
     3061 ccctcacacc tgcccccccg tccttgtgtt gaagacagtg ggaagaggag aaaggaccag
     3121 ggaaaccaag ggagttgact gttcagtttt atttatttat tttttaagtt tttttttcct
     3181 ttcaagtctg ccagtctctg agatcagaac aacagacagt gtagggtaac taatcatgtg
     3241 attctcttag tggaatgaaa tgttctaccc ccacaagaag gagtatacgt cattgttcat
     3301 attctgtaat cgcacaatgt attgtaatgc aaattccaat tccatgtatt tttattacaa
     3361 tttttctgga aaaaaatgtg aaccaataaa agatgttgat gcacacgcgt gccttct
//
