LOCUS       NM_001146002            1892 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus thioredoxin domain containing 2 (spermatozoa)
            (Txndc2), transcript variant 1, mRNA.
ACCESSION   NM_001146002
VERSION     NM_001146002.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Smith,T.B., Baker,M.A., Connaughton,H.S., Habenicht,U. and
            Aitken,R.J.
  TITLE     Functional deletion of Txndc2 and Txndc3 increases the
            susceptibility of spermatozoa to age-related oxidative stress
  JOURNAL   Free Radic Biol Med 65, 872-881 (2013)
   PUBMED   23707457
  REMARK    GeneRIF: Txndc2 and Txndc3 are critically important in protecting
            spermatozoa against the increases in oxidative stress associated
            with paternal age.
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Jimenez,A., Prieto-Alamo,M.J., Fuentes-Almagro,C.A., Jurado,J.,
            Gustafsson,J.A., Pueyo,C. and Miranda-Vizuete,A.
  TITLE     Absolute mRNA levels and transcriptional regulation of the mouse
            testis-specific thioredoxins
  JOURNAL   Biochem Biophys Res Commun 330 (1), 65-74 (2005)
   PUBMED   15781233
REFERENCE   3  (bases 1 to 1892)
  AUTHORS   Aitola,M.H. and Pelto-Huikko,M.T.
  TITLE     Expression of Arnt and Arnt2 mRNA in developing murine tissues
  JOURNAL   J Histochem Cytochem 51 (1), 41-54 (2003)
   PUBMED   12502753
REFERENCE   4  (bases 1 to 1892)
  AUTHORS   Yu,Y., Oko,R. and Miranda-Vizuete,A.
  TITLE     Developmental expression of spermatid-specific thioredoxin-1
            protein: transient association to the longitudinal columns of the
            fibrous sheath during sperm tail formation
  JOURNAL   Biol Reprod 67 (5), 1546-1554 (2002)
   PUBMED   12390887
REFERENCE   5  (bases 1 to 1892)
  AUTHORS   Jimenez,A., Oko,R., Gustafsson,J.A., Spyrou,G., Pelto-Huikko,M. and
            Miranda-Vizuete,A.
  TITLE     Cloning, expression and characterization of mouse spermatid
            specific thioredoxin-1 gene and protein
  JOURNAL   Mol Hum Reprod 8 (8), 710-718 (2002)
   PUBMED   12149401
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166058.2.
            
            On Apr 8, 2022 this sequence version replaced NM_001146002.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060981.1, SRR5189685.213341.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AC166058.2         178065-178283       c
            220-365             AC166058.2         175643-175788       c
            366-1892            AC166058.2         173591-175117       c
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 34.99 cM"
     gene            1..1892
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /note="thioredoxin domain containing 2 (spermatozoa)"
                     /db_xref="GeneID:213272"
                     /db_xref="MGI:MGI:2389312"
     exon            1..219
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /inference="alignment:Splign:2.1.0"
     CDS             215..1867
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spermatid-specific thioredoxin 1; thioredoxin
                     domain-containing protein 2; thioredoxin-4"
                     /codon_start=1
                     /product="thioredoxin domain-containing protein 2 isoform
                     1"
                     /protein_id="NP_001139474.1"
                     /db_xref="CCDS:CCDS50165.1"
                     /db_xref="GeneID:213272"
                     /db_xref="MGI:MGI:2389312"
                     /translation="MDLAIEEMFKKDKQKNKDKGLDMNSVQAGAPEESDMTLNNGGKA
                     NERGSNENPLQALSKNEAFLVPEFLDTAQSKEKAIASKVSNTLHMSTEESEFPQQVSS
                     TPMFSENTVHPRHEVSPKPSSKNTQLKQENISKSSGYSKQTNYSNTPKSLAKTTHPKQ
                     GSTLKPATNSTHYREDDIPKSSEDIIQPKKGDRPKSSEDIIQSKKEDRPKSSEDIIQS
                     KKEDRPKSSEDIIQSKKEDRPKSSEDIIQSKKEDRPKSSEDIIQPKKEDRPKSSEDSV
                     PSKKGDRPKSSEDSVQPKKEDRPKSSEDSVQSKEGEVHKPLKDSIQSKETKVPKSPQD
                     SIQSKEDKTHRPLKDSVQSKESEEPKSSHESIQSKEDKIHKPLKDSIPSKEGDIPKSP
                     EDTIQSQEEITASEEDTIQSQEGNTIKSSEEDVQLSESKLLGLGAEIETLEEGLVRVI
                     KDKEEFEEVLKDAGEKLVAVDFSAAWCGPCRMMKPLFHSLSLKHEDVIFLEVDTEDCE
                     QLVQDCEIFHLPTFQFYKNEEKVGEFSGALVGKLERSISELK"
     exon            220..365
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /inference="alignment:Splign:2.1.0"
     exon            366..1892
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1858..1863
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /note="hexamer: ATTAAA"
     polyA_site      1892
                     /gene="Txndc2"
                     /gene_synonym="Sptrx-1; Sptrx1; Trx4"
                     /note="major polyA site"
ORIGIN      
        1 atatcaaccc tcctctacaa ggctcctggg accatcctag aagagaggag aagaaggatg
       61 cacgtgatag gggaaggaat gctgtgagat gctgtctcct ggctgtggca ctcacggact
      121 cacagccgcc acacaagatc aaaccagcaa gaccagccag cgttccaacc gacagcgcta
      181 atcggactca gtgggctgca gaaaagagac ggacatggac ttggccatag aagaaatgtt
      241 taagaaggac aaacaaaaga ataaggacaa ggggctggac atgaacagtg ttcaagcagg
      301 agctcctgaa gagtcggaca tgacactcaa caacggagga aaagcaaatg aaaggggcag
      361 caacgaaaat ccattacagg ctttatccaa aaatgaggct tttctggtcc cagagttctt
      421 ggatacagcc cagtccaaag agaaggccat tgcttccaag gtcagcaata cacttcatat
      481 gtccacagaa gagtctgagt tcccgcagca agttagtagc acccccatgt tttcagagaa
      541 taccgtccat cccagacatg aagttagccc caagccttca tcaaaaaaca ctcagctgaa
      601 acaggaaaat atttctaaat cctcgggcta ctccaagcag accaactata gcaacactcc
      661 caagtccttg gcaaaaacca cccatcccaa acaggggagc accctcaagc ctgcaacgaa
      721 cagcacccat tacagggagg atgacattcc caagtcctca gaagacatca tccaacccaa
      781 gaaaggggac aggcccaagt cctcagaaga catcatccaa tccaagaaag aggacaggcc
      841 caagtcctca gaagacatca tccaatccaa gaaagaggac aggcccaagt cctcagaaga
      901 catcatccaa tccaagaaag aggacaggcc caagtcctca gaagacatca tccaatccaa
      961 gaaagaggac aggcccaagt cctcagaaga catcatccaa cccaagaaag aggacaggcc
     1021 caagtcctca gaagacagcg tcccatccaa gaaaggggac aggcccaagt cctcagaaga
     1081 cagcgtccaa cccaagaaag aggacaggcc caagtcctca gaagacagcg tccaatccaa
     1141 ggaaggtgaa gtccacaagc ccttaaaaga cagcattcag tccaaggaga ctaaagtccc
     1201 taagtcccca caggactcaa tccagtccaa agaggataaa acccacaggc ccttaaagga
     1261 cagcgttcag tcaaaggaga gtgaagaacc taagtcctcc catgaatcca tccagtccaa
     1321 ggaggataaa atccataagc ctttaaaaga tagcatccca tctaaggagg gagatattcc
     1381 caagtcccca gaagatacca tccagtccca ggaagaaatc accgcgtccg aagaagacac
     1441 catccagtcc caggaaggta acactatcaa gtcttcagaa gaagatgtgc agctctcaga
     1501 gagcaaactc ttaggccttg gagcagaaat agagaccctg gaggaaggct tggtgagagt
     1561 gatcaaagac aaggaggaat ttgaggaggt gctcaaagac gctggagaga agctggtggc
     1621 tgtggatttc tcagccgctt ggtgtggccc ctgcagaatg atgaagccac tcttccattc
     1681 cctgtctttg aagcacgagg atgtgatatt cttggaggtg gacactgagg attgtgagca
     1741 gctggtgcaa gactgtgaga tctttcacct cccgactttc cagttttata aaaacgaaga
     1801 aaaggtgggt gaattttctg gtgcccttgt gggaaaactc gaaagaagca tttcagaatt
     1861 aaagtaatca tatgttctga gaacactgtc aa
//
